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Citations for Millipore Sigma :
1451 - 1500 of 2589 citations for Rat FAM24A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... expanded and the plasmid DNA was extracted (Sigma, PNL350). Lentiviruses were generated in HEK293T cells and then used to infect Cas9 cells ...
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bioRxiv - Biophysics 2022Quote: ... The plasmids were transformed into BL21(DE3) cells (Sigma) using heat-shock ...
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bioRxiv - Cancer Biology 2022Quote: ... the MISSION TRC2 pLKO.5-puro plasmid (Sigma-Aldrich) was modified to replace the puromycin-resistance gene with the enhanced green fluorescent protein (EGFP ...
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bioRxiv - Cell Biology 2020Quote: ... The 3xFlag sequence from p3xFLAG-CMV-7.1 plasmid (Sigma) was inserted downstream of the AID sequence ...
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bioRxiv - Cancer Biology 2019Quote: ... Control and knockdown ADAMDEC1 plasmids were purchased from Sigma. Different clones for each shRNA plasmid were tested and the best knockdowns were selected to produce lentiviral particles ...
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bioRxiv - Bioengineering 2019Quote: ... coli expression and cloned into a pET28a(+) plasmid (Novagen) with an N-terminal hexahistidine-tag and C-terminal GSGRRRRRRRR sequence ...
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bioRxiv - Microbiology 2020Quote: ... coli using a Sigma GenElute Plasmid Kit (Sigma Aldrich). Plasmids used in this study are shown in Table 4 ...
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bioRxiv - Cell Biology 2019Quote: ... The 3xFlag sequence from p3xFLAG-CMV-7.1 plasmid (Sigma) was inserted downstream of the AID sequence ...
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bioRxiv - Biophysics 2021Quote: ... Plasmids were transiently transfected using Genejuice (Novagen/EMD Millipore) into HEK293T cells ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid p3XFLAG -CMV-7.1 was obtained from Sigma (E7533).
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bioRxiv - Microbiology 2023Quote: ... PCR products and the pET17b plasmid (Novagen (EMD Millipore), cat ...
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bioRxiv - Microbiology 2023Quote: ... PCR products and the pET17b plasmid (Novagen (EMD Millipore), cat ...
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bioRxiv - Neuroscience 2022Quote: ... and the plasmids mixed with Fast Green dye (Sigma) were microinjected into one of the lateral ventricles for CPN circuit analysis and axon GC isolation experiments ...
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bioRxiv - Genomics 2023Quote: ... The plasmid library was prepared by maxi-prep (Sigma), digested with SphI-HF and SpeI-HF (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... and subcloned into a modified pCDF-1b plasmid (Novagen) resulting in expression of MsbA with an N-terminal TEV protease cleavable His6 fusion protein ...
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bioRxiv - Biophysics 2023Quote: ... UBQLN2-ΔUBA and ubiquitin (Ub) in pET24b (+) plasmid (Novagen) were grown in Luria-Bertani (LB ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmids were transformed into E.coli Rosetta2 (DE3) cells (Novagen). Bacteria were grown in LB medium at 37 °C up to an OD600nm of 0.6 ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmid DNA constructs were cloned into pET-24b (Novagen) expression vector by site-directed mutagenesis and the coding sequences of all mutants were confirmed by DNA sequencing prior to use.
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bioRxiv - Cancer Biology 2022Quote: ... Plasmids were separately transfected into BL21-DE3-Rosetta (Novagen). Proteins were expressed overnight at 18°C in TB medium (Melford ...
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bioRxiv - Immunology 2023Quote: ... CAS9 Blasticidin Lenti plasmid was purchased from Sigma (CAS9BST). Plasmids were expanded in house and isolated using Plasmid Plus Midi Kit (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... into the pTriEx™-1.1 Hygro plasmid (Novagen 70928). Stably expressing cell lines were generated using FuGENE® HD Transfection Reagent (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... when needed for plasmid maintenance or selection (Sigma-Aldrich). Escherichia coli was grown at 37°C in LB medium with 100 μg/ml ampicillin and/or 20 μg/ml chloramphenicol (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... using the GenElute™ HP plasmid MidiPrep kit (Sigma). Plasmids for expression of ERp44wt and C29S mutant were a kind gift of Roberto Sitia.
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bioRxiv - Biochemistry 2024Quote: ... The plasmid was transformed into Rosetta2(DE3) cells (Novagen) and grown in 1L Terrific Broth (TB ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmid was transformed into Rosetta2(DE3) cells (Novagen) and grown in 1 L TB media with 100 µg/ml ampicillin and 0.05 mM D-(+)-biotin for in vivo biotinylation ...
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bioRxiv - Biophysics 2020Quote: ... cells were plated sparsely on glass coverslips coated with rat-tail collagen I (Cat # 3867, Sigma) at a coating density of 10 µg/cm2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Male Wistar rats were intravenously (IV) injected with puromycin aminonucleoside (PAN) (Sigma-Aldrich, St. Louis, MO) and treatment groups were treated by oral gavage with Pioglitazone (Alfa Aesar ...
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bioRxiv - Immunology 2021Quote: ... Rat splenocytes (2 × 105) were plated in duplicate in 96-well PVDF plates (Millipore, Billerica, MA) and stimulated with 5 μg/ml of recombinant 2019-nCoV Spike protein (S1+S2 ECD ...
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bioRxiv - Immunology 2022Quote: Serum insulin was determined using a rat/mouse insulin ELISA kit (Millipore, Billerica, Merck KGaA, USA) as instructed ...
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bioRxiv - Cell Biology 2019Quote: ... Blots were labelled with the appropriate set of antibodies: primary rat anti-HA (1:500, Sigma), mouse anti-Myc (1:1000 ...
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bioRxiv - Plant Biology 2019Quote: ... and then with a goat anti-rat IgG HRP-conjugated secondary antibody (AP202P, 1:10,000, Sigma). HRP activity was detected by the Supersignal Western Detection Reagents (Thermo Scientific).
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bioRxiv - Neuroscience 2019Quote: ... rats were pretreated with atropine methylbromide (5 mg/kg, i.p.; Sigma-Aldrich, Inc.; St. Louis, MO) 20 min before seizure induction with pilocarpine hydrochloride (345 mg/kg ...
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bioRxiv - Synthetic Biology 2019Quote: ... Supernatants and lysates were separated by SDS-PAGE followed by immunoblotting with rat anti-HA (Sigma) antibody to evaluate the presence of recombinant CD47nb protein in culture fractions ...
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bioRxiv - Genetics 2021Quote: ... Mouse plasma insulin and leptin levels were determined by using Rat/ Mouse Insulin ELISA Kit (Millipore, EZRMI-13K for insulin and EZML-82K for leptin ...
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bioRxiv - Developmental Biology 2021Quote: ... the uterus was excised from the mated female rat and flushed with M2 medium (M7167, Sigma). The flushed blastocyst-cell stage embryos were selected under a dissecting microscope and rechecked by an inverted microscope (BM ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used were against β-III tubulin (Tuj-1; mouse anti-rat, 1:300; Millipore), choline acetyltransferase (ChAT ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were used at the following concentrations: rat anti-HA 3F10 at 1:400 (Sigma-Aldrich), mouse anti-Myc 9B11 at 1:2000-1:3000 (Cell Signaling Technology ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was then allowed to bind to affinity-purified rat anti-HA antibody (Sigma) attached to paramagnetic beads (Dynabeads protein G ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with primary antibody rat anti-somatostatin (1:250, Millipore cat. No. MAB354, RRID:AB_2255365) at RT for 24 hours and then 4°C for 42 hours ...
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bioRxiv - Microbiology 2020Quote: ... and probed with 1:1000 diluted primary anti-Ago2 rat monoclonal antibody clone 11A9 (Millipore Sigma/ Merck KGaA MABE 253 ...
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bioRxiv - Bioengineering 2020Quote: ... P0-1 SD rat brains were removed and rinsed in phosphate buffer saline (PBS, Sigma-Aldrich). After careful removal of the meninges and brains cortices ...
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bioRxiv - Bioengineering 2022Quote: ... membranes were coated with a mixture of rat tail type I collagen (25 µg/cm2, Sigma) and fibronectin (5 µg/cm2 ...
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bioRxiv - Cell Biology 2022Quote: ... The rabbit-derived secondary antibody (anti-rat) conjugated with 10-nm colloidal gold (Sigma, lot #SLBZ8963), the goat-derived secondary antibody (anti-mouse ...
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bioRxiv - Cell Biology 2022Quote: ... Collagen type I solution from rat tail (C3867-1VL) and collagenase (C9263) were purchased from Sigma–Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Anti-rat PLA plus and minus probes were made using the PLA probemaker kit (Sigma Aldrich) with a Goat anti-rat IgG antibody (Jackson Immunoresearch ...
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bioRxiv - Physiology 2020Quote: ... Insulin release from islets was measured as described with Rat/Mouse Insulin ELISA kit (Millipore Sigma) (26).
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bioRxiv - Neuroscience 2021Quote: Mouse neuroblastoma x rat neuron hybrid ND7/23 cells were purchased from Sigma-Aldrich (ECACC 92090903). They were grown in high-glucose Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Neuroscience 2020Quote: ... Rats were initially trained to lick for sodium saccharin solution (0.2% in distilled water; Sigma 47839) during 60 min sessions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Orphan GPCR expression was detected using rat anti-HA tag (clone 3F10) antibodies (Sigma-Aldrich; 11867423001) or rabbit anti-myc tag antibodies (GenScript ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... A single dose (100µl in rats, 20µl in mice) of complete Freund’s adjuvant (CFA, SIGMA Aldrich) was injected subcutaneously into the plantar surface of one hind paw using a microliter syringe with a 27-gauge needle ...