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Citations for Millipore Sigma :
1351 - 1400 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 1 mL of lentiviral supernatant was added to 5×105 RAW 264.7 macrophages with 4 μg/mL of hexadimethrine bromide (Millipore) and cells were spinoculated at 300 x g for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... THP1 monocytes were differentiated to macrophages by treatment with 20 ng/ml phorbol myristate acetate (PMA) (Sigma-Aldrich P8139) for 20 hours followed by incubation in PMA free RPMI 1640 media for two days and used for infections ...
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bioRxiv - Immunology 2022Quote: The RAW 264.7 macrophage cell line was maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) high glucose (Sigma-Aldrich, D6546) with 10% fetal calf serum (FCS ...
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bioRxiv - Microbiology 2023Quote: ... in alveolar macrophage media (1X penicillin-streptomycin [Gibco, MA], 10% FBS [Gibco, MA]/0.1%β-mercaptoethanol [Sigma-Aldrich, MA]). After a 2 hour incubation at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... macrophages were washed and polarized toward M1 or M2 phenotypes by incubation with 40 ng/mL LPS (Sigma-Aldrich) and 100 ng/mL IFNγ (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... Primary peritoneal macrophages were obtained by peritoneal lavage of mice treated intraperitoneally with 4% thioglycolate (Sigma-Aldrich, Cat# T9032) for 4 days ...
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bioRxiv - Molecular Biology 2024Quote: ... Macrophages were characterized by cytospin and flow cytometry under basal conditions and following LPS (Sigma-Aldrich St. Louis, MO) stimulation (100 ng/nL for 72 hours ...
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P3HT-GRAPHENE DEVICE FOR THE RESTORATION OF VISUAL PROPERTIES IN A RAT MODEL OF RETINITIS PIGMENTOSAbioRxiv - Neuroscience 2022Quote: ... ionized calcium-binding adaptor molecule 1 (Iba-1, 1:1000; Wako Pure Chemical Industries, Japan) and glial fibrillary acidic protein (GFAP, 1:200; Sigma-Aldrich) in 1% NGS and 0.3% Triton-X-100 ...
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bioRxiv - Biophysics 2020Quote: Codon-optimized and his-tagged (C-terminus) genes for HG-series Kemp eliminases (Supplementary Table 1) cloned into the pET-11a vector (Novagen) via NdeI and BamHI were obtained from Genscript ...
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bioRxiv - Cell Biology 2020Quote: ... SOX2 and MYC genes with recommended MOI in 1 ml of erythroblast cell expansion media containing 10 μg/ml Polybrene (Sigma). After 24 hours of transduction the virus was removed ...
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bioRxiv - Molecular Biology 2019Quote: ... HEK293T were plated at 50% confluency on 60 mm dishes and transiently transfected with the gene specific shRNA pLKO.1 plasmid (Sigma), packaging plasmid (Δ8.9 ...
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bioRxiv - Biochemistry 2019Quote: ... with an N-terminal His6-tag followed by a TEV protease site and the full-length MRPP2 gene (SDR5C1) were cloned into a pCDFDuet-1 (Novagen) co-expression vector as previously described (41) ...
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bioRxiv - Molecular Biology 2021Quote: ... and J-Lat 11.1 (integrated full-length HIV-1 genome mutated in env gene and GFP replacing Nef) cells were cultured in RPMI-1640 medium (Sigma) supplemented with 10% FBS and 100 μg/ml penicillin-streptomycin at 37 °C in a humidified 95% air-5% CO2 atmosphere ...
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bioRxiv - Microbiology 2020Quote: ... The shRNA targeting the HPS-1 gene (TRCN0000292556) as well as the packaging plasmids pCMV-VSV-G and pCMV-dR8.91 were obtained from Sigma Aldrich. The plasmids encoding the shRNA sequences were pooled in equal concentrations and mixed with the packaging plasmids pCMV-VSV-G and pCMV-dR8.91 to generate lentiviral particles ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293T were plated at 50% confluency on 60 mm dishes and transiently transfected with the gene specific shRNA pLKO.1 plasmid (Sigma), packaging plasmid (Δ 8.9 ...
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bioRxiv - Neuroscience 2022Quote: Quantitative PCRs were run using qPCRBIO SyGreen mix separate-Rox (cat #: PB20.14-51 PCRBIOSYSTEMS) and specific primers (Table 1; Table 2) or Gapdh as housekeeping gene (Sigma-Aldrich), on a LightCycler 96 Real-Time PCR System (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... the tetracycline resistance gene from pACE2 (Geneva Biotech, Genève, CH) and the two MCSs from pACYCDuet™-1 (Novagen, Merck Millipore). Csx23 was inserted into MCS-1 of pRATDuet (NcoI/SalI ...
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bioRxiv - Cancer Biology 2023Quote: NKX3.1 expression was inhibited using siRNA against the NKX3.1 gene (WD10664038) as well as universal negative control SiRNA#1 (SIC001) was purchased from Sigma Aldrich. 70-90% of confluent cells were replaced with Opti-MEM ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293T were plated at 40% confluency on 60 mm dishes and transfected with the gene specific shRNA pLKO.1 plasmid (Sigma), packaging plasmid (Δ8.9 ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293T were plated at 50% confluency on 60 mm dishes and transiently transfected with the gene specific shRNA pLKO.1 plasmid (Sigma), packaging plasmid (Δ8.9 ...
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bioRxiv - Bioengineering 2022Quote: ... pSANG10-Avi clones were expressed in the same way in the presence of 50μM free D-biotin (Sigma).
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bioRxiv - Cell Biology 2021Quote: ... was expressed in COS-7 cells and immobilized to anti-T7 tag-antibody-conjugated agarose beads (EMD Millipore) (Matsui et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cleaved samples of the total expressed and soluble libraries were analyzed by SDS-PAGE and Western blotting (Sigma-Al-drich Monoclonal ANTI-FLAG® M2-Peroxidase (HRP ...
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bioRxiv - Biochemistry 2021Quote: Full length 6xHIS-Faf1 was expressed from pET28a-Faf1 in Rosetta (DE3) pLysS Competent Cells (Novagen, Merck Millipore) bacteria as above ...
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bioRxiv - Biochemistry 2021Quote: Full length 6xHIS-Faf1 was expressed from pET28a-Faf1 in Rosetta (DE3) pLysS Competent Cells (Novagen, Merck Millipore) bacteria as above ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-surfactant associated protein C (SFTPC, 1:1000, Millipore, AB3786), goat anti-podoplanin (PDPN ...
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bioRxiv - Neuroscience 2021Quote: ... and house-keeping protein GAPDH (1:500; Mouse; Sigma-Aldrich G8795) or β-actin (1:100 ...
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bioRxiv - Systems Biology 2019Quote: ... The 1 mg/mL pure bovine glutamate dehydrogenase (GDH) protein (Sigma) was reacted with 1 mM DSSO in 50 mM HEPES buffer ...
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bioRxiv - Plant Biology 2020Quote: ... Transferred proteins were probed with anti-tubulin (Sigma, 1:10,000 dilution) and anti-GFP (Takara Bio ...
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bioRxiv - Bioengineering 2021Quote: ... mouse anti-glial fibrillary acidic protein (GFAP)–Cy3 (Sigma, 1:2000) to show astrocyte structure ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was blotted with anti-Protein A (1:10,000, Sigma), anti-HA (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-Alzheimer Precursor Protein (APP) 22C11 (1:100, MAB348, Millipore), and mouse anti-β-Tub III/Tuj 1 (1:4000 ...
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bioRxiv - Cancer Biology 2020Quote: ... at 1 ug/ml or control protein Bovine Serum Albumin (Sigma) at 1 ug/ml in PBS were coated onto flat bottom ...
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bioRxiv - Plant Biology 2020Quote: ... The immunoprecipitated proteins were incubated with 1 μg MBP (Sigma-Aldrich) in kinase buffer (25 mM Tris-HCl ...
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bioRxiv - Physiology 2021Quote: ... breast cancer resistance protein (Bcrp-1:100; Merck Millipore, Massachusetts, USA) or Abcg1 (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... polyclonal goat anti-ribosomal protein L8 (SAB2500882, Sigma-Aldrich; 1:500), monoclonal mouse anti-β-Actin (GTX26276 ...
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bioRxiv - Developmental Biology 2022Quote: ... polyclonal rabbit anti-pro surfactant protein C (1:4000, Millipore, #AB3786), mouse monoclonal acetylated tubulin (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... overnight to degrade proteins and then with 1 mM PMSF (Sigma) to inactivate the Proteinase K ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit polyclonal Prosurfactant Protein C (1:200 pH9, AB3786, Merck Millipore), mouse monoclonal TTF-1 (clone SPT24 ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were detected with mouse anti-FLAG monoclonal (Sigma, 1/10000), rabbit anti-Rad51 polyclonal (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... stained with 1:200 mouse anti-ubiquitinylated proteins (Sigma #04-263) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-phosphoepitope SR proteins clone1H4 (Millipore, cat#MABE50, 1:2,000 dilution) were used as primary antibodies ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-pro-surfactant protein C (SFTPC, 1:1000, AB3786, Millipore), goat anti-SOX9 (SOX9 ...
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bioRxiv - Developmental Biology 2023Quote: ... protein lysates were prepared in 1× RIPA buffer (Millipore, #20-188) from Drosophila head tissue expressing UAS-FLAG-Dx ...
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bioRxiv - Genetics 2020Quote: ... Specific primers for mouse leptin and 2 mouse housekeeping genes used for normalization (β-actin and 34B4 mouse genes) were purchased from Sigma (Sigma, France). We used primers for Leptin (forward ...
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bioRxiv - Immunology 2019Quote: ... endogenous Asc from a total of 3.5 mg protein was immunoprecipitated with 1 μg of anti-Asc antibody and 60 μl of protein A/G agarose beads (Sigma), and bound Mul1 was detected with anti-Mul1 antibody by western blotting ...
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bioRxiv - Biophysics 2021Quote: ... The GB1 tag was cleaved from the oxidized proteins by incubating overnight with thrombin (10 U.mg−1 protein, Sigma-Aldrich). The cleaved protein was purified by reversed phase high-performance liquid chromatography (RP-HPLC ...
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bioRxiv - Biophysics 2020Quote: ... For reduction and alkylation of the proteins, proteins were incubated with SDC buffer (1% Sodiumdeoxycholate, 40nmM 2-Cloroacetamide (Sigma-Aldrich), 10 mM tris(2-carboxyethyl ...
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bioRxiv - Microbiology 2021Quote: ... The following antibodies were used for specific protein detection: anti-PAP (1: 1:5000, rabbit, Sigma), anti-myc (mouse ...
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bioRxiv - Biochemistry 2020Quote: ... and protein expression was induced with 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich) for 20-24h at 20°C ...