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Citations for Millipore Sigma :
1351 - 1400 of 10000+ citations for Tumor necrosis factor receptor superfamily member 3 TNFRSF3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Expression of pAgos was verified by Western blot with antibodies against His-tag (Sigma) after cell growth for 2 hours at 30 °C ...
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bioRxiv - Bioengineering 2022Quote: ... and mVenus-SpyCatcher-6×His were prepared using Gibson assembly with pET-19b (Novagen) as the plasmid vector ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Mpp75Aa1.1 or Mpp75Aa1.1-His was mixed with high purity pepsin (Sigma, St. Louis, MO) in 2 mg/ml NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... and dilutions were as follows (in parenthesis): His (mouse, Sigma cat# H1029; 1:2,500); β-actin (rabbit ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS-hi; Sigma, Cat. no. F7524), 1% antibiotic-antimycotic (AA ...
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bioRxiv - Biophysics 2024Quote: ... Protein was initially purified using affinity chromatography using HIS-Select Cobalt Affinity Gel (Sigma) on a gravity flow column ...
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bioRxiv - Microbiology 2024Quote: ... Purified His-tagged proteins were concentrated and desalted using Amicon centrifugal filters (Millipore, UFC500396) in TBS buffer and stored in storage buffer (30 mM Tris–HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Cancer Biology 2019Quote: Cell-derived from zebrafish brains and tumor brains were seeded on poly-D Lysine (1µg/mL) (Sigma-Aldrich) slides 1 hour before starting the experiment ...
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bioRxiv - Cancer Biology 2020Quote: RNA was extracted from cell lines and homogenized tumors using a GenElute Mammalian Total RNA Purification Kit (Sigma) according to standard protocols ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse pituitary corticotrope tumor cells AtT20 (from Dr. Hans-Peter Hauri, Biozentrum) were grown in Dulbeccco’s modified Eagle’s medium (DMEM, Sigma) containing 4500 mg/l (Neuro-2a ...
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bioRxiv - Cancer Biology 2020Quote: ... tumors were harvested and enzymatically disassociated using the Animal free Collagenase/Dispase Blend II reagent (Millipore, Burlington, MA). Cells were maintained in culture with RPMI-1640 media (Gibco ...
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bioRxiv - Bioengineering 2020Quote: ... Tumor tissue was dissected into fragments of 1-2mm3 and frozen in 90% fetal calf serum (FCS, Sigma) and 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Cancer Biology 2020Quote: ... and zebrafish tumors were mechanically homogenized in RIPA lysis buffer containing 1:100 protease inhibitors (P8340, Sigma-Aldrich) and 20 μM N-ethylmaleimide (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... P511 tumors were cut into pieces and digested with 1mg/ml collagenase I and 100μg/ml DNAse (Sigma) for 30 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Mechanically disaggregated tumors were enzymatically digested using a solution of 1mg/ml of collagenase IV (Sigma-Aldrich #C5138) and 200µg/ml of DNase I (Sigma-Aldrich #11284932001 ...
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bioRxiv - Molecular Biology 2023Quote: ... is described in (34) and was maintained as tumor spheres in stem cell-permissive Dulbecco’s modified Eagle’s medium/F12 medium (Sigma) supplemented with human recombinant epidermal growth factor (EGF ...
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bioRxiv - Cancer Biology 2023Quote: ... For RNA experiments 4 tumors of each experimental group were dissociated with collagenase IV (50 mg/mL) (Sigma) and a tissue chopper (Ted Pella ...
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bioRxiv - Immunology 2023Quote: ... Tumors were chopped and digested in 5 mL of PBS with 435 U/mL DNase (Sigma-Aldrich, D5025) and 218 U/mL collagenase (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... tumor tissue was cut into pieces and enzymatically digested with 80 µg/ml of collagenase D (Sigma-Aldrich) and 25 µg/ml of DNAse I (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: Tumors were resected from euthanized mice and immediately fixed in neutral buffered formalin (HT5012, Sigma-Aldrich, Burlington, MA) for 48h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... DAB (3-3’-diaminobenzidine, Sigma-Aldrich catalog no. D8001-10G) was dissolved in 0.1 N HCl at a concentration of 5.4 mg ml-1 and subsequently diluted ten-fold into sodium cacodylate buffer (pH 7.4 ...
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bioRxiv - Developmental Biology 2022Quote: ... The reaction was developed using 3-3’-Diaminobenzidine (DAB) (Sigma). For CLASP1 immunostaining ...
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bioRxiv - Cancer Biology 2019Quote: ... 3-hydroxybutyrate (3-OHB) was also purchased from Sigma (#166898).
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bioRxiv - Biochemistry 2021Quote: ... 2-amino-3-phosphonopropionic acid (AP-3, Sigma-Aldrich A4910), ATP (Sigma-Aldrich A2383) ...
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bioRxiv - Cell Biology 2019Quote: ... injected with 3 mL of 3% thioglycollate (Sigma-Aldrich, T9032). After 3 days ...
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bioRxiv - Immunology 2020Quote: ... E-64 and 3-Methyladenine (3-MA) were from Sigma. Necrostatin-1 was from Abcam ...
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bioRxiv - Immunology 2024Quote: ... 3-nitropropionic acid (3-NP, several doses, Sigma Aldrich N5636), Atpenin-A5 (several doses ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 10 mg/mL 3-iodo-L-tyrosine (3-IY, Sigma), and 0.5 mM all-trans-retinal for 2–3 days at 25°C in the dark prior to behavioral testing ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC; Sigma Aldrich #39391), and 3NPH solutions were mixed ...
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bioRxiv - Neuroscience 2023Quote: ... (3) the PDE4B inhibitor A33 (3 mg/kg, Sigma-Aldrich), (4 ...
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bioRxiv - Molecular Biology 2024Quote: ... Glycogen Synthase Kinase 3 (GSK3) inhibitor CHIR99021 (3 μM) (Millipore) and Leukaemia-Inhibitory Factor (LIF ...
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bioRxiv - Immunology 2021Quote: HEK293 and transfected derivatives were left unstimulated or stimulated for 24h with 10 ng/mL lipopolysaccharide (LPS) from Salmonella (Sigma), 10 ug/mL isolated S protein ...
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bioRxiv - Neuroscience 2020Quote: ... the HEK293 and primary neuronal cells were plated on the 12mm #1 coverslips coated with poly-D-lysine (Sigma-Aldrich). For the 96-well plate screening assays ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells were seeded at a low density on 0.01% (w/v) poly-L-lysine (Sigma, St Louis, MO, USA) coated coverslips and were transfected the subsequent day using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... The exogenous expression of ACE2 and TMPRSS2 in the HEK293-C34 cells was induced by addition of doxycycline hydrochloride (1 μg/ml) (Sigma). TMPRSS2-expressing Vero E6 (VeroE6/TMPRSS2 ...
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bioRxiv - Molecular Biology 2020Quote: pCDNA3 vectors expressing HA-tagged full size LARP6 or La-domain were transfected into HEK293 cells and 24h after transfection the cells were fixed and incubated with anti-HA primary antibody (Sigma), followed by incubation with fluorescent secondary antibody ...
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bioRxiv - Immunology 2019Quote: ... Vectors were transiently transfected in HEK293-6E cells and antibodies were purified from supernatant using Robocolumn Eshmuno A (EMD Millipore) or Monofinity A Resin prepacked columns (Genscript) ...
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bioRxiv - Microbiology 2020Quote: HEK293 cells were transfected with plasmids encoding different ACE2 orthologs (Table S1) by polyetherimide (PEI) (Sigma, St Louis, MO, USA). After 40 hrs incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... thereafter named HEK293) were cultured under 5% CO2 at 37°C in low-glucose Dulbecco’s modified Eagle’s medium (DMEM) (Sigma–Aldrich, D6046) supplemented with 10% foetal bovine serum (FBS-Gibco) ...
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bioRxiv - Cell Biology 2024Quote: Doxycycline-inducible stable Flp-In T-REx HEK293 cell lines expressing mitochondrial-relocated golgin-BirA* fusion proteins were induced with 1 μg/ml doxycycline (Sigma) in culture medium once they reached approximately 80% confluency ...
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bioRxiv - Cell Biology 2023Quote: 2×10^5 Vero E6 cells or HEK293-ACE-2 were cultured in 24-well plates in Dulbecco’s Modified Eagle medium (DMEM, Sigma D5796) containing 10% of fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: ... and GIGYF2-KO TLR3-HEK293 cells were either mock-treated or transfected with 1 μg/mL Poly(I:C) (Sigma, P1530) using Lipofectamine2000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Then pSIREN-RetroQ-Sirt7 and the negative control vector (pSIREN-RetroQ) were transfected into HEK293 cells using PEI transfection reagent (Sigma). The pSIREN-RetroQ-Sirt7 and the negative control vector (pSIREN-RetroQ ...
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bioRxiv - Biochemistry 2023Quote: HEK293 Flp-In T-Rex cells were cultured in DMEM high glucose medium supplemented with 10% FBS (Sigma-Aldrich F0804), 2mM L-glutamine (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: PFKFB3 depletion was carried out in HEK293 cells using siRNAs (80 nM; transfected twice on consecutive days) from SIGMA (SASI_Hs01_00065120). The cells were treated with control or specific siRNA and lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... containing DMEM/F12 supplemented with 20 ng/ml epidermal growth factor (EGF, Sigma-Aldrich), 20 ng/ml basic fibroblast growth factor (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2021Quote: ... Growth factors were solubilized in 0.2% Bovine Serum Albumin (BSA) (Sigma Millipore #A2934-100G) and stored at -20°C in single-use aliquots prior to use in epithelial cell cultures ...
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bioRxiv - Cell Biology 2020Quote: ... the medium was supplemented with mLIF (106 U/mL murine leukemia inhibitory factor; Millipore). mESC were split every 2 to 3 days ...
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bioRxiv - Immunology 2020Quote: ... and 1ng/ml basic fibroblast growth factor (Sigma-Aldrich, St. Louis, MO, USA, F0291). For the first 48h ...