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Citations for Millipore Sigma :
1351 - 1400 of 10000+ citations for Rat TGF beta 1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... rats received daily subcutaneous injections of 10 μg estrone (Sigma-Aldrich) in 0.1 ml sesame oil ...
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bioRxiv - Neuroscience 2023Quote: ... Rats were slowly infused with urethane (0.84 g/kg; 102452447, Sigma) (diluted in saline at 150 mg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... rat monoclonal anti-HA-Peroxidase high affinity (Roche, Sigma-Aldrich, 12013819001), rabbit polyclonal anti-Actin (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Control mice received 250µg of IgG from rat serum (Sigma, 18015) i.p ...
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bioRxiv - Neuroscience 2023Quote: ... . Rats were anaesthetized with either ketamine (100 mg/kg, i.p., Sigma) and xylazine (10 mg/kg ...
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bioRxiv - Neuroscience 2023Quote: ... a rat monoclonal anti-poly(GR) antibody (clone 5A2, MABN778, Millipore), a Living Colors® EGFP mouse monoclonal antibody (632569 ...
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bioRxiv - Biochemistry 2023Quote: ... HA clone 3F10 (rat monoclonal, conjugated to FITC, Sigma-Aldrich / Merck), GFP (mouse monoclonal ...
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bioRxiv - Microbiology 2023Quote: ... Each membrane was incubated with rat anti-HA primary antibody (Sigma 11867423001 ...
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bioRxiv - Neuroscience 2023Quote: The following antibodies were used: anti-hemagglutinin (HA, rat, Sigma-Aldrich). For immunoblotting ...
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bioRxiv - Neuroscience 2024Quote: ... the Wistar rats were stereotactically injected with ibotenic acid (Sigma-Aldrich) into the striatum (Fig ...
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bioRxiv - Neuroscience 2021Quote: ... They were then incubated with secondary antibody (goat anti-mouse Alexa 488 or 555, goat anti-rabbit Alexa 488 or 555, and goat anti-rat Alexa 555, Sigma Aldrich, 1:500) and mounted with Antifade ...
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bioRxiv - Neuroscience 2023Quote: ... and an antibody against calcitonin gene-related peptide (CGRP; polyclonal rabbit anti-rat, 1:5000; #C8198, AB_259091; Sigma-Aldrich, St. Louis, MO, USA) or growth associated protein-43 (GAP43 ...
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bioRxiv - Genetics 2023Quote: ... Slides were incubated with goat anti-mouse collagen α1(XI) antibody at 1:500 dilution and mouse anti-rat PAX1(Cat #MABE1115M, Sigma-Aldrich, MO, USA), in PBS + 0.1% Triton + 1% normal goat serum at 4L°C overnight ...
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bioRxiv - Immunology 2024Quote: ... pH 7.6) + 0.05% v/v Triton-X100 and 10% donkey serum and subsequently incubated with rat-MLKL (Millipore MABC604 clone 3H1 1:1000) overnight ...
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bioRxiv - Neuroscience 2023Quote: Western blot analysis of MBP was performed on protein lysates from the anterior cerebrum at P22 as previously described [32] using rat anti-MBP (Millipore MAB386 1:500) and anti-β-actin (Sigma-Aldrich AC-74 ...
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bioRxiv - Biophysics 2021Quote: U2OS cell immunostained for microtubules for DNA-PAINT imaging were prepared as previously described6 using anti beta-tubulin antibody (T5293; Sigma-Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... Protein from gels were transferred onto PVDF membranes and probed with NSF (SYSY, mouse monoclonal) and beta actin (Sigma, mouse monoclonal) antibodies overnight with shaking ...
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bioRxiv - Physiology 2021Quote: Recipient mice were rendered diabetic before islet transplantation with a single intravenous injection of the beta cell toxin streptozotocin (STZ 200 mg/Kg; Sigma, MO) dissolved fresh in 100 mM sodium citrate (pH 4.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The beta subunit of complex V and proteins bound to it were immunoprecipitated using an antibody to the beta subunit of ATP synthase (Millipore, MAB3494) in accordance with the Pierce™ Co-Immunoprecipitation Kit (Cat.No:26149) ...
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bioRxiv - Biophysics 2020Quote: ... Primary antibody staining was carried out by placing coverslips upside down onto a drop of primary antibody staining mix (mouse-anti-beta-tubulin, catalog no. T5293, Sigma-Aldrich, diluted 1:300 in PBS containing 2% (w/v ...
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bioRxiv - Neuroscience 2022Quote: ... 810-2 was dissolved in a vehicle (VEH) solution of 20% hydroxy propyl beta cyclodextrin (HPβCD; Sigma, St. Louis, MO, USA) in acetate buffer [0.1 M acetic acid (from glacial acetic acid stock ...
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bioRxiv - Microbiology 2019Quote: ... Primers (Table S1) used for PCR amplification of the beta-lactams genes (blaTEM, blaSHV, blaCXT-M and blaOXA) were acquired from Sigma Aldrich, USA ...
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bioRxiv - Cell Biology 2020Quote: ... Intraperitoneal glucose tolerance test was performed by fasting overnight and injecting 2 g/kg D-glucose solution at 2 weeks after mouse beta cells were ablated with one high dose (150 mg/kg) of streptozotocin (Cat. No. S0130-1G, Sigma-Aldrich). Blood was collected in heparin-coated tubes at fed state ...
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bioRxiv - Neuroscience 2021Quote: ... The biochemical assay of GCase activity was carried out in a buffer containing 4-methylumbelliferyl beta-d-glucopyranoside substrate (Cat. M3633, Sigma Aldrich); after 1 hour at 37°C the reaction was stopped with 0.25 M glycine buffer pH 10.4 and the fluorescence emission of the 4-methylumbelliferyl generated (4-MU ...
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bioRxiv - Bioengineering 2023Quote: ... for 5 minutes and suspended in medium (DMEM 85% v/v, Corning; FBS 15% v/v, VWR; 1x Penicillin-Streptomycin, VWR; 50 µM beta-mercaptoethanol, Sigma-Aldrich). 10,000 viable cells were plated per well in white polystyrene 384-well microplates (Greiner Bio-One ...
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bioRxiv - Plant Biology 2023Quote: ... except that 3 µl beta-mercaptoethanol per sample replaced 1,4-dithiothreitol (DTT) and 0.5% polyvinylpyrrolidone (PVP, mw 360 000, Sigma Aldrich, Steinheim, Germany) was added to the extraction buffer ...
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bioRxiv - Microbiology 2023Quote: ... about 50 μg proteins from each sample were reduced with 10 mM tris (2-carboxyethyl) phosphine (TCEP, Adamas-beta) and then alkylated with 40 mM iodoacetamide (IAA, Sigma-Aldrich). Proteins were pre-digested with 0.5 μg trypsin (Hualishi Tech ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were electrophoresed on polyacrylamide gels transferred onto PVDF membranes and probed using the following antibodies: mouse anti-beta-Actin (Sigma #A1978), mouse anti-Vinculin (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... using 1:4 phosphate buffer from the Total SOD kit (Sigma-Aldrich, kit 19160) for SOD activity ...
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bioRxiv - Cell Biology 2023Quote: Proteins were extracted from 50 hpf zebrafish embryos using the lysis buffer from the calpain 1/2 activity kit (InnoZyme™ Calpain 1/2 Activity Assay Kit CBA054 purchased from Merck Millipore). Protein concentration was adjusted to 2 mg/mL and calpain enzymatic activity was measured in microtubes following the manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Leptin and NPY were measured using commercially available ELISAs (all from Millipore, Temecula, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ELISA was performed using conditioned media (∼80% confluent cells, concentrated with Millipore UFC 900324 filters) and normalized by protein concentration (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2020Quote: ... The ELISA plates were washed and blocked with 5% Foetal Bovine Serum (FBS, Sigma, F9665) in 1x PBS/0.1% Tween 20 for 1 hour at room temperature ...
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bioRxiv - Physiology 2022Quote: ... Leptin content in media was analyzed by ELISA (Cat. # EZML-82K, Millipore Sigma, Danvers, MA).
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bioRxiv - Microbiology 2022Quote: ... Biotinylated proteins were captured in ELISA plates precoated with 5 μg/ml of avidin (Sigma) in Na2CO3-NaHCO3 buffer (pH 9.6 ...
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bioRxiv - Immunology 2024Quote: ... Nunc MaxiSorp ELISA plates were precoated with dsDNA (100 mg/mL, Sigma, St. Louis, MO) in phosphate-buffered saline (PBS ...
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bioRxiv - Physiology 2023Quote: ... and VEGF concentrations were measured by ELISA (R&D Systems, Mercodia, Abcam, and Sigma, respectively), and total plasma bile acids using a colorimetric assay (Abcam) ...
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bioRxiv - Synthetic Biology 2023Quote: ... were diluted in PBS and used to coat MaxiSorp 96-well ELISA plates (Sigma; M9410) for 1hr at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... 100 µl of a THP-1 cell suspension (106 cells/mL) in 20 mg/mL collagen from rat tail type I (Sigma Aldrich, St. Louis, USA) were spread in the basolateral surface of inverted inserts ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used in this study for immunoblotting were: rat anti-mouse MLKL (WEHI clone 5A6; produced in-house and soon available from Millipore as MABC1634; 1:2000)49 ...
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bioRxiv - Immunology 2022Quote: ... For surface staining cells were incubated with mastermix prepared in Fc-block (1%FCS/PBS with 0.1% rat IgG (Sigma-Aldrich, St. Louis, MO, USA)) ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were then incubated with slight agitation (40 rotations/min) overnight at 4°C with a rat anti-muscarinic acetylcholine receptor M2 (M2 AChR) primary antibody (1:500, Sigma-Aldrich, reference number MAB367) in the same blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... and a primary rat antibody against myelin basic protein (MBP) (1:50 in blocking solution; Cat. No. MAB386, Sigma-Aldrich, St. Louis, MO, USA). Following five 5-minute washes at 20°C with PBS ...
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bioRxiv - Immunology 2022Quote: ... penicillin-streptomycin (Hyclone, Chicago, IL), 10% heat-inactivated fetal bovine serum (Hyclone, Chicago, IL) and beta-mercaptoethanol (Sigma-Aldrich, St. Louis, MO). Cell lines have been recently authenticated and were periodically tested for mycoplasma species and were confirmed negative.
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bioRxiv - Neuroscience 2023Quote: ... Fixed cells were incubated with staining solution (1mg/mL of X-Gal,5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside; Sigma Aldrich #11680293001)(Lorenzo ...
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bioRxiv - Neuroscience 2023Quote: ... equivalent to about 5 mg/kg/day) was prepared in vehicle made of 0.45% (2-hydroxypropyl)-beta-cyclodextrin (β-CD; both from Sigma-Aldrich, France), and administered in drinking water for 28 days ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA/DNA and alpha/beta tubulin were tested using cellulose beads with immobilized single and double stranded calf thymus DNA (Sigma Aldrich, USA). 1 µg of PfCoSP was mixed with 500 µl cellulose matrix (1mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Microtubules were linked to PEG-silanized coverslips by incubating chambers with 33 μg/mL monoclonal anti-beta-tubulin III (neuronal) antibody (Sigma-Aldrich, T8578) for 5 minutes prior to washing with 10 mg/mL BSA for 2 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... on plates coated with Collagen Type I Rat Tail (Sigma-Aldrich, #C7661). Passages 4-6 were used for experiments ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies (chicken anti-TH, Abcam #76442; Rat anti DAT, Millipore MAB369) were diluted 1:1000 in washing buffer containing 0.3% Triton X-100 ...