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Citations for Millipore Sigma :
1351 - 1400 of 2153 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the same samples were incubated for 2.5 h at 37°C with protein synthesis inhibitor cycloheximide (10 μg/ml) (CHX; Sigma-Aldrich), of which experimental condition was optimized in our previous reports 18,21 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then pre-incubated for 1 h at RT in incubation buffer: 5 % normal donkey serum (EMD Millipore, USA) and 0.2 % Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... Leucocytes at 1×106 were then kept in short period culture (4 h) with Brefeldin A (10 μg) (Sigma-Aldrich), 5 μg PMA (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... PBST) for 1 h at RT followed by incubating with primary antibodies (anti-PV, 1:1000-2000, P3088, Sigma-Aldrich) in 3% NGS in PBST at 4 °C overnight ...
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bioRxiv - Cell Biology 2020Quote: h-TERT-immortalized Retinal Pigment Epithelial (RPE-1, ATCC, CRL-4000, USA) cells were grown in DMEM/F12 (Sigma Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... Linear pST76-A plasmid was ligated with the PCR fragment using T4 ligase enzyme overnight at 16 °C followed by heat inactivation at 65 °C for 10 minutes and dialysis for 1 h on a 50 nm pore-sized membrane (Millipore). The ligation product was transformed into E ...
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bioRxiv - Microbiology 2020Quote: ... parasites at 32 hpi and 38 hpi were cultured at 37 °C or 40 °C for 6 h in the presence or absence of 20 μM Wortmannin (Selleckchem, Houston, TX) or 40 μM LY294002 (Sigma). The cells were subsequently washed and resuspended in complete medium (1% hematocrit) ...
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bioRxiv - Microbiology 2020Quote: ... transferred to DMEM (1000 mg/l) and media glucose concentration was measured over a 24 h period using a glucose assay kit (Sigma). Glucose levels were expressed relative to media collected from worms which received naïve IgG (negative control) ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was harvested after 48 and 72 h of culture and concentrated using an Amicon filter (100K NMWL, Millipore).
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bioRxiv - Cell Biology 2020Quote: ... 3d, e, Fig. 4b) or 15kPa gels (Fig. 4g, h) were treated with 15µM of Blebbistatin or DMSO (Sigma-Aldrich) in ENR medium for 3h ...
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bioRxiv - Microbiology 2021Quote: ... 0.4 % SDS [pH 8.1]) was added and samples were incubated for 2.5 h with magnetic FLAG® beads (Sigma-Aldrich) in a rotation wheel at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at room temperature followed by alkylation of cysteine residue side chain thiol groups with iodoacetamide (Sigma-Aldrich) at approximately 17 mM final concentration for 30 min at room temperature in the dark ...
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bioRxiv - Biophysics 2021Quote: ... The virus-containing supernatant was concentrated by ultracentrifugation in a SW-28 rotor (25,000 rpm, 2 h, 4°C) using OptiPrep density gradient medium (Sigma-Aldrich). Part of the supernatant containing pelleted viruses was collected ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µM rotenone for 60 min or for 2 h with 0.5 mM L-leucyl-L-leucine methyl ester (LLOMe; L7393, Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... incubated for 1 h with secondary antibody and mounted with antifading Polyvinyl alcohol mounting medium with DABCO® (Sigma, #10981) after washing three times with PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... were cultured for 1 h in culture medium not containing foetal bovine serum but supplemented with 0.5% bovine serum albumin (Sigma-Aldrich), in the presence or absence of 10 ng/mL TG-1801 +/- U2 combination ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.3% Triton X-100 in 0.01 M PBS for 1 h at room temperature and then in chicken anti-GFAP (1:200; Millipore, AB5541) or rabbit IL-1β (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... blots were blocked for 1 h and incubated overnight at 4 °C with anti-Amyloid fibrils (OC, 1:5000, Merck-Millipore), anti-prefibrillar oligomers (A11 ...
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bioRxiv - Immunology 2022Quote: ... Day3 stimulated primary B cells were arrested in metaphase by a 1-h treatment with 0.1 μg/ml demecolcine (Sigma, D1925), treated with 0.075 M KCl ...
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bioRxiv - Biochemistry 2022Quote: ... the protein samples were reduced with 20 mmol/L dithiothreitol at 37 °C for 1 h and alkylated with 50 mmol/L iodoacetamide (IAA, Sigma) for 45 min in the dark ...
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bioRxiv - Bioengineering 2022Quote: ... samples were incubated for 1 h with 0.1 μg/ml DAPI and 50 pmol Atto 488-conjugated Phalloidin (49409, Sigma-Aldrich). Samples were washed and imaged in PBS and z-stacks were acquired with a confocal laser scanning microscope (Leica TCS SP8X ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 FlpIn mycBirA*FOPNL and HEK293 FlpIn mycBirA* cells were incubated for 24 h with 50 μM biotin (Sigma-Aldrich) in the presence or absence of 0.1 μg/ml tetracycline.
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bioRxiv - Plant Biology 2022Quote: ... and sterilized for 3 h in a mixture of bleach (30 mL) and concentrated hydrochloric acid (1.5 mL, Sigma-Aldrich). The pgb1-1 mutant lacks ethylene-induced PGB1 expression and lower hypoxia induced PGB1 expression (relative to wild-type ...
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bioRxiv - Physiology 2022Quote: ... Cells were incubated in the presence of serum-free DMEM-F12 medium containing 0.1% bovine serum albumin (BSA) and antibiotics for 24 h prior to treatment with 10ng/mL T3 (Sigma-Aldrich), 50 μg/mL Ascorbic Acid and/or 10mM β-Glycerol phosphate (BG) ...
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bioRxiv - Neuroscience 2022Quote: ... Lysolecithin (LPC)-induced demyelination experiments were carried out in cerebellar slices from P11 animals at day 7 in vitro by incubation for 16 h with 0.5 mg/ml LPC (Sigma-Aldrich) (Birgbauer et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged for 5 min at 20 000 x g and supernatants were immunoprecipitated for 1 h rotating at 4°C with mouse anti-GFP antibody (1 µg antibody per sample, 11814460001 Merck/Sigma) and 20 µl DynabeadsTM Protein G (10004D ...
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bioRxiv - Cancer Biology 2022Quote: ... Virus-containing medium was collected 48 and 72 h after transfection and was then added to cells supplemented with 8 mg/mL polybrene (TR-1003; Sigma) for lentiviral transduction ...
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bioRxiv - Genomics 2022Quote: ... The mycelium was washed in SMC and incubated for 4 h at 37 °C in lysing enzymes from Trichoderma harzianum (Sigma) dissolved in SMC ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were plated (2 ×106 cells per 100 mm plate) and grown for 24 h before transfection with Polyethylenimine (PEI, Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and A549 cells were transfected for 24 h using 4 µg/mL polybrene (H9268, Sigma-Aldrich, St. Louis, MO, USA). Puromycin selection (0.5–1.5 µg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... and treated for 4 h at 37°C in a polymerase solution containing fluorescent nucleotides as described (Sigma-Aldrich, Duolink). Samples were then washed 2x with buffer B (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was washed three times with 1 X TBST and incubated for 1 h with 1:10,000 diluted secondary antibody (Anti-Rabbit IgG peroxidase, Sigma-Aldrich) at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... adult DN mutant and Ctrl sibling zebrafish were bathed for 24 h in the presence of 5 μM 4-HT (H7904; Sigma) made from a 10 mM stock solution dissolved in 100% ethanol at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Mock- and virus-infected cells were harvested at 16 h post infection and stored at −80°C in RNALater (Sigma) until RNA extraction ...
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bioRxiv - Microbiology 2020Quote: ... incubated for 2 h with horseradish peroxidase (HRP)-conjugated secondary antibody (12-341 Rabbit anti-chicken RAC-HRP, Sigma-Aldrich) diluted 1:15000 in BSA-TBST ...
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bioRxiv - Neuroscience 2020Quote: ... then blocked with 3 % BSA in PBS for 1 h at room temperature and incubated with primary antibodies (mouse anti-GFAP, Sigma, 1:500; mouse anti-NeuN, Millipore, 1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... then blocked with 3 % BSA in PBS for 1 h at room temperature and incubated with primary antibodies (mouse anti-GFAP, Sigma, 1:500 ...
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bioRxiv - Microbiology 2020Quote: ... supernatant was collected in clean microcentrifuge tube and incubated 2 h at 4°C with a-flag mouse antibody (Sigma), following 1 h incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore) ...
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bioRxiv - Microbiology 2020Quote: ... then blocked for 1 h with 5% w/v milk in TBS and incubated for 1 h with primary antibodies a-flag 1:4000 (Sigma) or a-myc 1:4000 (Cell Signalling) ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were then incubated for 1 h with secondary antibody a-mouse HRP conjugate 1:10000 (Cell Signalling) and chemiluminescent substrate ultra (Millipore) was applied for 5 min before camera detection in Sapphire Biomolecular Imager (Azure Biosystems) ...
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bioRxiv - Molecular Biology 2020Quote: ... The primary antibody was then replaced with blocking solution containing 5 μg/mL bisBenzimide H 33342 trihydrochloride (Sigma-Aldrich, B2261) and goat anti-mouse Alexa Fluor 488 secondary antibody (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... and then with Laminin from Engelbreth-Holm-Swarm murine sarcome basement membrane (1.2 μg/mL in 1xPBS, 1 h at 37 °C, Sigma-Aldrich). They were passaged every two to three days in an appropriate ratio ...
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bioRxiv - Neuroscience 2020Quote: ... washed 3 times with PBS and incubated for 2 h with blocking and permeabilization buffer (1% BSA, 5% goat serum, 0.15% saponin (Sigma Aldrich)) ...
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bioRxiv - Immunology 2020Quote: ... Medium was harvested 72 h post transfection and filtered using 0.22 µm stericup-GP vacuum filtration system (Millipore, Burlington, MA). The supernatant from pDNA transfected cells was purified using Protein G GraviTrap (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... epithelial cells were treated 16-18 h prior to infection in growth media containing 200 µM 2,2’-dipyridyl (DPI, Sigma-Aldrich), 200 µM DPI and 200 µM ammonium iron (III ...
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bioRxiv - Microbiology 2020Quote: ... 150 mM NaCl and 0.1% Tween 20) were performed before incubating the membrane with the anti-FLAG antibody 1:5000 for 1 h (Sigma). Next ...
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bioRxiv - Neuroscience 2021Quote: ... next permeabilized for 20 minutes in PBST (PBS with 0.3% Triton X-100; BioShop) and blocked for 2 h in 3% BSA (Sigma-Aldrich) in PBST at RT ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were blocked with 5% skimmed milk in Tris Buffered saline-Tween for 1 h before incubation with primary antibody anti-myc tag monoclonal clone 4A6 (Millipore) diluted to final concentration of 200 ng mL−1 in 3% skimmed milk in TBST for 1h at room temperature ...
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bioRxiv - Physiology 2021Quote: ... was incubated for 2-3 h at 4 °C with 30 µl of FLAG-conjugated beads (anti-DYDDDDDK beads, Sigma) or empty beads (ThermoScientific) ...
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bioRxiv - Neuroscience 2021Quote: ... in either 4-well plates or P35 dishes (ThermoScientific) precoated for 4 h with 50 μg/ml poly-D-lysine (Sigma). Neurons were plated in DMEM containing 10 unit/mL penicillin ...