Labshake search
Citations for Millipore Sigma :
1551 - 1600 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... two initial doses of diphtheria toxin (C. diphtheria, Sigma Cat#D0564-1MG) 18 ng/g bodyweight per day were given before adoptive cell transfer ...
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SIRT1 ameliorates premature senescence-induced defenestration in hepatic sinusoidal endothelial cellbioRxiv - Cell Biology 2020Quote: The reagents used included carbon tetrachloride (Sigma-Aldrich, 56-23-5), 30%H2O2 (Hydrogen peroxide 30% ...
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bioRxiv - Biochemistry 2020Quote: PDP-23 was mixed with human pooled male serum (Sigma-Aldrich) to a final concentration of 50 µg/mL and incubated at 37 °C ...
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bioRxiv - Plant Biology 2019Quote: ... dissolved in tap water or 0.01 % Direct Red 23 (Sigma-Aldrich) dissolved in 1× PBS ...
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bioRxiv - Biochemistry 2021Quote: The main materials purchased were angiotensin (Sigma, Cat #05-23-0101), 3-methylene-2-norbornanone (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... at a ratio of 177:100:23 in PBS (D8537, Sigma). The volume of PBS added to the polyacrylamide mix was controlled to produce a gel of a specific stiffness while having a minimal effect in the chemical properties of the gels across conditions (10.6% polyacrylamide in PBS v/v for 0.1 kPa ...
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bioRxiv - Cell Biology 2022Quote: ... with 2.5% 1,4-23 diazabicyclo[2.2.2]octane (DABCO, Sigma-Aldrich D27802) and examined with a CLSM Nikon A1 (Nikon ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated with one or more of the following primary antibodies overnight at 4°C: AGO2 1:1500 dilution (Sigma-Aldrich SAB4200085 Lot#087M4841V), GW182 1:10,000 dilution (Bethyl A302-329A-T Lot#A302-329-T-2) ...
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bioRxiv - Immunology 2019Quote: ... Membranes were blocked for 1 hour in milk block solution and then incubated overnight at 4°C with primary antibodies for either β-actin (Sigma A5441, 1:2000), IL-1β (R&D AF401NA ...
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bioRxiv - Bioengineering 2021Quote: ... and heated to 90°C.[8] The samples were run, transferred, and probed with 1:500 dilution of primary antibodies (ALIX, CD9, TSG101 (Millipore Sigma, Burlington, MA, USA), calnexin (Cell Signaling Technology Inc. ...
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bioRxiv - Neuroscience 2020Quote: ... Flat-mounted retinas were incubated overnight at 4°C with antibodies against RNA-binding protein with multiple splicing (RBPMS; EMD Millipore Corp., Burlington, MA) diluted 1:500 in blocking buffer (2% bovine serum albumin ...
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bioRxiv - Cell Biology 2021Quote: ... Primary and secondary antibody incubations were performed for 1 hour at 25°C in blocking buffer with anti-Flag-M1 (Sigma-Aldrich, #F3040, 1:1000) conjugated with Alexa-647 (Molecular Probes ...
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bioRxiv - Biochemistry 2022Quote: ... The diluted lysate was incubated with antibody overnight at 4 °C with rotation: H3K4me3 (4 × 106 cells, 2.5 μg anti-H3K4me3, Millipore, Cat#07-473, Lot#3394198), H3K36me2 (10 × 106 cells ...
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bioRxiv - Cell Biology 2022Quote: ... catalog # BP151-500) in PBS for 1 hour followed by overnight incubation at 4°C with the γ-H2AX antibody (Millipore Sigma, catalog # 05636I) using a 1:150 dilution and the H3K9me3 antibody (Millipore Sigma ...
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bioRxiv - Pathology 2020Quote: ... and 0.1% Triton-X 100 in PBS) and stained overnight at 4° C with antibodies against PRG2 (1:1000 dilution, rabbit; Sigma-Aldrich cat. no. HPA038515), HLA-G (1:10,000 dilution ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were incubated in primary antibody overnight at 4° C (chick α-GFP, Abcam, Cat# AB13970, Lot# GF305729-1; mouse α- γ-tubulin, Sigma-Aldrich, Cat# T6557 ...
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bioRxiv - Neuroscience 2020Quote: ... in TBST before incubating overnight at 4°C in primary antibody solution (4% NGS in TBST) with rat anti-GFAP (Millipore Cat# 345860-100UG, RRID:AB_211868), rabbit anti-GFAP (1:2000 ...
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bioRxiv - Developmental Biology 2020Quote: ... Standard IF staining was carried out by incubation of tissue sections with primary antibodies overnight at 4 °C at the following dilutions: mouse anti-acetylated tubulin (Sigma cat. # T7451; 1:1000), mouse anti-RAB11 (BD Transduction Laboratories cat ...
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bioRxiv - Genomics 2021Quote: ... 5% normal donkey serum in PBS for 1 hour and incubated at 4°C overnight with primary antibody (EMD Millipore, cat. Ab3786, 1:300), then at room temperature for 1 hour with Alexa Fluor 594-conjugated secondary antibody (Life Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... at 20°C overnight and then treated with the secondary antibody (AlexaFluor 488-conjugated goat anti-mouse IgG; Sigma-Aldrich; 1:1000 in PBS) at 20°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were then co-labelled overnight at 4°C with primary antibodies directed against Paired Box 7 (Pax7; DSHB; 1:50) and Laminin α1 (Sigma-Aldrich #L9393; 1:100) in 2% BSA ...
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bioRxiv - Microbiology 2022Quote: ... Neurons were stained with the following primary antibodies overnight at 4°C: mouse monoclonal anti-β-III-Tubulin (Tuj1, 1:1000; Millipore cat. No. MAB5564) and rabbit polyclonal anti-SARS-CoV-2 (COVID-19 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in primary antibodies (diluted in the blocking solution, see Table 1) against myc (rabbit c-Myc, 1:1000, Sigma-Aldrich, C3956, RRID: AB_439680) and FLAG (mouse-FLAG ...
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bioRxiv - Molecular Biology 2022Quote: ... Sections were then incubated with the following primary antibodies diluted 1:200 in PBS overnight at 4°C: β-spectrin non-erythrocyte (hpa012685, Sigma, St. Louis, MO), collagen type II (ab34712 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK (1:1000, Sigma Millipore, C2581; Frontier Institute, Af350), rabbit polyclonal anti-GABA (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... The rest of the pre-cleared lysates was then incubated overnight at 4°C with monoclonal rabbit Anti-HA Tag antibodies (Millipore, clone 114-2C-7) at a 1:400 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... in PBS-T (1× PBS and 0.1% Tween 20) for 1 hour at room temperature and incubated overnight at 4°C with primary antibodies staining for FLAG (Sigma Cat# F1804, 1:500) and DDX50 (Proteintech Cat# 10358-I-AP ...
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bioRxiv - Genomics 2023Quote: ... Primary antibodies were applied overnight at 4°C and included TH (Pel-Freez Biologicals #P40101 and Merck Millipore #AB9702, both at 1:750 dilution), MAP2 (Santa Cruz #sc-74421 ...
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bioRxiv - Immunology 2022Quote: ... and then incubated for 1 hour at 37°C with secondary goat anti-rabbit antibodies conjugated with fluorescein isothiocyanate (FITC; 1:250; Sigma-Aldrich, Prague, Czech Republic). Fluorescence intensity was measured using the Synergy H1 microplate reader (BioTek ...
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bioRxiv - Cell Biology 2023Quote: ... blocked with 5 % non-fat dry milk in TBST (TBS with 0.1 % Tween-20) and probed at 4 °C overnight with the following primary antibodies: mouse monoclonal anti-FLAG M2 (Sigma-Aldrich F3165, 1:1000), mouse monoclonal anti-α-tubulin B512 (Sigma-Aldrich T5168 ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then co-labelled overnight at 4°C with primary antibodies directed against Paired Box 7 (Pax7; DSHB; 1:50) and Laminin α1 (Sigma-Aldrich #L9393; 1:100) in 2% BSA ...
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bioRxiv - Cell Biology 2023Quote: ... followed by immediate fixation with 4% paraformaldehyde in PBS at 37⁰C and then immunostaining with anti-puromycin antibody (Sigma Aldrich, clone 12D10, MABE343).
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bioRxiv - Cell Biology 2024Quote: ... then salivary glands were incubated for 24 hours at 4°C with the following primary antibodies: anti-pan-PMCA (5F10, MABN1802, Sigma-Aldrich, dilution 1:250), anti-Dlg (4F3 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Membranes were incubated overnight at 4°C with primary antibodies probing Spectrin and enzyme-mediated degradation products (mouse anti-Spectrin, 1:1000, Millipore Sigma, Cat. No. MAB1622) and early growth response protein-1 (mouse anti-Egr-1 ...
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bioRxiv - Developmental Biology 2024Quote: ... They were then incubated overnight at 4°C with primary antibodies in blocking solution: rabbit anti-GFP (MBL Cat# 598; 1/300) and mouse anti-HA (Sigma, Cat# 12ca5; 1/50). On the next day ...
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bioRxiv - Immunology 2021Quote: ... Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore). Cytokines in mouse BALF were analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453 ...
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bioRxiv - Immunology 2022Quote: ... Cytokine/chemokine measurements in sera were performed using U-PLEX Adipokine assays (Meso Scale Discovery, Millipore).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-10 concentrations were determined with MILLIPLEX MAP Porcine Cytokine/Chemokine Magnetic Bead Panel (Merck-Millipore) with strict adherence to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... chemokines were preincubated with a 103-fold molar excess of soluble heparin (Sigma, St. Louis, MO). Chemokine samples were added to the bottom chambers of 96-well ChemoTx plates (Neuroprobe ...
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bioRxiv - Cell Biology 2023Quote: ... Ten µL of ligand (10-5 M isoproterenol or Thrombin (Sigma Cat# T4648) at 100 U/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.15×106 of murine stromal cells expressing human delta like ligand 1 (Sigma) were combined with 7.5×103 of sorted and enriched KO-/- ...
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bioRxiv - Neuroscience 2023Quote: ... 100 nanomoles of JF552-Halotag ligand were dissolved in 20μl of DMSO (Sigma), diluted first in 20 μl of Pluronic™ F-127 (20% w/v in DMSO ...
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bioRxiv - Plant Biology 2019Quote: ... 16 h dark 19°C) seedlings were transferred to sterile Magenta vessels (Sigma) and grown for 4-5 weeks until harvesting rosette leaves for protoplast isolation ...
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bioRxiv - Cell Biology 2020Quote: ... cells were dissociated using accutase at 37°C for 10 min (Sigma-Aldrich) and 450-600,000 cells were seeded per each well of a 6-well plate ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated at 37°C with 1mg/ml FITC-DEXTRAN (Sigma) for 1h ...
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bioRxiv - Developmental Biology 2021Quote: ... Discs were washed twice with 1 ml cell dissociation buffer (Sigma, C-1544). Elastase (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Boston University) were grown at 24 °C in Schneider’s insect media (Sigma-Aldrich) supplemented with 20% heat-inactivated fetal bovine serum (Corning) ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were digested again overnight at 37°C with trypsin (Sigma, St Louis) at a ratio of 1:10 ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 hour at 37°C and then overnight with Laminin (Sigma Aldrich) at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... + 10% Tet-Free FBS (Takarabio 631101) + 1x pen/strep (Millipore TMS-AB2-C)) on an uncoated T75 flask ...