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Citations for Millipore Sigma :
1551 - 1600 of 3147 citations for 6 ISOPROPYLINDOLE 97 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... and input TRF1 mutants were revealed with anti-His (anti 6-His Rabbit Pab Sigma Aldrich). For the detection of biotin-labelled PARylated proteins the same assay was conducted in presence of biotin-NAD+ (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies for cilia (mouse anti-acetylated tubulin, 1:1000 dilution, Sigma, cat# 6-11B-1) were diluted in block buffer and incubated on slides for 2hr at RT or overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... pooled (n=6) and homogenized in Tris-SDS lysis buffer with protease inhibitor (Sigma-Aldrich, USA). 10% homogenates were assayed for total protein content by Bradford method (1976) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were treated for 6 h with the appropriate concentration of either poly(I:C) (Sigma, P1530) or High Molecular Weight (HMW ...
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bioRxiv - Developmental Biology 2022Quote: ... and mouse anti-acetylated α-tubulin (clone 6-11B-1, Millipore-446 Sigma, #MABT868, 1:500) were diluted in 5% normal goat serum (NGS ...
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bioRxiv - Developmental Biology 2022Quote: ... and mouse anti-acetylated α-tubulin (clone 6-11B-1, Millipore-446 Sigma, #MABT868, 1:500) were diluted in 5% normal goat serum (NGS ...
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bioRxiv - Neuroscience 2020Quote: ... and the AMPA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 μM, Sigma Aldrich), for 2 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... cells were kept for 6 days in medium containing 1.25% DMSO (cell-culture grade; Sigma-Aldrich), and the differentiation status was validated using flow cytometry (CD11b ...
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bioRxiv - Immunology 2021Quote: ... 3 μg pRSV-Rev and 6 μg of pRRL lentiCRISPR vectors using Poly(ethyleneimine) (PEI; Sigma). Media was replaced 24 hours post-transfection and harvested 24 hours later for filtration with a 0.45 μm filter (SteriFlip ...
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bioRxiv - Developmental Biology 2019Quote: ... This was followed by incubation with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich; 1:10000) for 10 mins at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... Nuclei were digested for 6 minutes with 0.25 U MNase/mL (Sigma-Aldrich cat #N3755-500UN) and supplemented with 1.5 mM CaCl2 ...
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bioRxiv - Immunology 2019Quote: ... the plates were incubated with 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (A3219; Sigma-Aldrich) at room temperature for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... After washing off secondary antibodies 4′,6 diamidino-2-phenylindole (DAPI) (10μg/ml in PBS, Sigma) was used to visualize the nuclei ...
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bioRxiv - Cell Biology 2019Quote: ... The nuclei were counter-stained with 1 μg/ml DAPI (4′,6-diamidino-2-phenylindole, Sigma) for 5 minutes ...
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Genomic organization of the autonomous regulatory domain of eyeless locus in Drosophila melanogasterbioRxiv - Genetics 2021Quote: ... and finally stained for 4-6 h in lacZ staining solution with 0.2% X-gal (Sigma). The stained embryos were imaged in Leica stereomicroscope ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 6 times with washing buffer (1× PBS with 0.05% Tween-20 (Sigma-Aldrich)) and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma) ...
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bioRxiv - Systems Biology 2020Quote: ... aliquots of calcein NVOC (6 M in DMSO) were diluted 1:100 in PEG200 (Sigma-Aldrich), then in PBS to a final concentration of 1:5,000 for photoactivation of organoids and tissue sections ...
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bioRxiv - Cell Biology 2021Quote: ... AEP SS1 Hydra were relaxed for 1 min in 2% urethane (Sigma, Cat# 51-79-6)/Hydra medium ...
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bioRxiv - Bioengineering 2020Quote: ... and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich (St. Louis, MO, USA). NHS-rhodamine ...
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bioRxiv - Bioengineering 2020Quote: ... 2-deoxy-D-glucose (n=6; 60 mg, D8375-1G, Sigma-Aldrich, St. Louis, MO, USA), or saline (n=4 ...
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bioRxiv - Biochemistry 2020Quote: ... The Trx-His(6) tag was cleaved by addition of 300 units of thrombin (Sigma T4648) directly to the eluate and the solution dialyzed (Slide-A-Lyzer dialysis cassettes ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was extracted from 6 embryos using the Extract-N-Amp Tissue PCR Kit (Millipore-Sigma) and amplified using Amplitaq Gold Taq Polymerase (ThermoFisher) ...
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bioRxiv - Neuroscience 2020Quote: ... counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10000; D9542, Sigma-Aldrich, St Louis, USA), and mounted on glass slides with Mowiol 4-88 (Merck Millipore ...
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bioRxiv - Physiology 2021Quote: ... and plated in 6-well plates (0.25×106 cells per well) in William’s Medium E (Sigma) supplemented with antibiotics and 5% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... 7.5μM trichostatin A (TSA) 20μM 6-Chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide (SIRT1i) (EMD Millipore), 15μM SAHM1 (EMD Millipore) ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 6 times with washing buffer (1× PBS with 0.05% Tween-20 (Sigma- Aldrich)) and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma) ...
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bioRxiv - Pathology 2021Quote: ... Nuclei were visualized by incubating cells with DAPI (4′,6-Diamidine-2′-phenylindole dihydrochloride, Sigma Aldrich) at 0.2 mg/mL for 5 minutes at 20 °C ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich, St. Louis, MO, USA). Nine fields per well were imaged ...
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bioRxiv - Microbiology 2021Quote: ... the LAB samples were stained with the 6-carboxyfluorescein diacetate (CFDA, 10 μM, Sigma, Darmstadt, Germany). When the Caco-2 cells covered 80% of the area of the petri dish bottom ...
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bioRxiv - Cell Biology 2021Quote: ... Embryos were incubated with acetylated tubulin antibody (clone 6-11B-1, MABT868, Sigma-Aldrich, 1:1000) overnight at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Nuclear DNA was stained with DAPI (4’,6-diamidino-2-phenylindole) (1 mg/ml) (Sigma-Aldrich).
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bioRxiv - Cell Biology 2020Quote: The following antibodies were obtained commercially: anti-acetylated α-tubulin (clone 6-11B-1, Sigma T6793); anti-ARL13B (Proteintech 17711-1-AP) ...
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bioRxiv - Molecular Biology 2022Quote: ... fluorescein-labelled lectin peanut agglutinin (FITC-PNA) and 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich). Secondary antibodies for immunofluorescence and Western blot analysis were conjugated to Alexa 568 and Alexa 488 (Molecular Probes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were seeded into 6-well plates and permeabilized with digitonin (30 μg/mL; Sigma-Aldrich), followed by the collection of supernatant and cell lysate for further analyses as described previously (22) ...
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bioRxiv - Immunology 2022Quote: ... The slides were then stained with 1/1000 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) for 10 minutes at room temperature and washed twice for 5 minutes in PBS ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed 6 times with washing buffer (1× PBS with 0.05% Tween-20 (Sigma-Aldrich)) and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed 6 times with washing buffer (1× PBS with 0.05% Tween-20 (Sigma-Aldrich)) and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293T cells were seeded in a 6-well plate pre-coated with Poly-L-Lysine (Sigma) and transfected with expression plasmids ...
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bioRxiv - Cell Biology 2022Quote: ... fixed cells were stained with 0.1 μg/mL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI, Sigma). Coverslips were mounted in ProLong Gold Antifade (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 h at RT and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for 1 min ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1h and subsequently developed using 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) substrate (Sigma). Absorbance was measured at 405nm in a BioTek Synergy HTX multi-mode reader ...
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bioRxiv - Cancer Biology 2021Quote: The 6-well plates were coated with 12 mg/mL poly-hydroxyethylmethacrylate (polyHEMA, Sigma-Aldrich, P3932) in 95% ethanol ...
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bioRxiv - Biophysics 2020Quote: 6) Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA, Sigma, E3889-10G)
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bioRxiv - Molecular Biology 2020Quote: ... Nuclear DNA was stained with 100 µg/ml 4’,6-Diamidino-2-phenylindole (DAPI; Sigma #D9542). Images were acquired in an Opera High-Content Screening System with an APO 20x ...
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bioRxiv - Neuroscience 2021Quote: ... Cell nuclei were counterstained with 10 μM 4“,6-diamidino-2-phenylindole dihydrochloride (DAPI, Sigma-Aldrich) in dPBS++ ...
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bioRxiv - Cell Biology 2021Quote: Beta-TC-6 cells lysates were isolated by treating cell pellets with RIPA buffer (Sigma-Aldrich) supplemented with 1x protease and 1x phosphatase inhibitors (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... RE-cells were plated (750 cells/cm2) in 1.2%-polyhema-coated 6-wells (Sigma-Aldrich,USA). Mammosphere growth medium described in [47] ...
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bioRxiv - Neuroscience 2021Quote: ... PBS containing 50 ng/ml 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich, cat#D8417) was used for counter nuclear staining.
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bioRxiv - Biophysics 2021Quote: The FRET oligonucleotide 6-FAM-143D-TAMRA was obtained in HPLC-purified form from Sigma-Aldrich, St ...
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bioRxiv - Bioengineering 2020Quote: ... 6 hours after treatment the cells were lysed using 0.1% Triton X-100 in PBS (Sigma) and mixed with D-Luciferin assay (Promega ...