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Citations for Millipore Sigma :
101 - 150 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... MAG (Anti-Myelin Associated Glycoprotein, clone 513 antibody, Millipore mab1567 ...
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bioRxiv - Immunology 2020Quote: ... and sheep serum (Sigma) was used ...
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bioRxiv - Microbiology 2022Quote: ... were resuspended in 100 μL of FC buffer containing primary antibodies (mouse anti-Spike S2, Thermo Fisher, Cat #: MA5-35946, 1:500 dilution) (mouse anti-Flag M2, Sigma, Cat #: F1804, 1: 500 dilution), or anti-SARS-CoV-2 human sera (1:100 dilution ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
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bioRxiv - Developmental Biology 2022Quote: ... 2% FCS and 0.5µg/ml DAPI (Sigma Aldrich, Ref: D9542) and passed through a 40µm Falcon cell strainer ...
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bioRxiv - Biochemistry 2021Quote: HEK293 cells (Sigma:85120602) were cultured using DMEM (Gibco™ ...
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bioRxiv - Biophysics 2023Quote: HEK293 cells (Sigma Aldrich) and were cultured in DMEM (Gibco ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The rehydrated slides were blocked with 2% horse-serum followed by incubation with primary antibodies (Mouse anti-S-glycoprotein antibody, 1:200 dilution, Millipore, Cat # MAB5676 ...
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bioRxiv - Immunology 2020Quote: ... and primary antibodies in the presence of Fc block in flow buffer (DPBS [Gibco] supplemented with 2% FCS, 2 mM EDTA [both Sigma] and 0.01% sodium azide [Morphisto]). After staining with secondary biotin-coupled antibodies in flow buffer ...
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bioRxiv - Microbiology 2020Quote: ... The flow-through from the His-tag cleavage containing the tag free protein was concentrated to 2 mL using Amicon Ultra-4 30k MWCO (Millipore). The concentrated protein was applied to a HiLoad 16/600 S75pg column (GE Life Sciences) ...
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bioRxiv - Immunology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2022Quote: ... and −7173 of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2023Quote: ... and -71)60 of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Immunology 2021Quote: ... pH 6.5 and glycoproteins oxidized with 1.6 mM NaIO4 (Sigma) in PBS ...
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bioRxiv - Plant Biology 2022Quote: ... equivalent blots were stained with Glycoprotein Detection Kit (Sigma-Aldrich) or probed with anti-GFP monoclonal Ab (SI sections 4 ...
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bioRxiv - Immunology 2024Quote: ... a glycoprotein mucin standard derived from porcine stomach (Sigma-Aldrich) and BALF samples were diluted 2-fold serially with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... sheep anti-mouse (Sigma #A5906) or goat anti-rat (Sigma #AP136P ...
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bioRxiv - Developmental Biology 2023Quote: ... and 5% sheep serum (Sigma) diluted in MABT ...
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bioRxiv - Developmental Biology 2023Quote: ... and 5% sheep serum (Sigma) diluted in maleic acid buffer with 0.1% tween-20 (MABT) ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 2% (v/v) FCS and 2mM EDTA (Sigma, E7889). Wash buffer consists of PBS supplemented with 2% FCS.
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bioRxiv - Biochemistry 2022Quote: ... residual bound Coa and Efb were detected with anti-tag HRP-conjugated antibodies diluted 1:10000 in 2%BSA-PBST: α-HIS-tag antibody (A7058, Sigma) for EfbN ...
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bioRxiv - Microbiology 2020Quote: ... 1:2000 (SARS-1-S, MERS-S and SARS-2-S) or 1:5000 (229E-S)] and mouse anti-β-actin (Sigma-Aldrich, A5447, 1:5000). As secondary antibody ...
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bioRxiv - Biochemistry 2023Quote: ... the Siglec-7-Spy Tag-TEV-Fc was concentrated using an AMICON (Molecular Weight Cut Off 30 kDa – Merck Millipore) to approximately 0.15 mg/ml measured via NanoDrop as described above ...
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bioRxiv - Biochemistry 2021Quote: HEK293 and HEK293T cells (Sigma) were cultured at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Cultured human embryonic kidney 293 (HEK293) cells were transfected using 2 μg/μl polyethylenimine (Sigma-Aldrich) with indicated plasmids for the expression of either Flag-KCTDs alone or in combination with Flag-Cav2.3 ...
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bioRxiv - Immunology 2020Quote: 2×105 HEK293 were seeded onto 24well plates previously pre-coated with poly-L-lysine (Sigma), and infected with 2×105 ...
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bioRxiv - Biochemistry 2020Quote: Alpha-1-acid glycoprotein (AGP) purified from human serum (Sigma #G9885) and A/Philippines/2/1982 (Phil82 ...
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bioRxiv - Immunology 2021Quote: ... The high mannose glycoproteins subsequently were treated with endoglycosidase F1 (Millipore) to obtain homogeneously deglycosylated RBD.
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bioRxiv - Immunology 2023Quote: Myelin oligodendrocyte glycoprotein(MOG)-35-55 peptide(Sigma-Aldrich,100µg/ml), IFNγ(10ng/ml;Cat#315-05,PeproTech ...
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bioRxiv - Biochemistry 2023Quote: ... The dried glycoproteins were reduced using 1,4-dithiothreitol (Sigma; 0.18 M) followed by carboxymethylation using iodacetamide (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... Glycoproteins were digested with 1:25 Proteinase K (PK; Sigma-Aldrich) for 30 min at 37□C (For NVX-CoV2373 ...
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bioRxiv - Bioengineering 2023Quote: ... mouse anti-P-glycoprotein (Sigma-Aldrich, cat# 517310, AB_564389, 1:100), rabbit anti-P-glycoprotein (Abcam ...
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bioRxiv - Immunology 2023Quote: ... harvested neutrophils were resupended in PBS + 2% FCS and Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). Cells were stained with CD15-APCCy7 (1:100 ...
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bioRxiv - Immunology 2023Quote: ... harvested neutrophils were resupended in PBS containing 2% FCS and Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). Cells were stained with the following antibodies ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used are as follows: Anti-poly-ADP-ribose binding reagent/ PAR reagent (recombinant protein fused to rabbit Fc tag; Millipore, MABE1031), Rabbit monoclonal anti-Poly/Mono-ADP Ribose (anti-PAR/MAR ...
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bioRxiv - Microbiology 2022Quote: ... an overlay of DMEM containing 2% FCS and 0.6% Methylcellulose (Sigma-Aldrich) was added to the cells ...
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bioRxiv - Immunology 2020Quote: ... The pellet was resuspended in IMDM containing 2% FCS and formaldehyde (Sigma) was added to a final concentration of 1% ...
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bioRxiv - Immunology 2019Quote: ... The pellet was resuspended in IMDM containing 2% FCS and formaldehyde (Sigma) was added to a final concentration of 1% ...
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bioRxiv - Immunology 2021Quote: ... with 10% FCS and 2 mM L-Glutamine (Sigma, Cat No. G7513) and 1% penicillin/streptomycin (Invitrogen cat no ...
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bioRxiv - Neuroscience 2022Quote: ... sheep anti-TH (Chemicon/Sigma, AB1542), rabbit anti-AVP (19).
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bioRxiv - Neuroscience 2019Quote: ... sheep anti-tyrosine hydroxylase (Millipore AB1542)) 1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Sheep anti-mouse Cy3 (Sigma C2181) and goat anti-rat Alexa Fluor 488 (Invitrogen A11006 ...
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bioRxiv - Neuroscience 2023Quote: ... sheep anti-tyrosine hydroxylase (Millipore AB1542)) 1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... BioLegend),Tyrosine Hydroxylase (sheep, 1:200, Millipore). Secondaries and dilutions are as follows ...
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bioRxiv - Immunology 2019Quote: ... in FACS buffer (PBS supplemented with 2% FCS and 2 mM (Ethylenedinitrilo)tetraacetic acid (EDTA) (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2021Quote: ... HEK293 cells (2 x 104 cells per well) were transiently transfected using X-tremeGENE™ 9 (Sigma) with plasmids encoding eYFP tagged PAR2 (PAR2-eYFP ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 WT (Sigma Cat. Nr. 85120602), HEK293 GALNT1 KO (Narimatsu et al. ...
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bioRxiv - Immunology 2021Quote: ... HEK293 cells (Sigma Aldrich 12022001-1VL) were maintained in DMEM supplemented with L-glutamine and 10% FBS ...