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Citations for Millipore Sigma :
1301 - 1350 of 2589 citations for Apolipoprotein C2 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... Purification and concentration of peptides was performed using C18 Zip Tip Solid Phase Extraction (Millipore). The peptides recovered were reconstituted into a final concentration of 0.5μg/μL of 2% ACN ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged proteins were eluted by 60 μl of 3x FLAG® Peptide (Sigma F4799) (diluted in Tris-buffered saline (TBS ...
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bioRxiv - Neuroscience 2021Quote: ... Peptides were dissolved in 80% ACN/5%TFA with 1 M glycolic acid (Sigma-Aldrich) and incubated with 0.6 mg titanium dioxide (TiO2 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal antibodies to anti-calcitonin gene-related peptide (CGRP; 1:500; C7113, Sigma Aldrich), rat monoclonal antibodies to substance P (SP ...
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bioRxiv - Microbiology 2021Quote: ... 100 μl of elution buffer (150 μg/ml 3X-FLAG peptide, Sigma, in Buffer 2) was added and samples were subjected to end-over-end rotation at 4 °C for 30 min ...
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bioRxiv - Biophysics 2020Quote: ... Protein was eluted with Myosin Buffer supplemented with 100 μg/mL Flag peptide (Sigma-Aldrich). The eluent was buffer-exchanged to Storage Buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were eluted with 300 μl of 250 μg/ml 3xFLAG peptide (Sigma, cat#F4799) in IP buffer without NP40 for 30 min at 25°C ...
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bioRxiv - Molecular Biology 2022Quote: ... and the ATAD5-RLC proteins were eluted with 100 µg/mL FLAG-peptide (Sigma Aldrich) in the same buffer ...
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bioRxiv - Bioengineering 2022Quote: ... The concentration of free thiols in all peptides was determined using Ellman’s reagent (Sigma Aldrich).
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bioRxiv - Plant Biology 2020Quote: ... bound complexes were eluted with 100-120 µl of FLAG3x peptide (100 µg / mL)(Sigma) in 0.2% digitonin containing TGS buffer for 40 min at 4°C.
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bioRxiv - Molecular Biology 2019Quote: ... Beads were then eluted twice10 min in 250μl elution buffer (150ng/μl 3xFLAG peptide (Sigma)) in low salt wash buffer supplemented with RNasin) ...
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bioRxiv - Plant Biology 2019Quote: ... Peptides were resuspended in 0.1% formic acid and desalted using C18 ZipTips (Millipore, Burlington, MA), and analyzed by LC-MS/MS.
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bioRxiv - Biochemistry 2020Quote: Recombinant INO80 complex was eluted with buffer B containing 1.6 mg Flag Peptide (Sigma-Aldrich). Anion exchange chromatography (MonoQ 5/50 GL ...
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bioRxiv - Cell Biology 2021Quote: ... Precipitated complexes were washed with lysis buffer and eluted with the FLAG peptide (Sigma, F4799) at a concentration of 400 μg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting peptide mixtures were loaded on a solid phase C18 ZipTip (Millipore, Billerica, MA) and washed with 35 μL 0.005% heptafluorobutyric acid/5% FA (vol/vol ...
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bioRxiv - Neuroscience 2021Quote: ... full length human NPY peptide (10 pmol in 1x PBS, Sigma-Aldrich, Cat. No. N5017), anti-CART antibody (1:500 ...
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bioRxiv - Biophysics 2021Quote: Proteins and peptides were labelled at their N-terminus with Fluorescein 5-Isothiocyanate (FITC, Sigma), purified and quantified following a described protocol [45] ...
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bioRxiv - Neuroscience 2021Quote: ... Peptides from both consecutive digestions were recovered by solid phase extraction using C18 ZipTips (Millipore), eluted in 2x7 μl aliquots of 50% MeCN 0.1% formic acid ...
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bioRxiv - Cell Biology 2020Quote: ... The bound protein was eluted four times with 250 μL of HA peptide (Sigma-Aldrich) (0,1 mg/ml).
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bioRxiv - Cell Biology 2020Quote: ... Peptides from both consecutive digestions were recovered by solid phase extraction using C18 ZipTips (Millipore), and resuspended in 0.1% formic acid for analysis by LC-MS/MS ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). After cell lysis ...
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bioRxiv - Developmental Biology 2020Quote: ... and then eluted with 200μl of wash buffer containing 0.4mg/ml FLAG peptide (Millipore Sigma). Eluted proteins were mixed with 2xSDS sample buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Puromycin-labeled peptides were identified using the mouse monoclonal antibody 12D10 (EMD Millipore Catalog# MABE343). Relative protein synthesis levels were determined by densitometry analysis of whole lanes.
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bioRxiv - Cell Biology 2021Quote: ... Peptides were eluted from the C18 SPE in 30 µL 50% acetonitrile (ACN, Sigma, 03150391) and 0.1% formic acid (FA ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mg of the lyophilized Aβ42 peptide was resuspended in 300 μl of 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP, Sigma-Aldrich). The mixture was sonicated and vortexed thoroughly to ensure proper solvation ...
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bioRxiv - Molecular Biology 2020Quote: ... The bound proteins were neutrally eluted with 50 µL of V5 peptide (V7754-4MG, SIGMA) at 1mg/mL in PBS ...
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bioRxiv - Immunology 2022Quote: ... Bound proteins were eluted by incubating the beads with 200µg/ml 3xFLAG peptide (Sigma- Aldrich) in IPP150 containing 0.02% NP-40 ...
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bioRxiv - Plant Biology 2022Quote: ... Elution of immunoprecipitated proteins was performed using 100 μg/mL c-Myc peptide (Sigma, M2435), on ice for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 0.05% Triton X-100) three times and eluted with 3XFLAG peptide (Sigma-Aldrich, cat# F3290). Elution fraction was then subjected to HA beads ...
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bioRxiv - Neuroscience 2022Quote: ... each peptide film was reconstituted in dimethyl sulfoxide (DMSO, Hybri-Max Cat# D2650, Millipore Sigma) to obtain a 1 mM Aβ stock solution ...
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bioRxiv - Microbiology 2023Quote: ... Co-immunoprecipitated proteins were eluted for 1h at 4°C with 3X FLAG peptide (Sigma) in TBS (pH 7.4) ...
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bioRxiv - Neuroscience 2022Quote: ... Purification and concentration of peptides was performed using C18 Zip Tip Solid Phase Extraction (Millipore). The peptides recovered were reconstituted into a final concentration of 0.5µg/µL of 2% acetonitrile ...
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bioRxiv - Neuroscience 2022Quote: ... Purification and concentration of peptides was performed using C18 Zip Tip Solid Phase Extraction (Millipore). Peptides recovered from in-gel digestion processing were reconstituted into a final concentration of 0.5µg/µL of 2% acetonitrile ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-proteins were eluted with 50 µL 500 ng/mL 3xFLAG peptide (Sigma, Cat. # F4799) at RT for 1h and supplemented with 5x SDS sample buffer (250 mM Tris-HCl pH 6.8 ...
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bioRxiv - Cell Biology 2024Quote: ... Anti-FLAG M2 Agarose Affinity Gel (A2220) and 3xFLAG Peptide (F4799) were from Sigma-Aldrich. GFP-Nanoab-Agarose was from Lablead (GNA-50-1000 ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were acidified with formic acid (pH < 3) and then desalted using C18 ZipTips (Millipore) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were eluted by incubation with 25 µL of 100 µg/ml 3xFLAG peptide (Sigma) in 0.05% RapiGest (Waters Corp ...
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bioRxiv - Biophysics 2023Quote: ... Protein was eluted with wash buffer supplemented with 100 mg/mL Flag peptide (Sigma-Aldrich). The eluent was buffer-exchanged to a storage buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... The peptides were desalted and fractionated using 200 µl Pierce C18 column tips (Merck Millipore).
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bioRxiv - Evolutionary Biology 2023Quote: ... the digested peptides of both mucus and bodies were desalted using C18 ZipTips columns (Millipore) and concentrated using a CentriVap (Labconco Corporation).
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bioRxiv - Microbiology 2023Quote: ... and eluted with FLAG peptide in PI-RIPA according to the manufacturer’s instructions (Milipore-Sigma). Cell lysates were separated with 10% SDS-PAGE gels ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by elution using lysis buffer containing 0.5 mg/ml 3X FLAG Peptide (Sigma Aldrich) (FLAG-p97) ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.4 containing 3x Flag peptide in final concentration 150 μg ml-1 (Sigma-Aldrich) for 1.5 hours at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction was either performed with the indicated concentrations of peptides or DMSO (Sigma Aldrich) as the vehicle control ...
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bioRxiv - Immunology 2024Quote: ... polystyrene flow tubes (Falcon #352054) were coated with PEPTIDE-2000 (pRGD, Sigma Aldrich, Lot SLCJ0890) for 1 hr at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... 50 mL of blocking buffer was prepared by adding 1 g of biotin-free BSA (Sigma Aldrich) and 200 μL of 0.5 M pH 8.0 EDTA (ThermoFisher ...
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bioRxiv - Cell Biology 2019Quote: ... and transferred to nitrocellulose membrane before blocking and with 5% milk (Marvel) or bovine serum albumin (Sigma) w/v in PBS-T (0.1% Tween-20 in PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... the flow lane was flushed with 100 uM oligonucleotide MS_204 in blocking buffer + 0.2 mM chloroquine (Sigma) at 100 ul/min.
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4 C in blocking solution containing rabbit anti-NeuN (Millipore, ABN78) primary antibody at 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed with 0,05% PBS-Tween 20 and blocked with Duolink® Blocking solution (Sigma-Aldrich) for 1 h at 37ºC ...