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Citations for Millipore Sigma :
1251 - 1300 of 1367 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Protein extraction was performed under denaturing conditions (50 mM TRIS, 150 mM NaCl, 1 mM EDTA, 1% SDS) containing protease (Sigma-Aldrich #11836145001) and phosphatase inhibitors (Sigma-Aldrich #P5726-5ML ...
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bioRxiv - Pathology 2021Quote: ... (groups 2 & 3) treated groups including mice receiving daily intraperitoneal injection of 150 mg/kg/day of β-aminopropionitrile fumarate (BAPN, A3134; Sigma Aldrich) for 28 days ...
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bioRxiv - Developmental Biology 2020Quote: ... 1:500), Rabbit anti-phospho Chk1 (CST, 1:200), Rabbit anti-phospho Smad (CST, 1:150) Rabbit anti-pH3 (Millipore, 1:500), and Alexa 488/568/647-conjugated Donkey anti-Chicken/Rabbit/Mouse secondary antibodies (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: Crystal violet stain that had incorporated into the biofilm was re-solubilised upon the addition of 150 μL of 1% sodium dodecyl sulphate (SDS) (w/v) (Sigma-Aldrich, USA).
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bioRxiv - Cell Biology 2021Quote: ... and incubated for 1 h at 37°C with gentle rotation (20 rpm) in balanced salt solution (BSS) media containing 150 U/ml collagenase type IV (Sigma-Aldrich C5138), 60 U/ml DNase I (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated for 1 hour at 37°C with gentle rotation (20 rpm) in balanced salt solution (BSS) media containing 150 U/ml collagenase type IV (Sigma-Aldrich C5138), 60 U/ml DNase I (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: Animals were anesthetised by intraperitoneal injection of pentobarbital (150 mg/Kg) and were transcardially perfused with 20 mL oxygenated aCSF followed by 60 ml 2% paraformaldehyde (PFA; Millipore-Sigma; P6148) (in 2X Phosphate-buffered saline (PBS) ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight at 4 °C with BB containing a primary antibody cocktail (1:150 mouse anti-ionized calcium-binding adapter molecule 1 (Iba1; EMD-Millipore cat# MABN92+), 1:300 rabbit anti-transmembrane protein 119 (TMEM119 ...
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bioRxiv - Biochemistry 2023Quote: ... dried phosphopeptides from each fraction were re-dissolved in 800 μL cold IAP buffer (50 mM Tris-HCl, 150 mM NaCl, 1% w/v n-Octyl-β-D-glucopyranoside (EMD Millipore), EDTA-free Halt protease inhibitor and phosphatase inhibitor cocktails ...
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bioRxiv - Neuroscience 2022Quote: ... Homogenization was performed using a glass-Teflon homogenizer in 1 ml of homogenizing buffer (50 mM HEPES, 150 mM NaCl, 1 mM EDTA + mammalian protease inhibitor cocktail (Sigma CAT# P8340)) with 30 slow strokes on ice ...
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bioRxiv - Biochemistry 2024Quote: ... were mixed with 2µL ATP/ CDK2 substrate mix to make final concentration of 150 µM ATP and 0.5 µg substrate per reaction (Substrate used is either histone H1(Sigma-Aldrich, 14-155) or RB1 C-terminal peptide (MyBioSource ...
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bioRxiv - Neuroscience 2024Quote: ... Then the coverslips are placed in a closed chamber with 150 µl of (3-aminopropyl) triethoxysilane (Sigma-Aldrich, Saint-Quentin Fallavier, France) and a desiccator (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... The NC and KO wells received 150 uL of Tyrode’s supplemented with probenecid with 800 nM capsaicin (Sigma M2028-50MG, Lot SLCB0726) while the non-stimulated control well received only Tyrode’s supplemented with probenecid ...
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bioRxiv - Microbiology 2024Quote: ... was grown (~25°C) in TB medium with 2% DMSO with or without 150 mg/L acyclic naphthenic acids (Sigma Aldrich 70340,15), or 150 mg/L of custom mix of 9X naphthenic acids (Table S1) ...
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bioRxiv - Neuroscience 2024Quote: ... cultures were washed in PBS and protein harvested in lysis buffer (150 mM sodium chloride, 10 mM sodium fluoride, 1% IGEPAL CA-630, 1% protease inhibitor cocktail (Sigma-Aldrich P8340), 1% phosphatase inhibitor cocktail 2 (Sigma-Aldrich P5726) ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then gently washed three times with Tris-buffered saline (TBS)-Tween (150 mM NaCl, 50 mM Tris-HCL buffer and 1% w/v Tween20) (TBS; EMD Millipore 524750, Tween20; Sigma-Aldrich) and incubated in Alexa Fluor 680 Donkey anti-goat secondary (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: Cells were washed once with cold PBS and 1.2 ml of lysis buffer (Tris 50 mM; NaCl 150 mM; pH 7.3; Triton X-100 1 % (Sigma-Aldrich. Cat. Nº: T9284); Leupeptin 5 µM (Focus Biomolecules ...
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bioRxiv - Cell Biology 2022Quote: Cell samples were collected using an NaCl (150 mM)-EDTA (10 mM) buffer with added proteinase inhibitor cocktail 1:100 (Sigma-Aldrich, France) and then denaturated in Laemmli denaturating buffer 4X ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then induced in SGDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% galactose [Sigma-Aldrich #G0625] ...
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bioRxiv - Physiology 2023Quote: ... mice were fasted for 6 hours followed by oral gavage of 150 µL of 80 mg/mL FITC-dextran (Sigma, 4 kDa). Blood was collected via tail vein at baseline and 1-hour post-gavage ...
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bioRxiv - Cancer Biology 2023Quote: Total cell lysates were extracted from adherent cells by incubating the cells for 10 minutes on plate in ice cold cell lysis buffer (150 mM NaCl (Sigma-Aldrich: S3014); 1 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... single GC B cells were sorted into wells and 150 μl of supplemented OptiMEM along with 30 μg/ml LPS (Sigma-Aldrich, #L6511) and 4 ng/ml IL-4 (Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... buffer (25 mM Tris-HCl, pH 7.5 [Sigma-Aldrich], 150 mM NaCl [JT Baker], 1% NP-40 [Sigma-Aldrich], 1% sodium deoxycholate [Sigma-Aldrich] ...
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bioRxiv - Neuroscience 2024Quote: ... then clarified (3000g x 10min) and concentrated to approximately 150 µL by using an Amicon Ultra-15 centrifugal filter unit (NMWL 100 kDa, Sigma #Z740210-24EA) at 5000g for 30-60 min ...
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bioRxiv - Microbiology 2024Quote: ... The combined 150 mL of buffer with resuspended biomass was filtered onto a Stericup vacuum filter (Millipore S2VPU02RE, 0.1 µm pore size), and the filter membrane was cut into several pieces using a sterile scalpel ...
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bioRxiv - Microbiology 2024Quote: ... 2D PNOs monolayers were washed 2 times with 150 µl of PBS and were lysed with 1 % Saponin from Quillaja Bark (Sigma S7900-25G) to assess the associated bacteria ...
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bioRxiv - Immunology 2024Quote: Biopsies were homogenized in Eve Technologies’ recommended protease inhibitor buffer (20 mM Tris HCl, 0.5% Tween 20, 150 mM NaCl, cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail (Sigma-Aldrich, 11836170001) in diH20 ...
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bioRxiv - Cell Biology 2024Quote: Cells in 6-well plates were treated as described in figure legends before lysis in 150 µl ice-cold RIPA buffer including protease (Sigma Millipore 539131-1VL) and phosphatase (Sigma Millipore 524627 ...
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bioRxiv - Molecular Biology 2024Quote: ... For a positive ribosome collision control 28 75-90% confluent HEK293 cells were cultured in 150 mm dishes and incubated with medium containing 1.8 µM Emetine (Millipore, Cat. No. 324693) for 15 minutes at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate mixture was cooled to room temperature for 10 min and then neutralized by adding 150 µL 0.5M Trizma base (Millipore-Sigma, Cat # T1503). For measuring NADH/NADPH ...
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bioRxiv - Molecular Biology 2024Quote: ... To enrich Mek-Raf complexes, 150 μL of supernatant was combined with 12 μL anti-flag beads (50% slurry, washed, (Sigma cat#M8823)) and rotated end-over-end at 4°C for 1 h then quickly washed three times with 100 μL modRIPA ...
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bioRxiv - Genomics 2024Quote: ... Each coverslip was aspirated dry and hybridized with 30uL of encoding solution containing 600 ug of RNA encoding probes (150 ug/ section) in 50% (v/v) formamide with 10% (w/v) dextran sulfate (Sigma Aldrich, D6924) and 1xTBS and 0.1% Tween-20 and 3g/mL PVSA ...
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bioRxiv - Systems Biology 2024Quote: ... 5 μL of each metabolite sample was injected onto a SeQuant ZIC-pHILIC column 5 μm polymer 150 × 2.1 mm (Millipore/Sigma, Cat# 1504600001). Buffer A was 100% acetonitrile ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were washed three times with Tris-buffered saline (TBS)-Tween (150 mM NaCl, 50 mM Tris-HCL buffer and 1% w/v Tween20) (TBS; EMD Millipore 524750, Tween20; Sigma-Aldrich) followed by secondary antibody and Hoechst 33342 (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... The pellets were then lysed in RIPA lysis buffer (50 mM Tris, 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1% Triton X-100, protease cocktail [Sigma-Aldrich], and 1 mM PMSF) for 5 minutes at 4°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... 150 mM NaCl) containing a protease and phosphatase inhibitor cocktail (cOmplete™, EDTA-free Protease Inhibitor Cocktail; PhosSTOP™ inhibitor tablets, Millipore Sigma). Subcellular protein fractionations were performed using the NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Conventional immunocytochemical staining was done to quantify the activated PAK1 and actin in oAβ and IPA-3 treated cells using phospho-PAK1 (Thr423)/PAK2 (Thr402) antibody (Cell signalling #260; 1:150) and Phalloidin–Tetramethylrhodamine B isothiocyanate (Sigma P1951, 1:500). Anti-rabbit Alexa 405 and FITC (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated in rabbit anti-choline acetyltransferase (ChAT, the synthetic enzyme for acetylcholine) for three days at 4°C (1:150; Cat#AB143, EMD Millipore, Billerica, MA). After incubation ...
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bioRxiv - Microbiology 2021Quote: ... followed by horseradish peroxidase (HRP)-conjugated anti-mouse antibodies (Jackson, 715-035-150, 1:10000) or anti ß-actin-HRP (A3854, Sigma, 1:100000) antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... The flow-through containing native peptides was offline fractionated using alkaline C4 reverse phase chromatography (Kromasil® C4 HPLC column, 100 Å pore size, 3.5 μm particle size, length × I.D 150 × 2.1 mm, K08670362, Sigma-Aldrich, St. Louis, MO, USA) and each collected fraction was analyzed using the Orbitrap Fusion mass spectrometry system (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: Stock concentration of VSV-SARS-CoV-2 at a concentration of ~150 µg/mL viral RNA was conjugated with Atto565-NHS ester (Sigma-Aldrich, cat.72464) as previously described (32 ...
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bioRxiv - Neuroscience 2020Quote: ... On the day of the experiment the cells were placed on ice and washed 3 times with PBS supplemented with 10mM EDTA (Sigma, 150-38-9) for 3 minutes each ...
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bioRxiv - Biophysics 2021Quote: Methanol-fixed infected cells and a methanol-fixed negative control were incubated overnight at 4 C° with 150 μl of a solution of anti-S IgG or anti-N IgG in PBS + 3% BSA (Sigma-Aldrich, Milan, Italy). For colocalization experiments ...
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bioRxiv - Microbiology 2022Quote: Stock solutions of VSV-SARS-CoV-2 and its variants at a concentration of ∼150 µg/ml viral RNA were conjugated with Atto 565-NHS ester (Sigma-Aldrich, cat. 72464) as previously described (41) ...
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bioRxiv - Biochemistry 2020Quote: ... the cells were collected after 24-hr transfection in detergent-free buffer (40 mM HEPES, pH8.0, 150 mM NaCl, 10 mM β-glycerophosphate, 10 mM sodium pyrophosphate, 2 mM EDTA, 1x Sigma protease inhibitor cocktail). The cells were lysed by sonication for 3 seconds on ice followed by ultracentrifugation at 90,000xg in a TLA100.3 rotor for 1 hour at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... was conducted on an Ascentis Express RP-Amide column (150-mm length, 4.6-mm internal diameter, and 2.7-μm particle size; Supelco, Sigma-Aldrich, St. Louis, MO, USA), equipped with the appropriate guard column ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were placed back in the slide holder and 150 µL of the primary antibody (anti-NeuN Mouse, EMD Millipore Corp., #MAB377) diluted in RNAscope® Co-Detection Antibody Diluent (Advanced Cell Diagnostics ...
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bioRxiv - Plant Biology 2022Quote: ... resuspended in 2 to 5mL of room temperature infiltration medium (10 mM MgCl2, 10 mM MES KOH, pH 5.6, 150 mM acetosyringone [Sigma Aldrich], and 1% DMSO), diluted to a working optical density of 1 ...
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bioRxiv - Biochemistry 2023Quote: ... After 24 hours the media was replaced with fresh media and after 48 hours cells were treated with either 1 µg/mL blasticidin (Fisher, R21001) or 150 µg/mL hygromycin (Sigma-Aldrich, H7772-1G) and maintained in selection media until all uninfected control cells died ...
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bioRxiv - Systems Biology 2024Quote: Brain cortices were homogenized with a motorized pestle in RIPA buffer without SDS (30 mg tissue: 150 μL) with protease (P2714, Millipore, Bedford, MA, USA) and phosphatase (78427 ...