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Citations for Millipore Sigma :
101 - 150 of 9820 citations for M CHERRY MRNA mCherry Fluorescent Protein coding mRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Microinjection of Tol2 expression constructs (25 ng/µl) and Tol2 mRNA (50 ng/µl) with 0.025% phenol red (P0290; Sigma) was performed on one-cell-stage zebrafish embryos under a dissection microscope (SMZ445 ...
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bioRxiv - Cancer Biology 2020Quote: ... The cGAS knockdown for RNA interference was achieved using Mission shRNA-encoding lentivirus directed to human cGAS mRNA (Sigma; GenBank/EMBL/DDBJ accession no ...
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bioRxiv - Microbiology 2021Quote: An shRNA lentiviral vector targeting the CDS region of PROX1 mRNA (Clone ID: NM_002763.3-531s21c1) was purchased from Sigma-Aldrich. Lentiviral particles were prepared by transfecting HEK293T cells with pLKO.1-shPROX1 (or pLKO.1-shControl) ...
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bioRxiv - Cell Biology 2022Quote: Transfer plasmids with pre-designed shRNA against human Cdc42 mRNA were obtained from the Mission® TRC library (Sigma). Specifically ...
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bioRxiv - Bioengineering 2023Quote: ... The mRNA was extracted by citrate-saturated phenol (pH 4.7): chloroform: isoamyl alcohol (125:24:1) (Sigma-Aldrich, UK). After centrifugation at 10,000x g ...
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bioRxiv - Bioengineering 2023Quote: ... the unencapsulated mRNA content (Cf) and the mRNA content (Ct) of the same sample after adding 0.1% (v/v) Triton X-100 (Sigma) were measured to calculate the mRNA encapsulation efficiency ...
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bioRxiv - Bioengineering 2023Quote: ... The encapsulated mRNA-LNPs were concentrated by changing the buffer using a centrifugal filter (Amicon Ultra, Millipore, 30 kDa) or a Tangential Flow Filtration (TFF ...
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bioRxiv - Biochemistry 2022Quote: ... and protein concentration was measured with BCA assay (Sigma 71285-M). Then ...
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bioRxiv - Immunology 2023Quote: Fluorescent dye inactivation was performed by bleaching the slides with 0.1 M NaHCO3 (pH 10.9-11.3) (Sigma, S6297) and 3% H2O2 (Merck ...
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bioRxiv - Microbiology 2023Quote: ... and 0.5 M NaCl) before eluting bound proteins with buffer A containing 0.5 M Methyl-α-D mannose (Sigma). Fractions containing NA activity were pooled ...
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bioRxiv - Microbiology 2024Quote: ... The protein pellet was washed in protein wash buffer (0.3 M guanidine hydrochloride (Sigma Aldrich, USA) in 95% ethanol ...
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bioRxiv - Microbiology 2020Quote: ... Total mRNA was extracted from 0.5 x 106 cells using a single-step RNA isolation reagent- TRI reagent (T9424, Sigma-Aldrich) at specified time points ...
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bioRxiv - Developmental Biology 2021Quote: ... Zebrafish lines expressing transgenic Spaca6 were generated by injecting the spaca6 expression construct with Tol2 mRNA into spaca6+/− zebrafish embryos (15 ng/μl of the plasmid in RNase-free water, 35 ng/μl Tol2 mRNA, 0.083% phenol red solution [Sigma-Aldrich]), following standard procedures ...
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bioRxiv - Molecular Biology 2021Quote: ... Cytoplasmic injection of Cas9 mRNA and sgRNAs (100 and 50 ng/ul, respectively) was performed in zygotes collected in M2 medium (Sigma) at E0.5 ...
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bioRxiv - Neuroscience 2021Quote: ... Injection solutions were prepared with a final concentration of 300ng/µl nCas9n mRNA and 10ng/µl sgrNA in nuclease free water and 0.05% (w/v) phenol red (Sigma Aldrich). Wildtype embryos were injected at the single or two cell stage with 1.5nL injection solution ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were hybridized overnight at 37°C with sixteen FLAP-Y-Cy-3 tagged complementary oligonucleotide probes, targeting regions in Cox7c mRNA (IDT) in Hybridization mix (15 % Formamide, 1.7 % tRNA (Sigma; R1753), 2 % FLAP:Probe mix ...
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bioRxiv - Biochemistry 2021Quote: ... SP cells lacking endogenous mRNA were resuspended (3×106 SP cells/mL as determined by trypan blue (Sigma-Aldrich, T8154) staining ...
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bioRxiv - Microbiology 2020Quote: 2 μg mRNA suspended in 6 μl RNAse-free H2O was digested with 2 units of nuclease P1 (Sigma N8630) for 3 h at 37 ºC ...
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bioRxiv - Cancer Biology 2022Quote: ... Two pLKO.1 lentiviral vectors encoding for specific shRNA for two human MAPK7 mRNA sequences were used (TRCN0000010262/pLKO.1 and TRCN0000010275/pLKO.1, Sigma). Lentiviral particles were generated in HEK-293 cells by co-transfecting the virion vectors (psPAX2 and pMD2G ...
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bioRxiv - Developmental Biology 2021Quote: ... mRNA was first isolated from 40 dpf wild-type AB strain ovaries using TRI reagent (Cat. No. T9424; Sigma-Aldrich) and then cDNAs were synthesized with RETROScript Reverse Transcription Kit (Cat ...
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bioRxiv - Molecular Biology 2022Quote: RNA used for ONT sequencing was poly(A+) selected in two consecutive rounds using oligo(dT) beads (GenElute mRNA Miniprep Kit, Sigma MRN10 ...
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bioRxiv - Biochemistry 2023Quote: ... 400 μg total RNA in 250 μl water was applied to a GeneElute mRNA miniprep kit (Sigma-Aldrich; MRN70-1KT), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... treated with LNPs containing mRNA sequences of either the anti-NSP9 nanobody or tdTomato were used for total mRNA isolation by TRI Reagent (Millipore-Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: Antisense Oligonucleotides (ASOs) directed against sequences encompassing initiator methionines and polyadenylation sites of candidate mRNAs were purchased from Sigma-Aldrich. All ASOs were 20mers ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluted M-protein fractions were collected in Eppendorf tubes prefilled with 5 μl 2 M TRIS.HCl (pH=7, Sigma Aldrich), to neutralize the solution ...
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bioRxiv - Biophysics 2023Quote: ... The samples were washed five times with extraction buffer and then immunoblotted with anti-Cherry antibodies (Sigma-Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... A mixture of primary antibodies against enhanced green fluorescent protein (EGFP: chicken, Millipore), human influenza hemagglutinin (HA ...
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bioRxiv - Plant Biology 2022Quote: ... the coding sequence for the mature STIC2 protein (aa 49-182) was cloned into BamHI/SalI linearized pETDuet1 (Novagen) and pGEX4T3 (Cytiva ...
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bioRxiv - Biophysics 2020Quote: Proteins were buffer exchanged into 1 M aqueous ammonium acetate (Sigma-Aldrich) using P6 Biospin columns (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Whole-cell protein lysates were prepared in CelLytic M lysis buffer (Sigma) with 1% protease/phosphatase inhibitors (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Cordycepin was added to the 3’ end of in vitro transcribed mRNA by substituting 50 mM Cordycepin (3’-deoxyadenosine) 5’-triphosphate sodium salt (Sigma-Aldrich) in 10 mM Tris pH7 for ATP in a poly(A)-tailing reaction with a Poly(A ...
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bioRxiv - Immunology 2020Quote: ... NINJA mice were generated by transfecting the PvuI-HF linearized NINJA targeting construct into B6 ES cells (JM8) along with mRNA encoding ZFNs for the Rosa26 locus (Millipore Sigma). We screened clones for proper targeting by southern blotting and identified one correctly targeted clone (Fig S7) ...
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bioRxiv - Neuroscience 2021Quote: Zebrafish eggs were injected at the one cell stage using a pressure microinjector with 1nl of injection solution (20-30 ng/μl plasmid DNA, 25-60 ng/μl transposase mRNA and 1% phenol-red (Sigma Aldrich)) ...
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bioRxiv - Biophysics 2020Quote: ... The stress was present for 30 minutes before induction of the reporter mRNA construct using 10 ng/mL anhydrous tetracycline (aTc, Sigma-Aldrich). The time of aTc induction marked the t=0 time point in imaging experiments ...
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bioRxiv - Developmental Biology 2019Quote: ... we extracted mRNAs with phenol-chloroform and purified them through Sephadex G-50 Quick Spin Column for radiolabeled RNA purification (Sigma-Aldrich), previously equilibrated 6 times with 1mL of TE 10mM.
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bioRxiv - Microbiology 2019Quote: Lentiviral particles carrying an anti-CTIF shRNA were produced in HEK293 cells by transfecting a commercially available pLKO.1 vector containing the shRNA sequence targeting the 3’-UTR of the CTIF mRNA (Sigma-Aldrich), pVSVg and psPax2 ...
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bioRxiv - Molecular Biology 2021Quote: ... 50–60 zebrafish embryos injected with PACE reporter mRNAs were snap-frozen at the sphere stage and lysed with lysis buffer containing cycloheximide at 100 μg/ml (Sigma-Aldrich). An S-400 HR gel filtration spin column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... resuspended in 100 ul KHM buffer and endogenous mRNA removed by treatment with 0.2 U Nuclease S7 Micrococcal nuclease from Staphylococcus aureus (Sigma-Aldrich, 10107921001) in the presence of 1 mM CaCl2 at 22°C for 12 min ...
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bioRxiv - Cell Biology 2022Quote: RNA was isolated by phenol chloroform extraction and alcohol precipitation followed by two consecutive rounds of poly-A selection using oligo(dT) beads (GenElute mRNA Miniprep Kit, Sigma MRN10) following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2024Quote: Total mRNA was extracted from larval brains of control (yw) and mrtf[Delta7] at 24 h ALH using TRI Reagent (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... mRNA was isolated and converted into cDNA using a commercially available kit (GenElute Mammalian Total RNA Miniprep Kit; Sigma-Aldrich, USA). Transcript levels were determined by real-time quantitative polymerase chain reaction (QuantStudio 5 ...
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bioRxiv - Developmental Biology 2021Quote: ... Total proteins were extracted from cells with the M-PER Mammalian Protein Extraction Reagent (Sigma, GE28-9412-79) supplemented with protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... wandering 3rd instar larvae were dissected in HL390 using a standard larval fillet dissection then fixed in 4% paraformaldehyde (or, for UAS-Atg8-Cherry experiments Bouin’s fixative (Sigma)) for 20 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were mixed with 3 μl of 2μM fluorescent primer (D4-5′-TTTCTTTCCCCCTGGCCTT for the probed/control samples and D2-5′-TTTCTTTCCCCCTGGCCTT for the sequencing reaction, Sigma) and incubated for 5 minutes at 65°C ...
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bioRxiv - Biophysics 2023Quote: The LAF-1 protein coding sequence was ordered as a gBlock Gene Fragment (TM) and inserted into a pET28a(+) vector from Novagen. The LAF-1 mutants E398A (DAAD ...
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bioRxiv - Molecular Biology 2019Quote: ... Green fluorescent protein (GFP) expression was monitored by flow cytometry (FACSCalibur Guava 8HT [Millipore]) starting on day 12 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein was extracted using 100 µL of lysis buffer (7 M urea (Sigma), 2 M thiourea (Sigma ...
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bioRxiv - Cell Biology 2021Quote: Protein was extracted from cells using lysis buffer (CelLytic™ M, Sigma-Aldrich) supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... protein was extracted from SC cells using lysis buffer (CelLyticTM M, Sigma-Aldrich) supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were solubilised in solubilising buffer containing 1 M hydroxylamine hydrochloride (159417, Sigma), 1 mM HPDP-biotin (A8008 ...