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Citations for Millipore Sigma :
51 - 100 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 400 μl containing 300 PFU/ml of SARS-CoV-2 virus were then added to the mAb solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Immunology 2020Quote: ... 0.5 mL of SARS-CoV-2 S-2P protein was mixed with either an equal volume of Sigma Adjuvant System S6322 (Sigma), 600 μg/mL Adju-Phos aluminum phosphate (Brenntag) ...
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bioRxiv - Immunology 2021Quote: ... 400 μl containing 500 PFU/ml of SARS-CoV-2 virus were then added to the Ab solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Immunology 2022Quote: ... CHO CoV-2-Spike cells were used as target cells and were fluorescently labeled with PKH67 Fluorescent Cell Linker Kit (Sigma Aldrich) prior to incubation with mAbs ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich. Anti-influenza A virus nucleoprotein (NP ...
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bioRxiv - Immunology 2020Quote: ... Blotted proteins were detected with a monoclonal antibody that recognizes SARS-CoV-2 S1 (SinoBiological) and a secondary anti-rabbit horse radish peroxidase (HRP)-conjugated antibody (Sigma Aldrich). Blots were developed with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ADCP assay was performed using ExpiCHO cells stably expressing full-length wild-type SARS-CoV-2 S (target cells) and labelled with PKH67 (Sigma Aldrich) as targets ...
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bioRxiv - Immunology 2022Quote: ... 1 – 1.5 million cells were plated into 96-well V-bottom plates in 200mL R10 and stimulated with SARS-CoV-2 peptide pools (2ug/mL for each peptide) in the presence of Brefeldin A (Sigma-Aldrich) and monensin (GolgiStop ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... and analyzed by western blot with anti-SARS-CoV-2 N protein monoclonal antibody (0.5 μg/mL, anti-SARS-CoV-2 N mAb, clone 1G10C4 mAb)42 and anti-β-actin antibody (Sigma-Aldrich, cat ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-SARS-CoV-2 proteins or lacking the fusion protein (∆Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland) and HTCC-77 (100 μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed and then sequentially incubated with anti-SARS-CoV-2 CR3022 mAb [24] (1 μg/mL) and a HRP-conjugated goat anti-human IgG (Sigma, Cat#A6029) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Immunology 2023Quote: ... The first kit used was the Milliplex® SARS-CoV-2 antigen panel 1 IgA assay (Millipore Sigma, Cat No. HC19SERA1-85K) with the Wuhan-1 strain RBD and N proteins included ...
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bioRxiv - Immunology 2019Quote: ... and 2% FCS (Sigma) adjusted to pH 7.4 at 37°C and sterile filtered) ...
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bioRxiv - Microbiology 2020Quote: ... 2% FCS (Sigma-Aldrich), 20 mM HEPES buffer (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... were transfected with a pDisplay vector encoding amino acids 16 to 682 of SARS-CoV-2 S (S1) fused to a C-terminal hemagglutinin (HA) peptide using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). The supernatant was collected six days post-transfection ...
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bioRxiv - Immunology 2022Quote: Stock concentration of VSV-SARS-CoV-2 at a concentration of ~150 µg/mL viral RNA was conjugated with Atto565-NHS ester (Sigma-Aldrich, cat.72464) as previously described (32 ...
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bioRxiv - Microbiology 2021Quote: ... were transfected with a pDisplay vector encoding amino acids 16 to 682 of SARS-CoV-2 S (S1) fused to a C-terminal hemagglutinin (HA) peptide using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). Supernatant was collected 6 days post-transfection ...
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bioRxiv - Immunology 2020Quote: ... a pooled rhesus serum collected day 14 after infection with SARS CoV-2 was used as standard after depletion of IgG using GE Healthcare Protein G Sepharose (Sigma, St. Louis, MO) as described (52) ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells were infected with a MOI 0.002 (250 plaque forming units (PFU)) of SARS-CoV-2 for 1hr before overlaying with 1% methylcellulose- viscosity (4,000 centipoises) (Sigma- Aldrich, St. Louis, MO). Cells were incubated for 4 days at 37°C before fixing in 8% formaldehyde and stained with 1% crystal violet to visualize plaques ...
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bioRxiv - Immunology 2020Quote: ADCP assays were performed using ExpiCHO-S target cells transiently transfected with SARS-CoV-2 S and fluorescently labeled with PKH67 Fluorescent Cell Linker Kits (Sigma Aldrich, Cat. Nr.: MINI67) as targets ...
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bioRxiv - Immunology 2022Quote: ... containing 2% FCS (Sigma Aldrich), 0.1 mg/ml DNase I (Sigma Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... containing 2% FCS (Sigma-Aldrich), 20 mM HEPES buffer (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... containing 2% FCS (Sigma-Aldrich), 100 μg/ml gentamycin (Gibco ...
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bioRxiv - Developmental Biology 2020Quote: ... blocked in 2% sheep serum (Sigma, St. Louis, MO), 2 mg ml−1 bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2019Quote: ... pTAC-MAT-Tag-2 (Sigma-Aldrich) was PCR-amplified with primers pTAC-Gbs-for/rev (Table S9) ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Microbiology 2021Quote: ... the Fc tag of all APN orthologs was removed using thrombin (Millipore Sigma) in a reaction mixture containing ...
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 anti-RBD and N IgG antibody levels were determined using the Milliplex® SARS-CoV-2 antigen panel 1 IgG assay (Millipore Sigma, Cat No. HC19SERG1-85K) with the Wuhan-1 strain RBD and N proteins included ...
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bioRxiv - Biochemistry 2021Quote: Three BALB/c mice were immunized subcutaneously with 5 μg pre-fusion stabilized MERS-CoV S2 and 20 μg of ODN1826 + 100 μl of 2X Sigma Adjuvant System (SAS; Sigma) containing monophosphoryl lipid A and trehalose dimycolate in squalene oil ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV effector cells were washed twice with PBS and incubated with 3μg/ml of TPCK-treated trypsin (Sigma-Aldrich) for 30 mins at 37°C before resuspending in phenol red-free DMEM-10% ...
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bioRxiv - Molecular Biology 2024Quote: HEK293 and Caco-2 cells were grown in DMEM culturing medium (Sigma) with 10% FBS (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 2% Fetal Calf Serum (FCS (Sigma) or STEMCELL Technologies (SCT)) ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transfected with pCG1-SARS-CoV-2_S plasmid using X-tremeGENE™ 9 DNA transfection reagent (Millipore-Sigma: 06365779001) (ratio 3:1 μl/μg DNA) ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transfected with pCG1-SARS-CoV-2_S plasmid using X-tremeGENE™ 9 DNA transfection reagent (Millipore-Sigma: 06365779001) (ratio 3:1 μl/μg DNA) ...
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bioRxiv - Microbiology 2020Quote: ... stained for VZV glycoprotein E (gE) (MAB8612, Millipore) in BD PermWash ...
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bioRxiv - Cell Biology 2019Quote: ... affinity purification for glycoproteins using Con A (Sigma) was performed overnight ...
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bioRxiv - Neuroscience 2021Quote: ... myelin associated glycoprotein (#MAB1567; MAG; Sigma; 1:500), and the CSPG brevican (#SC-135849 ...
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bioRxiv - Neuroscience 2023Quote: ... MOG (myelin oligodendrocyte glycoprotein, Cat#MAB5622, Merck Millipore) and NFH (neurofilament heavy chain ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells stably expressing CCR5 were labeled with 2ug/ml rabbit anti-Flag tag antibody (F7425, Sigma, St. Louis, MO, USA) in FACS buffer (PBS with 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Immunology 2022Quote: ... and spike protein-bound antibodies were detected using an Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich, #A2429) at a 1:4000 dilution in blocking buffer ...
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bioRxiv - Immunology 2021Quote: ... with 2% foetal calf serum (FCS, Sigma-Aldrich, UK). Lymphoprep (d = 1.077 g mL−1 ...
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bioRxiv - Immunology 2023Quote: ... mixed in 2% fetal calf serum (FCS; Sigma-Aldrich). Cells were stained with combinations of the following fluorophore-conjugated anti-mouse monoclonal antibodies ...
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bioRxiv - Microbiology 2023Quote: ... a BiP secretion sequence and a C-terminal C-tag) was expressed from a stable S2 cell line34 (ExpreS2ion Biotechnologies) in EX-CELL® 420 Serum Free Medium (Sigma Aldrich). After 3-4 days ...
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bioRxiv - Immunology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Immunology 2021Quote: ... or 900-fold (SARS-CoV-1) diluted plasma samples before addition of THP-1 cells (20,000 cells per well; Millipore Sigma, Burlington, MA). After 19 h incubation at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich, cat. C2306). F-actin and DNA staining were performed by incubating the embryos in 1:100 BODIPY FL-Phallacidin (Life Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich, cat. C2306). F-actin and DNA staining were performed by incubating the embryos and larvae in 1:100 BODIPY FL-Phallacidin (Life Technologies ...