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Citations for Millipore Sigma :
1501 - 1550 of 10000+ citations for Recombinant Zebrafish IL 1 beta Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Mitochondrial ROS1 increases mitochondrial fission and respiration in oral squamous cancer carcinomabioRxiv - Cancer Biology 2020Quote: ... Anti-GAPDH (#G8795, 1:5000 for immunoblotting) and anti-myc tag (#05-724MG, 1:1000 for immunoblotting) antibodies were purchased from Sigma-Aldrich (St Louis, MO, USA). Anti-TOM20 (#sc-11415 ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies used for Western blot analysis include anti-beta-actin (Sigma-Aldrich, A2228) (1:10000) ...
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bioRxiv - Cancer Biology 2020Quote: ... so animals were administered with 8 μg/ml 17-beta estradiol (Sigma-Aldrich, #E2758) in drinking water at least 4 days previously to implantation until the end of the experiment ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.1 mM beta-mercaptoethanol and 106 units/L leukaemia inhibitory factor (LIF) (ESGRO, Millipore) and grown on a six well plate until 70% confluent ...
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Metformin protects trabecular meshwork against oxidative injury via activating integrin/ROCK signalsbioRxiv - Pharmacology and Toxicology 2022Quote: ... For drug testing, tBHP (Damas-beta, China) and metformin (Sigma-Aldrich, St. Louis, USA) were dissolved in DMEM/F12 ...
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bioRxiv - Biochemistry 2023Quote: The following antibodies were used for Western Blot: anti-Beta actin (Sigma-Aldrich, A5316), anti-Histone H3 (Cell signaling ...
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bioRxiv - Biophysics 2023Quote: ... cells were treated with 10 mM methyl-beta cyclodextrin (MbCD; Sigma-Aldrich, Darmstadt, Germany) in serum-free DMEM after three washing steps with DPBS+/+ to obtain a control sample featuring an increase in membrane fluidity ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfected Kuramochi control cells were then treated with TGF-beta (10 ng/ml; Sigma) for 2 hours before measurement to serve as a positive control for activated NF-κB activity ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 5 mg/L of phytosterols (Beta- sitosterol, Sigma, St-Louis, US, MO) and a final pH of 3.3 ...
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bioRxiv - Genetics 2024Quote: ... 0.1% beta-mercaptoethanol and 0.01% leukaemia inhibitory factor (mESC medium; Millipore, Massachusetts, United States). Cells were grown in petri dishes as semi-adherent colonies at 37°C with 5% CO2.
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bioRxiv - Cell Biology 2020Quote: ... the cell medium was replaced with DMEM containing 0.2% FBS and 300ng/mL IGF-1 (human recombinant, Sigma) for 3 hours ...
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bioRxiv - Biochemistry 2022Quote: ... and ∼1 μg recombinant GST or GST-SMN in freshly made 25 mM bis tris propane (Sigma B6755) pH 6.8 buffer with 1 M NaCl and 0.05% NP-40 ...
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bioRxiv - Neuroscience 2020Quote: ... dissolved in DMSO was injected in the otic cavity of 96 hpf zebrafish embryos mounted laterally in low gelling agarose (Sigma). The injected embryos were removed from the gel using E3 buffer and imaged within 1 hour of injections ...
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bioRxiv - Developmental Biology 2022Quote: ... adult DN mutant and Ctrl sibling zebrafish were bathed for 24 h in the presence of 5 μM 4-HT (H7904; Sigma) made from a 10 mM stock solution dissolved in 100% ethanol at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... 3 days post-fertilization (dpf) zebrafish larvae were anaesthetized for 2 - 3 minutes in embryo medium E3 containing 0.2 mg/ml Tricaine (Sigma-Aldrich). Thereafter ...
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bioRxiv - Neuroscience 2021Quote: Adult mosaic zebrafish (F0) were anesthetized in pH-buffered 0.2 mg/ml ethyl 3-aminobenzoate methanesulfonate solution (MS-222, Sigma-Aldrich). The caudal fin (2 mm of the end ...
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: Human endothelial cells or 3 days post-fertilization (dpf) zebrafish embryos with the yolk sac removed were lysed in RIPA buffer (Millipore) containing protease inhibitors (Roche ...
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bioRxiv - Microbiology 2020Quote: ... 5 days post fertilization (dpf) zebrafish larvae were anaesthetized with 0.017% Ethyl 3-aminobenzoate methanesulfonate (MS-222, Tricaine, Sigma-Aldrich) in egg water ...
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bioRxiv - Cell Biology 2024Quote: Age and sex matched 9-month-old zebrafish were sacrificed in 1g/l of MS-222 (Sigma Aldrich, MO, USA), fixed in 4% paraformaldehyde in PBS for 72 hour at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Larvae were anaesthetized in zebrafish water supplemented with 200 μg/ml tricaine (Ethyl 3-aminobenzoate methanesulfonate, MS-222 Sigma #A5040) before any manipulation (infection or imaging ...
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bioRxiv - Cell Biology 2023Quote: Adult zebrafish at the appropriate age were deeply anesthetized with 0.01% ethyl 3-aminobenzoate methane sulfonate (Tricaine, MS-222, Sigma Aldrich) and sacrificed by decapitation ...
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bioRxiv - Developmental Biology 2024Quote: For body length and brightfield images of lateral view head the zebrafish were first anesthetized in 0.016% Tricaine and transferred to 10% methylcellulose (Sigma, M7027), allowing correct positioning of the zebrafish during imaging ...
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bioRxiv - Developmental Biology 2024Quote: 24 hpf zebrafish embryos were treated with 20mM of the inhibitor 2,3-Butanedione 2-monoxime (BDM) (Sigma-Aldrich, Cat. #: B0753). Translation ribosomal affinity purification (TRAP ...
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bioRxiv - Neuroscience 2024Quote: Adult mosaic zebrafish (F0) were anesthetized in pH-buffered ethyl 3-aminobenzoate methanesulfonate solution (0.2 mg/ml; MS-222, Sigma-Aldrich). A section of the caudal fin was removed using dissecting scissors (WPI Inc. ...
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bioRxiv - Neuroscience 2021Quote: ... IL-4 (20 ng/mL, Sigma, 11276905001) or PBS ...
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bioRxiv - Immunology 2022Quote: ... and 10U/mL IL-2 (11147528001, Sigma).
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bioRxiv - Neuroscience 2023Quote: ... IL-1α (3 ng/mL, Sigma I3901) and TNF (30 ng/mL ...
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bioRxiv - Biophysics 2024Quote: IL-2 (Sigma Aldrich Cat #: I0523-5X20UG) was added to a final concentration of 1.25 µg/mL on Day 2 ...
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bioRxiv - Immunology 2024Quote: ... and IL-13 10ng/ml (Sigma-Aldrich) were isolated ...
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bioRxiv - Genetics 2023Quote: ... and IL-6 (#RAB0309, Sigma Aldrich, GmbH) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... 5 ng/ml IL-4 (Sigma I1020), and 20 µg/ml LPS (Sigma L-2630) ...
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bioRxiv - Developmental Biology 2024Quote: ... 100 ng/mL IL-3 (#I1646, Sigma) and 100 ng/mL Flt3 ligand (#F3422 ...
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Evidence for Involvement of WDPCP Gene in Alcohol Consumption, Lipid Metabolism, and Liver CirrhosisbioRxiv - Genetics 2023Quote: ... were incubated together with either 35 µL of PBST (for free glycerol measurements) or 35 µL of TAG reagent (Sigma, no. T2449, for TAG measurements) at 37 °C for 60 min ...
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bioRxiv - Developmental Biology 2022Quote: ... The IL-6 inhibitors LMT-28 and the Wnt/β-catenin inhibitor IWR-1 (I0161, SIGMA-ALDRICH) were both used at 10 μM.
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bioRxiv - Immunology 2022Quote: ... and then lysed for 15 min with a M-PER Mammalian protein extraction reagent (Pierce Biotechnology, Rockford, IL) containing a protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO) and 1 mM p-amidinophenyl methanesulfonylfluoride (PMSF) ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were mixed with 200uL of pre-washed slurry of a 1:1 mix of EZview Red Protein G and EZview Red Protein A bead affinity gel slurry (Sigma-Aldrich) and rotated at 4C for 1hr ...
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bioRxiv - Cancer Biology 2024Quote: ... and then incubated with protein A and protein G dynabeads (1:1 mix) and indicated antibody (H3K27ac, Diagenode, #C15410196; TFE3, Sigma, #HPA023881) at 4c overnight ...
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bioRxiv - Cell Biology 2023Quote: ... protein inhibitor cocktail (1:100) and Benzonase nuclease (Novagen, 70746, 1:100) in PBS ...
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bioRxiv - Plant Biology 2021Quote: The inhibitory effect of recombinant P235 on recombinant glycosyl hydrolase was assayed using the β-Glucosidase Activity Assay Kit (MAK129, Sigma). Enzymatic reactions were carried out in K-Phosphate buffer (100 mM ...
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iDePP: a genetically encoded system for the inducible depletion of PI(4,5)P2 in Arabidopsis thalianabioRxiv - Plant Biology 2020Quote: ... 2 ml of 50 % HIS-Select® Cobalt affinity resin (Sigma) was washed with milli-Q water and then with equilibration buffer (20 mM imidazole ...
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 800 mM) ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 300 mM) ...
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bioRxiv - Microbiology 2021Quote: ... and a combination of α-6×His antibody (Sigma Aldrich, H1029) and HisProbe-HRP (Thermo ...
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bioRxiv - Cell Biology 2021Quote: His-ACBD5 was expressed in BL21 Rosetta (DE3) cells (EMD Millipore) induced with 1 mM IPTG overnight at 18°C ...
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bioRxiv - Microbiology 2021Quote: ... Ni2+-NTA His-bind slurry was obtained from Novagen (Darmstadt, Germany). Rhodamine B isothiocyanate-Dextran (RITC-Dextran ...
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bioRxiv - Cell Biology 2020Quote: ... followed by immunoblotting with HRP-conjugated anti-His antibody (Sigma-Aldrich) by using SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... or 15 U/mg His-tagged SUMO protease (Millipore Sigma SAE0067) by overnight incubation at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... –Ade (20 ug/mL L-His HCl monohydrate (A-9126, Sigma) added to –Trp ...
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bioRxiv - Immunology 2021Quote: ... either supplemented with 10% HI dialysed and filtered FCS (Sigma-Aldrich) 0.1M HEPES ...