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Citations for Millipore Sigma :
1201 - 1250 of 4708 citations for Chocolate Agar with Bacitracin for Haemophilus isolation 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... cells were subjected to nuclei isolation following 10x Genomics manufacturer’s protocol in the presence of RNAse inhibitor (Sigma) and DTT (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA isolation from spinal cord tissue and fibroblasts pellets was performed with TRIzol Reagent (Sigma Aldrich, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... EV-isolation media was supplemented with either sphingomyelinase inhibitor drugs or vehicle: 0.2% DMSO (Sigma-Aldrich, D8418-100ML), 10 µM GW4869 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... The pellets were then resuspended in nuclei isolation buffer containing 0.05% Empigen BB detergent (45165-50ML; Sigma-Aldrich) (nuclei isolation buffer+ ...
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bioRxiv - Plant Biology 2021Quote: ... basal salt mixture including MES buffer (Duchefa Biochemies; cat No. M0254.0050) supplemented with 1 % plant agar (pH 5.7) (Sigma, cat. no. 9002-18-0) and 1 % sucrose ...
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bioRxiv - Microbiology 2021Quote: ... Broth cultures were then streaked onto modified SCAI medium [42] which comprised of Simmons citrate agar (Oxoid, Hamphire, United Kingdom) supplemented with 1% myo-inositol (Sigma, St. Louis, USA) and amoxicillin (10 mg/L ...
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bioRxiv - Plant Biology 2021Quote: ... containing 1% (w/v) sucrose unless indicated otherwise and solidified with 1% (w/v) EDTA-washed Agar Type M (Sigma-Aldrich, Steinheim, Germany), as described (Schulten et al. ...
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bioRxiv - Microbiology 2022Quote: ... Durham, NC) or Brucella agar (Becton Dickinson, Canaan, CT) supplemented with 500uL of PBS with 0.1% taurocholic acid (Millipore-Sigma, St. Louis, MO) for C ...
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bioRxiv - Cell Biology 2021Quote: The oocytes were fixed overnight in 3% glutaraldehyde (AGR1012, Agar Scientific, Stansted, UK) in 0.1 M sodium cacodylate buffer (C0250, Sigma Aldrich, St. Louis, USA) (pH 7.2–7.8 ...
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bioRxiv - Microbiology 2020Quote: ... 2 mL of the upper phase was transferred to a new tube for plating onto Sabouraud Glucose Agar (SGA) containing 50 μg/mL Chloramphenicol (Sigma-Aldrich, Taufkirchen, Germany). Of this 2 mL ...
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bioRxiv - Molecular Biology 2021Quote: ... heat-shocked in water (60°C for 30 min) and plated directly on sorbose agar containing cyclosporin A (5 μg/ml; Sigma, cat. no. 30024). Genomic DNA was extracted as previously described (15) ...
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bioRxiv - Microbiology 2022Quote: ... Virus was removed and 2 mL of overlay media (EMEM 1X Lonza 12-684F, 5 % FBS HyClone, 0.6 % purified Agar OXOID #LP0028, 0.12% NaHCO3 Sigma #S5761, 0.01% Dextran Sigma #D9885) was added ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Branch bark and cambium were removed at canker margin and canker tissue was streaked onto nutrient agar (Sigma-Aldrich, St. Louis, MO, USA) plate ...
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bioRxiv - Microbiology 2023Quote: ... For media preparation agar, tryptone and yeast extract were purchased from HiMedia (Mumbai, India) and crab shell chitin from Sigma (cat no 417955) was used.
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... a single colony for each replicate was picked following overnight growth on LB agar and added to 5 ml of LB broth (Sigma-Aldrich, United Kingdom). LB was supplemented with 4 µg/ml cefotaxime for transconjugant culture ...
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bioRxiv - Microbiology 2024Quote: ... Membrane protein topology was assayed by plating the resulting reporter strains on dual-indicator plates containing LB agar supplemented with 80 μg/ml 5-bromo-4-chloro-3-indolyl phosphate disodium salt (X-Phos) ([Sigma Aldrich], RES1364C-A101X) and 100 μg/mL 6-chloro-3-indolyl-β-d-galactoside (Red-Gal ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The entire reaction was quickly removed from the assay plate and filtered on Multiscreen filter plate (Millipore) and then washed four times with ice-cold stop buffer ...
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bioRxiv - Microbiology 2022Quote: Culture plate lid: Sealing mat for 2 mL square deep well plates (Sigma-Aldrich, cat. no. AXYAM2MLSQ)
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bioRxiv - Evolutionary Biology 2023Quote: ... cultures were maintained in transparent 48-well plates covered with Breath-Easy breathable plate-seals (Sigma Aldrich). Ancestral populations were maintained under identical nutrient ...
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bioRxiv - Microbiology 2024Quote: ... and the supernatant was discarded by placing the plate on 96-well plate magnetic separator (Millipore Sigma). Next ...
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bioRxiv - Physiology 2024Quote: ... and loaded onto preparative TLC plates (Supelco TLC silica gel-60 glass plates; Millipore Sigma cat#1.00390.0001), and separated through 3 phases (as described by Choa et al58) ...
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bioRxiv - Microbiology 2021Quote: ... plates were processed by transferring 150 ul of eluate from each well to a 0.2um filtration plate (Millipore, Multiscreen HTS GV 0.22um Filter Plate MSGVS22 ...
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bioRxiv - Developmental Biology 2021Quote: ... the TLC plate was air-dried and lipids were visualized by spraying the plate with primuline (Sigma-Aldrich) solution (5% primuline in acetone:water 8:2 v/v) ...
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Exometabolite dynamics over stationary phase reveal strain-specific responses to nutrient limitationbioRxiv - Microbiology 2020Quote: ... we used sterile filter plates with 0.22-μm-pore polyvinylidene difluoride (PVDF) filter bottoms (MultiScreen GV Filter Plate, 0.22 μm, MSGVS2210, Millipore). Prior to use ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were split into 15-cm plates at 5,000 cells per plate in 50 µM ganciclovir (Sigma). Surviving colonies were isolated with cloning cylinders and expanded in 50 µM ganciclovir ...
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bioRxiv - Synthetic Biology 2022Quote: ... The plates were then replica plated onto SC-Arg plates with and without L-canavanine sulphate (Sigma-Aldrich) added to a final concentration of 60 µg ml-1 ...
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bioRxiv - Microbiology 2024Quote: ... biofilms were prepared using glass microscopy-compatible plates (Greiner high and medium binding 96 well plates, Sigma-Aldrich) according to the method described above ...
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bioRxiv - Cell Biology 2024Quote: ... and fibroblasts were seeded at a density of 1.6×105 cells per well in 6-well plates or 1.2×106 cells in p100 plates coated with 0.1% gelatin (SIGMA). Virus containing-medium was collected 48 hours after transfection (day 3) ...
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bioRxiv - Cell Biology 2022Quote: 70 µl of cells were immobilized on glass bottom wells of imaging plates (Mobitec, Imaging Plate 96 CG 1.5, Order #: 5242-20) coated with Concanavalin A (0.45 mg/ml, Sigma) and overlaid with appropriate media ...
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bioRxiv - Neuroscience 2024Quote: L4 transgenic worm larvae expressing ReaChR::mKate2 transgenes under the control of indicated promoters were transferred 12 hr before the assay to either normal E.coli OP50 plates or E.coli OP50-retinal plates containing 1 mM all-trans-retinal (ATR, Sigma). E.coli OP50-retinal plates were prepared by seeding 200 μl E.coli OP50 with 2 μl of 100 mM ATR in 100% ethanol ...
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bioRxiv - Plant Biology 2020Quote: ... Five small root or xylem pieces were plated on Malt Extract Agar (MEA) supplemented with 0.35 gl−1 of streptomycin sulphate (Sigma-Aldrich, St. Louis, MO, USA) (MEAS) ...
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bioRxiv - Neuroscience 2021Quote: ... The stability of recordings was improved by drainage of the cisterna magna and covering the exposed cortex with agar at 4% (Sigma-Aldrich, Cat#A7002-250G). The exact location of the probe was optimized by assessing the responses to tactile stimulation of the rat’s hindlimb with a cotton swab while listening to the recorded signal through a pair of loudspeakers.
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bioRxiv - Genetics 2020Quote: ... we arrayed multiple aliquots of 10μL of embryos suspended in 1X PBS onto a petri dish containing 2% agar dyed blue with Erioglaucine Disodium Salt (Sigma-Aldrich 861146; 8mg/mL).
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bioRxiv - Microbiology 2023Quote: ... One hundred microliters of the suspension were plated on tryptic soy agar 1/10 strength (10% TSA) supplemented with cycloheximide (50 µg.ml-1, Sigma-Aldrich, Saint-Louis, Missouri, USA). Plates were incubated at 20°C during a minimum 5 days ...
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bioRxiv - Cell Biology 2023Quote: ... . Ultrathin sections placed on grids were stained with 1% aqueous uranyl acetate (Agar Scientific, Stansted, UK) and 3% lead citrate (Sigma Aldrich, St. Louis, USA), and observed at the FEI Morgagni 268D microscope.
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bioRxiv - Neuroscience 2022Quote: ... The lumbar spinal cord from L1 - L6 was removed and embedded in 2.5% w/v agar (No. A-7002, Sigma-Aldrich, St Louis, MO, USA). The agar block was then superglued with Loctite 401 (Henkel Corporation ...
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bioRxiv - Plant Biology 2024Quote: Plant growth-promoting activity and pathogen protection were evaluated on Murashige and Skoog (MS) agar (MS basal media; Sigma-Aldrich, 0.03 % phytagel, pH 5.7) culture plates (11×11 cm square) ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was isolated from the aboveground tissues of seedlings using the Spectrum Plant RNA Isolation Kit (Sigma-Aldrich). The RNA was treated with RNase-free RQ1 DNase (Promega) ...
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bioRxiv - Neuroscience 2021Quote: ... After isolation 200,000 neurons were plated onto circular glass coverslips (Electron Microscopy Services) pre-coated with poly-L-lysine (Sigma) and mouse laminin (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... cut into smaller pieces and homogenize in 3 ml of nuclei isolation buffer (nuclei EZ lysis buffer (Sigma, NUC101), 1x Halt™ protease inhibitor cocktail (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were resuspended with 2% FBS in PBS for downstream B cell isolation or 10% Dimethyl sulfoxide (Sigma-Aldrich) in FBS for further preservation.
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bioRxiv - Physiology 2022Quote: Carotid bodies were incubated at 37°C for 25-30min in isolation medium consisting of: 0.125mg/ml Trypsin (Sigma), 2.5mg/ml collagenase Type 1 (Worthington ...
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bioRxiv - Cell Biology 2020Quote: Mitochondria were freshly isolated from IECs of Smyd5fl/fl and Smyd5ΔIEC mice as described before 20 using a mitochondria isolation kit (MITOISO1, Sigma) according to the manufacturer’s instruction.
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bioRxiv - Cancer Biology 2021Quote: Primary PSC isolation: pancreata were collected postmortem from Hsf1 null mice or WT littermates into HBSS (Sigma-Aldrich, H6648), then minced into Roswell Park Memorial Institute 1640 (RPMI ...
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bioRxiv - Developmental Biology 2022Quote: Nuclei from the 700mg of wildtype flower tissue were isolated using the Plant Nuclei isolation kit (Sigma, # CELLYTPN 1) conferring to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Nuclei were isolated from 50,000 cells for each sample using Nuclei EZ prep-Nuclei Isolation Kit (Sigma-Aldrich, USA). The transposition reaction mix (25μl of 2X TD buffer ...
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bioRxiv - Immunology 2022Quote: ... followed by peripheral blood mononuclear cell (PBMC) isolation according to the manufacturer’s instructions for Histopaque-1077 (Cat. No. H8889-500ML, Sigma). The buffy coat was collected ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was isolated from the tissues of 3DPG seedlings using the Spectrum Plant RNA Isolation Kit (Sigma-Aldrich). The RNA was treated with RNase-free RQ1 DNase (Promega) ...
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bioRxiv - Plant Biology 2023Quote: ... The pellet was resuspended in 10 mL isolation buffer and layered on 40% (w/v) Percoll® (Sigma-Aldrich) followed by centrifugation at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... or by mixing 70 µl swab elute or tissue homogenates with 630 µl TriPure Isolation Reagent (Sigma–Aldrich, 11667165001). Purification and RT-qPCR quantification of CCHFV RNA were conducted as previously described [64].