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Citations for Millipore Sigma :
1401 - 1450 of 8159 citations for CEA Calibrator Grade Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Protein was transferred to PVDF membrane (Millipore) using a Tris/glycine transfer buffer with 20% methanol ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to PVDF membranes (Millipore), blocked with 5% milk in TBS/0.1% Tween-20 (TBST ...
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bioRxiv - Biochemistry 2023Quote: Ultra centrifugal filters for protein concentration (Millipore-Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... 20 μL of protein G Sepharose (SIGMA), previously blocked for 1 hr with lysis buffer containing 1% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... Protein G agarose beads (Millipore # 16-201) were used to pull down the antibody-protein complex ...
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bioRxiv - Cancer Biology 2023Quote: ... and horse myoglobin as standard proteins (Sigma). All spectra were processed and analyzed using MassLynx 4.1 software.
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bioRxiv - Evolutionary Biology 2023Quote: ... 50 µl of protein A-sepharose (Sigma) were added to each sample ...
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bioRxiv - Microbiology 2023Quote: ... Protein was transferred to PVDF membranes (Millipore) using the TransBlot Turbo semi-dry apparatus (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... Protein G agarose (Millipore, Temecula, CA, USA) and anti-myc antibody (Abmart ...
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bioRxiv - Microbiology 2023Quote: ... conjugated with protein G beads (Sigma-Aldrich) for 16 hours at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... 200 nM Cas9 protein (E120020-250ug, Sigma), and 500 nM circular donor plasmid were mixed in the injection buffer (10 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... protein G- sepharose beads (Sigma, Ref. P3296), GFP-Trap MA beads (Chromotek ...
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bioRxiv - Biochemistry 2024Quote: ... A Lowry assay (Sigma - total protein kit) was used to determine protein concentrations before resolving samples on an SDS-PAGE gel and transferring to nitrocellulose membrane for immunoblotting ...
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bioRxiv - Microbiology 2023Quote: ... or control protein OVA albumin (Sigma/A5503) were mixed with NL63 virus at MOI 0.1 (1:1 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred to PVDF membrane (Millipore) and blocked in 5% bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2023Quote: ... PureProteome Protein A magnetic beads (30µL; Millipore) for 2 hr at 7 rpm at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... protein expression in E.coli Rosetta cells (Novagen) was induced with 0.5 mM IPTG (isopropyl-β-D-thiogalactopyranoside ...
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bioRxiv - Plant Biology 2023Quote: ... After electroblotting proteins on nitrocellulose membrane (Millipore), protein blot analysis was performed using antiserum with specific antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 μL of protein G Sepharose (Sigma) with Casp-8 (C20 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto PVDF membranes (Millipore). Membranes were blocked in 5 % protease-free BSA (Sigma Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated in an Amicon (Millipore) 15 mL centrifugal concentrator 3K MWCO to 4-5 mL ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated in an Amicon (Millipore) 15 mL centrifugal concentrator 30K MWCO to 4-5 mL ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were transferred to PVDF (Millipore, IPVH00010) or Nitrocellulose (Sigma_Aldrich ...
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bioRxiv - Genomics 2019Quote: ... RNA-protein complexes were incubated with antibody-bound Protein A Dynabeads (anti-FLAG [Sigma Aldrich F3165] or anti-IgG) at 4C for 2 hours ...
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bioRxiv - Neuroscience 2019Quote: ... Total protein staining solution (REVERT Total Protein Stain kit, LI-COR Inc., # 926-11010; Pyronin Y (Sigma-Aldrich # P9172)
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bioRxiv - Cancer Biology 2019Quote: ... A preclearing step was made for 2hours at 4°C with 50µl of previously blocked protein-A and protein-G beads (Sigma) for 200µg of chromatin ...
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bioRxiv - Cancer Biology 2021Quote: Protein extracts were separated from each cell line with RIPA protein lysis buffer (R0278, Sigma, St Louis, MO, USA) containing 1X protease cocktail (P8340 ...
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bioRxiv - Cell Biology 2021Quote: ... The identical cell lysate was then used for protein concentration determination using Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of protein from the different S1 fractions was first treated with PureProteome Protein G Magnetics Beads (Millipore) for contaminant IgG depletion and then directly applied to dry nitrocellulose ...
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bioRxiv - Biochemistry 2022Quote: ... protein induction was performed on a small scale (2mL) and cells were disrupted using BugBuster protein extraction reagent (Novagen).
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bioRxiv - Microbiology 2021Quote: ... The cell-free supernatants were stored at –80°C for total protein quantification using the BCA protein assay (Sigma).
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bioRxiv - Plant Biology 2021Quote: ... and immunoprecipitation of the antibody/protein complexes was performed by adding 50 μl of Protein A-Sepharose (Sigma-Aldrich) made up as a 50% (w/v ...
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bioRxiv - Developmental Biology 2019Quote: ... The HDAC6 protein was immunoprecipitated from total protein lysates (0.25 mg) of cells treated with different compounds using protein A agarose beads (Sigma). The HDAC6 protein bound beads were used as enzyme source and was incubated with the HDAC assay buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... and protein diluted to 1mg/ml 1ml of protein lysate was precleared with 20μl of protein G sepharose bead slurry (Sigma) for 1 hour to remove native immunoglobulins ...
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bioRxiv - Cancer Biology 2019Quote: ... and the supernatant was tested for total protein estimate using Total Protein kit from Millipore Sigma (see material section) and cell lysate was frozen at −70°C for electrophoresis where 10µg or 20 µg per sample in sample buffer was heated up at 95°C for 5 min and run in Tris-Glycine gel followed by dry transfer for Western blot detection following the instruction for using iBlot® and/or iBlot®2 Dry Blotting with Blot®2 NC Regular Stacks.
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bioRxiv - Genomics 2021Quote: ... were captured using 40 μl of protein-A or protein-G Dynabeads (Invitrogen; pre-washed with 0.1% BSA [Fraction V, Sigma), by 4 hrs incubation time at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: Protein-protein interaction was measured by PLA with Duolink In Situ Orange Starter Kit Mouse/Rabbit (DUO92102, Millipore Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was collected and protein was estimated using the bicinchoninic acid protein estimation method (BCA, B9643, Sigma-Aldrich). We separated a protein sample (80µg ...
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bioRxiv - Paleontology 2023Quote: Information for the protein standards purchased for the ToF-SIMS analyses are as follows: type-1 collagen protein (Sigma, CAS ...
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bioRxiv - Cell Biology 2024Quote: ... Yeast protein extracts were separated on protein gels containing 10% polyacrylamide and transferred to an Immobilon-P membrane (Millipore). The membranes were probed with anti-Myc antibodies (9E10 ...
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bioRxiv - Biochemistry 2019Quote: ... 300 µl of 10% methanol (LC-MS grade) spiked with stable isotope-labelled amino acids (L-amino acid mix, Sigma-Aldrich, Co., St. Louis, MO, USA) was added to each sample ...
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bioRxiv - Biochemistry 2021Quote: ... transferrin from human serum, (Tf), cytosine (Cyt), trifluoroacetic acid (TFA, ≥99%), and formic acid (FA, analytical grade >98%) were purchased from Sigma Aldrich (St. Louis, Missouri, United States). Methanol (MeOH) ...
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bioRxiv - Cell Biology 2020Quote: ... For visualization of blue protein bands from the prestained protein ladder the anti-BLUE antibody (Anti-BLUE, 2D2-F11, Millipore) was used (Schuchner et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Fractions containing dCas9 protein were pooled and the protein was concentrated with spin concentrators (Amicon Ultra 15, MWCO 10k; Millipore), and after adding glycerol to 20% final concentration ...
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bioRxiv - Cell Biology 2020Quote: ... protein concentration was routinely determined by densitometry of Sypro-Orange stained gels against a calibration curve with protein standards (Sigma) using ImageJ ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated using centrifugal filters and concentrations were measured using the Bicinchoninic acid (BCA) protein assay kit (Millipore Sigma). Proteins were aliquoted and stored at −80°C.
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bioRxiv - Developmental Biology 2020Quote: ... Fifty or thirty-five (depending on the protein detection) micrograms of proteins was separated on 9% Tricine gels and transferred to polyvinylidene difluoride membranes (Merck Millipore). The membranes were incubated separately with anti-Mmp-9 (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein G and Protein A Dynabeads (1:1 mixed) were blocked with 1mg/ml denatured yeast tRNA (Sigma-Aldrich, R5636) and 1mg/ml of BSA (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein extracts were then mixed with the indicated primary antibodies and protein A/G agarose (Sigma, St. Louis, MO, USA) or anti-FLAG M2 agarose for overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... For the ChIP using SV40 chromatin isolated 30 minutes post-infection we substituted a mixture of protein A and protein G magnetic beads (16-663, Millipore) for the protein A and protein G agarose which we previously used in our publications ...