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Citations for Millipore Sigma :
1151 - 1200 of 10000+ citations for 7 Bromo 2 chloromethyl 4H pyrido 1 2 a pyrimidin 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... then rinsed and counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (D9542, Sigma□Aldrich). The same IF protocol was applied to the other antibodies ...
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bioRxiv - Pathology 2022Quote: ... and nuclei in the cells were counterstained with 4’,6-diamidino-2-phenylindole (Sigma). The samples were examined by using an epifluorescence microscope (Olympus ...
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bioRxiv - Microbiology 2023Quote: ... 100 µM 2’(3’)-O-(4-Benzoylbenzoyl) adenosine 5’-triphosphate (BzATP) (#B6396, Sigma-Aldrich), 20 µM MCC950 (#inh-mcc ...
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bioRxiv - Systems Biology 2023Quote: ... After labeling the nuclei with 4’,6-diamid-ino-2-phenylindole (DAPI; Sigma-Aldrich), the cells were mounted with Mowiol 40-88 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... and DAPI (4=,6-diamidino-2-phenylindole) were purchased from Sigma (St. Louis, MO). MG132 was purchased from Calbiochem (San Diego ...
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bioRxiv - Bioengineering 2023Quote: ... and collagen were stained with 4′,6-diamidino-2-phenylindole (DAPI (Sigma-Aldrich, D9542), diluted to 0.1 μg/ml in PBS) ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were stained with either DAPI (4′,6-diamidino-2-phenylindole, Sigma-Aldrich F6057) for glass coverslip or Hoechst 33342 (ThermoFisher 62249 ...
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bioRxiv - Immunology 2023Quote: ... additional staining with 4’,6-diamidino-2-phenylindole (DAPI, 100 ng/ml; Sigma-Aldrich) was performed ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were counterstained with a 4’,6-diamidino-2-phenylindole solution (DAPI; Sigma-Aldrich). After four washes with TBST ...
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bioRxiv - Genetics 2024Quote: ... and incubated for 2 hours at 4°C with anti-FLAG M2 antibody (Sigma)-coupled Dynabeads (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were stained with 0.0001% of 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich) and cover slips were mounted using fluorescent mounting medium (DAKO) ...
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bioRxiv - Cancer Biology 2024Quote: ... The DNA content was assessed by DAPI (4′,6-diamidino-2-phenylindole; Sigma-Aldrich) staining ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% fetal bovine serum (FBS) and 1 ng/ml fibroblast growth factor-2 (FGF-2; Millipore). Cells were grown in tissue culture treated 24 well plates and 5% CO2 at 37°C.
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bioRxiv - Bioengineering 2021Quote: ... 1.5 µL g-1 solution of the photoinitiator 2-hydroxy-2-methyl propiophenone (Irgacure 1173; >97 %, Sigma-Aldrich) was added at 4°C temperature ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM lipids: POPC: POPE: cardiolipin with a 2:2:1 weight ratio) and 160 μL MSP1D1 (Sigma, 5 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... TEBP-1-His5 and TEBP-2-His5 were expressed in Rosetta 2 (DE3) pLysS Competent Cells (Novagen,#71401). An overnight culture was grown in LB containing the respective antibiotic ...
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bioRxiv - Bioengineering 2020Quote: ... 1.5 μL g−1 hydrogel of the photoinitiator 2-hydroxy-2-methyl propiophenone (Irgacure 1173; >97 %, Sigma-Aldrich) were mixed in at a temperature of 4 °C ...
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bioRxiv - Biophysics 2020Quote: ... samples were incubated with 2 % (v/v) primary anti-tubulin antibody (clone B-5-1-2, Sigma-Aldrich) in blocking buffer for 60 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 150 μM (2-(2-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl) acetamide (EF5 compound; Sigma-Aldrich, EF5014), 0.5 μM MitoSOX™ fluorescent probe (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: Metabolic quenching and nucleotide phosphate metabolite pool extraction were performed by adding 1 ml ice-cold 2:2:1 acetonitrile:methanol:water with 0.1% formic acid with 1x MS-Stop phosphatase inhibitor (Sigma-Aldrich). Samples were spiked with 10 µl 100 mM mix of adenosine-15N5-5′-triphosphate ...
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bioRxiv - Microbiology 2023Quote: ... Cell-laden filters were suspended in 2-ml bead beater tubes (SSIbio, # 21276) containing 1 ml of fresh metabolite extraction solvent (2:2:1 ratio of acetonitrile (Sigma-Aldrich, # 900667), methanol (>99.8%) ...
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Proteasome granular localization is regulated through mitochondrial respiration and kinase signalingbioRxiv - Cell Biology 2022Quote: ... and 2-[2-(3-chlorophenyl)hydrazinylidene]-propanedinitrile (CCCP) (Sigma), were used at final concentrations of 0.5 μM ...
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bioRxiv - Microbiology 2019Quote: ... 2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris; Sigma Aldrich), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES ...
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bioRxiv - Immunology 2019Quote: ... 2 mM Glutamax and 50 μM 2-mercaptoethanol (Sigma) (all from Thermo Fisher unless stated otherwise).
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bioRxiv - Cell Biology 2019Quote: ... Around 2–5 μl of 2 mM levamisole (Sigma) was pipetted out on the center of the agarose pads ...
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bioRxiv - Developmental Biology 2020Quote: ... 616452 (2 μM, Sigma-Aldrich, no. 446859-33-2) and penicillin (100 U/mL ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1 mM 2-mercaptoethanol (2-ME; Sigma-Aldrich, USA), and 4 ng/ml FGF-2 (PeproTech ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... 2-aminoethoxydiphenyl borate (2-APB; Sigma Aldrich, Oakville, ON) experiments ...
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bioRxiv - Biophysics 2021Quote: ... precut 2×2-well culturewell gasket (GBL103250, Sigma, MO) was attached to the coverslip ...
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bioRxiv - Cell Biology 2020Quote: ... 2% (v/v) phosphatase inhibitor cocktail 2 (Sigma-Aldrich) and 500 U/ml universal nuclease ...
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bioRxiv - Immunology 2021Quote: ... plus 2 % FBS and 2 mM EDTA (Sigma-Aldrich) (from now on termed “staining buffer”) ...
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bioRxiv - Immunology 2021Quote: ... 2 mM Glutamax and 50 μM 2-mercaptoethanol (Sigma) (all from Thermo Fisher unless stated otherwise).
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bioRxiv - Biochemistry 2022Quote: ... 2-[2-(3-chlorophenyl)hydrazinylyidene]propanedinitrile (CCCP) (Sigma-Aldrich), rhodamine 123 ...
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bioRxiv - Neuroscience 2023Quote: ... 616452 (2 μM, Sigma-Aldrich, no. 446859-33-2) and penicillin (100 U/mL ...
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bioRxiv - Microbiology 2023Quote: ... ≥95% pure 2’-C-methylcytidine (2’CMC; Sigma, M4949) was serially diluted in 0.5 log10 increments in Milli-Q H2O ...
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bioRxiv - Microbiology 2022Quote: ... 2-deoxy-D-glucose (2-DG, Sigma-Aldrich, D8375), D-(+)-mannose (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Samples were labeled using 2-aminobenzamide (2-AB, Sigma) for 2 hours at 65°C ...
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bioRxiv - Immunology 2023Quote: ... Recombinant human (rh) Interleukin-2 (IL-2, Sigma-Aldrich), rhTNFα (Peprotech ...
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bioRxiv - Biophysics 2023Quote: ... 2-aminoethoxydiphenyl borate (2-APB) (Sigma-Aldrich/Merck, Germany), BL-1249 ...
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bioRxiv - Developmental Biology 2023Quote: ... and 200µM of 2-Mercaptoethanol (2-ME; Sigma-Aldrich).
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bioRxiv - Developmental Biology 2020Quote: ... and then incubated with nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) substrates (Sigma-Aldrich) in NTM (100mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... and the blotting signals were chemically visualized with either the nitro-blue tetrazolium/5-bromo-4-chloro-3′-indolyphosphate (NBT/BCIP) chromogenic assay (Sigma) or infrared scanner ...
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bioRxiv - Plant Biology 2020Quote: ... plants were incubated overnight at 37°C in GUS buffer (3 mM 5-bromo-4-chloro-3-indolyl-β-D-glucuronide [Duchefa Biochemie, Haarlem, The Netherlands], 0.1% v/v Triton X-100 [Sigma, Steinhaim ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... coli DH5α cells were transformed with the resulting pho-lac fusion plasmids and streaked on dual indicator plates containing LB agar with 5-bromo-4-chloro-3-indolyl phosphate disodium salt (X-Phos) (Sigma) at a final concentration of 80μg/ml and 6-chloro-3-indolyl-β-D-galactoside (Red-Gal ...
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bioRxiv - Microbiology 2021Quote: ... Immunoreactive bands were detected by the addition of BCIP (5-bromo-4-chloro-3-indolylphosphate)-nitroblue tetrazolium solution (Sigma-Aldrich). The reaction was stopped after 2 min by washing the blots with large volumes of deionized water.
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bioRxiv - Bioengineering 2021Quote: ... slides were either treated with SIGMA FAST™ BCIP/NBT (5-Bromo-4-chloro-3-indolulphosphate/Nitro blue tetrazolium, pH 9.5, Sigma) and counterstained with nuclear fast red (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... Color was developed with freshly prepared 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium (BCIP/NBT; Sigma-Aldrich) and the reaction was stopped by addition of 2 mM ethylenediaminetetraacetic acid (EDTA).
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bioRxiv - Microbiology 2023Quote: ... the blots were washed again using TBST and developed using AP-reactive Nitrobluetetrazolium (NBT) 5-bromo-4-chloro-3-indolylphosphate (BCIP) tablets (Sigma). A final image of the blots was taken on a Bio-Rad ChemiDoc gel imaging system using colorimetric detection.
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bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated in NBT (nitro-tetrazolium blue)-BCIP (5 bromo-4-chloro-3’-indolyphosphate p-toluidine) (Sigma-Aldrich), 0.033% in APTMg in dark condition ...
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bioRxiv - Immunology 2022Quote: ... Perturbation profiling of the use of metabolic pathways by T cells was achieved by the addition of oligomycin (O4876; 1 µM), Carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920; 2 µM) and rotenone (R8875; 1 µM - all from Sigma Aldrich, St. Louis, MO). Metabolic parameters were then calculated based on the following formulas: