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Citations for Millipore Sigma :
1401 - 1450 of 2589 citations for Apolipoprotein C2 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... diluted in 0.1M Tris-buffered saline and 0.5% (w/v) Roche Blocking Reagent (catalog #11096176001, Sigma-Aldrich). On the following day ...
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bioRxiv - Microbiology 2023Quote: ... After blocking and washing the slides with PBS with 0.05% TWEEN® 20 (Merck-Millipore, Burlington, USA), plasma from bloodclots or serum was diluted 1:32 (v/v ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... diluted to 1:1000 in blocking solution or rabbit anti-tyrosine hydroxylase (TH; Millipore #AB152, Burlington, MA) diluted to 1:1000 in blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... the neurons were permeabilized in blocking solution [0.5% w/v Triton X-100 (Sigma-Aldrich, X100-1L), 10% v/v normal donkey serum (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... Thorough washing was followed by blocking for 1 hour in 1% human serum albumin (HSA; Sigma-Aldrich) and 0.03-0.1% Triton X-100 dissolved in TBS ...
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bioRxiv - Neuroscience 2023Quote: ... Blocking was performed with 5% (vol/vol) NGS and 0.1% (vol/vol) Triton X-100 (Sigma-Aldrich) in phosphate buffered saline (PBS ...
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bioRxiv - Genetics 2023Quote: ... Blocking of the membrane was performed using 4% milk in TBS 0,5% Tween 20 (Sigma Aldrich, P7949) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies diluted in blocking solution were used: rabbit-anti-Sox9 (Millipore #AB5535, 1:300), rabbit-anti-Eomes (Abcam #ab23345 ...
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bioRxiv - Bioengineering 2023Quote: ... the blocking procedure was performed using 300μl per well of 2% HSA (Sigma, St. Louis, MO, USA) in PBS for 2hs ...
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bioRxiv - Bioengineering 2023Quote: ... the blocking procedure was performed using 300μl per well of 2% HSA (Sigma, St. Louis, MO, USA) in PBS for 2hs ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was then blocked for at least 1 hour in 1x casein blocking buffer (Sigma–Aldrich). To detect His-tagged proteins ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in PBST and a blocking solution added (donkey serum, Sigma-Aldrich, St. Louis, MO). Slices were then incubated overnight with the primary antibodies (1:500 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... followed by incubation in blocking solution (5% NDS; Jackson Immuno Research/0,3% Triton X-100; Sigma Aldrich) in PBS for 2h at room temperature (RT) ...
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bioRxiv - Molecular Biology 2023Quote: ... After blocking the membrane with 1% BSA in TBS and 0.1% Tween (Sigma-Aldrich, St. Louis, Missouri) (TBS-T ...
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bioRxiv - Immunology 2023Quote: ... followed by primary antibody prepared in StartingBlock buffer or Odyssey Blocking Buffer with 0.1% Tween-20 (Sigma). Depending on the experiment ...
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bioRxiv - Physiology 2023Quote: ... followed by overnight incubation at 4°C in blocking buffer containing mouse anti-vimentin antibody (Sigma, MAB3400) diluted 1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... free-floating tissue sections were incubated in blocking buffer (0.5% Triton X-100 (Sigma-Aldrich, T8787-100ML) + 10% normal horse serum (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The sections were blocked by covering the tissue in blocking buffer (1:10 goat serum (Sigma, USA) in Tris-buffered Saline (TBS ...
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bioRxiv - Genetics 2023Quote: ... Cells are then blocked with 2 mL blocking solution made of 5% BSA (cat#A3059-10G Sigma) and 0.05% Triton X-100 in PBS for 2 hours at RT or 4°C on a shaker.
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bioRxiv - Cell Biology 2023Quote: ... followed by 30 minute-long blocking-permeabilization treatment in 0.1% Triton X-100 (Sigma-Aldrich, Cat# T8787) solution with 10% BSA (Fisher ...
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bioRxiv - Bioengineering 2023Quote: ... and rinsed with 1×PBS before blocking and permeabilizing with 2% bovine serum albumin (BSA, Sigma-Aldrich) in 0.2% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... CC10 and involucrin staining protocols included an additional 30-minute blocking step with casein (Sigma-Aldrich, B6429) and 5% bovine serum ...
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bioRxiv - Neuroscience 2023Quote: ... Cells or tissues were lysed in blocking buffer containing 10 mM MMTS (S-methyl methanethiosulfonate, Sigma-Aldrich), 2.5% SDS ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were blocked with blocking solution (0.2% Fish skin gelatin [Sigma, G7765] and 0.5% BSA, sterile filtered) for 30 minutes in a humidified chamber ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was discarded and pelleted nuclei were resuspended in 3 ml blocking buffer (1xPBS [Sigma-Aldrich, P4417-50TAB ...
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bioRxiv - Neuroscience 2024Quote: ... followed by incubation in blocking solution (5% NDS; Jackson Immuno Research/0,3% Triton X-100; Sigma Aldrich) in PBS for 2 h at room temperature (RT) ...
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bioRxiv - Bioengineering 2024Quote: ... followed by blocking for 1 hour at room temperature in PBST plus 5% donkey serum (Sigma D9663). Slides were then incubated either 2 hours at room temperature or overnight at 4°C with the primary antibody diluted in DPBS containing 1% BSA and 0.5% Triton-X 100 ...
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bioRxiv - Molecular Biology 2021Quote: ... except that elution of bound proteins was done using 100 μg/ml FLAG peptide (Sigma, F3290).44 In brief ...
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bioRxiv - Neuroscience 2021Quote: ... The Aβ stock solution was prepared by solubilizing the Aβ25-35 peptide (Sigma-Aldrich; Merck KGaA) in sterile deionized water to a concentration of 1 mM and then incubated at 37°C for 3 days to allow self-aggregation before treatment ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were diluted 1 uL into 3 uL water before monitoring peptide formation electrophoretic TLC (Millipore). TLC plates were equilibrated with pyridine acetate buffer (5 mL pyridine ...
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bioRxiv - Cancer Biology 2019Quote: ... with the exception that the elution was performed using X3 Flag peptide (#F4799-4MG, Sigma, USA). Then 5x Non-Reducing Lane Marker Sample Buffer (#1859594 ...
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bioRxiv - Neuroscience 2020Quote: ... The Aβ stock solution was prepared by solubilizing the Aβ25-35 peptide (Sigma-Aldrich; Merck KGaA) in sterile deionized water to a concentration of 1 mM and then incubated at 37°C for 3 days to allow self-aggregation before treatment ...
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bioRxiv - Biochemistry 2021Quote: ... Immunoprecipitated proteins were eluted overnight at 4 °C with 50 μg of Flag peptide (Sigma-Aldrich) in 100 μL complemented non-denaturing lysis buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... The trypsinized peptides were vacuum-desiccated and solubilized in 0.1% trifluoroacetic acid (TFA; 299537, Sigma-Aldrich). The sample was then desalted using C18 Ziptip (ZTC18S960 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein was eluted twice from resin using 100μL of 500ng/μL [3x flag peptide (Sigma F4799), Myc (Pierce 20170) ...
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bioRxiv - Cell Biology 2021Quote: ... and bound proteins were eluted with 25 µl 1 mg/ml 3 × FLAG peptide (Sigma, F4799) in IP buffer containing 1% NP-40 and 1 × protease inhibitors at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... The digested peptides were further filtered with Amicon ultra centrifugal filters (10kD MWKO, Merck Millipore, MA), dried under vacuum extensively to remove residual primary amine from reagents ...
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bioRxiv - Plant Biology 2020Quote: ... Bound effectors and associated plant proteins were competitively eluted by 3XFLAG peptides (Sigma-Aldrich, Dorsett, England) and separated by SDS-PAGE ...
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bioRxiv - Microbiology 2021Quote: ... The retained complexes were eluted twice with either 3x FLAG peptide (200 μg/ml Sigma-Aldrich), HA peptide (400 μg/ml Roche ...
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bioRxiv - Systems Biology 2021Quote: ... 2 pmol (2 µL of stock) of the non-labelled AQUA® peptide HLEAAKGYSFTTTAEKAAELHK (Sigma-Aldrich) containing the quantification tag sequence GYSFTTTAEK was added to enable quantification of SIL-protein stock concentrations using MS analysis based on the ratio of heavy-to-light GYSFTTTAEK signals (see below) ...
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bioRxiv - Molecular Biology 2020Quote: ... One milligram of peptide was dissolved in 20 ul of 10% trifluoroacetic acid (TFA, Sigma-Aldrich), in an H218O base (95-98% 18O ...
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bioRxiv - Microbiology 2020Quote: ... Dried peptides were resuspended in a loading buffer consisting of 20 mM citric acid (Sigma-Aldrich) and 1% formic acid (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... Alkylated peptides were acidified with 1 μL of trifluoroacetic acid (TFA) (Fluka BioChemika, Sigma-Aldrich, GmbH) to inhibit trypsin and acidify peptides for desalting using C-18 StageTips ...
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bioRxiv - Biophysics 2020Quote: ... N-terminally labelled PR25 was obtained by reacting the peptide with amine-reactive AlexaFluor546 (Sigma-Aldrich). PolyU RNA with a molecular weight range from 800– 1,000 kDa was purchased from Sigma-Aldrich ...
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bioRxiv - Systems Biology 2022Quote: ... Peptide fractions were eluted using MeOH buffer containing trifluoroacetic acid (TFA) (Merck Sigma, Cat. No. 302031), FA or ABC ...
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bioRxiv - Biochemistry 2022Quote: ... The resin was incubated with 1 mL of 0.5 mg/mL 3x FLAG peptide (Sigma-Aldrich) in B-0.1 and rotated at 4°C for 1 h to elute the tagged remodeler ...
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bioRxiv - Immunology 2022Quote: ... The proteolytically cleaved peptides were extracted and desalted using ZipTip μC18 tips (Merck Millipore, Darmstadt, Germany). The peptide lysates were re-suspended in 15 µl 0.1% formic acid and analysed by nanoliquid chromatography mass spectrometry (UltiMate 3000 RSLCnano ...
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bioRxiv - Neuroscience 2021Quote: ... Peptides extracted from trypsin digestion were purified with ZipTip C18 pipette tips (Millipore, Billerica, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The protein complexes were specifically eluted with 125 mkl of 100 ng/mkl 3xFLAG peptide (Sigma) solution in TBS during 30 min on-ice incubation with agitation ...
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bioRxiv - Synthetic Biology 2022Quote: ... the synthesized peptide microchip was incubated with 100 ug/ml guinea pig liver tissue transglutaminase (Sigma) and 3 mM dansyl-cadaverine (Sigma ...