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Citations for Millipore Sigma :
1451 - 1500 of 1805 citations for FITC labeled Fibrinogen since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... gut permeability was evaluated by gavaging mice that were fasted for 4 h with 5 mg FITC-dextran 4000 (Sigma -Aldrich) in 200 μl PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Retinas were isolated at postnatal day 5 and stained with 10 μg/ml FITC-conjugated lectin from Griffonia simplicifolia (isolectin-B4)48 (L9381, Sigma, MO). Images were collected from each lobe and analyzed by an observer blinded to mouse genotype ...
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bioRxiv - Genetics 2023Quote: ... 6H4 and 4H8 (1:100 for each) [71] and mouse anti-α tubulin DM1A conjugated directly to FITC (Sigma, 1:50). The secondary antibodies that were used were Cy3 ...
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bioRxiv - Immunology 2022Quote: ... fibroblasts and MCF 10A cells were incubated with the FITC bound anti-mouse IgG secondary immunoglobulin (Fc specific) (Cat # F4143, Sigma-Aldrich) secondary antibody for 2 h at room temperature away from light ...
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bioRxiv - Cell Biology 2022Quote: ... Anti alpha tubulin-FITC mouse mAb (#F2168) and 4’,6-diamidine-20-phenylindole dihydrochloride (DAPI, #10236276001) were purchased from Sigma Aldrich, Germany ...
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bioRxiv - Immunology 2022Quote: ... Blood was collected via tail nick using a heparinized capillary tube prior to gavage (baseline or 0 minute sample) with 4 kDa fluorescein isothiocyanate-conjugated dextran (FITC-dextran; cat#46944, Sigma-Aldrich) diluted in PBS (pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... Smooth muscle cells were identified by immunostaining with fluorescein isothiocyanate (FITC)-conjugated primary antibody against α-smooth muscle actin (1:100; clone 1A4, Sigma), followed by anti-FITC biotin-conjugated secondary antibody (1:400 ...
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bioRxiv - Developmental Biology 2023Quote: Third instar larvae were fed on semi-solid drops of 500 kDa FITC-labelled dextran (Sigma-Aldrich, St. Louis, MO, USA), diluted to 1 mg/mL in sucrose-agar medium (5% sucrose mixed in 3% agar medium ...
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bioRxiv - Synthetic Biology 2023Quote: ... pH 7.2) at a concentration of 10 mg/mL and fluorescein isothiocyanate (FITC; Sigma-Aldrich, St. Louis, MO, cat. no. F7250) was added in a 25x molar excess from a 10 mg/mL solution in dimethylsulfoxide (DMSO) ...
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bioRxiv - Cancer Biology 2023Quote: C6 cells were treated with indicated nanoformulations for fixed time points followed by Annexin V/ propidium iodide (PI) staining using Annexin V-FITC Apoptosis Detection Kit II (Cat # PF032, Sigma-Aldrich), as per manufacturer protocol ...
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bioRxiv - Immunology 2024Quote: ... they were orally gavaged with fluorescein isothiocyanate (FITC)-conjugated 4 kDa dextran (50 mg per 100 g body weight) (46944, Sigma-Aldrich). Four h after gavage ...
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated with secondary antibody in PBS (1:250) anti-Mouse IgG (whole molecule) –fluorescein isothiocyanate (FITC) antibody produced in goat (Sigma, F9006) in dark for 1 h ...
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bioRxiv - Immunology 2024Quote: ... in OptiMEM medium supplemented with dyes conjugated with dextran molecules of defined sizes (Alexa647-10kDa dextran from ThermoFisher Scientific and FITC-500kDa from Sigma Aldrich). Acquisitions were performed under a Leica DMi8 microscope at 37°C with a 10x magnification objective (N.A ...
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bioRxiv - Microbiology 2024Quote: ... was added to the basal chamber and 50 µL of anaerobic HBSS with 10 µM 4 kDa FITC-Dextran (Sigma-Aldrich) was added to each apical chamber before incubation at 37 °C for 1 h ...
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bioRxiv - Immunology 2024Quote: ... Sections were incubated overnight with antibodies targeting α−smooth muscle actin (SMA) conjugated to FITC (Sigma Aldrich Clone 1A4, Lot 0000196944), S100A8 (R&D systems Clone AF3059 ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated overnight with a mouse IgG2b monoclonal antibody (clone HUTS-4) directed against active β1 integrin and α-smooth muscle actin (clone 1A4, FITC-conjugated; Sigma), followed by 1 hour incubation with anti-mouse IgG2b Alexa-647 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and fluids from the apical compartment and basal circulation were collected for the quantification of the compounds using LC-MS/MS or for the quantification of FITC-dextran (FD4, 4000 g/mol, 50 μM; Sigma-Aldrich) or FITC-inulin (3500 g/mol ...
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bioRxiv - Plant Biology 2024Quote: ... A 100-µl sample of each tissue suspension was added to 2 µl of a 1-mg/ml solution of the lectin wheat germ agglutinin (WGA) conjugated to fluorescein isothiocyanate (FITC) (Sigma Aldrich) and incubated for 30 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were blocked with HBSS buffer containing 3% BSA for 20 min followed by incubation with monoclonal anti-HA-FITC antibody (1:500) (Sigma, H7411) for 1 h at RT in the dark ...
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bioRxiv - Developmental Biology 2024Quote: ... bovine serum albumin) and incubated for a further 48 h in 1:100 fluorescein isothiocyanate (FITC)-labelled anti-rabbit secondary antiserum (Sigma Aldrich). A final overnight incubation in 1:100 TRITC-labelled phalloidin (200 ng/ml ...
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bioRxiv - Biophysics 2021Quote: Spin-labeled BmrCD mutants were concentrated to 70-100 µM using Amicon Ultra-100 kDa centrifugal filters (Millipore) and incubated with nucleotides or Hoechst-33342 ...
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bioRxiv - Biochemistry 2021Quote: ... lung or intestinal tissue were fluorescently labeled via reductive amination with 2-aminobenzamide (2-AB) (Sigma-Aldrich Canada)42 ...
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bioRxiv - Developmental Biology 2020Quote: ... the nuclei were labeled with a 1:10,000 dilution of DAPI (4’,6-Diamidino-2-phenylindole; Sigma, D9542) in PBS ...
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bioRxiv - Plant Biology 2021Quote: 2-AA-labeled N-glycans prepared for HILIC-HPLC were desalted on C18 ZipTips (Millipore, Merck, Warszawa, Poland), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Isolated nuclei were labeled by incubation with 1:1000 dilution of AlexaFluor488-conjugated anti-NeuN antibody (MAB377X, Millipore) and a 1:1000 dilution of Hoechst 33342 at 4°C for 1 hour with continuous shaking ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was labeled with mouse antiserum specific to reconstituted GFP (1:250; Sigma-Aldrich, Cat#G6539, RRID:AB_259941) [123] and either anti-serotonin (antibodies listed above).
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bioRxiv - Developmental Biology 2020Quote: ... DIG-labeled RNA probes were generated using the DIG RNA Labeling Kit SP6/T7 (Sigma-Aldrich cat. # 11175025910) according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2021Quote: ... FAK) recombinant proteins to be labeled with Alexa fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼100 μM ...
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bioRxiv - Microbiology 2020Quote: ... Soils were amended with 150 µl of either 13C7-labeled p-hydroxybenzoic acid (99% atom 13C; Sigma-Aldrich) or unlabeled control (‘12C’ ...
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bioRxiv - Cell Biology 2020Quote: ... 100 μl of cell suspension was labeled with Annexin V Alexa Fluor® 488 and Propidium Iodide (Sigma), incubated for 15 minutes at room temperature and analyzed on a BD FACSAria II (BD Biosciences) ...
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bioRxiv - Cell Biology 2022Quote: ... analyzer 1×106 asynchronously growing cells were labeled for 30 minutes with 5 μg/ml Hoechst 33342 (Sigma), trypsinized ...
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bioRxiv - Neuroscience 2021Quote: ... Detection was achieved using appropriate horseradish peroxidase-labeled secondary antibodies and Immobilon Western chemiluminescent HRP substrate (Millipore Sigma). Band intensity was determined with ImageJ 2.0.0-rc-69/1.52p software (http://imagej.nih.gov/ij ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.3% Triton) and then incubated for 1 hr 45 minutes at room temperature with Cy3-labeled phalloidin (Sigma) to label the cell contours of the epithelial cells ...
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bioRxiv - Immunology 2020Quote: ... Naïve CD4+ T cells were purified from the spleen and mesenteric lymph nodes of pTreg TN/Rag1 KO mice following the manufacturer’s instructions (Miltenyi, Germany) and labeled with 2.5 μM CFSE (Sigma) or 3μM CellTrace Violet (ThermoFischer) ...
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bioRxiv - Immunology 2023Quote: ... AP-labeled goat anti-human IgG (γ-chain specific; Sigma-Aldrich, Vienna, Austria; 1:50,000 in TBS BSA) and AP-labeled goat anti-human IgA (α-chain specific ...
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bioRxiv - Cancer Biology 2023Quote: ... asynchronously growing subconfluent KB2P1.21 or KB2P3.4 cells were labeled with 30μM thymidine analogue 5-chloro-2’-deoxyuridine (CIdU) (#C6891, Sigma-Aldrich) for 20’ ...
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bioRxiv - Cell Biology 2023Quote: ... added at the time of treatments and labeled by the click reaction with Azide Fluor 545 (Sigma Aldrich) as reported (14,19) ...
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bioRxiv - Pathology 2023Quote: ... normal fibroblasts and MMP2-overexpressing fibroblasts were labeled with the red fluorescent dye PKH26 (MINI26-1KT, Sigma-Aldrich) according to the manufacturer’s instructions and then subcutaneously injected into cultured human skin explants at a density of 5 × 106 cells/ml in 100 μl using disposable 35G needles (ReactSystem ...
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bioRxiv - Microbiology 2023Quote: ... Nuclei were labeled using 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, catalog no. D9542, 100 ng/ml). The immunostained lymph node tissues were scanned using an Olympus VS-120 slide scanner with a Hamamatsu ORCA-R2 C10600 digital camera ...
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bioRxiv - Cancer Biology 2022Quote: ... using 90 µL of acetonitrile/methanol/formic acid (74.9:24.9:0.2 v/v/v) containing stable isotope-labeled internal standards (valine-d8, Sigma-Aldrich; St ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then labeled with the next primary antibodies: mouse-anti-α-tubulin (1:500; Sigma monoclonal DM1α), rabbit-anti-PCM1 (1:250 ...
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bioRxiv - Molecular Biology 2023Quote: ... Dry weight of the <3kDa WCL fractions were obtained and 30 µg of material from each of the strains and replicates were resuspended in 30 µl of loading buffer (5% acetonitrile, 0.1% formic acid in water) containing heavy labeled RF (dioxopyrimidine-13C4,15N2, Sigma) as an internal standard at a concentration of 1 ng/µl ...
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bioRxiv - Evolutionary Biology 2023Quote: ... ctenophore cells were prepared as previously described 46,47,73 and labeled with mouse anti-histone H11-4 (EMD Millipore) and anti-mouse Alexa Fluor 488 (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2022Quote: MSC EVs from flask and bioreactor culture were labeled separately with lipid membrane PKH67 dye (Sigma-Aldrich, PKH67GL) as previously described [23] ...
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bioRxiv - Microbiology 2022Quote: ... the fixed and permeabilized cell monolayers were labeled with Phalloidin-Tetramethyl rhodamine B (Sigma-Aldrich, St. Louis, MO) for 1h at RT ...
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bioRxiv - Microbiology 2022Quote: ... Nuclei were labeled using 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, catalog no. D9542, 100 ng/ml).
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bioRxiv - Neuroscience 2022Quote: Digoxygenin (DIG)-labeled probes were produced with the SuperScriptTM III One-Step RT-PCR kit (Sigma 12574-026) using specific primers for the Bgl-FaNaC transcript (Forward ...
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bioRxiv - Cell Biology 2023Quote: RPE-1 cells pulse-labeled with fluorescent nucleotides were incubated with 1µg/mL Hoechst 33258 (Cat.# 14530, Sigma) in 1xPBS for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... 100 pg/µL of a stable isotope labeled amino acid mixture for mass spec (Sigma Aldrich; cat# 909653) and 2-Amino-3-bromo-5-methylbenzoic acid was added to each sample and vortexed briefly ...
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bioRxiv - Physiology 2024Quote: ... Each meal was labeled with either 1% rhodamine B (absorption/emission: 540 nm/575 nm) (Sigma-Aldrich R6626) or 0.002% fluorescein (487 nm/528 nm ...