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Citations for Millipore Sigma :
1401 - 1450 of 10000+ citations for Cow Calcipressin 1 RCAN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... five flies of each sex and genotype were placed into a vial with 2 ml of 1 X dyed medium (2.5 % yeast, 2.5 % sucrose, 1 % agar and 1 % Brilliant Blue FCF – Sigma Aldrich, USA). After 2 h of feeding ...
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bioRxiv - Neuroscience 2019Quote: ... for the final differentiation in neural differentiating medium (NDM) (Neurobasal 1% NEAA, 1% N2 supplement and 1% P/S) supplemented with 1µM of cAMP (Sigma-Aldrich) and 10 ng/mL of BDNF ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... or α/β-tubulin (1:1 mixture) (1:100 in PBS; 2 hr at 37°C) (Sigma; T9026, T4026, USA). FITC-conjugated anti-mouse IgG (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated in 5% horse serum in 0.5% Triton-X TBS for 1 hour before overnight incubation with 1:500 anti-GFP or 48 hours 1:300 anti-UBE3A (3E5 Sigma) and 1 hr 1:500 Alexa-Fluor 488 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... two media containing either commercialized mixed bile salts (0.5 g L−1, 1:1 sodium cholate: sodium deoxycholate, cat# 48305, Sigma-Aldrich) or a primary bile salts composition (0.25 g L−1 sodium cholate and 0.25 g L−1 sodium chenodeoxycholate ...
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bioRxiv - Cell Biology 2020Quote: ... 1999), rabbit anti-HTP-1/2 (1:500) (Martinez-Perez et al., 2008) and mouse anti-FLAG (1:200) (Sigma).
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bioRxiv - Neuroscience 2020Quote: ... GFAP (1:200, guinea pig, Synaptic Systems; 1:1000, mouse directly conjugated to Cy3, Sigma-Aldrich; 1:1000, chicken, Millipore), GFP (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... GFAP (1:200, guinea pig, Synaptic Systems; 1:1000, mouse directly conjugated to Cy3, Sigma-Aldrich; 1:1000, chicken, Millipore), GFP (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with primary antibodies (rabbit anti-miniSOG 1:500 [Tsien Lab]; guinea pig anti-VGLUT2 1:2000 [Millipore #AB2251]; rabbit anti-TH 1:1000 [Millipore #AB152] ...
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bioRxiv - Biochemistry 2020Quote: ... The full-length MtaLonA were prepared by supplementing the growing medium with 1 g l−1 of 15NH4Cl and 4 g l−1 of 2H7/12C6-glucose in 99.8%-2H2O (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... anti-γH2AX (JBW301, 1:1000), anti-P-Ser15-p53 (1:1000) and anti-ATM Ser1981 (10H11.E12, 1:200) were from Millipore. Anti-P-KAP1Ser824 (1:1000) ...
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bioRxiv - Developmental Biology 2021Quote: ... and then with Theobroma cacao extract fraction 5 (100 µg.mL-1) or (-)-epicatechin (80 µg.mL-1) (Sigma-Aldrich, HW101708-1) for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in PBS/0.1% Triton X-100 and incubated with the secondary antibody (1:500) and 1 mg ml−1 DAPI (Sigma, D9564) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... a mixture of primary antibodies in PBS-XD overnight (1 μg/ml rabbit anti-GAD67/65, Frontier; 1:4000 mouse anti-glutamate, ImmunoStar; 1:500 goat anti-ChAT, Millipore), and a mixture of secondary antibodies in PBX-XD for two hours (donkey anti-goat ...
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bioRxiv - Neuroscience 2022Quote: ... 1:100 mouse anti-ASCL1, Guillemot lab; 1:800 rat anti-BrdU, Abcam ab6326; 1:800 chicken anti-GFAP, Millipore AB5541 ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was washed three times with 1 X TBST and incubated for 1 h with 1:10,000 diluted secondary antibody (Anti-Rabbit IgG peroxidase, Sigma-Aldrich) at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... 12565501) with 0.01M phosphate-buffered saline and processed immediately for zonula occludins-1 (ZO-1; 1:500, Millipore Sigma, MABT11) and Bmal1 (Cell Signaling Technologies ...
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bioRxiv - Microbiology 2021Quote: ... and transfer vector at 1:1:1 molar ratio using the X-tremeGENE 9 DNA transfection reagent (Sigma-Aldrich; # 6365787001) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... rabbit anti-phospho-eIF2α (1:1000, CST), mouse anti-eIF2α (1:1000, CST) or mouse anti-GAPDH (1:20000, Millipore) antibodies ...
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bioRxiv - Microbiology 2020Quote: ... 150 mM NaCl and 0.1% Tween 20) were performed before incubating the membrane with the anti-FLAG antibody 1:5000 for 1 h (Sigma). Next ...
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bioRxiv - Genomics 2022Quote: ... Cells are then resuspended in 10ml Sucrose/Glycerol buffer (1:1) (Sucrose Buffer: 0.3M Sucrose; 1% Triton-X (Sigma Aldrich); 10mM HEPES (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2019Quote: Transfected COS-7 cells were washed three times with ice cold PBS and scraped in buffer K (1 mM Tris-HCl, 1 mM EGTA and 1 mM MgCl2, pH 7.6) containing protease inhibitor cocktail (Sigma, # P2714). Cells were mechanically lysed by passing them 10 times with 301/2-gauge needle and small fractions were used to measure the total protein using a Coomassie protein assay (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse monoclonal anti-Tau-1 (05-838, 1:1000), mouse monoclonal anti-MAP2 (05-346, 1:500), rat monoclonal anti-tubulin (MAB1864, 1:500) from Millipore; chicken polyclonal anti-GFP (ab13970 ...
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bioRxiv - Genomics 2020Quote: ... The blocking solution was aspirated and replaced with the same solution with primary antibodies (MAP2-Ck, Abcam ab5392, 1:500; Nanog-Gt, R&D AF1997, 1:200; TRA-1-60 IgM-Ms, Millipore MAB4360 ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were then blocked with 5% normal donkey serum in 1X PBS for one hour before overnight incubation in primary antibodies (Foxf1, goat, R&D, 1:300; Sox9, mouse, Invitrogen, 1:200; Sox9, rabbit, Millipore, 1:200; Acta2, mouse, Sigma, 1:800 ...
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bioRxiv - Plant Biology 2021Quote: ... 10%glycerol, 1 mM DTT, 1 mM EDTA, 0.5% Triton X-100 and 1% [v/v] protease inhibitor cocktail; Sigma-Aldrich). Insoluble debris was pelleted by centrifugation for 20 min with 4000 rpm at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Non-infected and Lm-infected HUVEC were incubated for 1 h with primary antibody rabbit anti-STAB-1 (1:100, Millipore), diluted in 0.2% saponin (Merck ...
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bioRxiv - Cancer Biology 2019Quote: ... This wash step was repeated 3 times and the chloroform fraction was finally dried befor e being redissolved in 200 ml of 50% methanol prior to be loaded into a conditioned (1 CV methanol, 1 CV MiliQ water, 1 CV acetonitrile (Sigma) and 1 CV Mili-Q Water ...
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bioRxiv - Molecular Biology 2019Quote: ... and SmB(residues 1-91) [pCDFDuet-HT-B(1-91)-D3(1-75)] were constructed in a single pCDFDuet vector (Novagen) with N-terminal His(6)-tag followed by Tobacco Etch Virus (TEV ...
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bioRxiv - Genomics 2021Quote: ... The beads were washed two times with high-salt wash (50 mM Tris-HCl, pH 7.4, 1 M NaCl, 1 mM EDTA, 1% Igepal CA-630 (Sigma I8896), 0.1% SDS ...
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bioRxiv - Physiology 2021Quote: ... 0.5% Na-deoxycholate and 1% Triton X100) containing protease inhibitors and phosphatase inhibitor cocktail 1 and 2 (1:100, Sigma). Protein lysates were separated on a 4-15% TGX gel (Biorad ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse monoclonal anti-Na+/K+ ATPase (1:500; SantaCruz) and anti-acetylated tubulin (1:2,000; clone 6-11B-1, Sigma Aldrich). Goat anti-mouse or rabbit IgG conjugated with HRP were used for secondary antibodies (1:10,000 ...
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bioRxiv - Cell Biology 2020Quote: ... and cultured with RPMI 1640 plus GlutaMAX + 1% (v/v) PS + 1% (v/v) B-27 + 1 μM thyroid hormone (T3) (Cat. No. T6397, Sigma) + 10 μM ALK5 inhibitor + 10 μM zinc sulfate (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... the retinas were dissected and rinsed several times with 1× PBS and incubated for 15 minutes at 1:1000 dilution of PBS with 1 mg/ml Nile Red (Sigma).
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bioRxiv - Neuroscience 2021Quote: ... Cells were blocked with 5 % BSA/PBS for 1 h before overnight incubation with primary antibodies in 1 % BSA/PBS (RAB5 21435 1:200 Cell Signalling and anti-Integrin-β1 MAB1997 1:1000, Millipore) at 4°C prior to washing and incubation with secondary antibodies (anti-rabbit AlexaFluor-546 [A11-35] and anti-mouse AlexaFluor-633 [A21052] Thermofisher ...
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bioRxiv - Developmental Biology 2020Quote: ... buffered to pH 8.3 with 1 M bicine) supplemented with 1 g/l of and Antibiotic antimycotic solution (1:100, Sigma, A5955). To dissociate into single cells ...
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bioRxiv - Genetics 2021Quote: ... Samples were then digested for 15min at room temperature using an enzymatic solution (1% driselase, 0.5% cellulase, 1% pectolyase, 1% BSA, all from Sigma-Aldrich), and washed 3 times in PBS ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of biotinylated secondary antibody diluted in PBS-BN (anti-human IgG, B1140, 1/1250; anti-human IgM, B1265, 1/1000; or anti-human IgA, SAB3701227, 1/500; Sigma) were added to all wells and incubated for 45 min at 600 rpm at RT and protected from light ...
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bioRxiv - Immunology 2021Quote: ... Positive transformants were selected on LB medium (1% tryptone, 0.5% yeast extract, 1% NaCl; Oxoid) supplemented with ampicillin (100 µg mL−1, Sigma Aldrich) for 24h at 37°C ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... Samples were dehydrated with methanol series (50%, 75%, 95%, 100%, 100%) and overnight incubated in 1:1 methanol : BABB (BenzylAlcohol-BenzylBenzoate 1:2, both Sigma)) ...
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bioRxiv - Molecular Biology 2021Quote: ... infected in BGM containing Adeno-GFP (1×1010) or Adeno-ADH5 (1×1012) and differentiated in BGM containing 0.25nM 3-Isobutyl-1-methylxanthine (IBMX, Sigma, I5879), 1uM Rosiglitazone (Sigma ...
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bioRxiv - Immunology 2020Quote: ... 50 μL of each culture supernatant were mixed with 50 μL of Griess reagent [1:1 (v/v) 0,1% N-(1-naphthyl) ethylenediamine dihydrochloride (NEED, Sigma-Aldrich) in water ...
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bioRxiv - Molecular Biology 2022Quote: ... mouse anti-γ-H2AX JBW301 (1:1000 for IB, 1:2500 for IF, 1:5000 for FACS; Millipore, 05-636), mouse anti-γ-H2AX-Alexa Fluor 647 (1:1000 for FACS ...
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bioRxiv - Biochemistry 2022Quote: ... 50 μg ml-1 spectinomycin (Sp) or/and 35 μg ml-1 chloramphenicol (Cm) or 100 μg·ml-1 ampicillin (Sigma, Merk).
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bioRxiv - Bioengineering 2022Quote: ... was assembled around the region containing photopatterned ssDNA features and wells were incubated in 10-20 μg ml-1 bovine serum fibronectin (1 mg ml-1, F1141, Sigma) diluted in sterile 50 mM HEPES (pH 8.5 ...
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bioRxiv - Bioengineering 2022Quote: ... ionised calcium-binding adapter molecule 1 (IBA1; 1:500, 019-19741, Wako) and neural nuclei (NeuN; 1:500, ABN90P, Sigma). The day after ...
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bioRxiv - Microbiology 2022Quote: ... and incubated with energy buffer (1 mM ATP, 1 mM UTP, 1 mM MgCl2, 10 mM creatine phosphate (Sigma 2380), 1 mg/mL creatine phosphokinase (Sigma 2384) ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000; NeuN, guinea pig, Millipore, 1:2000; Sox9, rabbit, Millipore, 1:1000 ...
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bioRxiv - Microbiology 2022Quote: HeLa cells were seeded in 24-well plates (1 x 105 cells x ml-1) and incubated for 24 h before 8 µg x ml-1 Polybrene (Sigma) was added to the cells ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were incubated overnight in primary antibodies targeting LC3 (1:2000) and p62 (1:2000) and aSyn (1:500) diluted in blocking solution with 0.1% Tween-20 (Sigma-Aldrich). The next day ...