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Citations for Millipore Sigma :
1351 - 1400 of 10000+ citations for AS 1517499 CAS 919486 40 1 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Dried samples were suspended in 40 µL of a 40 mg/mL O-methoxylamine hydrochloride (MOX) (MP Bio #155405) in dry pyridine (EMD Millipore #PX2012-7) and incubated for 1 hour at 37 °C in a sand bath ...
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bioRxiv - Physiology 2022Quote: ... Dried samples were suspended in 40 μL of a 40 mg/mL O-methoxylamine hydrochloride (MOX) (MP Bio #155405) in dry pyridine (EMD Millipore #PX2012-7) and incubated for one hour at 37 °C in a sand bath ...
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bioRxiv - Cell Biology 2024Quote: ... Dried samples were suspended in 40 µL of a 40 mg/mL O- methoxylamine hydrochloride (MOX) (MP Bio #155405) in dry pyridine (EMD Millipore #PX2012-7) and incubated for one hour at 37 °C in a sand bath ...
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bioRxiv - Biochemistry 2024Quote: ... Dried samples were suspended in 40 µL of a 40 mg/mL O-methoxylamine hydrochloride (MOX) (MP Bio #155405) in dry pyridine (EMD Millipore #PX2012-7) and incubated for one hour at 37 °C in a sand bath ...
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bioRxiv - Genomics 2021Quote: ... The beads were washed two times with high-salt wash (50 mM Tris-HCl, pH 7.4, 1 M NaCl, 1 mM EDTA, 1% Igepal CA-630 (Sigma I8896), 0.1% SDS ...
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bioRxiv - Molecular Biology 2021Quote: ... and subsequently resuspended in PBS containing 1 % IGEPAL CA-630 (Sigma-Aldrich) and protease inhibitors ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Caspofungin stocks of 1 mg/ml (Sigma-Aldrich CAS#179463-17-3) were made from powder and suspended into ddH20 ...
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bioRxiv - Neuroscience 2022Quote: ... and mouse anti-GAD67 (1:10,000, MAB5406, EMD Millipore Corp, Temecula, CA) (Table 1) ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM DTT supplemented with 0.2 % (v/v) Igepal CA-630 (Sigma), 1 x cOmplete protease inhibitor tablet (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... 1% octyl-phenoxy-polyethoxyethanol (IGEPAL CA-630, Sigma-Aldrich, Cat. no. I3021) and HALT proteases and phosphatases inhibitor (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: Trypan blue solution 0.4% (Sigma, Cat. No. 93595, CAS: 72-57-1)
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bioRxiv - Neuroscience 2024Quote: ... and mouse anti-TH (1:10,000, MAB318, EMD Millipore Corp, Temecula, CA). Sections adjacent to tracer-labeled compartments through the basal forebrain were immunoreacted for CRF (BMA Biomedicals ...
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bioRxiv - Neuroscience 2023Quote: ... neuronal nuclei (NeuN) (1:100 dilution; Millipore, Temecula, CA, USA; Cat. # MAB377) and laforin (3µg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were permeabilized with 1% Triton X-100 (Sigma-Aldrich, CA, USA) in PBS for 3 hours at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were permeabilized with 1% Triton X-100 (Sigma-Aldrich, CA, USA) in PBS for 3 hours at room temperature ...
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bioRxiv - Immunology 2024Quote: ... 1-5 mM of CaCl2·2H2O (CAS 10035-04-8, Sigma-Aldrich) or 4 mM EGTA (CAS 67-42-5 ...
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bioRxiv - Biochemistry 2024Quote: ... and dissolved in trifluoroacetic acid (TFA) (Sigma Aldrich; Cas: 76-05-1) to a concentration of 1 wt% ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and heat-inactivated fetal calf serum (10%, Sigma, CAS 1943609-65-1). Media was partially exchanged on day 3 (800 μL ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.42 µM CoCl2·6H2O (Merck/Sigma-Aldrich, CAS number : 7791-13-1), 1.62 µM H3BO3 (Merck/Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.5 mM L-lysine (Merck/Sigma-Aldrich, CAS number : 56-87-1), 0.2 mM L-methionine (Merck/Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.2 mM L-histidine (Merck/Sigma-Aldrich, CAS number : 71-00-1), 0.4 mM L-isoleucine (Merck/Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... they were treated with 100μM TMZ (Sigma Aldrich, CAS #: 85622-93-1) over three weeks ...
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bioRxiv - Neuroscience 2024Quote: ... Odorants were liquid diluted 1:1 vol/vol in mineral oil (CAS 8042-47-5; Sigma-Aldrich) in amber vials ...
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bioRxiv - Microbiology 2020Quote: ... The affinity gel was then pelleted and washed twice using TEN buffer with 0.1% NP-40 and resuspended in equal volumes of NuPage LDS Sample Buffer (Thermo) containing 143 mM 2-Mercaptoethanol (Sigma-Aldrich). Western blotting using the elutes from affinity purification and the pre-purified input lysates were performed as described above with the following primary antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... The mixture was transferred to a pre-chilled tube containing 1 mL 100% ethanol (Commercial Alcohols P006EAAN) and 40 µL of 3M sodium acetate pH 5.3 (Sigma-Aldrich S7899). Samples were left at -20°C overnight and then centrifuged for 20 minutes at 13,000 x g at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... 150 mM KCl, 1% NP-40 [Sigma I8896-50ML], 0.5 mM DTT [Sigma, 646563], 100 µg/ml cycloheximide [Sigma C4859-1ML]). Conjugated beads were then resuspended in low-salt buffer and mixed with 50 µg each of anti-GFP antibodies Htz-GFP-19C8 and Htz-GFP-19F7 (Memorial Sloan-Kettering Monoclonal Antibody Facility ...
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bioRxiv - Biochemistry 2020Quote: ... Heparin (20 μl of 50 mg.ml−1) was reacted with 20 μl hydroxylamine-biotin in 40 μl 300 mM aniline (Sigma-Aldrich, UK) and 40 μl 200 mM acetate pH 6 for 48 h at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... nucleotides and ATP regeneration components were omitted and residual NTPs were depleted by the addition of 20 mM glucose and 40 units mL−1 hexokinase (Sigma H4502). In inhibitor controls ...
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bioRxiv - Cell Biology 2021Quote: Cells treated with SPS either alone or in conjunction with bafilomycin-A1 were collected and subsequently lysed in lysis buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 1% NP-40) supplemented with PhosStop phosphatase inhibitor cocktail (Sigma Aldrich) and protease inhibitor cocktail (Sigma Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... and mutants (lpg1-/- lpg5A-/lpg5B-) were lysed in 1% NP-40 in the presence of an protease inhibitor cocktail (Sigma, 11836170001). Equal cell equivalents were separated on a 10% SDS PAGE (Biorad ...
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bioRxiv - Cell Biology 2020Quote: ... Embryonic tissues were ground to a fine powder using a mortar and pestle on dry ice and lysed in RIPA buffer (150M NaCl, 50mM Tris pH8, 5mM EDTA, 1% NP-40 (Sigma, I8896), 0.5% sodium deoxycholate ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were treated for 2 h with 100 μM PALA and then lysed in NP40 Lysis Buffer (10 mM HEPES ph 7.4, 142 mM KCL, 5 mM MgCl2, 1 mM EGTA, 0.2% NP-40, and 1X Sigma protease inhibitor cocktail). Lysates were precleared with protein G-sepharose 4B beads (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... Cells were incubated in secondary antibody for 1 hour and then mounted on another glass coverslip using Mowiol 40-88 (Sigma-Aldrich). For immunostaining ...
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bioRxiv - Molecular Biology 2022Quote: ... Transfected cells were lysed for 20 minutes in pre-chilled Co-Immunoprecipitation buffer (50mM Tris-HCl pH 7.4, 150mM NaCl, 0.53% NP-40) supplemented with 1×protease inhibitor (Roche-Sigma, Cat no. # 11873580001) and 1×phosphatase inhibitor (Thermo Scientific™ ...
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bioRxiv - Cell Biology 2022Quote: ... samples were incubated in the dark for 30min with SDS buffer (100 mM Tris–HCl pH 8, 1% SDS, 250 mM NaCl, 5 mM EDTA) with 40 mM iodoacetamide (Sigma, #I1149). The resin was then resuspended in SDS buffer ...
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bioRxiv - Microbiology 2021Quote: ... 1% NP-40) in presence of Protease cocktail inhibitor (cOmplete™, EDTA-free Protease Inhibitor Cocktail, Sigma Aldrich, catalogue number 11873580001). Protein lysates were left rotating on-wheel at 4 °C for 15 min and cleared by 15 min of centrifugation at 4°C at 13200 (max speed) ...
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bioRxiv - Immunology 2020Quote: ... The baseline of the calcium signal was measured for approximatively 30 seconds before adding the soluble CD3/CD28 antibodies (1:40) (10971, StemCell) or 100ng/ml Ionomycin (I0634, Sigma-Aldrich) to measure the activation-induced calcium flux ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then lysed under denaturing conditions using the lysis buffer (40 mM HEPES KOH, pH 7.4, 1% SDS) containing 5 mM N-ethylmaleimide (NEM, Sigma-Aldrich, E1275) to prevent deubiquitination and boiled for 5 min ...
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bioRxiv - Genetics 2020Quote: Total protein extracted in a lysis buffer (50mM HEPES, 150mM NaCl, 10% glycerol, 1% Nonidet P-40, and 1mM EDTA) containing protease/phosphatase inhibitors (Sigma-Aldrich) was separated by SDS-PAGE and transferred onto Hybond-ECL membrane (Amersham ...
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bioRxiv - Microbiology 2020Quote: ... All Strain were maintained in 40 % glycerol, 60 % Lysogeny Broth (LB, 1 /L, 15 g Agar, 10 g Tryptone (Sigma-Aldrich), 5 g Yeast Extract(Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2023Quote: ... followed by an incubation at 40°C for 1 h and then adding 70 μl of MSTFA (N methyl-N-trimethylsilyltrifluoroacetamide; Sigma-Aldrich) followed by another incubation at 40°C for 2 h ...
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bioRxiv - Genomics 2022Quote: ... Tagmentation reaction was performed at 55°C for 30 minutes and stopped by adding 11 μL 2X Stop buffer (40 mM EDTA, 1 mM Spermidine (Sigma, S0266)) to each well ...
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bioRxiv - Immunology 2023Quote: ... The immobilized liposomes were completely removed with an injection of 40 mM 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS) (Sigma-Aldrich) (20 μl ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were pipetted up and down 50 times until complete disaggregation and neutralized by the addition of 40 μL of 1% cell culture grade BSA (Sigma # A8806) to avoid the formation of clumps after dissociation ...
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bioRxiv - Cell Biology 2022Quote: ... pulverized in liquid nitrogen and protein was extracted using a 1x radioimmunoprecipitation assay buffer (50mM Tris-HCl pH 8.0, 150 mM NaCl, 1% Nonidet®40 Substitute (74385; Sigma-Aldrich), 0.5% sodium deoxycholate ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting circle DNA can be amplified (1 h 40 min amplification at 37 °C, Duolink In Situ Detection Reagents Green, Sigma-Aldrich) and visualized by fluorescently labeled complementary oligonucleotide probes incorporation ...
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bioRxiv - Plant Biology 2023Quote: ... Transfection of protoplasts with the plasmid DNA construct(s) of interest was performed by adding 1 volume PEG-Ca2+ solution (40% [w/v] PEG 4000 (Sigma Aldrich), 0.2 M D-mannitol ...
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bioRxiv - Molecular Biology 2023Quote: ... Gold fiducials (6 nm; BSA tracer; Aurion, Wageningen, Netherlands) were buffer exchanged into 40 g L-1 sea salts (Sigma Aldrich) using centrifugal ultrafiltration unit (100 kDa MWCO ...
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bioRxiv - Cell Biology 2024Quote: ... Knockdown efficiency and p130Cas over-expression levels were confirmed by Western blotting of cell lysates in radioimmunoprecipitation assay (RIPA) buffer (25 mM Tris-HCl, pH 7.5 [Sigma-Aldrich], 150 mM NaCl [JT Baker], 1% NP-40 [Sigma-Aldrich] ...
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A non-invasive method to sample immune cells in the lower female genital tract using menstrual discsbioRxiv - Immunology 2024Quote: ... the cell pellet was resuspended in 1 mL freezing medium (50% FBS, 40% RPMI with L-glutamine, 10% DMSO (Millipore Sigma)) ...