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Citations for Millipore Sigma :
1301 - 1350 of 4708 citations for Chocolate Agar with Bacitracin for Haemophilus isolation 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... media in each well was replaced with 100µL (for 96-well plate format) or 200µL (for 48-well plate format) complete DMEM in addition to 2µM ionomycin (Sigma-Aldrich) and 5mM CaCl2 with or without 10µM furimazine (unless indicated otherwise) ...
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bioRxiv - Neuroscience 2022Quote: ... and seeded at a density of 2 × 106 cells per well (6-well plates) or 0.5 × 106 cells per well (24-well plates) into PDL/Laminin (Sigma)-coated plates with neuronal medium plus 0.5 μM RA ...
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bioRxiv - Cell Biology 2023Quote: ... Infected CAD5 cells were seeded in ELISPOT plates (multiscreen 96-well filter plates with high protein-binding Immobilon-P membrane, 0.45 µm, Millipore) activated with 70% ethanol ...
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bioRxiv - Microbiology 2023Quote: ... Dilutions were plated on TY plate and in TY plate containing either 750 µM of DEA NONOate (Sigma-Aldrich), 0.1% of NaClO (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... regular NGM plates served as a control and treatment plates were supplemented with 400μM Coenzyme A (Sigma-Aldrich, C4780).
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bioRxiv - Immunology 2024Quote: ... and 1 × 106 cells were transferred per well to 96-well filter plate (#MSDVN6550 MultiScreen DV Filter Plate, Millipore). After adhering yeast cells ...
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bioRxiv - Microbiology 2022Quote: ... pseudolongum strain UMB-MBP-01 was isolated from the feces of C57BL/6J mice through passages and screening on Bifidus Selective Medium (BSM) agar (Sigma-Aldrich, St. Louis, MO, USA), as previously described [34] ...
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bioRxiv - Microbiology 2022Quote: ... or on 7H11 agar supplemented with ADS or OADC (ADS plus 0.05% oleic acid and 0.004% catalase (Sigma Aldrich, cat. No. C9322, St Louis, MO) or ADC (ADS plus 0.004% catalase ...
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bioRxiv - Microbiology 2023Quote: Four fungal plugs obtained from monosporic cultures of each of the 113 Trichoderma isolates were placed on Potato Dextrose Agar (PDA) (Sigma-Aldrich, St. Louis, MO, USA) medium covered by a cellophane layer and incubated at 26 °C for 3 days in the dark ...
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bioRxiv - Microbiology 2020Quote: ... Total mRNA was extracted from 0.5 x 106 cells using a single-step RNA isolation reagent- TRI reagent (T9424, Sigma-Aldrich) at specified time points ...
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bioRxiv - Biophysics 2021Quote: ... Dead cells were removed six days after T-cell isolation with a density-dependent gradient centrifugation step (Histopaque 1119, Sigma). Antigen-experienced T-cells were used for experiments on day 7 – 9.
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bioRxiv - Genetics 2020Quote: ... Enrichment of the chloroplast from the leaf samples was performed using ± 2 cm2 of leaf tissue with the Chloroplast Isolation Kit (Sigma, CPISO-1KT After this the DNA was extracted using a modified C-Tab protocol (Doyle JJ ...
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bioRxiv - Developmental Biology 2021Quote: ... L3 larvae or pupae of different stages) or tissues (adult head, thorax or abdomen or IFMs) were collected for RNA isolation using the TRI reagent (Sigma) based protocol ...
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bioRxiv - Biophysics 2020Quote: ... Dead cells were removed on day 6 after T-cell isolation by means of density-dependent gradient centrifugation (Histopaque 1119, Sigma). Antigen-experienced T-cells were used for experiments on day 7 – 9.
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bioRxiv - Cell Biology 2021Quote: ... after which we incubated a 20µL pre-isolation aliquot of the filtrate with 0.1µg/mL 4′,6-diamidino-2-phenylindole (DAPI) (Millipore-Sigma, cat. #D9542) and mounted on a slide for imaging (Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... after which we incubated a 20µL pre-isolation aliquot of the filtrate with 0.1µg/mL 4′,6-diamidino-2-phenylindole (DAPI) (Millipore-Sigma, cat. #D9542) and mounted on a slide for imaging (Fisher ...
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bioRxiv - Plant Biology 2020Quote: ... Arabidopsis root nuclei were isolated by chopping the roots in 1 ml of Nuclear Isolation Buffer (CELLYTPN1-1KT, Sigma-Aldrich) and incubated for 15 minutes on a rocking shaker at 4°C ...
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bioRxiv - Systems Biology 2020Quote: Aliquots of 100,000 CD14+CD16− monocytes were thawed on ice for 5 minutes then immediately resuspended in 20μL of EZ Nuclei Isolation Buffer (Sigma-Aldrich), and incubated for 5 minutes on ice ...
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bioRxiv - Genomics 2020Quote: ... nuclei were isolated by gently homogenizing tissue with a pestle in cold Nuclei Isolation Buffer from a Nuclei PURE Prep Kit (Sigma). Nuclei were pelleted and supernatant removed before being digested by incubating in 70 µl PBS ...
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bioRxiv - Cell Biology 2020Quote: ... TSA (0.6 mg/kg/day for 15 days to obtain FAPs for co-colture and for EVs isolation or 21 days for the transplant experiments; #T8552, Sigma), dissolved in DMSO solution or in DMSO alone as vehicle control (CTR) ...
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bioRxiv - Plant Biology 2020Quote: Total RNA for sRNA-seq and RNA-seq libraries was isolated using the TRI Reagent RNA isolation reagent (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... coli cells that contained pL4440-CP were grown in LB supplemented with carbenicillin (100 μg/mL) followed by plasmid DNA extraction with the High Pure Plasmid Isolation Kit (Sigma). For the in vitro synthesis ...
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bioRxiv - Plant Biology 2024Quote: ... The insoluble pellet was washed five times repeatedly by resuspension in the isolation buffer containing 0.1 g of quartz sand (Sigma-Aldrich) and vortex ...
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bioRxiv - Cell Biology 2023Quote: ... treated with LNPs containing mRNA sequences of either the anti-NSP9 nanobody or tdTomato were used for total mRNA isolation by TRI Reagent (Millipore-Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended in isotonic mitochondrial isolation buffer (250 mM sucrose, 1 mM EDTA, 20 mM Tris-HCl, pH 7.4, protease inhibitor cocktail SigmaFAST; Sigma; S8820). To isolate mitochondria ...
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bioRxiv - Cell Biology 2023Quote: ... were allowed for growth in 6-cm cell culture plates for 48 h and subjected to isolation of the cytoplasmic and nuclear fractions by using a relevant separation kit (N-3408-200ML, Sigma). During this procedure ...
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The Single-Cell Transcriptome Program of Nodule Development Cellular Lineages in Medicago truncatulabioRxiv - Plant Biology 2023Quote: ... Root segments were moved to a glass plate with 200 μL of modified Nuclei Isolation Buffer (NIB; Conde et al., 2021) containing 0.5 U/mL Protector RNase Inhibitor (Sigma Aldrich). After samples were fragmented with a sterile razor blade ...
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bioRxiv - Cell Biology 2023Quote: ... histone H1.2 knockout heterozygous (Het) and homozygous (KO) mice are isolated using the Nuclei Isolation ‘Kit Nuclei EZ Prep’ (NUC101, Sigma). Then the nuclei are treated with 500 µM MNNG with fresh-made NAD+ (N0632 ...
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bioRxiv - Cell Biology 2023Quote: ... Monocyte-depleted PBMCs or isolated CD4+ T cells were stimulated immediately after their respective isolation with PHA (1 µg/mL, Sigma) or Dynabeads® Human T-Activator CD3/CD28 (Thermofisher) ...
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bioRxiv - Genomics 2023Quote: ... Frozen cells were thawed and added to a Dounce homogenizer containing 1 mL of nuclei isolation buffer (NIB: 10 mM TrisHCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% Igepal (Millipore Sigma #18896), and 1 x protease inhibitors (Millipore Sigma #118735800001)) ...
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bioRxiv - Cell Biology 2023Quote: ... livers were perfused with HBSS containing collagenase and pronase via the portal vein prior to mechanical disruption of tissue and isolation of HSCs via an Optiprep density gradient (Sigma). Cells were cultured in high glucose DMEM enriched with L-glutamine ...
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bioRxiv - Immunology 2023Quote: ... The cells were then centrifuged at 1200 rpm at 4°C and RNA isolation was performed with Tri-reagent (Sigma) following the manufacture’s protocol ...
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bioRxiv - Immunology 2023Quote: ... EasySep™ Human NK Cell Isolation Kit) and were frozen down in CS10 CryoStor cell cryopreservation media media (Sigma-Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... The beads were washed once with 1 mL of isolation buffer and then incubated overnight at 4°C with 10 μg of either anti-CD9 (clone CBL162, Millipore), anti-L1CAM (clone 5G3 ...
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bioRxiv - Microbiology 2023Quote: ... Bacteriophage DNA was isolated using the Phage DNA Isolation Kit (Norgen) and plasmids were isolated with the GenEluteTM Plasmid Miniprep Kit (Sigma). DNA purification from agarose gel or enzymatic reactions was performed with the NucleoSpin Gel and PCR clean up kit (Macherey-Nagel) ...
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bioRxiv - Microbiology 2023Quote: ... and DNA was extracted from a 500 mg crushed root sample using GenEluteTM Soil DNA Isolation kit (Sigma-Aldrich, Canada) with slight modifications ...
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bioRxiv - Cell Biology 2024Quote: ... A portion of the LV and septum was minced in a beaker on ice for 3 minutes and subsequently was homogenized using a glass dounce homogenizer at 4°C for 3 minutes in 10 mL of isolation buffer (200 mM Mannitol [M9647, Sigma], 60 mM sucrose [S7903, Sigma], 5 mM KH2PO4 [P5379, Sigma] ...
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bioRxiv - Cell Biology 2024Quote: ... A portion of the LV and septum was minced in a beaker on ice for 3 minutes and subsequently was homogenized using a glass dounce homogenizer at 4°C for 3 minutes in 10 mL of isolation buffer (200 mM Mannitol [M9647, Sigma], 60 mM sucrose [S7903, Sigma] ...
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bioRxiv - Cell Biology 2024Quote: ... A portion of the LV and septum was minced in a beaker on ice for 3 minutes and subsequently was homogenized using a glass dounce homogenizer at 4°C for 3 minutes in 10 mL of isolation buffer (200 mM Mannitol [M9647, Sigma] ...
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bioRxiv - Cancer Biology 2024Quote: ... The isolation of nuclear proteins was achieved with the CelLytic™ NuCLEAR™ extraction kit (Sigma Aldrich, St. Louis, MO) using the manufacturer’s recommended protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei isolation was achieved using either ST-based buffers as done before (Slyper et al) or by EZ-lysis method (Sigma). Briefly ...
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bioRxiv - Genetics 2021Quote: Exponentially growing cells at OD600 nm = 0.2 were injected into the microfluidics chambers of a CellASIC plate (Y04C-02 plate for haploid cells, CellASIC ONIX2 Microfluidic System, Millipore), through the inlet wells ...
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bioRxiv - Plant Biology 2022Quote: Col-0 and rve 4 6 8 seedlings were germinated on 0.5x MS control plates and 0.5x MS plates supplemented with 50 μM MG132 (Millipore Sigma; 474787) and grown under 12:12 photoperiod conditions as described above for 6 days prior to imaging.
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bioRxiv - Microbiology 2021Quote: Cells obtained from overnight cultures grown in YPD broth at 37°C were spotted in serial 10-fold dilutions on YP plates containing 2% glycerol or YPD plates containing 0.05 mg/ml of Congo red (Millipore Sigma) or 0.01 mg/ml of calcofluor white (Millipore Sigma) ...
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bioRxiv - Immunology 2021Quote: Panning was performed against monomeric NTD directly absorbed to polystyrene plates (Maxisorb 96-well microtiter plates; Nunc, Sigma-Aldrich, USA). Blocking of plates and phages was conducted for 60 min using two blocking solutions ...
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bioRxiv - Synthetic Biology 2023Quote: HEK293T-LP cells sourced from Matreyek et al38 were plated into a 48 well plate according to the plate reader assay and treated with 0 to 2 ng/μl dox (Sigma) to induce BFP expression ...
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bioRxiv - Physiology 2023Quote: ... were transferred to OP50-seeded plates or RNAi-seeded NGM plates supplemented with 50 μM of 5-fluoro-2-deoxyuridine (FUDR, Sigma) to prevent progeny growth ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then recovered in 10ml of YES at 25°C overnight and subsequently inoculated on selective plates - YES plates containing 100µg/mL G418 sulphate (Sigma Aldrich), 50µg/mL hygromycin B (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... The plate was blocked with 5% BSA (Sigma) in PBS for 3 hours at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... TLC plates were sprayed with Primuline (SIGMA 206865) (1 mg/ml fresh stock in ultrapure water ...