Labshake search
Citations for Millipore Sigma :
1351 - 1400 of 3948 citations for IL 23R Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2020Quote: ... Tumor tissue was dissected into fragments of 1-2mm3 and frozen in 90% fetal calf serum (FCS, Sigma) and 10% dimethyl sulfoxide (DMSO ...
-
bioRxiv - Neuroscience 2022Quote: ... Medium supplemented with EV-depleted FCS and antibiotics was subsequently filtered using 0.22 and a 0.1 µM filter-sterilization devices (Millipore). For EV isolation ...
-
bioRxiv - Immunology 2019Quote: ... Monocytes were cultured in RPMI-1640 medium supplemented with 10% FCS and 1% sodium pyruvate (all from Sigma) in ultra-low attachment 6-well plates ...
-
bioRxiv - Immunology 2019Quote: ... cells were counted at d0 and 0.4x106 cells/ml were seeded in differentiation medium (RPMI supplemented with 2.5 % FCS, 0.5% dimethylformamide (DMF, D4551, Sigma), L-glutamine and antibiotics) ...
-
bioRxiv - Molecular Biology 2021Quote: Wildtype and ΔMETTL3 mES cells were maintained in DMEM supplemented with 15% FCS (ES-009-B, Millipore Sigma), 1X non-essential amino acids (11-140-050 ...
-
bioRxiv - Genetics 2021Quote: ... They were transferred to digestion medium (HBSS with Ca2+ Mg2+, FCS 2%, DNase I 0.5mg/mL (Sigma-Aldrich) and collagenase IV 0.5mg/mL (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2020Quote: ... The EV-containing eluates were pooled and supplemented with 10% heat-inactivated fetal calf serum (FCS; Sigma-Aldrich), or 0.1% BSA (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2021Quote: ... Cells were washed and resuspended in PBS containing 0.5 % FCS and subjected to FACS analysis (Guava EasyCyte, Millipore).
-
bioRxiv - Cell Biology 2023Quote: ... Sorted single cells were collected in ADF plus 10% FCS and 9 µM Rock inhibitor (Y-27632, Sigma) to prevent anoikis ...
-
bioRxiv - Cancer Biology 2023Quote: ... Immunoreactive bands were visualised on Licor Odyssey Fc (Cambridge, UK) using enhanced chemiluminescence (ECL, Merck Millipore, Darmstadt, Germany). β-actin was used a loading control.
-
bioRxiv - Immunology 2023Quote: ... Surface staining was performed for 30 min at 4 °C in PBS supplemented with 2% FCS (Sigma-Aldrich) and 0.01% azide (Sigma-Aldrich ...
-
bioRxiv - Immunology 2023Quote: ... 2% FCS and 50 μg/ml Lipopolysaccharide neutralizing agent Polymyxin B sulfate (cat # 1405-20-5; Sigma-Aldrich) for 30 min at 37°C while shaking by 750 rpm on a heating block ...
-
bioRxiv - Genetics 2023Quote: ... supplemented with 10% fetal calf serum (FCS, Capricorn, FBS-12A) and 1% penicillin/streptomycin (P/S, Sigma, P0781). The cultured fibroblasts were incubated at 37°C with 5% CO2.
-
bioRxiv - Cell Biology 2024Quote: ... Serum-cultured cells were grown on 0.1% gelatin-coated plates in Serum ES media (GMEM, 10% FCS (Sigma), 2mM Glutamax ...
-
bioRxiv - Genomics 2024Quote: ... 20% FCS (Autogen Bioclear, Calne, UK) and 1,000 U/ml of leukemia inhibitory factor (ESGRO-LIF, Chemicon/Millipore). For the Ring1B conditional deletion ...
-
bioRxiv - Bioengineering 2019Quote: ... Milliplex Human Angiogenesis / Growth Factor kit Discovery Assay® and the Human Cytokine Array / Chemokine Array (Millipore, St. Charles, MO, USA)] ...
-
bioRxiv - Bioengineering 2021Quote: ... Mature human white-like adipocytes were transfected in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522) to mimic the subcutaneous tissue environment ...
-
bioRxiv - Immunology 2020Quote: ... Plates were washed 6 times with washing buffer and then incubated with anti-human IgG (Jackson Immuno Research 109-036-088) or anti-human IgA (Sigma A0295) secondary antibody conjugated to horseradish peroxidase (HRP ...
-
bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 48 hours at 4°C with the following primary antibodies: anti-GBA (1:500; human 8E4; human and rodent G4171, Sigma-Aldrich), anti-human α-synuclein (syn211 ...
-
bioRxiv - Cell Biology 2021Quote: ... Transfection for mature human white-like adipocytes was performed in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522). Both HeLa cells and mature human white like adipocytes were transfected with LNPs at a final mRNA concentration of 1.25μg/mL ...
-
bioRxiv - Pathology 2023Quote: ... adiponectin levels were measured by enzyme-linked immunosorbent assays (Human Chemerin ELISA kit, Biovendor, Brno, Czech Republic; Human Resistin ELISA kit, Millipore Sigma; Human Total Adiponectin/Acrp30 Quantikine ELISA Kit ...
-
bioRxiv - Bioengineering 2024Quote: ... The immortalized Podo/Tert256 human podocyte hTERT cell line (Evercyte) was expanded in flasks coated with 6 µg/cm2 human collagen I (Sigma, C7774) at 37°C in MCDB131 basal media (Gibco ...
-
bioRxiv - Microbiology 2024Quote: ... The human ORF sequence encoding for GSDMD was obtained from the Viral Vector Facility (LUMC) human ORF (cDNA) library from Sigma-Aldrich. The gene ORF was obtained in pDONR223 entry vectors and amplified by PCR with overhang primers to introduce partial sequences of an N-terminal FLAG-tag and C-terminal Myc-tag ...
-
bioRxiv - Cell Biology 2024Quote: Human umbilical vein endothelial cells (HUVECs) were isolated from human umbilical cords using collagenase type I (Sigma-Aldrich, 400 U/mL) to separate the cell layer surrounding the vein lumen ...
-
bioRxiv - Molecular Biology 2021Quote: ... ACC 57), K562 (human, DSMZ Germany ACC 10), and Molm-13 (human, DSMZ Germany ACC 554) were cultured in RPMI1640 (Sigma-Aldrich R8758) supplemented with 10 % heat-inactivated FCS and 1 % penicillin streptomycin mixture ...
-
bioRxiv - Biochemistry 2019Quote: ... the GAP-334 region of human p120GAP (residues 715-1047) and the SOScat domain of human SOS1 (residues 564-1049) are cloned into pET15b (Novagen / EMD Biosciences) with an N-terminal polyhistidine (His ...
-
bioRxiv - Cancer Biology 2019Quote: ... ECM proteins (collagen I and natural mouse laminin were purchased from Life Technologies, human plasma fibronectin from Millipore, human collagen IV from Sigma-Aldrich, recombinant human tenascin C ...
-
bioRxiv - Immunology 2023Quote: ... Immune mediators in cell culture supernatant following CD3/CD28 bead stimulation were measured using a human premixed CD8+ T Cell Human 17-plex panel (Millipore, Temecula, California). All Luminex assays were analyzed using a MAGPIX Instrument and xPONENT software (Luminex ...
-
bioRxiv - Biochemistry 2021Quote: ... cells were cultured for at least six days in RPMI 1640 Medium supplemented with 10% dialyzed FCS (Sigma Aldrich), containing either “heavy” lysine (40 g/l ...
-
bioRxiv - Biophysics 2020Quote: ... Cytochalasin D (from Zygosporium masonii) as well as fetal calf serum (FCS) were from Sigma Aldrich (Merck KGaA, Germany). DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The HE-Fc containing fractions were dialyzed in PBS and concentrated using 30kD Amicon Ultra-15 filters (EMD Millipore). Purified proteins were observed by SDS gel migration as well as anti-human IgG Fc probing by Western ...
-
bioRxiv - Developmental Biology 2019Quote: ... media was replaced with DMEM/F12 containing 100ng/ml Activin A and 0.2% foetal calf serum (FCS, Sigma-Aldrich) for a further 24 hours ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were grown in DMEM (gibco) with 1 g/mL glucose (MCEC) or 4.5 g/mL (HUVEC, HeLa, MCF-7) containing 10% FCS (Sigma), 1% penicillin (10000 Units/ml ...
-
bioRxiv - Bioengineering 2021Quote: ... Cells were seeded at 1E6 cells/ml in MEM-Alpha (Biological Industries) supplemented with 10% Heat Inactivated FCS (Sigma) and 50IU rhIL-2 (Peprotech).
-
bioRxiv - Cancer Biology 2020Quote: ... MDA-MB-231 cells were cultured in DMEM medium (Pan-Biotech, Germany) supplemented with 10% FCS (Sigma-Aldrich, Germany), 2 mM L-glutamine (Pan-Biotech ...
-
bioRxiv - Biophysics 2020Quote: All used BMPs and extracellular domains (ECD) of the BMPR-FC chimeras were bought from Sigma Aldrich (Missouri, USA) and R&D systems (Minnesota ...
-
bioRxiv - Cell Biology 2020Quote: ... the colon pieces were digested in RPMI-1640 medium containing 20% FCS and 100CU/mL collagenase IV (Sigma-Aldrich). Single cells were separated from the remaining tissue by filtration through 40 µm cell strainers and washed with RPMI-1640 thereafter ...
-
bioRxiv - Synthetic Biology 2022Quote: ... were used in this work: The perfluorinated amine Fluorinert™ FC-40 was purchased from Sigma-Aldrich (Overijse, Belgium), while the perfluorinated ether FC-770 and the perfluorinated amine FC-3283 were obtained from Acros Organics (Geel ...
-
bioRxiv - Immunology 2020Quote: ... Intestines were then incubated with 20 mL RPMI + 10% FCS +1% PS + 2.5 U/mL Collagenase VIII (Sigma-Aldrich) + 2 U/mL DNAse I (Roche) ...
-
bioRxiv - Cancer Biology 2019Quote: ... cell pellets were resuspended with 10% FCS and fixed by adding an equal volume of 4% formaldehyde (Sigma-Aldrich) and mixed for 10 min at room temperature while shaking ...
-
bioRxiv - Microbiology 2019Quote: ... washed cells were resuspended in incubation buffer (IB; Hank’s balanced salt solution, 2% dialysed FCS, 1x RPMI Amino Acids Solution (Sigma), 2 mM L-glutamine ...
-
bioRxiv - Microbiology 2021Quote: ... Purified ACE2-Fc was concentrated and buffer exchanged into PBS by a 50-kDa MWCO ultra centrifugal filter (Millipore).
-
bioRxiv - Immunology 2019Quote: ... resuspended in RPMI-10% FBS containing 40ng/mL CTLA4-Ig (CD152/Fc chimera, non-cytolytic, from mouse, Sigma C4358), plated in 24-well culture plates at a density of 2 x 106 cells/well and cultured for an additional 24 hours (BMDCCTLA4-Ig) ...
-
bioRxiv - Immunology 2019Quote: ... Intestines were then incubated with 20 mL RPMI + 10% FCS +1% PS + 2.5 U/mL Collagenase VIII (Sigma-Aldrich) + 2 U/mL DNAse I (Roche) ...
-
bioRxiv - Molecular Biology 2019Quote: ... were cultured in Eagle’s Minimum Essential Medium (ATCC, Middlesex, UK) supplemented with 10% FCS and 0.8 mg/ml geneticin (Sigma-Aldrich). All cells were cultured at 37°C and 5% CO2 in humidified incubators and were free of mycoplasma contamination.
-
bioRxiv - Immunology 2021Quote: ... cells were incubated in medium containing 2% FCS with or without the PAR2 negative allosteric modulator (AZ3451, Sigma Aldrich) or a NF-κβ inhibitor (Bay11-0782 ...
-
bioRxiv - Microbiology 2020Quote: ... FMC Biopolymer (90) in DMEM (containing 2% FCS and antibiotics) and serial dilutions of 5-FU (F6627, Sigma-Aldrich) or Ribavirin (R9644 ...
-
bioRxiv - Microbiology 2020Quote: ... The mice were boosted subcutaneously (s.c.) at day 8 with 50 μg of RBD-Fc plus Freund’s Adjuvant Complete (Sigma, USA) and at day 13 with 50 μg of RBD-Fc plus Titermax adjuvant (Sigma) ...
-
bioRxiv - Immunology 2020Quote: ... Digestion of the colon tissue was performed in RPMI/10% FCS containing 0.4mg/ml type VIII collagenase (Sigma Aldrich) and 40μg/ml DNase I (Roche ...
-
bioRxiv - Microbiology 2022Quote: ... Inocula were removed and replaced with DMEM containing 1% FCS and a semi-solid overlay consisting of 1.5% carboxymethyl cellulose (SIGMA). Cells were incubated for 72h ...