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Citations for Millipore Sigma :
1351 - 1400 of 2589 citations for Rat FAM24A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: Olfactory bulb sections containing rat glomeruli were treated with glycine (50 mM, Sigma-Aldrich) and ammonium chloride (50 mM ...
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bioRxiv - Genomics 2023Quote: ... The stainings were quenched with the addition of 50 ul Rat Serum (Sigma, R9759) and incubation at 4°C for 10 minutes protected from light ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with the primary antibody anti-ATE1 (1:200, rat, Sigma-Aldrich), anti- calreticulin (CALR ...
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bioRxiv - Biochemistry 2023Quote: ... TR from rat liver (TrxB) was obtained from Sigma-Aldrich (St. Louis, MO, USA). pGEX-4T-1 cells were obtained from GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... as in28 and rat anti-laminin-2 1:100 233 (Sigma-Aldrich, Cat. #L0663). For macrophage staining these included ...
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bioRxiv - Genetics 2023Quote: ... and rat monoclonal anti-laminin-2 (α2 chain; 4H8-2; Sigma Aldrich; 1:500). Secondary antibodies used were goat polyclonal anti-rabbit Alexa Fluor 594-conjugated (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: The rat cardiomyoblast cell-line H9c2 (cells derived from embryonic heart tissue; Sigma Aldrich) was genetically modified using retrovirus to achieve a stable expression of tandem tagged (mCherry-EGFP ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies were used at the following dilutions: Rat anti-HA IgG1 (clone 3F10; Sigma) at 1:2000 ...
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bioRxiv - Physiology 2023Quote: The following antibodies were used: rat anti-laminin-α2 (1:1500, Sigma-Aldrich, L0663), mouse anti-BA-D5 (MyHC-I ...
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bioRxiv - Molecular Biology 2022Quote: ... in a 10-cm dish transfected one day post seeding with packaging plasmids (1 μg VSV.G, 2 μg psPAX2 and 4 μg of transfer plasmid using PEI (Sigma-Aldrich). Virus containing supernatant was collected 72 hours post transfection ...
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Diversified expression of gamma-protocadherins regulates synaptic specificity in the mouse neocortexbioRxiv - Neuroscience 2021Quote: ... The plasmid mixture with the total concentration of 5mg/ml plasmid (equal molar mixture if there are multiple plasmids) containing 1mg/ml Fast green (Sigma) was injected into the embryonic lateral ventricle through a glass pipette ...
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bioRxiv - Bioengineering 2021Quote: ... DNA oligonucleotides and the GenElute HP Plasmid Miniprep and Midiprep kit used for plasmid purifications were acquired from Sigma-Aldrich. Q5 High-Fidelity DNA Polymerase ...
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bioRxiv - Biophysics 2021Quote: ... Cells were harvested by centrifugation at 6000xg for 10min and the pellet was re-suspended in LB/kanamycin for plasmid extraction and purification using a GenElute plasmid miniprep kit (Sigma).
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bioRxiv - Biochemistry 2021Quote: ... we used a dual-luciferase reporting system based on a plasmid containing the sequence for Renilla luciferase and the multiple cloning site (MCS) from the plasmid pMLuc-1 (Novagen) upstream of the firefly luciferase sequence in the plasmid pISO (addgene) ...
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bioRxiv - Plant Biology 2022Quote: ... coli cells that contained pL4440-CP were grown in LB supplemented with carbenicillin (100 μg/mL) followed by plasmid DNA extraction with the High Pure Plasmid Isolation Kit (Sigma). For the in vitro synthesis ...
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bioRxiv - Genomics 2024Quote: ... and plasmid DNA was purified from the resulting cell pellet using a GenElute HP Plasmid Maxiprep Kit (Sigma-Aldrich #NA0310) following the manufacturer’s recommended protocol with elution in 3 mL H2O ...
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bioRxiv - Molecular Biology 2023Quote: ... and was mixed with 1 µg total DNA (100 ng receptor plasmid + 200 ng mGs plasmid + 700 ng salmon sperm DNA (Sigma) solubilized in 100 µL OptiMEM ...
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bioRxiv - Microbiology 2023Quote: ... Bacteriophage DNA was isolated using the Phage DNA Isolation Kit (Norgen) and plasmids were isolated with the GenEluteTM Plasmid Miniprep Kit (Sigma). DNA purification from agarose gel or enzymatic reactions was performed with the NucleoSpin Gel and PCR clean up kit (Macherey-Nagel) ...
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bioRxiv - Microbiology 2024Quote: All plasmids that were generated in this work are based on the backbones of plasmid pWL102 (DSM 5717) or pET15b (Novagen). The full list of generated plasmids and the oligonucleotides used in this work are summarized in Table S2 and S3 ...
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bioRxiv - Microbiology 2024Quote: ... The plasmid containing the desired complement was extracted from the confirmed colonies using GenElute™ Plasmid Miniprep Kit (Sigma-Aldrich). The plasmid containing the required complement was then electroporated into the phage-resistant mutants ...
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bioRxiv - Genetics 2024Quote: ... and 3-5 µl plasmid solution (Plasmid concentration 100-200 ng/µl) containing Phenol Red for visualization (0.1% Sigma #P0290) was injected intramuscularly using a Nanofil syringe (WPI ...
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bioRxiv - Plant Biology 2020Quote: ... using the GenElute Plasmid Miniprep Kit (Sigma-Aldrich). Inserts were sequenced (GATC Services ...
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bioRxiv - Biophysics 2021Quote: ... coli expression plasmid vector pET-28b (Novagen, MA) was used to construct all the gp17 WT and mutant clones (37) ...
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bioRxiv - Biochemistry 2019Quote: ... of the plasmid and BacVector3000 baculovirus DNA (Novagen) using the calcium phosphate co-precipitation method ...
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bioRxiv - Microbiology 2019Quote: Expression plasmids were transformed into BL21(DE3) (Novagen) and selected overnight at 37°C on Luria-Bertani (LB ...
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bioRxiv - Microbiology 2022Quote: ... The plasmid-pET28a was purchased from Novagen (Germany). Ni-NTA agarose was purchased from G-Biosciences (USA) ...
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bioRxiv - Developmental Biology 2019Quote: ... Antigens were made using a pET28b (+) plasmid (Novagen) for expression of 6XHis tagged proteins ...
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bioRxiv - Biochemistry 2019Quote: ... all expression plasmids were created in pET21a (Novagen; N-terminal T7•tag® and C-terminal His•tag® ...
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bioRxiv - Biochemistry 2021Quote: ... in conjunction with a pET-21A plasmid (Novagen) containing WT Cre (provided by Gregory Van Duyne ...
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bioRxiv - Biophysics 2021Quote: ... Plasmids were transformed into Rosetta(DE3) pLysS (Novagen) E ...
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bioRxiv - Microbiology 2022Quote: ... and inserted into the expression plasmid pET30a (Novagen). Recombinant SIRV2 gp21 and SiL_0190 with C-terminal His-tag were expressed using Escherichia coli RosettaTM (DE3 ...
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bioRxiv - Biochemistry 2024Quote: Recombinant plasmids were transformed into Tuner cells (Novagen) in Luria-Bertani (LB ...
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bioRxiv - Biochemistry 2024Quote: ... into the pET-28a expression plasmid (EMD Millipore). Protein was expressed and purified as described above ...
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bioRxiv - Biochemistry 2024Quote: ... and then cloned into a pET28 plasmid (Novagen) with T4 DNA ligase (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... Expression plasmids based on the pET28a backbone (Novagen) were constructed by Gibson Assembly75 ...
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bioRxiv - Neuroscience 2024Quote: ... The plasmid solution with Fast Green (Sigma-Aldrich) was injected into the lateral ventricle of the mouse embryos and electroporated (37–40V ...
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bioRxiv - Genetics 2024Quote: ... or GenEluteTM HP Plasmid Midiprep kit (Sigma-Aldrich) or PureLinkTM HiPure Plasmid filter Maxiprep Kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... gRNA expressing plasmids were purchased from Sigma Aldrich (U6-gRNA:hPGK-puro-2A-tBFP ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary and secondary antibodies were used: rat anti-SST (1:200, Millipore #MAB354), chicken anti-PV (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Antibodies were used at the following dilutions: rat anti-HA epitope IgG1 (clone 3F10; Sigma) at 1:5,000 ...
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bioRxiv - Physiology 2019Quote: ... RT-qPCR was carried out as previously described [20] using rat-specific oligonucleotide primers (Sigma) or FAM-MGB Taqman probes (see Supp Table 1 for primer list) ...
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bioRxiv - Cell Biology 2019Quote: ... followed by incubation with mouse anti-rat GFP and CD90 antibodies (1:100; Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Physiology 2020Quote: ... Afterwards the rats were anaesthetized with urethane (1.5 g/kg b.w. i.p., Sigma-Aldrich, Poland) and were subjected to hemodynamic measurements including ABP and LVP recordings as described above for SHHF and SD rats.
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... rats received intra-cerebral saline or d-amphetamine (10 or 30 µg/hemisphere; Sigma-Aldrich, Dorset ...
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bioRxiv - Neuroscience 2020Quote: Serotonin immunolabeling was performed with 1:25 rat anti-serotonin (Millipore Sigma, Cat#MAB352, RRID:AB_11213564), 1:1000 rabbit anti-serotonin (ImmunoStar ...
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bioRxiv - Plant Biology 2020Quote: ... and hybridized with peroxidase conjugated goat anti-rat (Polyclonal, A9037, Sigma, www.sigmaaldrich.com; 1:5000 dilution) and after membrane stripping ...
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bioRxiv - Pathology 2021Quote: ... tissues were stained overnight at 4°C with rat anti-laminin (1:1000, Sigma L0663) in blocking buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were seeded on plates coated with 50 µm/ml rat tail collagen I (Sigma).
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bioRxiv - Synthetic Biology 2022Quote: ... Nonspecific antibody binding was blocked by incubation with 20 µg/ml Rat IgG (Sigma, I4131) for 5 min ...
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bioRxiv - Plant Biology 2021Quote: ... After incubation with HRP-conjugated secondary goat antibody against rabbit or rat primary antibodies (Sigma), detection was performed with the Clarity Max Western ECL substrate (#1705062 ...