Labshake search
Citations for Millipore Sigma :
1301 - 1350 of 10000+ citations for AS 1517499 CAS 919486 40 1 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... then scraped into 3 ml ice cold lysis buffer (10 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% NP-40 substitute (Sigma 74385), 1x Protease inhibitor (Roche ...
-
bioRxiv - Bioengineering 2022Quote: ... The ethanol was removed and the cells were covered with 1 mL of 40 mM Alizarin Red solution (Sigma-Aldrich) for 10 minutes ...
-
bioRxiv - Developmental Biology 2021Quote: ... cells from two YapF/F;TazF/F control brains or three YapF/F;TazF/F;Emx-Cre dKO brains were resuspended in 1 ml of sorting buffer (PBS, 0.5% BSA, RNAsin 1:20) and filtered through a 40-μm Flowmi cell strainer (Sigma #136800040). Cells were sorted on a FACSAria cell sorter (BD Biosciences ...
-
A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... washed twice in 1x PBS and resuspended in 2 ml co-IP lysis buffer (50 mM Tris-HCl pH 7.5, 1% Nonidet P 40 Substitute (Sigma #74385), 0.25% deoxycholate Na ...
-
bioRxiv - Cell Biology 2020Quote: ... 1 mM dithiothreitol (DTT) and 0.1% (vol/vol) Nonidet P-40) supplemented with a cocktail of protease inhibitors (Sigma-Aldrich). Lysates were cleared by centrifugation at 16,000 x g for 20 min at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... The pellet of 100 mL was resuspended in 1 mL cold fractionation buffer (40 mM HEPES, 0.1 mM EDTA, 1x cOmplete™ Protease Inhibitor Cocktail (Roche Sigma)) and the pellet of 1 mL lysed with Bug Buster (pellet sample ...
-
bioRxiv - Immunology 2020Quote: ... lysed with lysis buffer (50 mM Tris-HCl [pH 7.4], 150 mM NaCl, 10% glycerol, 1% NP-40, protease inhibitor cocktail (Sigma Aldrich) and phosphatase inhibitor (Roche) ...
-
bioRxiv - Microbiology 2021Quote: ... DOPS or BMP were diluted from 5 mg/ml stock solutions in ethanol to a final concentration of 10 or 40 μg/ml in PBS++ supplemented with 1 mg/ml BSA (Sigma) and immediately added to cells ...
-
bioRxiv - Synthetic Biology 2021Quote: ... and the cells were resuspended in 40 ml of transformation medium at pH 5.8 (1× MS plant salt mixture without NH4NO3 (Sigma-Aldrich), 1% w/v sucrose ...
-
bioRxiv - Cell Biology 2020Quote: Protein lysates were prepared using RIPA lysis buffer (50mM Tris pH 7.4, 150mM NaCl, 5mM EDTA, 1% NP-40) containing protease and phosphatase inhibitors (Sigma Aldrich). The protein lysates were separated on 8-12% polyacrylamide gels and transferred to nitrocellulose or PVDF membranes ...
-
bioRxiv - Plant Biology 2023Quote: Frozen leaf tissue was ground in two volumes of protein extraction buffer (150 mM NaCl, 50 mM Tris [pH 7.5], 0.1% Nonidet P-40 [Sigma-Aldrich], 1% plant protease inhibitor cocktail [Sigma-Aldrich] ...
-
bioRxiv - Cancer Biology 2023Quote: ... fixed cells were pelleted and washed twice with PBS and resuspended in 300 μL hypotonic buffer (1 g/L sodium citrate buffer, 0.1% Triton-X 100) supplemented with 40 μg/mL propidium iodide (Sigma-Aldrich) and 100 μg/mL of RNase A (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2023Quote: ... were dissolved in HEPES-aCSF in the presence of non-NMDA glutamate receptor blockers (CNQX, Tocris, 0190, 40 µM; Nimodipine, Tocris, 0600, 1 µM; LY-367385, Sigma, L4420 ...
-
bioRxiv - Immunology 2023Quote: ... Cells were then lysed in RIPA-buffer (50 mM Tris-HCl pH 8.0, 1% w/v NP-40 (Sigma, 492018), 0.5% w/v sodium deoxycholate (Sigma ...
-
bioRxiv - Cell Biology 2023Quote: For LC-MS/MS, proteins were reduced and alkylated in SDC buffer (1% Sodium deoxycholate, 40 mM 2-Cloroacetamide (Sigma), 10 mM TCEP (Thermo ...
-
bioRxiv - Biochemistry 2024Quote: Cells were harvested and resuspended in lysis buffer (40 mM HEPES pH 7.5, 10 mM NaCl, 1% Triton X-100, 1X protease inhibitor cocktail (Sigma P2714), 20 mM β-glycerophosphate ...
-
bioRxiv - Biochemistry 2024Quote: ... The purified protein sample was concentrated to 40 mg mL-1 using Amicon Ultra-15 3K MWCO Centrifugal Filter Unit (Merck Millipore) and subsequently diluted 1:4 with MilliQ water (yielding 10 mg mL-1 of protein in 1x crystal buffer (10mM Tris ...
-
bioRxiv - Cell Biology 2024Quote: ... HEK293T were plated at 40% confluency on 60 mm dishes and transfected with the gene specific shRNA pLKO.1 plasmid (Sigma), packaging plasmid (Δ8.9 ...
-
bioRxiv - Neuroscience 2024Quote: ... in 0.15 M KCl TRAP wash buffer (10 mM HEPES-KOH at pH 7.4, 5 mM MgCl2, 150 mM KCl, 1% NP-40, supplemented with 100 µg/mL cycloheximide [Millipore Sigma C7698-1g] in methanol ...
-
bioRxiv - Neuroscience 2024Quote: ... homogenized in cytosol lysis buffer (10 mM Hepes, 40 mM KCl, 3 mM MgCl2, 5% glycerol, 0.2% NP40, 1 mM DTT, cOmplete protease inhibitors, Sigma-Aldrich) and incubated for 30 min at 4°C with rotation ...
-
bioRxiv - Biochemistry 2023Quote: ... 1% Nonidet P-40) in the presence of a protease inhibitor cocktail and phenylmethanesulfonyl fluoride (Sigma-Aldrich, St. Louis, MO).
-
bioRxiv - Bioengineering 2023Quote: Medium in the apical perfusion channel was replaced by medium containing 1 mg/ml FITC-dextran (40 kDa, Sigma, FD40). To evaluate barrier integrity imaging was performed at different time-points ...
-
bioRxiv - Cell Biology 2022Quote: ... Thawed pellets were resuspended with 200-300 µL native lysate buffer (1% NP-40 with 1X cOmplete protease inhibitor cocktail [SKU, 11836145001, Roche], 0.5 mM PMSF, and 1% digitonin [SKU D141, Millipore Sigma]) and 200 µL 0.5 mm zirconia/silica beads (cat ...
-
bioRxiv - Genomics 2022Quote: MB135-iDUX4 myoblasts were treated with or without doxycycline for 14 h and then trypsinized prior to lysis on ice in 1 mL of Lysis Buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% NP-40) containing protease inhibitors (Sigma Aldrich). Lysates were precleared using Protein G Sepharose (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2022Quote: ... 250 μg of total protein samples were made up to 500 μl total volume with 1% NP-40 buffer and incubated overnight at 4°C with 1 μl of mouse anti-Flag primary antibody (#F1804; Sigma). Subsequently ...
-
bioRxiv - Microbiology 2022Quote: ... and then lysed by incubating at 4 °C with IP lysis buffer (20 mM NEM, 1% NP-40 alternative (Millipore) in IP buffer base (50mM Tris-HCl (pH 7.4) ...
-
bioRxiv - Plant Biology 2022Quote: ... and re-suspended to a concentration of 40 µg Chl mL−1 in fresh medium supplemented with 10% ficoll (Sigma) and 2 mM NaHCO3 (Sigma) ...
-
bioRxiv - Neuroscience 2022Quote: For differentiation of OPC to OL: OPC medium was supplemented with 40 ng ml−1 3,3ʹ,5-Triiodo-L-thyronine (T3) (Sigma; T6397)52 ...
-
bioRxiv - Microbiology 2023Quote: ... Cleared cell lysates were immunoprecipitated using 1 μg FLAG-M2 antibody coupled to 40 μL of packed protein G Sepharose beads (Cat GE17-0618-01, Sigma) for 3 h at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: ... 25 mM HEPES-NaOH [pH. 7.9], 1 mM MgCl2, 0.2 mM EDTA, 0.5% NP-40, 600U/mL Benzonase [70664, Millipore], 1x protease inhibitor) and incubated for 4 hours at 4°C with rotation ...
-
bioRxiv - Cell Biology 2023Quote: ... and 0.3% NP-40) containing 10 µg/ml LPC (leupeptin, Pepstatin A, and chymostatin) and 1% phosphatase inhibitor cocktail 2 (P5726, SIGMA), followed by clarification of the lysate by centrifugation at 15,000 rpm (21,000 g ...
-
bioRxiv - Biophysics 2022Quote: ... Samples were embedded in GLOX based imaging buffer[43] as oxygen scavenging system (40 mg/mL-1 catalase (Sigma-Aldrich), 0.5 mg/mL-1 glucose oxidase ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were harvested with 800µl NP-40 buffer (20mM Tris pH=8, 150-200mM NaCl, 0.5mM EDTA, 1% NP-40, 1mM DTT, 5% glycerol and protease inhibitor cocktail; Sigma-Aldrich). Protease inhibitor cocktail (Bimake ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were harvested with 300μl extraction buffer (50mM Tris pH=8, 150mM NaCl, 0.5mM EDTA, 1% NP-40, 5%, 1mM DTT, and protease inhibitor cocktail [Sigma-Aldrich]). Cell extracts were sonicated and centrifuged at 4°C for 15 min at 16,000 ×g ...
-
bioRxiv - Bioengineering 2023Quote: ... and fibrin support materials were prepared with FITC-labeled diffusants (either 1 µg/mL diazide-PEG-FITC, 10 µg/mL of 10, 20, 40, 60, 150, 250 kDa FITC-dextran, Sigma). 150 µL of the diffusant-laden support materials were loaded into a clear bottom ...
-
bioRxiv - Cell Biology 2024Quote: ... two million cells were washed in 1 ml PBS and lysed in 500 µl ice-cold REAP buffer (0.1% NP-40 in 1x PBS) supplemented with 1 mM PMSF (Sigma-Aldrich), 1X cOmplete Protease Inhibitor Cocktail (Roche ...
-
bioRxiv - Immunology 2024Quote: ... The cells were washed three times in PBS 1× and incubated for 1 hour at 37°C with 40 μL of mouse anti-DENV complex monoclonal antibody MAB8705 (Merck Millipore) diluted 1:200 in PBS 1× ...
-
bioRxiv - Biochemistry 2024Quote: ... washed one time with 40 ml cold 1 M sorbitol and resuspended in a Ficoll buffer (18% Ficoll type 400 (Sigma), 20 mM KH2PO4 pH 6.8 ...
-
bioRxiv - Immunology 2024Quote: ... Tissues were homogenized using an electric homogenizer in lysis buffer (1% NP-40, 100 mM Tris, pH 8.0, and 150 mM NaCl) containing a protease inhibitor mixture (Sigma Aldrich) and shaken on ice for 30 mins ...
-
bioRxiv - Biochemistry 2024Quote: ... Cells were lysed in lysis buffer (150mM NaCl, 50mM HEPES pH 7.5, 1% NP-40 substitute) supplemented with Roche cOmplete Protease Inhibitor Cocktail (Sigma, 11836145001), PhosSTOP™ Phosphatase Inhibitor Cocktail (Roche ...
-
bioRxiv - Neuroscience 2021Quote: ... cells were subcultured onto glass coverslips (hand-cut to ∼5 x 5 mm) coated with 0.1 mg/mL Poly-D-Lysine (Millipore-Sigma, #27964-99-4) in 35-mm cell culture dishes (Corning ...
-
bioRxiv - Biochemistry 2020Quote: ... methanol (LC-MS grade), 2,5-dihydroxyacetophenone (DHA, ≥99.5%, Ultra pure), and anhydrous sodium acetate (NaOAc, ≥99%) were all purchased from Sigma Aldrich (Zwijndrecht, The Netherlands) and used without further purification.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Curcumin (>90% pure) was purchased from Cayman (Ann Arbor, MI) and piperine (>99% pure) was purchased from Sigma Aldrich (St. Louis, MO). Stock solutions of curcumin and piperine were prepared in dimethyl sulfoxide (DMSO) ...
-
bioRxiv - Cell Biology 2021Quote: ... and immersed into the oil phase (~125 mL) consisting of hexanes (99%) and 3% (v/v) Span 80 (Fluka, 85548 or Sigma-Aldrich, S6760). 10 mL of gel mixture was extruded through SPG membranes under nitrogen pressure of approximately 7 kPa ...
-
bioRxiv - Biochemistry 2020Quote: ... followed by the carbon source (glucose crystals or 99 % glycerol) and filtered sterile using a 0.22 μm pore-size Millex®GP PES filter (Merck-Millipore, Carrigtwohill, Ireland). For the cycA tests ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... BDE-99 was dissolved in corn oil and filtered using a 0.22 μm Millipore Express Plus Membrane filter (EMD Millipore, Temecula, California). All mice used in this study were housed according to the Association for Assessment and Accreditation of Laboratory Animal Care International guidelines ...
-
bioRxiv - Biophysics 2022Quote: ... low gelling temperature agarose (Catalog number: A0701) and poly(vinyl alcohol) (MW 146,000 – 186,000 99+% hydrolyzed) (PVA) from Sigma-Aldrich (St. Louis, MO). We purchased chloroform (ACS grade ...
-
bioRxiv - Cell Biology 2022Quote: ... mounted on an internal pressure micro kit extruder (SPG Technology Co.) and immersed into an oil phase (∼125 mL) consisting of hexanes (99%) and 3% (v/v) Span 80 (Sigma-Aldrich, S6760). 10 mL of gel mixture was extruded into the oil phase under nitrogen pressure of approximately 7 kPa ...
-
bioRxiv - Neuroscience 2024Quote: ... cells were subcultured on glass coverslips (hand-cut to ∼5 x 5 mm) coated with 0.1 mg/mL Poly-D-Lysine (Millipore-Sigma, #27964-99-4) in 35-mm cell culture dishes (Corning ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... bisphenol C-Cl (CAS no. 14868-03-2) and bisphenol S (CAS no. 80-09-1) were bought from Sigma-Aldrich. Compounds were dissolved in dimethyl sulfoxide (DMSO ...