Labshake search
Citations for Millipore Sigma :
1301 - 1350 of 10000+ citations for 7 Bromo 2 chloromethyl 4H pyrido 1 2 a pyrimidin 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2022Quote: ... Bb was cultured at 37ºC in Barbour-Stonner-Kelly with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer media (BSK-H) complete with 6% rabbit serum (Millipore Sigma) and 1% Amphotericin B (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2019Quote: ... for 1 h at room temperature in the dark and incubated with 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich) for 10 min ...
-
bioRxiv - Neuroscience 2020Quote: ... To identify the total number of cells the nuclei were stained with DAPI (4’, 6-Diamino-2-phenylindole dihydrochloride) (1:10000, Millipore-Sigma). Images were acquired using Olympus BX61 microscope and analyzed using Fiji software (ImageJ).
-
bioRxiv - Neuroscience 2020Quote: ... The cells were centrifuged for 5 min at 700g and treated for 10 min with DAPI (4’,6-diamidino-2-phenylindole) (1:4000, Sigma, 62248) to label dead cells ...
-
bioRxiv - Immunology 2021Quote: ... the cells were fixed with 4% formaldehyde solution diluted in PBS during 2 hours and stained with 1% Naphtol blue black (Sigma, USA) solution for 1 hour for plaque visualization ...
-
bioRxiv - Developmental Biology 2020Quote: ... They were then rinsed 4 times with the same medium free of GFP-nanobodies and fixed at room temperature for 2 hours with PBS supplemented with 4% paraformaldehyde (PFA) and 1% BSA (A7638 Sigma-Aldrich). Open-books were observed with a STED microscope (TCS SP8 ...
-
bioRxiv - Immunology 2021Quote: ... cells were incubated for 20 minutes at room temperature with DAPI (4',6-diamidino-2-phenylindole; 1:150000, Sigma-Aldrich), mounted on glass slides with Fluoromount-G (Invitrogen ...
-
Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... DOI (1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane hydrochloride-hydrochloride) and serotonin were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). LP44 (4-[2-(methylthio)phenyl]-N-(1,2,3,4-tetrahydro-1-naphthalenyl)-1-piperazinehexanamide hydrochloride ...
-
bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
-
bioRxiv - Microbiology 2023Quote: ... Washed beads were adjusted to pH 7.5 with 200 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and bound proteins were reduced using 5 mM dithiothreitol (Sigma-Aldrich) at 37°C for 1 h ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were plated in serum-free growth medium (SFGM, Supplementary Table 1) on fibronectin-coated (4 μg cm-2 bovine fibronectin; F1141, Sigma-Aldrich) tissue cultureware.
-
bioRxiv - Bioengineering 2023Quote: ... Cells were plated in serum-free growth medium (SFGM, Supplementary Table 1) on fibronectin-coated (4 μg cm-2 bovine fibronectin; F1141, Sigma-Aldrich) tissue culture vessels ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 1 min with 4’,6-diamidino-2-phenylindole (DAPI; 0.5 μg/ml, Sigma-Aldrich, cat# D8417) diluted in PBS to label cell nuclei ...
-
bioRxiv - Molecular Biology 2024Quote: ... The transfection plasmids were mixed in a 4:2:1 ratio in Opti-MEM medium utilizing the polycationic agent polyethylenimine (PEI; Sigma-Aldrich). Following transfection ...
-
bioRxiv - Bioengineering 2024Quote: ... The samples were then washed three times in PBS and incubated with 4’-6-diamidino-2-phenylindole (DAPI) (1:1000; Sigma-Aldrich) at room temperature for 20 min ...
-
bioRxiv - Immunology 2024Quote: ... U-937 cells were maintained in RPMI-1640 medium supplemented with 4.5 g/L glucose, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1.0 mM sodium pyruvate (Sigma-Aldrich; Merck KGaA), 10% fetal bovine serum (Hyclone ...
-
bioRxiv - Microbiology 2024Quote: ... and influenza A/California/7/2009 H1N1pdm09 virus were amplified on MDCK α2-6 in MEM supplemented with 1% HEPES and 1 μg/ml trypsin treated with N-tosyl-L-phenylalanine chloromethyl ketone (Sigma, Oakville, ON, Canada). Viral titers were determined by plaque assays ...
-
bioRxiv - Immunology 2019Quote: ... 4h re-stimulation with PMA (50ng/mL) and ionomycin (1mM) (both Sigma) in the presence of Brefeldin A was carried out prior to fixation and permeabilization ...
-
bioRxiv - Genomics 2020Quote: ... 2 mM DTT) by performing buffer exchange three times using one Amicon 30 kDa MWCO spin column (Millipore UFC903008). The purified protein was then transferred to a new tube ...
-
bioRxiv - Developmental Biology 2021Quote: Global transcription block was performed either by treatment with 2 μM triptolide from the one-cell stage (Sigma T3652) in E3 media ...
-
bioRxiv - Microbiology 2020Quote: ... T cells were then infected with HIVBaL and cultured for up to 7 days in RPMI medium additionally supplemented with 10 U/ml recombinant interleukin-2 (Sigma). The dividing cell lines HEK293T (ATCC ...
-
bioRxiv - Neuroscience 2020Quote: ... 80-05-7), and paracetamol (purity >99.0 %, 103-90-2) and corn oil (as a control vehicle) were obtained from Sigma-Aldrich. EDC compounds were dissolved in corn oil in order to obtain the final concentration showed in Table 1.
-
bioRxiv - Cancer Biology 2021Quote: ... 150 000 cells in 100 μL of flow cytometry buffer were stained with 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Sigma-Aldrich) for 15 min at 37°C ...
-
bioRxiv - Plant Biology 2020Quote: Plants were fumigated for 30 min with 50 μM 2’,7’-dichlorofluorescein diacetate (H2DCFDA, Millipore-Sigma, St. Louis, MO, USA) in a glass container using a nebulizer (Punasi Direct ...
-
bioRxiv - Plant Biology 2020Quote: Plants were fumigated for 30 min with 50 μM 2’,7’-dichlorofluorescein diacetate (H2DCFDA, Millipore-Sigma, St. Louis, MO, USA) in a glass container using a nebulizer (Punasi Direct ...
-
bioRxiv - Bioengineering 2022Quote: ... sections were washed in tap water for 2 minutes and counterstained for 7 minutes in Nuclear Fast Red stain (0.1% Nuclear Fast Red [Sigma Aldrich, St Louise ...
-
bioRxiv - Neuroscience 2019Quote: ... 5, 6 and 7 (days 52 to 55 in the overall timeline, Figure 2) at 100 mg/kg ip (Sigma), at approximately 17:00 hr ...
-
bioRxiv - Plant Biology 2020Quote: ... samples were resuspended in Urea 7 M and Thiourea 2 M buffer and desalted on Amicon Ultra-0.5 3 kDa centrifugal filters (Merck Millipore, Germany). Filters were filled to maximum capacity with buffers and centrifuged at 15,000 g for 10 min at 20°C ...
-
bioRxiv - Immunology 2022Quote: ... Plates were incubated at 37 degrees C in 5% CO2 for 7 days before fixing with 2% formaldehyde and staining with 0.1% crystal violet (Sigma-Aldrich).
-
bioRxiv - Plant Biology 2023Quote: ... plants were fumigated for 30 min with 50 µM 2′,7′-dichlorofluorescein diacetate (H2DCFDA; Millipore-Sigma, St. Louis, MO, USA) for ROS imaging ...
-
bioRxiv - Plant Biology 2023Quote: ... plants were fumigated for 30 min with 50 µM 2′,7′-dichlorofluorescein diacetate (H2DCFDA; Millipore-Sigma, St. Louis, MO, USA) for ROS imaging ...
-
bioRxiv - Cancer Biology 2023Quote: ... The eluted M-protein fractions were collected in Eppendorf tubes prefilled with 5 μl 2 M TRIS.HCl (pH=7, Sigma Aldrich), to neutralize the solution ...
-
bioRxiv - Immunology 2023Quote: Tumors were digested for 45 minutes with gentle shaking at 37°C in HBSS containing 7 mg/ml Collagenase D and 2 mg/ml DNase I (Sigma). Single-cell suspensions were stained with fluorophore-conjugated antibodies obtained from BioLegend ...
-
bioRxiv - Developmental Biology 2023Quote: Oxidative stress was quantified by measuring total reactive oxygen species (ROS) using 2μM 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA; Sigma-Aldrich) staining and FACS analysis ...
-
bioRxiv - Bioengineering 2023Quote: ... Protein-containing fractions were pooled and buffer-exchanged against HBS-E (10 mM HEPES at pH 7, 150 mM NaCl, 2 mM EDTA) by ultrafiltration using Amicon Ultra devices (Millipore).
-
bioRxiv - Neuroscience 2023Quote: Non-anesthetized 7 dpf Tg(HuC:H2B-GCaMP6F; casper) larvae were immobilized at in 2% low melting point agarose (Sigma-Aldrich) prepared in Danieau solution and mounted dorsal side up in a custom-made perspex cylindrical Danieau-filled chamber ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were then treated with 16 μM of the ROS-sensitive fluorogenic probe 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA, Sigma-Aldrich) for 1 h in the dark at 30°C ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... SAG1.3 (3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide) was from Sigma. Cyclopamine-KAAD and SANT-1 ((4-Benzyl-piperazin-1-yl)-(3,5-dimethyl-1-phenyl-1H-pyrazol-4-ylmethylene)-amine ...
-
A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: Cells were seeded on coated glass cover (Assistent) slips 2 days before fixation with 4 % paraformaldehyde (Sigma, 30525-89-4) for 15 minutes ...
-
bioRxiv - Immunology 2021Quote: PBMCs and tissue MNC suspensions were treated with 5 μM Chk2 inhibitor II or 2-[4-(4-chlorophenoxy)phenyl]-1H-benzimidazole-5-carboxamide (Sigma) prepared in final volume of 1% bovine serum albumin (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2023Quote: ... After addition of 250 µL 4× lysis buffer (4% SDS, 40 mM tris(2-carboxyethyl)phosphine (TCEP; Sigma-Aldrich, C4706), 160 mM chloroacetamide (CAM ...
-
bioRxiv - Microbiology 2023Quote: ... inoculum was replaced with serum-free medium supplemented with 1 µg/ml tosyl phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (Sigma) and 0.14% BSA fraction V (w/v ...
-
bioRxiv - Molecular Biology 2019Quote: ... the collagenase inhibitor Ilomastat ((R)-N′-Hydroxy-N-[(S)-2-indol-3-yl-1-(methylcarbamoyl)ethyl]-2-isobutylsuccinamide) (25μM, CC1010, Merck Millipore) or a combination of both ...
-
bioRxiv - Microbiology 2021Quote: ... pellets were resuspended in 2 ml 10 mM Tris HCl (pH 7.4) containing 2 mg ml−1 pronase (Sigma). Tubes were incubated for 3 h at 60 °C to digest proteins ...
-
bioRxiv - Cancer Biology 2020Quote: Both pBabe-SAOS 2 and hTERT-SAOS 2 (107 cells) were cross-linked with 1% paraformaldehyde (Sigma Aldrich, # P6148) in culture medium for 10 min at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: ... treated with poly-d-lysine(Sigma-Aldrich A-003-E, 1-2 hr at 37°C, 2 μL/well) followed by laminin (Thermo Fisher 23017015 ...
-
bioRxiv - Immunology 2021Quote: ... cells were stimulated for 4h at 37°C with PMA (phorbol myristate acetate; 0.5 μg mL-1; Sigma Aldrich) and ionomycin (1 μg mL-1)(Sigma Aldrich ...
-
bioRxiv - Neuroscience 2022Quote: ... were dissected as mentioned above and fixed with 2% paraformaldehyde and 0.06% glutaraldehyde in PEM (0.1 M PIPES: P1851-500g, Sigma, Germany; 2 mM EGTA: E3889, Sigma, Germany; 1 mM MgSO4 x 7 H2O, v/v) for 90 min on ice ...
-
Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... and (2′S)-2′-Deoxy-2′-fluoro-5-ethynyluridine (F-ara-EdU) were purchased from Sigma-Aldrich, dissolved in water ...
-
Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... and stained with 200 μM 7-Azido-4-Methylcoumarin (AzMC) (Sigma). The stained cells were mounted on a glass slide and visualized using Leica TCS SP5 confocal microscope (excitation ...