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Citations for Millipore Sigma :
1601 - 1650 of 3948 citations for IL 23R Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... The bottom oil was then removed and replaced with FC-40 fluorinated oil (Sigma-Aldrich, catalog no. 51142-49-5) with 5% (w/w ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse recombinant EphrinB1-Fc chimera (cat # E0653-200UG) and bovine serum albumin (cat #05470-1G) with purity >98 % were from Sigma. Alexa Fuor-594 BSA (cat #A13101) ...
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bioRxiv - Developmental Biology 2019Quote: ... in 400ul of a solution containing 1x PBS complemented with 10% FCS and 5ul Collagenase 10mg/ml (sterile filetered, C1764 Sigma). Fixation was done in a final concentration of 1% FA (w/o methanol ...
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bioRxiv - Developmental Biology 2019Quote: ... in 250ul of a solution containing 1x PBS complemented with 10% FCS and 5ul Collagenase 10mg/ml (sterile filtered, C1764 Sigma). Fixation was done in a final concentration of 2% PFA for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Cells migrated through the porous membrane into the lower transwell chamber containing supplemented DMEM/ 10% FCS and pro-migratory agent lysophosphatidic acid (LPA; 10 μM; Sigma) for 20 h ...
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bioRxiv - Immunology 2019Quote: ... were maintained in DMEM-Glutamax supplemented with 10% heat-inactivated FCS and 100 U/ml/100 mg/ml of PS and G418 (SIGMA) at 400 μg/ml ...
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bioRxiv - Immunology 2019Quote: ... Spleens and lymph nodes were cut into small pieces and digested in IMDM (2% FCS) containing collagenase type IA (1 mg/ml, Sigma) and DNase I (10 U/ml ...
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bioRxiv - Biophysics 2019Quote: ... fab-fragment solution consisting of 100 μg/ml F(ab’)2 fragments (anti-mouse IgG [Fc specific] antibody developed in goat; Sigma) in PBS that bind unspecifically to the hydrophobic surface (incubation time 5 min) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were grown in HMI-9 medium 53 supplemented with 10% fetal calf serum (FCS) (Sigma-Aldrich, St. Louis, USA) and incubated at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2019Quote: ... The collagen solution containing cells was perfused into the microfluidic device inlet at 150 μL/hour in a cold room (4ºC) while fluorocarbon oil (FC-40, Sigma-Aldrich) with 2% 008-fluoroSurfactant (RAN Biotechnologies ...
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bioRxiv - Microbiology 2021Quote: ... 293T cells (ATCC CRL-3216) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (PAN-Biotech) with 10% feral calf serum (FCS) (Sigma-Aldrich), 1% L-Glutamine (Sigma-Aldrich) ...
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bioRxiv - Physiology 2021Quote: ... EDL muscles were digested in Krebs solution (without FCS) containing 3 mg/ml collagenase type IV A (Sigma Aldrich, USA), gently bubbled with carbogen (95% O2 ...
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bioRxiv - Biophysics 2020Quote: ... the cells were incubated with a FCS-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days ...
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bioRxiv - Microbiology 2022Quote: ... Single cell suspensions were incubated with Fc block (Miltentyi Biotec) in FACS buffer (1% BSA, 2mM EDTA in DPBS, Sigma), followed by staining with anti-CD11b-PerCP-Cy5.5 (#45-0112-82 ...
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bioRxiv - Immunology 2021Quote: ... Lung & spleen cells were diluted to 1×106cells/ml before incubating with 50 μg/ml SARS-CoV-2 purified spike antigen for 24 h in media (RPMI-1640, 10% FCS, 100U/ml penicillin/streptomycin, 5%NEAA, L-glutamine and HEPES, 0.05 mM β-mercaptoethanol (SIGMA)) ...
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bioRxiv - Cell Biology 2021Quote: ... then added to cells and incubated for 2 hours before discarding and adding a 2.4% (w/v) microcrystalline cellulose and 2% FCS (mixed 1:1) overlay (Sigma-Aldrich). The assay was incubated at 37°C and 5% CO2 for 70 hours then fixed in 4% PFA for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... flow channels of the Drop-seq chip were subjected to a hydrophobicity treatment using 1H,1H,2H,2H-Perfluorodecyltrichlorosilane (in 2% v/v in FC-40 oil; Alfa Aeser/Sigma). After 5 min of treatment ...
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bioRxiv - Immunology 2022Quote: ... and spike protein-bound antibodies were detected using an Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich, #A2429) at a 1:4000 dilution in blocking buffer ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... For capture of antibodies and Fc-Fusions a CM5-S-Series sensor chip (Cytiva) was functionalized with 2500RU recombinant Protein A (Sigma) using EDC/NHS Coupling Kit (Biacore/Cytiva BR-1000-50 ...
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bioRxiv - Immunology 2019Quote: The large intestine was opened longitudinally with blunt ended scissors and cut into 0.5cm segments before washing in 2% foetal calf serum (FCS) in Hank’s Balanced Salt Solution (HBSS) (Sigma, Life Sciences). To remove epithelial cells ...
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bioRxiv - Physiology 2020Quote: ... Systemic levels of vascular markers were evaluated at the end of the Fc-GDF15 and vehicle treatment of obese ZSF1 rats by using multiplex MILLIplex kits (Millipore). The rat vascular injury panels included VEGF ...
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bioRxiv - Cancer Biology 2019Quote: ... and resuspended in 50 to 80 µl FC buffer containing 0.1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich). Primary tumor samples were stained with CD45-APC-Cy7 (Biolegend ...
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bioRxiv - Molecular Biology 2021Quote: ... with 8% fetal calf serum (FCS; Bodinco) supplemented with 100 IU/ml of penicillin and 100 µg/ml of streptomycin (Sigma) and 2 mM L-Glutamine (PAA Laboratories) ...
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bioRxiv - Molecular Biology 2019Quote: ... BT-474 and MDA-MB-453 cells were cultivated in DMEM medium (Pan-Biotech) supplemented with 10% FCS (Sigma-Aldrich), 2 mM L-Glutamine (Pan-Biotech ...
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bioRxiv - Cell Biology 2020Quote: ... cells were matched-paired and incubated in CO2-free conditions in L-15 medium (supplied with 10% FCS 2mM L-glutamine and 10μg/ml Pen/Strep) and LPS (Sigma-Aldrich) or CSF-1 (Miltenyi Biotec Australia Pte Ltd) ...
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bioRxiv - Microbiology 2020Quote: ... with 8% fetal calf serum (FCS; Bodinco) supplemented with 100 IU/ml of penicillin and 100 μg/ml of streptomycin (Sigma), and 2 mM L-Glutamine (PAA) ...
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bioRxiv - Immunology 2020Quote: ... 8-µm-thick tissue sections were rehydrated and Fc-blocked with normal mouse and rat serums in the presence of 0.3% Triton X100 (Sigma-Aldrich). Tissues were stained with F4/80-APC (Clone BM8 ...
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bioRxiv - Developmental Biology 2020Quote: Spinal cords were incubated at 38°C for 20 minutes with F12 medium supplemented with 5% FCS (F7524; Sigma-Aldrich), 20mM HEPES buffer (15630-049 ...
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bioRxiv - Microbiology 2020Quote: ... containing 4.5 g l−1 D-glucose and L-glutamine supplemented with 10 % fetal calf serum (FCS; Sigma Aldrich, USA), 100 U ml−1 penicillin and 100 μg ml−1 streptomycin (PAN Biotech ...
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bioRxiv - Genetics 2022Quote: ... and 10% heat-inactivated fetal calf serum (FCS) and stimulated with 25 μg/ml lipopolysaccharide (LPS) (Salmonella typhosa origin, Sigma). CD8 T cells were stimulated using anti-CD3 (10μg/ml plate-bound ...
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bioRxiv - Microbiology 2022Quote: ... then cells were replenished with DMEM containing 10% (v/v) FCS and 50 μg/ml gentamicin (Sigma Aldrich, cat# G1397) and incubated for 72 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... the culture medium was exchanged to medium without growth factors but supplemented with 1% fetal calf serum (FCS, Sigma Aldrich). The cells were differentiated for 5 days followed by fixation or preparation for WB or RNA isolation ...
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bioRxiv - Cell Biology 2023Quote: ... cells were grown for 3 days in phenol red-free media supplemented with 10% charcoal-stripped FCS (-E2) and subsequently treated with 100nM E2 (Sigma) for the indicated times.
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bioRxiv - Cancer Biology 2023Quote: ... 4T1 cells expressing shRNA to knock down Beclin 1 or Atg7 expression or control 4T1 cells expressing scrambled shRNA were treated with mouse rPD-1-Fc (1 μg/mL) for 30 minutes and subsequently incubated in medium containing doxorubicin (0.5 μM; Sigma #D1515) for one hour ...
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bioRxiv - Immunology 2023Quote: Cells were cultured in R10 medium, (RPMI 1640 containing 10% heat inactivated FCS, 50μM 2-mercaptoethanol with penicillin and streptomycin, (Sigma-Aldrich) and incubated at 37°C ...
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bioRxiv - Immunology 2023Quote: ... USA) cells and the respective sublines were maintained in RPMI-1640 medium containing 8% fetal calf serum (FCS) (Sigma, F7524), 2 mM L-glutamine ...
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bioRxiv - Immunology 2023Quote: ... 8129) cells were maintained in Dulbecco Modified Eagle Medium (DMEM) supplemented with 10% fetal calf serum (FCS, Sigma cat. F7524) and 100 U/ml of penicillin/streptomycin ...
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bioRxiv - Physiology 2023Quote: ... Supernatants from cells overexpressing Fc-tagged FGL1 proteins were collected after 5 days and supplemented with protease inhibitor cocktail (Sigma). Recombinant proteins were purified using Hitrap Protein A HP column on an ÄKTA pure chromatography system (GE healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... Following secondary antibodies at the indicated dilution were used for IF: Anti-rabbit IgG (Fc) TRITC (Sigma #SAB3700846, 1:500), Anti-mouse IgG (Fc ...
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bioRxiv - Biochemistry 2023Quote: ... the Siglec-7-Spy Tag-TEV-Fc was concentrated using an AMICON (Molecular Weight Cut Off 30 kDa – Merck Millipore) to approximately 0.15 mg/ml measured via NanoDrop as described above ...
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... cells were dissociated by incubating the pooled tissue in 250gl PBS supplemented with 10% fetal fetal calf serum (FCS) and 1 mg/ml collagenase (Sigma) for 45 minutes at 37° C with shaking at 750 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... Inocula were removed and replaced with DMEM containing 1% FCS with a semi-solid overlay consisting of 1.5% carboxymethyl cellulose (Sigma-Aldrich). Cells were incubated for 4-5 days ...
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bioRxiv - Immunology 2023Quote: ... Pellets were recovered in PBS 2% FCS and erythrocytes were removed using a red blood cell lysis buffer (Sigma-Aldrich) before being filtered onto a 40-µm cell strainer (Dutscher) ...
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bioRxiv - Immunology 2024Quote: ... the cells were stained for 30 min on ice in PBS containing 2% FCS and 0.1% sodium azide (cat. S2002, Sigma-Aldrich). To examine intracellular cytokine production ...
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bioRxiv - Immunology 2024Quote: ... All bacteria cultures were then centrifuged at 3,000 rpm for 10 min and the bacterial pellet was resuspended into 300μl of RPMI/5% FCS with gentamycin (Sigma Aldrich) (30 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... and all fractions containing IL-22 were concentrated using a 10 kDa centrifugal filter (Merck Millipore, USA).
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bioRxiv - Immunology 2021Quote: Cre-J2 immortalized IL-1β-/- BMDMs were passaged in DMEM containing 10% foetal bovine serum (FBS, Sigma), 50 U/ml penicillin and 50 μg/ml streptomycin (complete media ...
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bioRxiv - Developmental Biology 2022Quote: ... The IL-6 inhibitors LMT-28 and the Wnt/β-catenin inhibitor IWR-1 (I0161, SIGMA-ALDRICH) were both used at 10 μM.
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bioRxiv - Molecular Biology 2020Quote: ... IL) and Rat DIMT1 siRNA (Cat# NM_001106408 (SASI_Rn02_00218668) was from (Sigma Aldrich, St. Louis, Missouri, United States) Fluorescent probes were supplied by Molecular Probes (Rockford ...
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bioRxiv - Biochemistry 2022Quote: ... supplemented with Halt protease inhibitor (Pierce, Rockford, IL, USA) and benzonase (Millipore Sigma, St. Louis, MO, USA) for 3-5 mins at RT and spun at max speed (14,800 rpm ...