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Citations for Millipore Sigma :
1351 - 1400 of 2420 citations for RGS4 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... purified proteins were eluted twice with 50 ul of 1 mg/ml 3X flag peptide (Sigma) in co-IP buffer on the nutator for 10 minutes at room temperature.
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bioRxiv - Biochemistry 2022Quote: ... Two sequential elutions were carried out with 100 μl 0.1 mg/ml 3× Flag peptide (Sigma) in RNC buffer at room temperature for 25 min ...
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bioRxiv - Neuroscience 2023Quote: ... or Heparinase 1 + autocamtide-2-related inhibitory peptide (AIP, 0.17 μg/μl/site, Sigma-Aldrich, SCP0001) was injected into hippocampal CA1 area at a rate of 3 nl/s ...
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bioRxiv - Plant Biology 2022Quote: ... Captured proteins were eluted off FLAG beads using 500 μg/mL 3x-FLAG peptide (Sigma-Aldrich). Eluted proteins were then incubated with Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... bound proteins were eluted using buffer A containing 500ng/μL 3X FLAG® peptide (Sigma; F4799) for followed SDS-PAGE.
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bioRxiv - Microbiology 2023Quote: ... Bound protein was eluted in 30 μl of elution buffer (0.15 mg/ml 3xFLAG peptide [Sigma, St Louis ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG-tagged proteins were eluted from the beads with 200 µg/ml FLAG peptide (Sigma-Aldrich) in 0.5 ml of lysis buffer B ...
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bioRxiv - Systems Biology 2023Quote: ... peptide samples were loaded onto an Ascentis® ES-C18 Column (Sigma–Aldrich, St. Louis, MO) and separated with a 10 minute LC gradient (10% Buffer A (0.1% FA in water ...
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bioRxiv - Cell Biology 2022Quote: ... and immunoprecipitated proteins were eluted from Dynabeads by incubating with 200 μM FLAG peptides (Sigma-Aldrich) for 90 min ...
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bioRxiv - Immunology 2023Quote: ... The groups consisted of the 16 synthesized vaccine peptides with poly(I:C) (Sigma-Aldrich cat. #P1530), 16 synthesized vaccine peptides with STING agonist BI-1387446 ...
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bioRxiv - Biophysics 2023Quote: ... All other reagents applied in peptide synthesis were purchased from Sigma Aldrich(St. Louis, MO, USA). Sodium dihydrogen phosphate ...
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bioRxiv - Biophysics 2023Quote: ... peptides with the sequences SVEGEGEEEGEEY (α-CTT) and DATAEEEEDFGEEAEEEA (β-CTT) were purchased from Sigma-Aldrich and used without further purification ...
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bioRxiv - Microbiology 2023Quote: ... the immunoprecipitated fraction was eluted with 40 µL of 0.1 mg/mL 3xFlag peptide (Sigma-Aldrich) at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... 0.01/0.001% LMNG/CHS and eluted by adding 250 µg/ml 3xFLAG peptide (final concentration, Sigma). The total supernatant was analyzed via 10% SDS-PAGE ...
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bioRxiv - Bioengineering 2022Quote: ... calpain substrate peptide or conjugates were incubated with 26.6 nM human calpain-1 (Sigma-Aldrich, C6108) in 50 mM N-(2-hydroxyethyl)piperazine-N′-ethanesulfonic acid (HEPES) ...
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bioRxiv - Evolutionary Biology 2023Quote: (Bio-Techne: https://www.bio-techne.com/p/small-molecules-peptides/dtag-13_6605) was reconstituted in DMSO (Sigma) to a final concentration of 5 mM ...
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bioRxiv - Cell Biology 2022Quote: ... without any antibodies or the immunoprecipitation was outcompeted with the 3XFLAG peptide (340 μm; Sigma, F4799). Beads were then washed 6 times with Buffer A with 0.1% Triton X-100 followed by elution with Laemmli buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... predicted mature peptide regions of ZmRALF1/2/3/5 genes were cloned into plasmid pET32b (Novagen) with gene specific primers as indicated (Supplemental Table S1) ...
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bioRxiv - Microbiology 2023Quote: ... Purified peptides were characterized by MALDI TOF MS and analytical HPLC (Millipore Chromolith RP-18e column).
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bioRxiv - Genetics 2023Quote: ... Beads were washed five times with TEGN buffer and eluted with 3xFLAG peptide (F4799, Sigma-Aldrich) (45 µl ...
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bioRxiv - Synthetic Biology 2023Quote: ... peptide samples were loaded onto an Ascentis® ES-C18 Column (Sigma–Aldrich, St. Louis, MO) and separated with a 10 minute gradient from 98% solvent A (0.1 % FA in H2O ...
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bioRxiv - Molecular Biology 2023Quote: ... Flag-tagged proteins were eluted from the beads using 150 ng/ml of 3xFLAG peptide (Sigma) in 50–100 ul of elution buffer (20 mM HEPES-KOH (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: Aminopeptidase activity within live Dd2 parasites was determined using a fluorogenic H-Leu-NHMec peptide (Sigma) as described previously (52) ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were acidified and loaded onto p200 pipette tips packed with Empore SPE disks C18 (Sigma) and Oligo R3 Resin (Applied Biosystems) ...
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bioRxiv - Microbiology 2024Quote: ... after which the ylmH complexes were eluted using 0.2 mg ml−1 3× Flag peptide (Sigma) in wash buffer ...
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bioRxiv - Neuroscience 2024Quote: ... we pre-incubated it with the GnRH2 and GnRH3 peptides (SCP0183 and L4897 respectively, Sigma-Aldrich) at a concentration of 100 μg/mL ...
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bioRxiv - Microbiology 2024Quote: ... peptide samples were loaded onto an Ascentis® ES-C18 Column (Sigma–Aldrich, St. Louis, MO) and separated with a 10 min LC gradient (10% Buffer A (0.1% formic acid (FA ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were eluted in buffer 5 [buffer 3 plus 200 μM FLAG peptide (Sigma-Aldrich)] for 1.25 hours ...
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bioRxiv - Cell Biology 2024Quote: ... and ZmRALF12 coding sequences24 without signal peptides were amplified and cloned into the pET-32a (Novagen) vector ...
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bioRxiv - Cell Biology 2024Quote: ... and the peptide mixtures were desalted on C-18 reverse phase material (ZipTip μ-C18, Millipore). Eluted peptides were concentrated by evaporation under vacuum and subsequently resolved in 0.1% acetic acid / 2% acetonitrile containing HRM/iRT peptides (Biognosys ...
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bioRxiv - Neuroscience 2021Quote: Sections were first rinsed three times for 10 min in PBS and then incubated in blocking solution with 0.1M PBS containing 0.3% Triton X100 (Sigma) and 0.2% Bovine Serum Albumin (BSA ...
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bioRxiv - Developmental Biology 2021Quote: ... All samples were then incubated 30min at room temperature in blocking buffer (PBS + 1% BSA, A2153-10G, Sigma) followed with primary antibodies incubation in blocking buffer overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... blocking and permeabilization was performed with DPBS + 3% BSA + 0.1% Triton X-100 (Millipore Sigma cat. no. X100) for 30 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Sections where incubated overnight in blocking solution containing the primary antibodies (Goat anti-ChAT 1:100, Millipore, AB144P). Sections were rinsed in PBS then incubated for 2 hours at room temperature in blocking solution containing secondary antibodies ...
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bioRxiv - Bioengineering 2022Quote: ... pH 9.0) followed by blocking for 1 hour at room temperature with 20v/v% goat serum (Sigma-Aldrich) in PBS ...
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bioRxiv - Biophysics 2022Quote: ... for 30 min and blocked for at least 2 h with a blocking buffer containing 1% BSA (Sigma), 3% donkey serum (Millipore) ...
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bioRxiv - Molecular Biology 2019Quote: ... Free thiol groups were blocked with blocking solution (250 mM HEPES, pH 7.7, 1 mM EDTA, 0.1 mM neocuproine [Sigma, P9599] ...
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bioRxiv - Bioengineering 2020Quote: ... Permeabilization and blocking solutions were made with 0.3% Triton X (PanREAC AppliChem) and 0.5% BSA solution (Sigma-Aldrich) for 30min ...
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bioRxiv - Neuroscience 2020Quote: ... followed by blocking for 20 min at RT in 5% normal donkey serum (Sigma, Cat N° D9663-10ML) in 0.1% PBS-Triton X-100 ...
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bioRxiv - Neuroscience 2021Quote: The brain slices were incubated in blocking solution containing 1% (w/v) bovine serum albumin (Sigma, Cat#A9418), 5% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... washed 3x in PBS followed by permeabilization and blocking in PBS containing 0.1% Triton X-100 (EMD Millipore) and 20% fetal calf serum (TPBS-FCS ...
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bioRxiv - Cancer Biology 2020Quote: ... After treating with 0.2% Triton and blocking with 1% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, USA), 3ug/ml anti-BrdU primary antibody was added O/N ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were incubated in blocking solution consisting of 5% v/w Skim Milk Powder (Sigma-Aldrich #70166-500G) in PBS with 0.05% Tween 20 (PBST ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were then blocked for one hour in blocking solution (DPBS containing 10% goat serum (Sigma Aldrich, G9023), and 0.3% Triton X-100) ...
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bioRxiv - Developmental Biology 2020Quote: ... cells are washed 3 times with PBS and blocking solution is added (Bovine Serum Albumin, BSA. Sigma; A7906) at 3% in PBS for at least 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed again with blocking solution at RT and DNA was counterstained with DAPI (Sigma-Aldrich, D9564). For EdU staining ...
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bioRxiv - Neuroscience 2022Quote: ... and incubation in blocking solution (3% bovine serum albumin, 0.5% Triton x-100, Sigma, St. Louis, MO, USA) in 0.1M PBS ...
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bioRxiv - Neuroscience 2022Quote: ... sections were first treated with a blocking solution containing 10% normal donkey serum (NDS, Sigma-Aldrich: S30-M) or 10% normal goat serum (NGS ...
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bioRxiv - Bioengineering 2022Quote: ... After blocking for 1 h by adding a drop of 3 % bovine serum albumin (A9418, Sigma-Aldrich, USA) onto exposed tissue ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with TSA blocking reagent containing peroxidase-conjugated sheep anti-DIG antibody (1:1,000, Sigma, 11207733910) at room temperature for 2 h ...