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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for Mayaro Virus E2 Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: The C19orf43.WT plasmid is comprised of an N-terminal 3x Flag tag followed by human full length C19orf43 cloned into the p3XFlag-CMV-10 vector (Sigma). C19orf43.R7G was obtained by mutating the arginine at position 7 to glycine and C19orf43.R106G was obtained by mutating the arginine at position 106 to glycine ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908, Sigma, 1:1,000). The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012 ...
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bioRxiv - Genetics 2021Quote: ... mice were administered E2 (E8875, Sigma-Aldrich, St. Louis, MO; 100 ng per mouse) as daily injections for 3 days ...
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bioRxiv - Genomics 2020Quote: ... cells were treated in triplicate with 17β-estradiol (E2, E2758, Sigma, St. Louis, MO) in pfDMEM supplemented with 15% charcoal/dextran treated FBS and 1% penicillin/streptomycin under pulsed or continuous dosing ...
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bioRxiv - Genomics 2022Quote: ... at 70–80% confluency for 2 days before treatment with 50 nM E2 (SIGMA) for the indicated times ...
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bioRxiv - Physiology 2022Quote: ... we used two different doses of E2 (10 nM, 100 nM; Sigma-Aldrich, USA) and PGE2 (1 uM ...
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bioRxiv - Neuroscience 2024Quote: ... and the proinflammatory cytokine prostaglandin-E2 (PGE2) from Sigma-Aldrich (St. Louis, MO, USA). The selection of drug doses was based on dose-response relationships determined during earlier studies (28 ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 5 nM female sex hormone estradiol-17β (E2) (Sigma, Cat. no. E2257) to promote epithelial differentiation and 50 µg/mL Ascorbic acid (ATCC ...
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bioRxiv - Cell Biology 2023Quote: ... with or without E2 (10-8 M; E1024, Sigma-Aldrich, St. Louis, MO, USA) for 3 ...
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bioRxiv - Cell Biology 2023Quote: ... with or without E2 (10-8 M; E1024, Sigma-Aldrich, St. Louis, MO, USA) for 3 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were treated with DMSO (vehicle) or 100 nM 17β-estradiol (E2) (Sigma, E8875) for the indicated times ...
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bioRxiv - Cell Biology 2019Quote: ... Doxycycline-inducible OKSM virus (Millipore, SCR512) and m2rtTA virus (Cyagen Biosciences ...
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bioRxiv - Bioengineering 2019Quote: ... Lyophilized proteins were purchased: human serum albumin (HSA; from human plasma, ≤0.02% Fatty acids, Lot #SLBZ2785, Millipore Sigma) and fibrinogen (FBG ...
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bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted by cleavage of the GST tag by 15 units/ml TEV protease (Sigma T4455-1KU) in PBS buffer overnight at 4 °C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The protein composition of the eluent was verified by western blot using Strep-tag II monoclonal antibodies (Novagen). The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The protein composition of the eluent was verified by western blot using Strep-tag II monoclonal antibodies (Novagen). The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius ...
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bioRxiv - Cell Biology 2024Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
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Structure and Neutralization Mechanism of a Human Antibody Targeting a Complex Epitope on Zika VirusbioRxiv - Microbiology 2022Quote: ... Plates were washed with TBS containing 0.2% Tween and incubated for 1 hour at 37°C with Goat anti-human Fc (Sigma, A9544) or Fab (Jackson immunoResearch AB_2337617 ...
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bioRxiv - Cell Biology 2021Quote: ... plates were washed 4 times with PBST and further incubated with 100 μL per well of goat anti-human IgG (Fc specific)-Peroxidase antibody (1: 5000 dilution, Sigma) for 1 h at 37°C ...
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bioRxiv - Microbiology 2020Quote: rAkata EBV-infected cells was reactivated at 1×106 cells/mL with a goat polyclonal anti-human IgG Fc-specific antibody (Sigma) for 48 hours ...
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bioRxiv - Neuroscience 2024Quote: ... the plate was washed 5 times with PBS and incubated for 1 hour with goat anti-human IgG (Fc specific) –peroxidase (#A0170, Sigma). Binding signals were developed and stopped by the addition of TMB (3,3’,5,5’-tetramethylbenzidine ...
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bioRxiv - Pathology 2022Quote: ... the virus particle-containing media was harvested and virus particles were purified using Polyethylene glycol 8000 (Sigma).
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Biochemistry 2024Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were selected by growth in puromycin (E2: 1µg/ml, G7: 0.5µg/ml; Sigma Aldrich) or blasticidin (G7 ...
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bioRxiv - Cancer Biology 2019Quote: ... 100 μg/mL Streptomycin and 1nM β-estradiol (E2) (Sigma, E2758, St. Louis, MO, USA) at 37°C and with 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... the mice received a subcutaneous injection of 100 ng of estrogen (E2, Sigma-Aldrich, #E2758) for three consecutive days ...
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bioRxiv - Evolutionary Biology 2024Quote: ... at 10 nM or for two days with 17-ß estradiol (E2) (Sigma Aldrich, E8875) at the same concentration following by two days with progesterone (P4 ...
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bioRxiv - Neuroscience 2023Quote: ... od=3.18 mm) containing either 100 μg/ml E2 (Sigma Chemical Co., St Louis, MO) in sunflower oil (OVX+E2 group ...
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bioRxiv - Neuroscience 2024Quote: Ovariectomized (OVX) females were treated with a single administration of 17β-estradiol (E2, Sigma-Aldrich cat# 3301 ...
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bioRxiv - Biochemistry 2024Quote: Human C-reactive protein (Cat. No. C4063) and Human erythrocyte catalase (Cat. No. C3556) were obtained from Sigma Aldrich chemicals ...
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bioRxiv - Biochemistry 2023Quote: ... or a VSVG epitope tag on the N-terminal tag of H2A (Sigma, V4888) and a fluorescently labelled secondary antibody (Cell Signaling ...
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bioRxiv - Cell Biology 2019Quote: ... containing 10% FCS (Sigma), 100U/ml penicillin and 100µg/ml streptomycin (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... 10% FCS (Sigma-Aldrich), 1% Penicillin-Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented 10% FCS (Sigma), 1% Pen Strep (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... 10% FCS (Sigma Aldrich), 100 units/ml penicillin ...
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bioRxiv - Immunology 2022Quote: ... 10% FCS (Sigma-Aldrich) and 50 U/ml Pen/Strep (Gibco ...
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bioRxiv - Pathology 2019Quote: ... FCS (10 %) (Sigma-Aldrich), b-FGF (10 ng/ml) ...
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bioRxiv - Immunology 2019Quote: ... and 2% FCS (Sigma) adjusted to pH 7.4 at 37°C and sterile filtered) ...
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bioRxiv - Microbiology 2020Quote: ... 2% FCS (Sigma-Aldrich), 20 mM HEPES buffer (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... 5% FCS (Sigma-Aldrich), 20 μg/ml gentamycin (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... 12.5% FCS (Sigma-Aldrich), 1% PenStrep and 100IU/ml interleukin-2 (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... Fc control (Millipore, #401104), ephrinB1-Fc (R&D ...
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bioRxiv - Immunology 2024Quote: ... 10% FCS (Sigma–Aldrich) and 1% HEPES (Gibco)] ...
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bioRxiv - Cancer Biology 2021Quote: ... Flag-tag (Sigma, F3165-.2MG), Veriblot IgG (Abcam ...
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bioRxiv - Genomics 2019Quote: ... Flag-tag M2 (F1804, Sigma), goat IgG control (sc-2028 ...
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bioRxiv - Microbiology 2019Quote: ... Flag-tag (Sigma-Aldrich, 1;1000) and β-actin (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... Anti-FLAG tag (Sigma, #F1365), anti-PGK1 (Thermo # PA5-28612) ...
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bioRxiv - Molecular Biology 2020Quote: ... HIS-tag (Sigma-Aldrich H1029), and Streptactin-HRP (ThermoFisher Scientific #21130) ...