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Citations for Millipore Sigma :
1151 - 1200 of 2420 citations for RGS4 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... This was followed by blocking for 1 hour in 1% human serum albumin (HSA; Sigma-Aldrich) and 0.1% Triton X-100 dissolved in TBS ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a blocking step for 1h with 5% normal donkey serum (NDS) (D9663, Sigma Aldrich) in 0.1% PBS-Triton X-100 (PBS-T) ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2.5 mM of the maturation-blocking phosphodiesterase 3 inhibitor milrinone (2.5mM; Sigma Milipore M4659). GV (Germinal-Vesicle ...
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bioRxiv - Developmental Biology 2022Quote: ... The embryos were next transferred to blocking solution (5% donkey serum (Sigma-Aldrich, Cat No. D9663), 1%BSA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... and conjugate antibodies were all diluted using the blocking solution (10% goat serum in PBS, Sigma). The primary antibodies used were γMLC2 rabbit Ab 1:100 (3672 ...
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bioRxiv - Biophysics 2022Quote: ... Cell were washed and then incubated in the blocking buffer (3% BSA (A9418, by Sigma-Aldrich) + 0.1 mg/mL of sheared salmon sperm DNA (AM9680 ...
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TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring genebioRxiv - Genetics 2022Quote: ... Sections were washed with 1X PBS and incubated in blocking solution: 2% BSA (Sigma Aldrich, A2153), 0.2% Triton X-100 (Amresco ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated for 1 h with a blocking solution containing 3% BSA (BP9703-100; Sigma-Aldrich). Cells were incubated for 1 h with anti-ACBD3 and anti-GM130 primary antibodies followed by 1 h incubation with appropriate Alexa Fluor Dyes ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by incubation with blocking solution (1% BSA, 3% Donkey Serum [Sigma: D9663], 0.1% (v/v) Tween in PBS ...
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bioRxiv - Microbiology 2023Quote: ... blocked for one hour in blocking buffer (3% goat serum and 3% BSA in PBS (Sigma)) and incubated with pneumococcal antisera Pool Q (SSI Diagnostica ...
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bioRxiv - Cell Biology 2023Quote: ... slides were incubated for 1h at RT in blocking buffer containing 10% goat serum (Sigma, G9023) with 0.03 % triton X-100 in PBS and 1:50 Affini Pure Fab Fragment Donkey Anti-Mouse IgG (Jackson Immuno Research Laboratories ...
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bioRxiv - Immunology 2024Quote: ... The slices were first incubated in a blocking solution of PBS-Triton 0.1% (X100 Sigma-Aldrich), 5% BSA (A3294 Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... Blocking and permeabilization of the cells were performed by 10 % FBS with 0.1 % TritonX-100 (Sigma) in PBS (1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... Blocking and antibody incubation was done using 3% bovine serum albumin (BSA) (Sigma-Aldrich A7906-10G) and 0.3% TritonX (Sigma-Aldrich T8787 ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated overnight with primary antibodies diluted in Odyssey blocking buffer and 0.2% Tween20 (Sigma-Aldrich) at 4°C ...
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bioRxiv - Plant Biology 2024Quote: Blocking buffer: 5% bovine serum albumin (BSA) (Sigma-Aldrich, Cat. No. A8577, Saint Louis, MO, USA) in PBST (0.1% Tween-20)
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bioRxiv - Bioengineering 2024Quote: ... Permeabilization and blocking were performed for two hours in PBS containing 0.125% Triton X (Sigma-Aldrich), 0.5% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... both in blocking solution and detection using the Supersensitive TMB substrate (3,3’,5,5’-tetramethylbenzidine, Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Blocking was performed with 5% Normal Donkey serum (NDS) and 0.3% Triton X-100 (Sigma-Aldrich) in 0.1M PB solution ...
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bioRxiv - Bioengineering 2024Quote: ... Blocking and permeabilization steps were done with 1% BSA and 0.5 % cold fish gelatin (Sigma-Aldrich) dissolved in Tris + 0.1% Triton solution ...
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bioRxiv - Cancer Biology 2024Quote: ... PDOTS side channels culture media was replaced with a blocking solution of 2% BSA (Sigma, A7906) in PBS for 30 min in room temp ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked for 1 h at room temperature in 1 x casein blocking buffer (Sigma) and immunoblotted using primary antibody to Claudin-2 (1:500 dilution ...
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bioRxiv - Plant Biology 2021Quote: ... washed 5 times and then eluted with 3xFLAG peptide ([0.1 mg/mL]; Sigma, Cat# F4799). The crosslinking was reversed overnight at 65 °C and purified DNA (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 mM NaCl) and eluted with 300 ng/L of FLAG peptide (#F4799, Sigma-Aldrich) for four hours under moderate agitation 62 ...
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bioRxiv - Microbiology 2020Quote: ... bound proteins from the pooled washed resin were eluted with 100μg of FLAG peptide (Sigma) in FLAG wash buffer ...
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bioRxiv - Genomics 2020Quote: ... The beads were washed extensively and were eluted with 100 μg/ml 3xFLAG peptide (Sigma). The eluates were incubated with TRIzol reagent followed by isopropanol precipitation and DNase I digestion (Fermentas) ...
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bioRxiv - Molecular Biology 2021Quote: ... The HA-tagged PGBD1 was eluted by 500 μl 100 μg/ml HA-peptide (Sigma) in 100 mMNaCl ...
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bioRxiv - Developmental Biology 2019Quote: ... Peptides were dissolved in a small amount of 0.1% trifluoroacetic acid (302031, Sigma-Aldrich; TFA), followed by purification with the use of ZipTip (MA01821 ...
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bioRxiv - Biochemistry 2019Quote: ... The native proteins without signal peptides were expressed in BL21(DE3) Gold competent cells (Novagen). The gene encoding the LtgA deletion mutant lacking the alpha helix 503(ATAREIAGKIGMD)513 was chemically synthesized by ProteoGenix ...
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bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). All immunoprecipitations were performed on three independent biological replicates.
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bioRxiv - Biochemistry 2020Quote: ... 100 mM NaCl) and eluted with 50 μg/ml (final concentration) of 3xFLAG peptide (Sigma) in TGE ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in 40 µl lysis buffer + β-mercaptoethanol and 0.5 mg/mL 3xFLAG-peptide (Sigma) and incubated 30 minutes at 4°C to dissociate proteins bound to the FLAG-beads ...
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bioRxiv - Biochemistry 2020Quote: ... The mRNA-cyclic peptide libraries were eluted with 600 µL of 0.1% DEPC (Millipore Sigma) water containing 1 mM DTT ...
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bioRxiv - Biochemistry 2020Quote: ... The dried peptide films were dissolved in 200 μL Dimethyl sulfoxide (DMSO) >99.9% (Sigma-Aldrich), vortex for 1 min and sonicated for 1 min ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant INO80 complex was eluted with buffer B containing 1.6 mg Flag Peptide (Sigma-Aldrich). Anion exchange chromatography (MonoQ 5/50 GL ...
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bioRxiv - Neuroscience 2020Quote: ... a synthetic opioid peptide with high MOR specificity and clonidine HCl (Sigma, St Louis, MO). These compounds were aliquoted and concentrated using a Speed Vac and stored at −20°C before experimental procedures ...
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bioRxiv - Physiology 2021Quote: ... and 3X FLAG® Peptide (F4799) were obtained from Sigma Aldrich (St-Louis, Missouri, USA). The PKC inhibitor Gö6983 (ab144414 ...
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bioRxiv - Biochemistry 2021Quote: ... The MERS-CoV peptide was labeled with sub-stoichiometric FITC (Sigma-Aldrich, St. Louis, Missouri) in a basic HEPES buffer (pH 8.2 ...
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bioRxiv - Microbiology 2020Quote: ... the sequence encoding mature proteins without the signal peptide was cloned into pET-14b (+) (Novagen) to generate the expression vector pET14b-6xHis-BcCrh1 ...
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bioRxiv - Systems Biology 2020Quote: ... The dried peptides were reconstituted in 60 μL of 0.1% trifluroacetic acid (TFA) (Sigma-Aldrich). Peptides were then fractionated using SCX StageTip method as described earlier [23] ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins were eluted two times with 600 μL 250ug/mL 3xFLAG peptides (SIGMA, F4799) in TBS [50mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: Anti-FLAG-M2 affinity agarose gel and 3X FLAG peptide were obtained from Sigma Aldrich, n-dodecyl β-D-maltoside (DDM ...
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bioRxiv - Biophysics 2020Quote: ... The remaining FLAG beads were incubated with 150 ng/μL 3X FLAG peptide (Millipore-Sigma) in kinase assay buffer (100 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The remaining FLAG beads were incubated with 150 ng/μL 3X FLAG peptide (Millipore-Sigma) in kinase assay buffer (100 mM Tris pH 7.5 ...
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bioRxiv - Systems Biology 2022Quote: ... Purification and concentration of peptides was performed using C18 Zip Tip Solid Phase Extraction (Millipore). Peptides recovered from in-gel digestion processing were reconstituted into a final concentration of 0.5μg/μL of 2% ACN ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plasma insulin and plasma c-peptide were measured using radioimmunoassay (EMD Sigma, Cat #: RI-13K) and bead immunoassay (Luminex ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-PaIAP (rabbit, dilution 1:2500, peptide: [H]-KAENQKARFSDVHGC-[OH], Sigma-Genosys, Haverhill, United Kingdom), anti-PaMIC10 (rabbit ...
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bioRxiv - Systems Biology 2022Quote: ... Purification and concentration of peptides was performed using C18 Zip Tip Solid Phase Extraction (Millipore). The peptides recovered were reconstituted into a final concentration of 0.5μg/μL of 2% ACN ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged proteins were eluted by 60 μl of 3x FLAG® Peptide (Sigma F4799) (diluted in Tris-buffered saline (TBS ...
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bioRxiv - Neuroscience 2021Quote: ... Peptides were dissolved in 80% ACN/5%TFA with 1 M glycolic acid (Sigma-Aldrich) and incubated with 0.6 mg titanium dioxide (TiO2 ...