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Citations for Millipore Sigma :
1 - 50 of 9820 citations for M CHERRY MRNA mCherry Fluorescent Protein coding mRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Chromosomes were labeled by either injecting mRNA encoding human H2B-mCherry or H1 protein purified from calf thymus (Sigma) labeled with AlexaFluor 568 or 647 as described previously (Bun et al. ...
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bioRxiv - Immunology 2022Quote: ... The mRNA was purified using LiCl (Sigma, SLCC8730, 2 M final concentration). To generate the LNPs ...
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bioRxiv - Microbiology 2022Quote: Coding sequences for M proteins were cloned into a modified pET28b vector (Novagen) that contained sequences encoding an N-terminal His6-tag followed by a PreScission protease cleavage site (5) ...
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bioRxiv - Molecular Biology 2019Quote: ... Large mRNA amounts (>1μg) were prepared by GenElute mRNA miniprep kit (Sigma, MRN70). All kits procedures were conducted according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2019Quote: ... 50ng/ul Cas9 mRNA (Sigma) together with 4 sgRNAs (6.25ng/µl ...
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bioRxiv - Cell Biology 2019Quote: ... Cas9 mRNA (Sigma CAS9MRNA-1EA), single strand oligonucleotide donor template containing K778R mutation ...
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bioRxiv - Cancer Biology 2020Quote: mRNA was purified from total RNA with 2 rounds of GeneElute mRNA purification kit (Sigma). rRNA was removed using Ribominus kit (Invitrogen ...
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bioRxiv - Genetics 2023Quote: Human mCherry-tagged CTNS mRNA solutions were prepared in RNAse free water (500ng/µl) with 0.05% PhenolRed (Sigma, cat.no.P0290). All mRNA injections were performed at the one-cell stage using a FemtoJet microinjector (Thermofisher ...
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bioRxiv - Immunology 2020Quote: ... and poly(A)+ mRNA was enriched from total RNA with GenElute mRNA Miniprep Kit (Sigma-Aldrich). The cDNA was reverse transcribed using M-MLV reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... A mixtures of Cas9-mRNA (Sigma)(50ng/μL) ...
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bioRxiv - Immunology 2022Quote: ... mRNA was purified by cellulose (Sigma) purification ...
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bioRxiv - Molecular Biology 2020Quote: ... and mRNA sequence specific primers (Sigma-Aldrich). The qPCR was performed with 2 μL of a 1:10 dilution of the cDNA for the genes ACT1 ...
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bioRxiv - Neuroscience 2019Quote: ... Cas9 mRNA was obtained from Sigma-Aldrich. Circular DNA donor plasmids were purified with an endotoxin-free kit (Qiagen).
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bioRxiv - Cancer Biology 2019Quote: ... The mRNA primer designs (Sigma-Aldrich, USA) are listed in the Supplementary Table 3a ...
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bioRxiv - Bioengineering 2024Quote: ... mRNA was purified by cellulose (Sigma-Aldrich) purification as described52 ...
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bioRxiv - Developmental Biology 2023Quote: ... ripply1 mRNAs was detected using FastRed (Sigma). Whole-mount embryos were imaged using the Viventis LS1 light-sheet microscope with 3um z-slices and 3.3 um laser beams ...
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bioRxiv - Cancer Biology 2023Quote: ... For mRNA or protein decay analyses cells were treated with actinomycin D (5 µM, Sigma Aldrich) or emetin (100 µM ...
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bioRxiv - Biochemistry 2021Quote: Total RNA was isolated using the Trizol method and mRNAs were isolated with the GenElute™ mRNA Miniprep Kit (Sigma). The concentration and purity of the mRNA were measured using a NanoDrop 2000 ...
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bioRxiv - Cell Biology 2021Quote: ... Cas9 mRNA was purchased from Sigma (CAS9MRNA-1EA). The single strand donor oligonucleotide contained the double point mutation in HOIL-1L-NZF (T201A/R208A) ...
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bioRxiv - Molecular Biology 2023Quote: Probes for LacZ mRNA (Sigma-Aldrich, 03-307) were used as negative control ...
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bioRxiv - Cell Biology 2023Quote: ... sgRNA and CAS9 mRNA (CAS9MRNA-1EA, Sigma-Aldrich) were injected into the nuclear region of fertilized eggs from C57BL/6 mice with the concentration of 0.1g/L and 0.05g/L ...
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bioRxiv - Biochemistry 2021Quote: ... Methylated mRNA was eluted by free m6A from the beads and purified with the GenElute™ mRNA Miniprep Kit (MRN70, Sigma). One tenth of the fragmented RNA was saved as an input control for standardization ...
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bioRxiv - Cell Biology 2023Quote: ... treated with LNPs containing mRNA sequences of either the anti-NSP9 nanobody or tdTomato were used for total mRNA isolation by TRI Reagent (Millipore-Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... The injection mix consisted of Cas9 mRNA (Sigma Aldrich) (final concentration of 50 ng/ul ...
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bioRxiv - Molecular Biology 2019Quote: ... and GenElute™ mRNA Miniprep kit (MRN10, SIGMA-ALDRICH), respectively.
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bioRxiv - Biochemistry 2021Quote: ... mRNA was incubated with 15 units of benzonase (Millipore), 2 units of phosphodiesterase I (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: mRNA was extracted using TriReagent (Sigma-Aldrich, St. Louis) according to the manufacturer’s protocol along with glycoblue co-precipitant (1.6 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... the synthesized mRNA was isolated from the reaction mix by precipitation with half volume of 8 M LiCl solution (Sigma-Aldrich, Hamburg, Germany), and finally the pellet was dissolved in nuclease-free water ...
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bioRxiv - Developmental Biology 2021Quote: ... The injection mix (50 ng/μl Cas9 mRNA (Sigma-Aldrich), 50 ng/μl SPACA4_gRNA1 and 2 ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragments encoding shRNAs directed against human CDH13 mRNA (Sigma) were cloned into the pTRIPΔU3-EF1α-EGFP lentiviral vector ...
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bioRxiv - Molecular Biology 2022Quote: ... 200 ng mRNA were digested with 1U nuclease P1 (Sigma) in 20 mL buffer containing 25 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... Cas9 mRNA used for injections was purchased from Sigma (#CAS9MRNA). Primer sequences are provided in the Table S4.
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bioRxiv - Molecular Biology 2021Quote: ... mRNA was isolated using chloroform and isopropanol (C2432, I9516; Sigma-Aldrich). After precipitation with isopropanol ...
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bioRxiv - Genetics 2020Quote: ... 200 ng mRNA was mixed with 0.1 U Nuclease P1 (Sigma) and 2.0 U calf intestinal phosphatase (New England Biolabs) ...
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bioRxiv - Systems Biology 2023Quote: ... mRNA was released by treating the tissue with pepsin (P7000, Sigma) in 2x SSC (pH 2.5 ...
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bioRxiv - Immunology 2019Quote: ... mRNA synthesis was inhibited with 5 μg/ml actinomycin D (Sigma-Aldrich). Inhibitors targeting p38 MAPK (p38i ...
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bioRxiv - Cell Biology 2022Quote: ... mRNAs were purified using Sera-Mag oligo(dt) magnetic particles (Sigma-Aldrich). mRNAs were fragmented with Ambion mRNA fragmentation buffer and fragmented mRNAs were purified using RNeasy MinElute Kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... or with vaccine RNAs using RiboJuice™ mRNA Transfection Kit (Merck Millipore) according to the manufacturer’s instructions and incubated for 18 h ...
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bioRxiv - Cell Biology 2019Quote: ... 200 ng purified mRNAs were incubated with nuclease P1 (0.5 U, Sigma) in 25 μl reaction system containing 10 mM NH4OAc (pH = 5.3 ...
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bioRxiv - Developmental Biology 2020Quote: ... The microinjection mix consisted of Cas9 mRNA (Sigma Aldrich, St. Louis, MO) (final concentration of 40 ng/ul ...
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bioRxiv - Cancer Biology 2020Quote: ... Decapped mRNA were then digested by 1 U of Nuclease P1 (Sigma) for 2 h at 42 °C in NH4OAc buffer (10 mM ...
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bioRxiv - Molecular Biology 2022Quote: ... rRNA was separated from polyA RNA using GeneElute mRNA Miniprep kit (Sigma) and concentrated using RNA Clean and Concentrator kit (Zymo Research ...
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bioRxiv - Developmental Biology 2024Quote: ... 40 ng/ml Tol2 mRNA and 0.2 µM KCl (Sigma, P9333-500G). Overexpression of the wild-type spns1 gene was confirmed by SYBR-green using cDNA from pools of 7-15 single (Tg(UAS:spns1))and double transgenic (Tg(fli1a:Gal4 ...
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bioRxiv - Developmental Biology 2023Quote: ... whole cell mRNA was isolated from 50 embryos using TRI-Reagent (Sigma). Reverse transcription was performed on 2.5 µg of RNA using Superscript III (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: Total mRNA was purified using the GenElute Mammalian Total RNA kit (Sigma-Aldrich). After DNase I treatment ...
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bioRxiv - Molecular Biology 2019Quote: ... purified mRNA was digested into mononucleotides with nuclease P1 (Sigma-Aldrich, 2 U) in buffer containing 25 mM NaCl and 2.5 mM ZnCl2 for 2 hours at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... mRNA was isolated from the fractions by mixing with phenol:chloroform:isoamyl alcohol (Sigma-Aldrich) and then RNA was analyzed by qRT-PCR .
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bioRxiv - Molecular Biology 2020Quote: Isolation of total mRNA from cells was performed with TRI-reagent (Sigma-Aldrich), and 1 µg of extracted RNA was reverse transcribed into cDNA according to manufacturer’s instructions using the QuantiTect Reverse Transcription Kit (QIAGEN ...
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bioRxiv - Microbiology 2021Quote: ... Total mRNA was extracted following the manufacturer protocol (Sigma-Aldrich, St. Louis, MO) followed by chloroform/ ethanol precipitation cDNA was obtained using SuperScript® III First-Strand Synthesis System (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: Total mRNAs were purified using the GenElute Mammalian Total RNA kit (Sigma-Aldrich). After 1 hr of DNase I (4U ...