Labshake search
Citations for Millipore Sigma :
1351 - 1400 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... The sections were incubated for 48 h at 4°C with primary mouse anti-TH antibody (anti-mouse-TH; MAB-318, 1:1000; Millipore, Temecula, CA). The sections were then incubated for 1 h with the appropriate secondary antibody after which sections were washed with PBS and incubated with the required detection kits for development ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were washed in TBS-T and incubated overnight at 4 °C in the same buffer with anti-TH monoclonal antibodies (1:500; Millipore, Stockholm, Sweden). Immunoreactivity was detected using a secondary antibody conjugated with Alexa fluorescent dye (1:400 ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein was transferred to 0.45 µm nitrocellulose membranes prior to overnight incubation at 4°C with the following primary antibodies: mouse anti-ATXN3 (1H9; 1:1000, MAB5360; Millipore, Billerica, MA), mouse anti□GAPDH (1:10000 ...
-
bioRxiv - Microbiology 2023Quote: ... COMMD2 immunoblots were blocked for 1 hr at room temperature in 3% milk TBST and probed overnight at 4°C with the primary antibody (Sigma ref# HPA044190) suspended in 3% milk TBST ...
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked in 5% non-fat milk/TBST for one hour at room temperature and incubated overnight at 4°C with the following primary antibodies diluted in blocking reagent: 1 μg/mL mouse α-alpha-tubulin (DM1A, EMD Millipore), 1 μg/mL rabbit α-LEM-2 (Novus Biologicals) ...
-
bioRxiv - Biophysics 2022Quote: ... Staining is performed overnight at 4 °C for α- and β-tubulin using a mixture of three primary antibodies (T5168, T6199, T5923, Sigma) at a combined dilution of 1:150 in blocking buffer ...
-
bioRxiv - Neuroscience 2023Quote: ... for 1 hr at RT and blotted overnight at 4°C in the same blocking solution with the following antibodies: Rabbit Anti-GluK1 (1:1000; Millipore: 07-258), Rabbit Anti-GluK2/3 (1:1000 ...
-
bioRxiv - Neuroscience 2023Quote: ... with 0.2% triton X-100 and for 12 h at 4 °C in a humidified chamber with the primary antibody: mouse-anti-NeuN (Neuronal Nuclei, Sigma-Aldrich MAB377) or goat-anti-MMP-9 (Matrix metallopeptidase 9 ...
-
bioRxiv - Physiology 2023Quote: ... Membranes were blocked in 1% Casein/PBS (1 hour, room temp) and incubated with the following primary antibodies (4°C, overnight): anti-Nup93 (1:1000, Sigma-Aldrich, HPA017937); anti-Nup107 (1:200 ...
-
bioRxiv - Physiology 2024Quote: ... muscle sections were incubated overnight at 4°C with a primary antibody against laminin (Millipore-Sigma, Cat. No. L9393, 1:100 dilution) to label the basal lamina surrounding myofibers ...
-
bioRxiv - Physiology 2024Quote: ... muscle sections were incubated overnight at 4°C with a primary antibody against laminin (Millipore-Sigma, Cat. No. L9393, 1:100 dilution) to label the basal lamina surrounding myofibers ...
-
bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4°C with the corresponding primary antibodies diluted in blocking buffer: mouse anti-β-Actin 1:2000 (Sigma, #A544); mouse anti-3R-Tau 1:1000 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Chromatin from about 1.5×106 cells was incubated overnight at 4°C with 2–5 μg antibodies to FLAG epitope (M2; Sigma-Aldrich, #F1804, RRID:AB_262044), BRD4 (Cell Signaling Technology ...
-
bioRxiv - Neuroscience 2023Quote: ... slices were incubated overnight at 4°C in blocking solution with the addition of the following antibodies (dependent on experiment): goat anti-ChAT (Millipore Sigma AB144P), rabbit anti-cFos (Synaptic System 226 008) ...
-
bioRxiv - Neuroscience 2023Quote: ... for 2 hours at RT and then immunoblotted overnight at 4°C with the primary mouse anti-amyloid precursor protein A4 antibody (clone 22C11) (Sigma MAB348-AF647) (1:3000 ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were washed 3 times with PBS and incubated with blocking solution (PBS/BSA 1%) for 1 h at 37 °C and then with the following primary antibodies: mouse anti-GFAP (1:500; Millipore, Cat. MAB360), rabbit anti-C3 (1:25 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were blocked with goat serum diluted 1:200 in PBS for one hour followed by an overnight incubation at 4°C with the primary antibody (HIF-1α, 1:200, described in32; BACH-1, 1:200, Sigma Aldrich HPA034949) Next ...
-
bioRxiv - Plant Biology 2024Quote: ... The membrane was incubated 2h at 4°C with ANTI-FLAG antibodies® M2-peroxydase (Sigma-Aldrich, used at 1/ 1000 dilution). The antibodies were detected by using Lumi-Light Western Blotting Substrate (Roche) ...
-
bioRxiv - Immunology 2023Quote: ... Membranes were blocked in TBS-T with 5 % non – fat milk for 1 hour in RT following incubation overnight at 4 °C with primary (1:1000) antibodies: Flag (Sigma, Cat. F1804), IL-1β (Cell Signaling Technology ...
-
bioRxiv - Neuroscience 2024Quote: ... before being incubated overnight at 4°C with mouse anti-TH antibody (1:8000 dilution, T2928, Sigma-Aldrich, St. Louise, MO, USA), 1% NHS ...
-
bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
-
bioRxiv - Neuroscience 2024Quote: ... 75 μg of sample was incubated at 4°C overnight on rotator with 5 μg mouse anti-puromycin antibody (Millipore Sigma: MABE343), or rabbit anti-eIF4E (Abcam ...
-
bioRxiv - Neuroscience 2024Quote: ... and probed with the following antibodies (diluted in 5% milk in PBST) overnight at 4 °C: α-Tubulin (1:400000, Sigma t9026), α-Dnmt3a (1:5000 ...
-
bioRxiv - Biochemistry 2024Quote: ... MIB1 knockout was confirmed by TOPO cloning of the amplified genomic sequence and by the loss of MIB1 signal on western blot with antibodies for the N- and C-terminal portions of MIB1 (Abcam, ab124929; Millipore Sigma, M5948).
-
bioRxiv - Biochemistry 2024Quote: ... centrifuged at 20 000 rpm for 30 min at 4 °C and supernatants were analyzed by Western Blot using monoclonal ANTI-FLAG M2-Peroxidase (HRP) antibody (Sigma-Aldrich, A8592).
-
Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cellsbioRxiv - Cell Biology 2020Quote: ... oligo(dT)23 and M-MLV reverse transcriptase (Sigma-Aldrich) were used according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ND7/23 cells (ECACC 92090903, Sigma-Aldrich/ Merck, Darmstadt, Germany) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM ...
-
bioRxiv - Developmental Biology 2022Quote: ... sections were stained with 0.05% Direct Red 23 (Sigma 212490) in ClearSee for 30 minutes ...
-
bioRxiv - Microbiology 2020Quote: ND7/23 cell culture (ECACC 92090903, Sigma-Aldrich, Munich, Germany) and electrophysiological experiments were performed as described elsewhere 31,32 ...
-
bioRxiv - Plant Biology 2022Quote: ... then added 0.1% Direct red 23 (w/v) (Sigma-Aldrich) into vials and applied vacuum 3 times for 10 min each ...
-
bioRxiv - Biochemistry 2024Quote: ... After adding tricosanoic acid (FA 23:0) (Sigma-Aldrich, USA) as an internal standard ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 100 μl cold Antibody Buffer and 1□0μl antibody (H3K4me3, EpiCypher #13-0041; H3K27Ac, Active Motif #39133; SOX11, Sigma #HPA000536 or HNRNPH1 Abcam #ab154894) was added for incubating on nutator overnight at 4□°C ...
-
bioRxiv - Pathology 2021Quote: ... brain sections were blocked with a PBS solution containing 0.1% Triton X-100 and 10% goat serum to be incubated with a monoclonal goat anti-Iba1 antibody overnight at 4 °C (1:250 - Millipore, Temecula, CA, USA) followed by a donkey anti-goat Alexa Fluor 488 antibody (1:500 - Millipore ...
-
bioRxiv - Immunology 2021Quote: ... for 1-2 hours and then probed overnight at 4 °C with primary antibodies (all diluted 1:1000 unless noted otherwise): Mouse β-actin (Sigma; A-1798), pro and mature IL-1β (R&D Systems ...
-
bioRxiv - Developmental Biology 2022Quote: Samples were incubated with primary antibody overnight at 4°C in PBT (PBS with 3% Triton X-100 (Sigma-Aldrich X100-100 mL)) with 5% NGS (Capralogics GS0250) ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cells were incubated with the primary antibody overnight at 4 C (Anti-Histone γ-H2AX, #07-627 clone PC130, Millipore Sigma 1:1000) and the secondary antibody (Goat anti-Mouse Alexa Fluor 647 Polyclonal ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... 0.1% DMSO in 1x PBS) and placed overnight (16h) at 4°C in 30 µL anti-phospho-histone H3 (Ser10) primary antibody (Millipore Sigma, Burlington, MA) diluted 1:100 in blocking solution ...
-
bioRxiv - Animal Behavior and Cognition 2019Quote: ... The following primary antibodies were applied for 24 h (or 48 h for anti-cFos antibodies) at 4°C: mouse anti-APC/CC1 Ab-7 (Millipore, OP80, 1:100), chicken anti-mCherry (Abcam ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were incubated in PBS containing 0.02% Triton X-100 and 2% NDS overnight at 4°C with primary antibodies: anti-ChAT (1:500, goat polyclonal, Millipore Cat# AB144P, RRID:AB_2079751) and anti-phosphorylated ribosomal protein S6 (p-rpS6240/244 ...
-
bioRxiv - Neuroscience 2019Quote: ... for 15 min at room temperature and stained overnight with the primary antibodies at 4 °C (Kinesin heavy chain: Millipore MAB1614 1:50; Poly-GR: Millipore MABN778 1:50). The following day ...
-
bioRxiv - Synthetic Biology 2021Quote: ... non-fat milk in TBST buffer (Tris-buffered saline containing 0.05% Tween-20) overnight at 4 °C and incubated with anti-FLAG antibody (Sigma, F1804, 1:1000 dilution) or anti-HA antibody (Abcam ...
-
bioRxiv - Microbiology 2020Quote: ... and probed over-night at 4°C either in TBS-TM with a 1:1000 anti-Flag antibody (Sigma-Aldrich; catalogue No F3165), or with a 1:10000 anti-LpoA antibody (loading control 28 ...
-
bioRxiv - Cell Biology 2019Quote: ... the samples were incubated overnight at 4°C with the following primary antibodies: mouse anti-DCP2 (Sigma, Saint Louis, MI, USA; 1:20), rabbit anti-LSm4 (Sigma ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... blots were incubated overnight at 4 °C with the corresponding primary antibody: anti-phospho-histone 2AX (Ser139) (EMD Millipore, 05-636, 1:1000), anti-phospho-Akt (Cell Signaling ...
-
bioRxiv - Microbiology 2021Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with monoclonal rabbit Anti-HA Tag antibodies (Millipore, clone 114-2C-7) at 1:400 dilutions ...
-
bioRxiv - Neuroscience 2021Quote: ... nonspecific staining was blocked with blocking solution (TBST and 4% normal donkey or normal goat serum) and incubated overnight at 4°C with SOX9 antibody (1:2000, Millipore, AB5535, RRID: AB_2239761). Sections were washed and incubated with Alexa 568-conjugated secondary antibody (1:5000 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated overnight at 4°C in primary antibodies (anti-lamin B1, ab16048, Abcam, 1:5000; anti-β-Actin, A1978, Sigma, 1:10,000) in 4% BSA/TBS + 0.025% sodium azide ...
-
bioRxiv - Cell Biology 2021Quote: ... The sections were then stained overnight at 4°C in the respective primary antibodies (anti-Rhodopsin, MAB5316 Sigma-Aldrich; anti-M-Opsin, AB5405 Sigma-Aldrich; anti-S-Opsin, AB5407 Sigma-Aldrich; anti-Iba-1 ...
-
bioRxiv - Cell Biology 2022Quote: ... Tissue sections were incubated with primary antibodies at 4° C overnight and then washed three times with PBS containing 0.2% Triton X-100 (PBSTx; Sigma-Aldrich, St. Louis, MO). The following antibodies were used ...
-
bioRxiv - Neuroscience 2019Quote: ... Sections incubated for 24 hours at 4°C in a primary antibody solution containing mouse anti-NeuN (catalogue no. MAB377; Millipore, Billerica, MA, USA) diluted 1:250 in 0.3% Triton-X and 3% normal donkey serum (NDS ...