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Citations for Millipore Sigma :
1651 - 1700 of 1805 citations for FITC labeled Fibrinogen since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... A 12.5 µM solution of fluorescently labeled tubulin in 1x assay buffer containing an oxygen scavenger system (freshly mixed 0.035 mg/ml catalase (Sigma Aldrich), 0.2 mg/ml glucose oxidase (Merck) ...
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bioRxiv - Cancer Biology 2023Quote: SCID mice bearing tumors generated by MDA-MB-231/Dendra2 cells at size of 1.3 cm in diameter were injected with tetramethylrhodamine-labeled 155kD dextran (Sigma-Aldrich) ten minutes before imaging ...
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bioRxiv - Bioengineering 2024Quote: ... Alexa 488-labeled phalloidin was dissolved in methanol to yield a 40X 6.6 μM stock solution whereas DAPI dihydrochloride (Sigma-Aldrich) was dissolved in UPW to yield a 100X 250 μG mL-1 stock solution ...
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bioRxiv - Immunology 2022Quote: ... Effector (TILs) and target cells (autologous tumor cell lines) were labeled respectively with 1μM PKH67 and PKH26 fluorescent dyes (Sigma-Aldrich) according to the manufacturer’s protocol and loaded on the chip array ...
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bioRxiv - Molecular Biology 2022Quote: ... 32P-labeled probes that reverse complement to the targeted miRNAs were hybridized with membrane in PerfectHyb(tm) Plus Hybridization Buffer (Sigma) overnight at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Ltk-/- and DKO mice were labeled in vivo by intraperitoneal injections with BrdU (150 µg/g body weight, Sigma-Aldrich,). In some experiments PPP (20 mg/kg body weight ...
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bioRxiv - Bioengineering 2023Quote: ... Precusor nCys- mEpoR peptide was labeled at its free amino N-terminus as a protected peptide on resin with NHS-Biotin (Sigma) by swelling the resin with DMF having 10 equivalents N,N- Diisopropylethylamine ...
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bioRxiv - Biochemistry 2022Quote: ... N-termini were labeled with light and heavy formaldehyde (12CH2O in H2O, and13CD2O in D2O (252549 and 596388, Sigma-Aldrich)) ...
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bioRxiv - Cell Biology 2023Quote: ... 12-mer Atto565-labeled or Atto647N-labeled secondary imaging oligos were diluted to 20 nM in hybridization buffer (5% ethylene carbonate (EC, Cat.# E26258, Sigma) in 2XSSC/0.2% tween) ...
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bioRxiv - Biochemistry 2023Quote: Cell lysates from cardiomyocytes treated with 10 or 50 µM H2O2 and controls were labeled with MAL-PEG (5kDa methoxy polyethylene glycol maleimide, Sigma) following a slightly modified version of a previously published procedure (15) ...
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bioRxiv - Developmental Biology 2023Quote: ... the PCR products were used as templates for in vitro transcription of DIG-labeled RNA probe using T7 RNA polymerase (SIGMA) and DIG RNA Labeling Mix (SIGMA ...
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibodies were rinsed with PBS and nuclei were labeled using DAPI (4’6-diamidino-2-phenylindole; Sigma, St. Louis, MS) in PBS (1:10,000 of 3mg/mL DAPI stock ...
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bioRxiv - Developmental Biology 2024Quote: ... Kidneys were then stained with 20 μg ml-1 AlexaFluor 488-labeled peanut (Arachis hypogaea) agglutinin lectin (PNA, L21409, Sigma) for 1 hr in RNAse-free PBS to mark ureteric bud tubules ...
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bioRxiv - Biophysics 2024Quote: ... Microtubules were labeled with anti-tubulin antibodies 1:200 in PBS (Monoclonal Anti-α-Tubulin antibody; Sigma-Aldrich, Poznań, Poland) for 24 hours and stained with anti-mouse antibodies conjugated with Alexa Fluor 555 (Goat anti-Mouse IgG (H+L ...
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bioRxiv - Microbiology 2024Quote: ... Cells transfected with poly(I:C) chemically labeled with rhodamine were fixed in methanol and stained for GAPDH (EMD Millipore, #MAB374) and DAPI (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were labeled with a rabbit anti-KIF5B antibody (Tanaka et al., 1998) and a mouse anti-insulin antibody (Sigma), followed by Alexa-conjugated secondary antibodies (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: To visualize projection neurons labeled by BDA we incubated free-floating sections for 1 h at 4°C in 0.05M glycine (Sigma-Aldrich) and preblocked for 1 h at 4°C in 5% normal goat serum (NGS ...
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bioRxiv - Microbiology 2024Quote: ... Each extract was resuspended in 170 µL of 100% methanol containing isotopically labeled internal standards (5-50 µM of 13C,15N Cell Free Amino Acid Mixture, #767964, Sigma; 1 ug/mL 2-amino-3-bromo-5-methylbenzoic acid ...
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bioRxiv - Neuroscience 2024Quote: ... and pHrodo-labeled myelin (48 µg/ml) either without or in combination with Cytochalasin D (40 µM, #C2618, Sigma-Aldrich), which inhibits phagocytosis ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were washed with phosphate buffer saline (PBS) and then binding buffer and stained with annexin V-FITC and Propidium iodide (50 µg/ml; Sigma-Aldrich, St. Louis, MO) for 15 min ...
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bioRxiv - Neuroscience 2021Quote: ... The slices were rinsed with the blocking solution and incubated with secondary antibodies for 2 h at room temperature (FITC-conjugated anti-rabbit IgG, AP132F, Millipore, Darmstadt, Germany; 1:200). The slices were then washed three times with PBS and mounted with 4’,6-diamidino-2-phenylindole (DAPI)-containing Vectashield (Vector Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... anesthetized with 5% isoflurane and retro-orbitally injected with 100 μL of 5% (weight/volume) fluorescein isothiocyanate–dextran (FITC) (FD150S, Sigma-Aldrich, St. Louis, MO) dissolved in sterile saline ...
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bioRxiv - Microbiology 2022Quote: ... 2 µM FITC-TB_KKG6K was added along with 5 μM of the cell wall specific fluorescent dye calcofluor white (CFW; Sigma-Aldrich, St. Louis, MO, USA) to 800 μL of the C ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then washed multiple times with PBSTx before being incubated in FITC-conjugated goat anti-mouse secondary antibody (Sigma cat. #A6667, 1:200) for 2-5 hr at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... WI-38 or LF-IPF cells were stained with FITC-conjugated mouse monoclonal anti-ACTA2 antibodies at 1:200 dilution (Sigma-Aldrich, cat. nr F3777) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... mice were deprived of water for 4hrs and orally administered 200 ml of PBS containing 8 mg of FITC-dextran (4000 Da; FD4, Sigma Aldrich, Saint Louis, MO) per 20 g of body weight ...
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bioRxiv - Microbiology 2022Quote: ... mice were gavaged with 0.2 ml of Fluorescein Isothiocyanate-Dextran (FITC-Dextran; 3–5 kDa; cat. no. FD4; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) in PBS ...
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bioRxiv - Immunology 2022Quote: ... and then incubated for 1 hour at 37°C with secondary goat anti-rabbit antibodies conjugated with fluorescein isothiocyanate (FITC; 1:250; Sigma-Aldrich, Prague, Czech Republic). Fluorescence intensity was measured using the Synergy H1 microplate reader (BioTek ...
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bioRxiv - Molecular Biology 2024Quote: ... individual ligand was added into the upper well of the inserts for 30 minutes followed by replacing the medium with 150 µL of FITC-Dextran (1 mg/mL, Sigma-Aldrich, 20 kDa, #FD20S) containing medium into the inserts ...
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bioRxiv - Microbiology 2024Quote: ... the cells were exposed to 200 μL of antibiotic-free DMEM containing PMT at a final concentration of 25 µg/mL and 70 kDa fluorescein isothiocyanate (FITC; Sigma, St. Louis, MO, USA) at a final concentration of 10 µM.
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bioRxiv - Cell Biology 2024Quote: The acrosome status of sperm was assessed using 25 µg/mL fluorescein isothiocyanate- conjugated peanut agglutinin (FITC-PNA; L7381, Sigma Aldrich, St. Louis, MO) and 2.4 nM propidium iodide (PI ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were then labeled with the PLA antimouse PLUS probe (DUO92001) and the PLA anti-rabbit MINUS probe (DUO92005) purchased from Sigma-Aldrich. Detection was performed with Duolink in situ detection reagents red kit (DUO92008 ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin- or fluorescein-labeled riboprobes were synthesized as described (Pearson et al., 2009) and detected with anti-digoxigenin-HRP (1:2000, Roche/Sigma-Aldrich 11207733910 ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were fluorescently labeled using methyl green (Prieto et al, 2014) and actin filaments with TRITC-conjugated phalloidin (Sigma-Aldrich, P1951). Observation of whole embryos was performed using a Zeiss LSM 880 or a Zeiss LSM 800 laser confocal microscope ...
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bioRxiv - Molecular Biology 2020Quote: HPG labeled proteins in supernatant and pellet fractions (∼1 mg) were click-conjugated with 50 µM azide-PEG3-biotin (Sigma-Aldrich) by a Click-iT Cell Reaction Buffer Kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1 μL of DIG labeled probe was diluted in 100 μL prehybridization buffer containing 400 ng tRNA (Sigma-Aldrich, St. Louis) and denatured at 70°C for 5 min ...
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bioRxiv - Biophysics 2021Quote: ... flat 1:30 PDMS stamps were incubated for 1 h with 40 mL of 50 mg/mL Alexa Fluor 647–labeled and 50 mg/mL unlabeled fibronectin (F1141; Sigma-Aldrich), then washed and dried ...
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bioRxiv - Biochemistry 2020Quote: Rhodamine-labeled microtubules were polymerized from bovine tubulin (~10% final rhodamine-labeled tubulin, prepared as previously and stabilized with 10 μM taxol (Sigma T7191) before being pelleted in a TLA120.1 rotor at 90,000 rpm for 10 min at 25°C (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... Then the particles were re-suspended in 50 mM pH 7.5 HEPES buffer, labeled with self-healing Cy3 and Cy5 derivatives (LD555-CDand LD650-CoA, respectively) and purified through an OptiprepTM (Sigma Aldrich) gradient as previously described (Lu et al. ...
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bioRxiv - Cell Biology 2021Quote: ... 100 nM of the specified SPR2-GFP proteins along with 2.5 μM 1:9 rhodamine-labeled porcine tubulin were then introduced into the flow chamber in BRB80 buffer containing 1% methyl cellulose (4000 cP, Sigma-Aldrin), 50 mM DTT and an oxygen-scavenging system consisting of 250 μg/ml glucose oxidase ...
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bioRxiv - Cell Biology 2021Quote: ... Microtubule polymerization was initiated by flowing in 20 mM 1:25 rhodamine-labeled porcine tubulin in BRB80 buffer containing 1% methyl cellulose (4000 cP, Sigma-Aldrich), 50 mM DTT ...
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bioRxiv - Cell Biology 2021Quote: ... The blot was then neutralized with SSC and hybridized with a DIG-labeled telomere probe prepared as described 48 in DIG Easy Hyb (Sigma-Aldrich) solution overnight at 42°C ...
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Stress response in Entamoeba histolytica is associated with robust processing of tRNA to tRNA-halvesbioRxiv - Microbiology 2021Quote: ... Probe DNA was 5′-end labeled by PNK reaction using γ-[32P]-ATP and hybridized with the membrane in perfectHyb buffer (Sigma) overnight at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were next labeled as follows: HA-tagged proteins were detected by anti-HA rat monoclonal antibody 3F10 (Sigma, 1:200), parasite cyst wall and cyst matrix by in-house SalmonE anti-CST1 antibody (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... The FISH hybridization mixture (20 ng of the labeled probe, 10 μg of salmon sperm, 50% of deionized formamide (Sigma-Aldrich)) in 2 x SSC was incubated on slides at 82 °C for 5 min ...
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bioRxiv - Immunology 2022Quote: ... CHO CoV-2-Spike cells were used as target cells and were fluorescently labeled with PKH67 Fluorescent Cell Linker Kit (Sigma Aldrich) prior to incubation with mAbs ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were fixed with 4% paraformaldehyde and stained with mitochondrial networks labeled with a primary antibody against TOMM20 (Sigma-Aldrich, HPA011562) used at 1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti-sense DIG-labeled in-situ probes were generated using linearized plasmid cDNA templates with 10X DIG RNA labeling mix (Sigma 11277073910) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Anti-sense DIG labeled in- situ probes were generated using linearized plasmid cDNA templates with the10X DIG RNA labeling mix (Sigma 11277073910) according to manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... 5 × 106 activated B cells were washed with PBS and re-cultured in glucose-free medium RPMI medium containing 10% dialyzed FBS and uniformly labeled [13C]-glucose (2 g/L; Sigma-Aldrich) for 24 and 48 h ...