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Citations for Millipore Sigma :
1351 - 1400 of 10000+ citations for Recombinant Zebrafish IL 1 beta Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Recombinant 2N4R human Tau (Tau441, AG960, Millipore) at serial dilutions ranging from 20 µg/ml to 0 µg/ml total tau was used to generate a calibration curve and calculate total tau in our samples.
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bioRxiv - Genetics 2022Quote: ... 10 μg/ml recombinant human insulin (Sigma), 2 IU/ml heparin (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... 10 ng/mL human recombinant bFGF (Sigma), and 10 μM Y-27632 (STEMCELL Technologies)) ...
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bioRxiv - Genomics 2023Quote: ... 10 μg/mL recombinant human insulin (Sigma), 2 IU/mL heparin (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.8μg of recombinant LSD1 (Sigma-Aldrich SRP0122) was used as positive control ...
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bioRxiv - Cell Biology 2024Quote: ... human recombinant Insulin solution 5µg/mL (Sigma), M-CSF 100ng/mL (Invitrogen ...
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bioRxiv - Immunology 2024Quote: Human recombinant insulin (Millipore Sigma, Cat#19278), GAD65 (Diamyd Medical ...
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bioRxiv - Microbiology 2024Quote: ... recombinant PDGFRα-Fc or heparin agarose (Sigma) overnight at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... 5 μL of the dye solution was added to 1 mL 5 mg/mL HI (91077C, Sigma-Aldrich, 95%) monomer solution ...
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bioRxiv - Biochemistry 2021Quote: ... and the lysate was incubated for 1 h with a previously equilibrated His-Select Nickel Affinity Gel (Sigma-Aldrich). Weakly-bound proteins were removed using buffer A (20 mM imidazole ...
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bioRxiv - Plant Biology 2022Quote: ... The transformants were then spotted on SD (−Trp, −Leu, −His) selection media containing 1 mM 3-amino-1,2,4-triazole (Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
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bioRxiv - Molecular Biology 2023Quote: ... The 8 ml supernatant was applied to 1 ml packed volume of Ni-NTA His-Bind Resin (Millipore Sigma). Binding was performed with gentle mixing for 1 hour at 4°C and loaded onto a Poly Prep Chromatography Column (BioRad) ...
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bioRxiv - Immunology 2023Quote: ... and digested in 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma ...
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bioRxiv - Cell Biology 2022Quote: Serum IL-6 levels were quantified using a commercial mouse IL-6 ELISA kit (RAB0308, Sigma) according to the manufacturer’s instructions and analyzed using spectra-iMAX (Molecular Devices).
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bioRxiv - Cell Biology 2024Quote: ... M0 macrophages were polarized to M2a macrophages using IL-4 and IL-13 (both Sigma-Aldrich), each 20 ng/ml ...
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bioRxiv - Neuroscience 2020Quote: ... EAE in bone marrow transplanted male animals was induced in anesthetized 8-10 week-old animals after irradiation by subcutaneous injection at the tail base of 10μg of recombinant rat MOG together with incomplete Freud’s adjuvant 1:1 (Sigma, St Louis, MO). EAE was induced in 10-12weeks old female animals using the same protocol using 10μg MOG ...
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bioRxiv - Immunology 2022Quote: ... and 10U/mL IL-2 (Sigma) in complete RPMI ...
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bioRxiv - Microbiology 2023Quote: ... or IL-1RA (SRP3327, Sigma-Aldrich). At the indicated time points ...
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bioRxiv - Neuroscience 2023Quote: ... Lithium chloride (LiCl; Sigma-Aldrich, IL) was dissolved in water to a concentration of 0.15M and administered intraperitoneally (10 mL/kg).
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bioRxiv - Molecular Biology 2022Quote: ... IL-4 (5 ng/ml; Sigma) 0.5 μg/ml of anti-CD180 (RP105 ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 ng/mL IL-4 (Sigma), and 0.5 ug/mL anti-CD180 (BD Pharmingen ...
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bioRxiv - Cell Biology 2023Quote: ... mouse IL-6 (Sigma-Aldrich, RAB0308), mouse TNF-α (Cayman ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... IL-1α (3 ng/ml; Sigma), and C1q (400 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... and IL-2 (Millipore, Burlington, MA). Differences in induction of proteins post stimulation were calculated using log10(pg/mL fold-change +1) ...
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bioRxiv - Cancer Biology 2021Quote: ... Western blots were performed using following antibodies: beta-actin (AC-15, Sigma-Aldrich), beta-actin (13E5 ...
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bioRxiv - Biophysics 2020Quote: ... or 2 ng/µl mouse anti-beta-tubulin primary antibody (Sigma-Aldrich, T8328), the clathrin samples were incubated with 2 ng/µl rabbit anti-clathrin primary antibody (Abcam ...
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bioRxiv - Biophysics 2021Quote: ... nigericin and methyl-beta-cyclodextrin were purchased from Sigma-Aldrich (St Quentin, France). Dulbecco’s PBS (DPBS ...
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bioRxiv - Neuroscience 2023Quote: ... cell pellets were resuspended in lysis buffer supplemented with beta-mercaptoethanol (Sigma, #63689) and homogenized using a handheld rotor with an attached pellet pestle for 30-45 seconds followed by a 2-minute high-speed centrifugation to remove insoluble cell debris.
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bioRxiv - Biophysics 2023Quote: The TwoMP instrument was calibrated with Beta-Amylase from Sweet Potato (Sigma A8781) and Thyroglobulin from Bovine Thyroid (Sigma T9145 ...
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bioRxiv - Cell Biology 2024Quote: ... the sEVs were incubated with 5 mM methyl-beta-cyclodextrin (MβCD) (Sigma‒Aldrich) overnight at room temperature with gentle shaking or incubated with 200 μM saponin (Kanto Chemical ...
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bioRxiv - Neuroscience 2024Quote: ... Beta-amyloid plaques were stained using Congo Red (15μM, #C6277, BioXtra, Sigma Aldrich) and the vasculature was immunostained with an anti-transgelin-targeting (SM22 ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 2.5% beta-mercaptoethanol (Sigma Aldrich, St. Louis, MO; Cat. No. M6250) and heat-denatured for 10 min at 95°C ...
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bioRxiv - Microbiology 2020Quote: ... was used for SDS-PAGE and Western blotting in Tris-buffered saline and 0.2% Tween 20 (TBST) using Strep-Tag II HRP (1:2,500; EMD Millipore) and rabbit anti-vinculin (1:1,000 ...
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bioRxiv - Microbiology 2022Quote: ... incubated 1 hour with primary mouse monoclonal antibody anti-HA tag (G036, Abm Inc.) diluted 1:10,000 and then probed with horseradish peroxidase-labeled anti-mouse immunoglobulin (A9044, Sigma), diluted 1:15,000 ...
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bioRxiv - Cell Biology 2022Quote: ... for Strep-tagII::MLC-4 detection or mouse anti-6xHistidine tag antibody (Millipore clone HIS.H8, 1:2,500, 05-949) for 6xHIS::NMY-2 detection ...
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bioRxiv - Microbiology 2024Quote: 50 nM of recombinant PL6 enzymes was incubated with 5 mg mL-1 brown seaweed alginate (Sigma) in 20 mM of varying buffers to determine pH optima at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... This was followed by 0.5ug/mL anti-his HRP (Sigma), and binding was detected after incubation with substrate at an absorbance of 450nm ...
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bioRxiv - Biochemistry 2020Quote: ... The antibodies used for western blotting were anti-His (Sigma), anti-Strep HRP conjugated (iba ...
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bioRxiv - Molecular Biology 2021Quote: ... SD-Leu-Trp-His+1mM 3-AT (3-aminotriazol, Sigma) and SD-Leu-Trp-His-Ade (Formedium).
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bioRxiv - Genetics 2021Quote: ... His-TCF7 was PCR cloned into pET52b (Millipore, 71554-3) using the primers 5’-CGGGGTACCTCATGCCGCAGCTGGACTCC-3’ and 5’-ATAGTTTAGCGGCCGCGAGCACTGTCATCGGAAGGAAC-3’ ...
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bioRxiv - Biochemistry 2021Quote: α-PCNA (P8825) and α-HIS (H1029) were purchased from Sigma; α-Cul2 (EPR3104 ...
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bioRxiv - Genetics 2022Quote: ... and detected with His and GST antibodies (Sigma-Aldrich, USA), respectively.
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bioRxiv - Molecular Biology 2024Quote: ... One milliliter of equilibrated HIS-Select Nickel Affinity Gel (Sigma) was added to the cleared cell lysate and incubated with mild agitation for 1 h at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... and BRAF NTs/ BRAF KD with His antibody (Sigma SAB5600227). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3% heat-inactivated fetal bovine serum (HI-FBS; Sigma) at 37°C for 15 ∼ 30 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... Anti-His primary antibody (H1029) was purchased from Sigma Aldrich, HRP conjugated secondary antibody (J1430 ...
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bioRxiv - Cancer Biology 2023Quote: ... which were cryopreserved in HI FBS with 10% DMSO (Sigma). Cells were directly transferred to a -80 °C freezer in Coolcell freezing containers (Corning ...
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bioRxiv - Biochemistry 2023Quote: ... 25 uL of (His)6-tagged TEV-protease (Sigma-Aldrich) was added and the solution incubated overnight at 4°C.
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% HI-horse serum and vancomycin (Sigma, USA). The inoculated flask was then kept in shaking conditions (100 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... 2% heat-inactivated Fetal Bovine Serum (HI-FBS; Sigma-Aldrich), 1% v/v penicillin-Streptomycin (VWR) ...