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Citations for Millipore Sigma :
1351 - 1400 of 10000+ citations for Cow Calcipressin 1 RCAN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... γ-tubulin (Ms GTU-88, Sigma, WB 1:1000, IF 1:500), GAPDH (Ms ...
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bioRxiv - Neuroscience 2024Quote: ... acetylated tubulin (1:10000, clone 6-11B-1, T6793, Sigma-Aldrich) or α-tubulin (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Na+/K+ ATPase α-1 (Sigma-Aldrich, 05-369; 1:200), GFAP (Millipore ...
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bioRxiv - Bioengineering 2024Quote: ... 1 μg mL-1 concanavalin A (ConA, Sigma-Aldrich, C0412-5MG) as a positive control ...
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bioRxiv - Neuroscience 2024Quote: ... total Rab12 (1:1000 Proteintech and β-actin (1:3000, Sigma) at 4C overnight ...
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bioRxiv - Physiology 2024Quote: ... GAPDH (CST14C10#2118; 1:5000) and Vinculin (Sigma #V9131; 1:5000) (as described in Table S3) ...
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bioRxiv - Cell Biology 2024Quote: ... anti-pTyr357-YAP (Sigma, Y4645; IB 1:1000, IF 1:100); anti-p73 (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2024Quote: ... mouse anti-FLAG M2 (1:5,000 or 1:10,000, Sigma, F1804), rabbit anti-GFP (1:2,500 ...
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bioRxiv - Developmental Biology 2024Quote: ... mixed 1:1 with pre-chilled (−20°C) isopropanol (100%, Sigma), and incubated at −20°C overnight (∼16 hours) ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were rinsed with TBS and blocked with TBS with 5% non-fat milk for 1 hour at room temperature and then incubated with mouse monoclonal antibody for Muc5b (1:500 vol:vol) or lectin UEA-1 L8146 1mg/mL (1:1000 vol:vol) (Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-α-tubulin (1:200 for IF and 1:2500 or 1:10,000 for IB; Sigma-Aldrich); rabbit polyclonal anti-CALK (1:10000 ...
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bioRxiv - Neuroscience 2021Quote: ... Paclitaxel was prepared in 1 part vehicle (1:1 v/v Cremophor EL [EMD Millipore] and 200- proof ethanol) and 2 parts sterile saline (UPS) ...
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bioRxiv - Cancer Biology 2021Quote: ... Necrostatin-1 (N9037-10NMG), Ferrostatin-1 (SML0583-5MG) and 1-Fluoro-2,4-dinitrobenzene (DNFB, D1529) were purchased from Sigma. HB072 was obtained as previously shown (23).
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bioRxiv - Microbiology 2021Quote: ... neoformans cells were added to washed macrophage monolayers at a 1:1 ratio with 10 units (1 unit = 0.1 ng/mL) of recombinant murine IFNγ (Sigma) and 0.3 μg/ml of lipopolysaccharide (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary (1:100) and secondary (1:200) antibodies were diluted in PBS containing 1% (v/v) BSA (Sigma-Aldrich). Primary antibodies were incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... permeabilized in 1% DMSO/1% Triton X-100 and stained with anti-phospho H3 antibody (1:250, Merck Millipore) followed by anti-rabbit Alexa 488 secondary antibody (1:1000 ...
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bioRxiv - Systems Biology 2022Quote: ... Cultures were treated with the indicated amount of 1-(1,1-dimethylethyl)-3-(1-naphthalenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (1-NM-PP1; Millipore Sigma), taken from a 10,000x stock in DMSO ...
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bioRxiv - Genetics 2021Quote: ... were resuspended in 4 volumes lysis buffer (50 mM Tris-HCl pH 7.5, 1 mM dithiothreitol, 1 mM ethylenediaminetetraacetic acid, 1:400 Sigma protease inhibitory cocktail P8340 ...
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bioRxiv - Neuroscience 2023Quote: ... or PV antibody (1:500, Cell Signaling D3S8R; SST: 1:100, Millipore MA5-16987; PV: 1:1000, Swant GP72) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... pH 10.4, 1 mM MgCl2, 1 mM ZnCl2 containing 1 mg/mL 4-nitrophenyl phosphate disodium salt hexahydrate, Sigma) were added to 20 μL of the conditioned medium ...
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bioRxiv - Cell Biology 2023Quote: ... and lysis buffer (1% Triton-X, 10 nM NEM, 1 mM PMSF, 1 x Protease Inhibitor Cocktail, Sigma-Aldrich) was added ...
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bioRxiv - Neuroscience 2023Quote: ... pH 10.4, 1 mM MgCl2, 1 mM ZnCl2 containing 1 mg/mL 4-nitrophenyl phosphate disodium salt hexahydrate, Sigma) were added to 20 μL of the conditioned medium ...
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bioRxiv - Biochemistry 2023Quote: ... 1:2500, anti-Ubiquitin P4D1, Biolegend, 1:10,000, anti-UBR4/p600 ab86738, Abcam, 1:5000, anti-FLAG M2, Sigma F1804 ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 1.25 g mL−1 of lyophilized lysozyme and 1 U mL−1 of benzonase nuclease (Merck Millipore), and lysed with an EmulsiFlex-B15 cell disruptor (Avestin) ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated on ice for 3 min and 2 mL wash buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.1 % Tween-20, 1 % BSA, 1 mM DTT, 1 U/µL Sigma Protector RNase inhibitor in nuclease free water ...
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bioRxiv - Neuroscience 2023Quote: ... CaBP (1:2000, rabbit, CB38, Swant or 1:2000, mouse, #300) and VGLUT2 (1:7000, guinea pig, AB2251, Millipore). The slices were washed 3 times in 1% Triton X-100 in PBS for 5 to 10 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... 1:200 dilution,36 anti-Yb (1G2H3, 1:200 dilution),56 anti-Flag M2 (F3165, 1:1,000 dilution, Sigma) monoclonal and anti-c-Myc (1:300 dilution ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 0.5% NP-40, 50 mM NaF, 1 mM NaVO3, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride; all from Sigma). Total protein was separated on 10% SDS-PAGE reducing gels or 4%-20% detergent gels (BioRad) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were incubated with primary antibodies overnight at 4C (DARPP-32 1:200 Abcam ab40801; FoxP1 1:500 Abcam ab16645; GAD65/67 1:500 Millipore AB1511; MAP-2 1:1000 Millipore MAB3418) and then washed three times with PBS before an overnight 4C incubation in secondary antibody (Alexa-fluor rabbit 555 IgG ...
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bioRxiv - Immunology 2022Quote: ... the total DNA methylation signature was evaluated in 100 ng of DNA using the commercial kit (Imprint Methylated DNA Quantification kit, Sigma-Aldrich), according to the manufacturer’s recommendations.
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bioRxiv - Bioengineering 2020Quote: ... Apoptosis was assessed by DNA fragmentation staining using a commercially available kit (In Situ Cell Death Detection Kit, TMR red, Sigma-Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... revelation was performed using an avidin/biotin complex coupled with peroxidase (ABC kit, Universal Vectastain Kit PK6200) and diaminobenzidine (Sigma Aldrich).
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bioRxiv - Neuroscience 2020Quote: TUNEL was performed using a standard kit according to the manufacturer’s protocol (TMR In Situ Cell Death Detection Kit, Sigma-Aldrich, #12156792910) in combination with nuclear staining using DAPI (10 ug/mL ...
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bioRxiv - Microbiology 2020Quote: For the measurement of proteasome activity a fluorometric assay kit (Proteasome 20S Activity Assay kit) that measures the chymotrypsin-like protease activity was used following manufactured instructions (Sigma-Aldrich). A total of 100 µg of protein extracts were incubated with LLVY-R110 substrate provided by the kit for 2 h at 30°C in dark ...
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bioRxiv - Microbiology 2022Quote: Aldehyde and D-lactate production in Salmonella strains were measured by the Aldehyde Assay Kit and D-Lactate Assay Kit (Sigma-Aldrich), respectively ...
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bioRxiv - Synthetic Biology 2020Quote: ... the intracellular ATP were measured using the enzymatic assay kit (ATP Bioluminescence Asssay Kit HS II, Sigma No. 11699709001, Roche, Switzerland) after the cell disruption of the corynebacterial strains ...
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bioRxiv - Zoology 2020Quote: ... DNA was extracted from individual cysts or juveniles using the “Sigma Extract-N-Amp Kit (XNAT2)” kit (Sigma, St. Louis, MO) according to the protocol reported by Ma et al ...
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bioRxiv - Microbiology 2022Quote: ... Cells were verified using the Promega GenePrint STR kit (DNA Analysis Facility, Duke University) and as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Evolutionary Biology 2024Quote: Random-PCR amplification of the Viral DNA or RNA were respectively achieved with the GenomePlex Complete Whole Genome Amplification (WGA) Kit and the TransPlex Whole Transcriptome Amplification (WTA) Kit (Sigma-Aldrich) according the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... mRNA was isolated and converted into cDNA using a commercially available kit (GenElute Mammalian Total RNA Miniprep Kit; Sigma-Aldrich, USA). Transcript levels were determined by real-time quantitative polymerase chain reaction (QuantStudio 5 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cell proliferation was determined with the iClick EdU Imaging Kit (ABP Biosciences) and apoptosis was measured using the ApopTag Peroxidase in Situ Apoptosis Detection Kit (Millipore Sigma), following the manufacturers’ protocols ...
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bioRxiv - Neuroscience 2023Quote: ... 2 x 100% for 30 minutes each) and then embedded in epoxy embedding medium kit (Epon substitute embedding medium kit; 45359, Sigma Aldrich). For this ...
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bioRxiv - Neuroscience 2023Quote: ... All cells were regularly tested for the presence of mycoplasma using the PCR Mycoplasm Test Kit (PromoKine) or the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Genetics 2021Quote: ... all diluted in a 1:1 mixture of PBT and 3% BSA: mouse anti-α-tubulin (1:500, Sigma T6199), rabbit anti-Spd-2 (1:4.000 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1% ITS-X supplement (WAKO) and purified BSA (3%, WAKO)) and N2B27 (DMEM/F12/GlutaMax + Neurobasal at 1 : 1 (Sigma), 1 × B27 (Gibco) ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were incubated with the following antibodies against phospho-Yap at s112 (1:1000, 13008, cell signaling), Yap (1:2000, NB110-58358, Novus) and Rabbit anti GAPDH (1:8000, PLA0125, Sigma) for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10, 1:50, Roche, or rabbit anti-FLAG, 1:1000, SIGMA) followed by incubation with Alexa488- and/or Alexa647-coupled secondary antibodies (Molecular Probes ...
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bioRxiv - Molecular Biology 2020Quote: ... and then modified with 1:1 volume ratio with 1 mg/ml biotin-5-fluorescein conjugate (Sigma-Aldrich, cat #53608) followed by three washes with PBS ...
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bioRxiv - Bioengineering 2022Quote: ... 1 g L−1 (NH4)2-H-citrate and 1 mL antifoam (Antifoam 204, Sigma-Aldrich, St. Louis, MO, USA). The medium was supplemented with with 2 mL L−1 trace elements solution and (1 M ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell Signalling Technology), ERK (1: 1000, Cell Signalling Technology), p-ERK (1: 1000, Cell Signalling Technology) and GAPDH (1: 5000, Sigma) overnight at 4°C ...