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Citations for Millipore Sigma :
1251 - 1300 of 8159 citations for CEA Calibrator Grade Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... The calcium-free ringer solution was replaced with 1 mL of protease solution (72 μg/mL Liberase DH research grade from Merck/Sigma, 0.4U/mL DNaseI-Invitrogen). The embryos were incubated at 28.5C on an orbital shaker for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... the cells were inoculated with 1.77*105 genome equivalents per well of HAdV-C2-dE3B-GFP in 10 μl of full medium using bovine serum albumin (BSA, cell-culture grade, Sigma-Aldrich, St. Louis, USA)-blocked small bore tubing cartridges ...
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bioRxiv - Physiology 2024Quote: Coarsely chopped liver fragments from each mouse were digested in 5 ml liberase-based digestion mix (5 ml of DMEM [Thermo Fisher Scientific, Cat #61965059] + 1 mg Liberase™ Research Grade [Roche, Cat #5401127001] + 0.1 μg DNAse I [Sigma-Aldrich, Cat #10104159001]).
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bioRxiv - Systems Biology 2023Quote: The testosterone regimen was based on our lab’s previous small animal models as well as work conducted by Kothmann et al.11,17 Testosterone cypionate (West-Ward Pharmaceuticals Eatontown, NJ, USA) 200mg/ml was diluted with pharmaceutical-grade sesame oil (Sigma-Aldrich, St. Louis, MO, USA) to reach a final concentration of 100mg/ml ...
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bioRxiv - Physiology 2023Quote: ... All solvents for lipidomic extraction and analysis used were either HPLC or LC/MS grade and purchased from Sigma-Aldrich (St Louis, MO, USA). Splash Lipidomix standards were purchased from Avanti (Alabaster ...
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bioRxiv - Plant Biology 2023Quote: ... addition of 10 μL of 20 mg ml−1 methoxyamine hydrochloride (Supelco, Catalog Number 33045-U) in anhydrous derivatization-grade pyridine (Sigma-Aldrich, Catalog Number 270970) and incubation at 37°C for 90 min with agitation at 750 RPM ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were washed three times with PBS and cell metabolism was quenched by addition of 500 µL ice-cold methanol (Fisher Chemical, 10653963) and 500 µL MS-grade water (Millipore, Direct Water Purification System). Lysates were scraped and transferred to tubes followed by the addition of 500 µL chloroform ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore, Darmstadt ...
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bioRxiv - Neuroscience 2021Quote: ... The protein concentration was then determined using the Bradford protein concentration assay (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Genomics 2020Quote: ... of GAPDH (cytoplasmic protein) and Histone H3 (nuclear protein) using rabbit anti-GAPDH antibody (1:5000, Sigma) and rabbit anti-histone H3 antibody (1:10000 ...
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bioRxiv - Microbiology 2019Quote: Quantified protein (between 80-160 μg) was incubated with protein-specific antibodies (either R anti-HA (Sigma) or anti-NS4A ...
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bioRxiv - Biochemistry 2021Quote: Protein concentrations were measured with the Bio-Rad Protein Assay Kit using bovine serum albumin (Sigma-Aldrich) as reference.
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bioRxiv - Cell Biology 2021Quote: The protein-protein interactions in fixed HeLa cells were assessed using in situ PLA (Duolink®SIGMA) according with the manufacturer’s instructions ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: Recombinant S-tagged TopoII-CTD proteins were loaded on S-protein Agarose beads (#69704, EMD Millipore/ Novagen) by incubating them together overnight ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: Recombinant S-tagged TopoII-CTD proteins were loaded on S-protein Agarose beads (#69704, EMD Millipore/ Novagen) by incubating them together overnight ...
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bioRxiv - Cell Biology 2022Quote: ... BCA was performed on the freshly isolated proteins using a Pierce BCA protein assay kit (Sigma, 23227) to determine the protein concentration ...
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bioRxiv - Cell Biology 2022Quote: The protein-protein interactions in fixed HeLa cells were assessed using in situ PLA (Duolink®SIGMA) according with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: PTP-tagged protein expression was demonstrated by immunoblotting with a rabbit polyclonal anti-protein A antibody (SIGMA) as described previously [13] ...
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bioRxiv - Systems Biology 2022Quote: ... The dried pellet was used for protein analysis using Bicinchoninic Acid Kit for Protein Determination (Sigma Aldrich).
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bioRxiv - Cell Biology 2020Quote: ... The purified protein was separated by SDS-PAGE and protein was excised after InstantBlue™ (Sigma-Aldrich) staining ...
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bioRxiv - Immunology 2019Quote: ... Protein concentration was determined using BCA protein assay reagent (Pierce Chemical Co.) with bovine serum albumin (Sigma) as the standard.
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bioRxiv - Cancer Biology 2020Quote: ... cell lysate containing about 500 μg protein was cleared with 30 μl Protein A (Millipore, Billerica, MA) for 4 h ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... 50mM HEPES pH6.6-8.5 depending on protein isoelectric point) containing complete EDTA-free protein inhibitors (Sigma-Aldrich), centrifuged for 10 min at 1,620 rcf at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Protein estimation was done on the depleted plasma protein samples using the Bradford assay (Sigma-Aldrich, USA). In the SWATH-MS analysis following steps like reduction ...
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bioRxiv - Biochemistry 2021Quote: Protein-protein interactions were analyzed using Duolink in situ orange starter fluorescence kit (mouse/rabbit, Sigma-Aldrich) in human osteosarcoma U2OS cells (WT ...
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bioRxiv - Microbiology 2020Quote: ... total protein concentration was measured in triplicates by Total Protein kit (Sigma-Aldrich, St. Luis, MO, US) using bovine serum albumin (BSA ...
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bioRxiv - Immunology 2020Quote: Mice were immunized intradermally (i.d.) in the ear with 10μg OVA protein or KLH protein (Sigma Aldritch) emulsified in complete Freund’s adjuvant (CFA ...
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bioRxiv - Cell Biology 2022Quote: ... or Protein G Sepharose beads (Protein G Sepharose® 4 Fast Flow, Sigma-Aldrich, GE17-0618-01) used for anti-Ollas antibody IPs ...
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bioRxiv - Immunology 2022Quote: ... with protein concentration determined by Coomassie G-250 Bradford protein assay kit following manufacturer’s instructions (Sigma, UK).
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bioRxiv - Plant Biology 2022Quote: Protein phosphorylation was performed by incubating 1 µg of PpeAld6PRase or myelin binding protein (MBP; Sigma-Aldrich) with the corresponding protein kinase in a standard medium containing 20 mM Tris-HCl pH 7.2 ...
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bioRxiv - Molecular Biology 2022Quote: FLC-Venus protein stability was assayed with the de novo protein synthesis inhibitor cycloheximide (C1988, Sigma-Aldrich) following the procedures in (Zhao ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was collected and total protein content determined with a BCA Protein Assay (Sigma, Poole, UK). A total of 20μg protein extract for each sample was separated by electrophoresis on Nu-PAGE™ 4-12% ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was retrieved and protein concentration was measured by BCA protein assay (Novagen, (Smith et al. 1985). Protein concentrations were adjusted in all the samples to 800 µg/ml ...
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bioRxiv - Biochemistry 2022Quote: Protein—protein interactions were studied using a Duolink In Situ Orange Starter Kit Mouse/Rabbit (Sigma, DUO92102) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: Proteins were extracted from the conditioned medium using the protein precipitation kit (#2100, Millipore Sigma, Burlington, MA) following the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2022Quote: Protein-protein interactions were studied using a Duolink In Situ Orange Starter Kit Mouse/Rabbit (Sigma DUO92102) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... All biochemical analyses were conducted using ground seed powder and all chemicals listed hereafter were of analytical grade and purchased from Sigma-Aldrich (St. Louis, MO, USA), unless otherwise specified ...
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bioRxiv - Neuroscience 2019Quote: ... that was fragmented and purified as described above was supplemented with 3 μl of formamide (biochemistry grade; Wako Pure Chemical Industries, Osaka, Japan) and overlaid with 80 μl of mineral oil (Sigma-Aldrich, St. Louis, MO, USA). DNA was completely denatured by incubating the tube at 99°C for 10 min ...
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bioRxiv - Systems Biology 2019Quote: ... Tryptic digestion was performed overnight at 37 °C after addition of trypsin solution containing 13 ng/µl proteomics grade porcine trypsin (Sigma Aldrich, St Louis, MO, USA), 100 mM ammonium bicarbonate ...
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bioRxiv - Microbiology 2021Quote: ... β-nicotinamide adenine dinucleotide phosphate (NADP+) reductive form and other required chemicals in this study were of analytical grade and purchased from Sigma-Aldrich (St. Louis, MO, USA). L-ASA was synthesized by the method of Suzanne L ...
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bioRxiv - Microbiology 2022Quote: CBB-stained bands corresponding to the bands detected by the anti-G50 antiserum were excised from the gel and treated by in-gel digestion with a proteomics-grade trypsin (Sigma-Aldrich, St Louis, MO, USA). The digested samples were purified by using ZipTip C18 (Merck) ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μL of the sample was added to a mixture containing 980 μL of pyruvate phosphate buffer (100 mM sodium phosphate, 2 mM sodium pyruvate; pH 6.0) and 100 µM NADH (Sigma-Aldrich NADH; disodium Salt, grade II) and the enzyme kinetics was measured ...
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bioRxiv - Plant Biology 2020Quote: ... The protein amount of the resuspended membrane fractions was quantified by Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich) and equilibrated between different samples ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein was measured using the protein extract for PEPCK activity using Bradford reagent (Millipore Sigma; Cat: B6916) and commercial bovine serum albumin standards (Thermo Fisher 23208) ...
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bioRxiv - Developmental Biology 2021Quote: ... Total proteins were extracted from cells with the M-PER Mammalian Protein Extraction Reagent (Sigma, GE28-9412-79) supplemented with protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... Fc chimeric proteins were purified via affinity based Fast Protein Liquid chromatography using Prosep® Ultra resin (Millipore). Fc conjugated proteins were eluted with 0.1 M glycine (pH 2.5 ...
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bioRxiv - Microbiology 2019Quote: ... PTP-tagged proteins were detected by incubating with anti-protein A serum (1:3000, 1 h) (Sigma, P3775) followed by Alexa Fluor® 594 goat anti-rabbit (1:250 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was measured using Pierce™ BCA Protein Assay Kit (Sigma-Aldrich, St. Louis, Missouri United States) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... solium larval extracts were assessed for protein concentration using a BCA or Bradford protein assay kit (Sigma-Aldrich), respectively.
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bioRxiv - Systems Biology 2021Quote: ... virus protein-bound host proteins were enriched by adding 50 µl anti-HA-agarose slurry (Sigma-Aldrich, A2095) with constant agitation for 3 hours at 4°C ...