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Citations for Millipore Sigma :
101 - 150 of 10000+ citations for Mayaro Virus E2 Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... the Siglec-7-Spy Tag-TEV-Fc was concentrated using an AMICON (Molecular Weight Cut Off 30 kDa – Merck Millipore) to approximately 0.15 mg/ml measured via NanoDrop as described above ...
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bioRxiv - Biophysics 2021Quote: Fibrinogen (human plasma purified protein, Sigma-Aldrich)
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bioRxiv - Cancer Biology 2019Quote: ... 17β-estradiol (E2) were purchased from Sigma Aldrich (MO, USA). Routine laboratory buffers ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 nM 17-beta estradiol (E2) (all from Sigma-Aldrich) and 2.5 mIU/ml FSH (Merck ...
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bioRxiv - Developmental Biology 2023Quote: ... 17D-estradiol (E2) (E8875, Sigma-Aldrich, St. Louis, MO, USA) and progesterone (P4 ...
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bioRxiv - Molecular Biology 2023Quote: ... 17β-estradiol (E2) and lasofoxifene (Las) were purchased from Sigma, 4-hydroxytamoxifen (4-OHT) ...
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bioRxiv - Molecular Biology 2024Quote: ... The hormones used were as follows: 17β-estradiol (E2; Sigma) and recombinant human relaxin-2 (Rln ...
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bioRxiv - Cell Biology 2019Quote: ... S-protein-agarose beads and S-tag antibody were purchased from EMD Millipore. Endo H ...
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bioRxiv - Biophysics 2021Quote: ... Protein was expressed as a His6-tag fusion in BL21(DE3) trxB (Novagen) using autoinduction24 ...
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bioRxiv - Microbiology 2021Quote: ... Putative ligand proteins (elastin from human skin, fibrinogen from human plasma, laminin from human placenta, fibronectin from human plasma) (all from Sigma) were resuspended in carbonate-bicarbonate buffer (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... and anti-Human IgG (Fc specific) −Peroxidase antibody produced in goat was from Sigma-Aldrich. Two TNFα-antagonists were purchased ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μL of goat anti-human IgG (Fc specific)-Peroxidase antibody (1 : 5000 dilution, Sigma) was added and incubated for another 1 h at 37 °C ...
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bioRxiv - Biophysics 2023Quote: ... The anti-human Fc capturing mAb (GG-7) was from Sigma-Aldrich (St. Louis, MO). LIBS-2 was purchased from EMD Millipore (Billerica ...
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bioRxiv - Bioengineering 2023Quote: ... 100 µL of anti-human IgG (Fc specific)-peroxidase antibody produced in goat (Sigma, A0170) was added to each sample in a 1:40,000 dilution of PBS and incubated for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHH-Fcs or conventional human monoclonal antibodies was detected by HRP-conjugated rabbit anti-human IgG (Sigma, A8792, 1/2000). After washing 50 μL of TMB substrate (Tetramethylbenzidine ...
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bioRxiv - Microbiology 2019Quote: ... Fc chimeric proteins were purified via affinity based Fast Protein Liquid chromatography using Prosep® Ultra resin (Millipore). Fc conjugated proteins were eluted with 0.1 M glycine (pH 2.5 ...
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bioRxiv - Genetics 2021Quote: ... Each ORF cloned into the pLVX-EF1a-IRES-PURO/eGFP lentiviral expression vector was added to lentiviral packaging and envelope vectors (Sigma-MISSION-gag-pol and the envelope Sigma-MISSION-VSV-G (Cat: CST-DNA Sigma, St-Louis, MO)) in a 2:2:1 ratio and co-transfected into HEK-293T packaging cells via the calcium phosphate precipitation method according to the Open-Biosystem protocol (as described in (23)) ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated from media using PEG Virus Precipitation Kit (Sigma). Viral titer was determined by QuickTiter™ Lentivirus Associated HIV p24 Titer Kit (Cell Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... and the GST tag was cleaved by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... coli BL21(DE3) as a fusion protein containing an N- terminal His6-tag (Novagen) and purified by metal-affinity chromatography (Ni-NTA ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Developmental Biology 2019Quote: ... were incubated in water containing 17β-estradiol (E2; E1132, Sigma-Aldrich) at 1 nM ...
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bioRxiv - Microbiology 2022Quote: ... E2 and P4 were solubilized in 2-butanol (2-BtOH; Sigma) as our previous studies demonstrate that this reagent does not interfere with NgPLD activity ...
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bioRxiv - Cancer Biology 2019Quote: 17β-estradiol (E2) was purchased from Sigma Aldrich (St. Louis, MO). DIM used in cell culture was purchased from Sigma Aldrich (St ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were treated with 10 nM of 17β-estradiol (E2; Sigma) and/or 10 nM of letrozole (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... mice were injected with 100 ng of estrogen (E2; Sigma-Aldrich) dissolved in 100 µL of sesame oil on two consecutive days and then allowed to rest for two days ...
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bioRxiv - Biochemistry 2023Quote: ... Bisphenols and E2 were dissolved in DMSO (Sigma-Aldrich, Madrid, Spain).
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bioRxiv - Systems Biology 2023Quote: ... supplemented with 5 nM estradiol-17β (E2) (Sigma, Cat. no. E2257) and 50 µg/mL Ascorbic acid (ATCC ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 nM 17β-estradiol (E2, E; Sigma, St. Louis, MO, USA), 0.2 μg/mL recombinant human relaxin-2 in 0.1% BSA-PBS (Rln ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then treated with 1 µM estradiol (E2, Sigma Aldrich) for 30 minutes before treating with 1 µM of 5-Ph-IAA (Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... Plasminogen (human plasma purified protein, Sigma-Aldrich, Germany) and Fibrinogen (human plasma purified protein ...
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bioRxiv - Biophysics 2021Quote: ... and Fibrinogen (human plasma purified protein, Sigma-Aldrich). Experimental conditions are presented in the table I.
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bioRxiv - Biophysics 2021Quote: Plasminohen (human plasma purified protein, Sigma-Aldric, Germany)
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bioRxiv - Developmental Biology 2019Quote: ... 200nL 0.1µg/µL human noggin protein (Sigma H6416) was injected into the blastocoel of embryos at stage 8 ...
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bioRxiv - Neuroscience 2019Quote: ... and His-tagged human HDAC6 protein (EMD Millipore) were incubated with 57 nm 32P-ATP in kinase buffer (50 mM Tris HCl ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated with FITC-conjugated goat anti-human IgG (Fc specific) (Sigma St-Louis, MI) or FITC-conjugated goat anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: Human cells (HeLaK and Hek293T) were cultured in DMEM (Biowest) supplemented with 10% FCS (Sigma-Aldrich) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2019Quote: The genes of either E protein or E DIII protein (residues 301-403) of ZIKV (GZ01, KU820898) without tag were cloned into pET28a vectors (Novagen) and expressed by IPTG-induction in BL21 (RIL ...
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bioRxiv - Biophysics 2021Quote: ... The GB1 tag was cleaved from the oxidized proteins by incubating overnight with thrombin (10 U.mg−1 protein, Sigma-Aldrich). The cleaved protein was purified by reversed phase high-performance liquid chromatography (RP-HPLC ...
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bioRxiv - Microbiology 2023Quote: ... strep tag and myc tag were obtained from Sigma, IBA and Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Neuroscience 2019Quote: ... recombinant ephrins or Fc were preclustered in a 5:1 molar ratio with either mouse or goat anti-human Fc (Millipore Sigma cat. no. 16760 and 12136 respectively) for 30’ at 37°C.
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bioRxiv - Immunology 2020Quote: NTD-Fc or RBD-Fc proteins were diluted in sterile PBS and formulated as an emulsion with TiterMax Gold adjuvant (Sigma-Aldrich, St. Louis, MO) to a final antigen concentration of 250 µg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... or E2+DFP (80 mg/kg daily, Sigma–Aldrich, St. Louis, MO) for eight weeks from week nine as the early OVX group or week 21 as the late OVX group.
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bioRxiv - Genomics 2022Quote: ... fresh media containing 10 nM E2 and 1 µM medroxyprogesterone acetate (Sigma) was added ...
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bioRxiv - Developmental Biology 2022Quote: ... we treated the mutant fish with E2 (Sigma-Aldrich, St. Louis, MO) followed by sampling for histological analysis ...
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bioRxiv - Cancer Biology 2021Quote: ... and 10-8M 17-ß-estradiol (E2, Sigma-Aldrich, Saint-Louis, USA) and were kept at 37°C with 5% CO2 in a humidified atmosphere ...
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bioRxiv - Cancer Biology 2020Quote: ... and 10-8M 17-ß-estradiol (E2, Sigma-Aldrich, Saint-Louis, USA) and were kept at 37°C with 5% CO2 in a humidified atmosphere ...
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bioRxiv - Immunology 2022Quote: ... cells were treated with 10 nM of 17β-estradiol (E2; Sigma-Aldrich) and collected after a 36 hr incubation according to previously established protocols [21].
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bioRxiv - Developmental Biology 2024Quote: ... The E2/P4 mice received 1 mg of progesterone (P4; Sigma-Aldrich) for three consecutive days followed by a single injection of 1 mg P4 plus 50 ng E2 on the fourth day ...