Labshake search
Citations for Millipore Sigma :
1251 - 1300 of 10000+ citations for Human Tropomyosin alpha 4 chain TPM4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2022Quote: ... Leptin content in media was analyzed by ELISA (Cat. # EZML-82K, Millipore Sigma, Danvers, MA).
-
bioRxiv - Microbiology 2022Quote: ... Biotinylated proteins were captured in ELISA plates precoated with 5 μg/ml of avidin (Sigma) in Na2CO3-NaHCO3 buffer (pH 9.6 ...
-
bioRxiv - Physiology 2023Quote: ... and VEGF concentrations were measured by ELISA (R&D Systems, Mercodia, Abcam, and Sigma, respectively), and total plasma bile acids using a colorimetric assay (Abcam) ...
-
bioRxiv - Biochemistry 2023Quote: ... diluted 1:10,000 in ELISA buffer (PBS, 5% Tween20, 1% goat serum (Sigma-Aldrich, G9023)) was added to all wells ...
-
bioRxiv - Synthetic Biology 2023Quote: ... were diluted in PBS and used to coat MaxiSorp 96-well ELISA plates (Sigma; M9410) for 1hr at room temperature ...
-
bioRxiv - Immunology 2024Quote: ... Nunc MaxiSorp ELISA plates were precoated with dsDNA (100 mg/mL, Sigma, St. Louis, MO) in phosphate-buffered saline (PBS ...
-
bioRxiv - Genetics 2019Quote: ... Expression of Scc1(R180D,268D)-HA was induced 1h after starting alpha-factor treatment by addition of newly-made galactose (Sigma-Aldrich, G0750) (2% final ...
-
bioRxiv - Biochemistry 2020Quote: ... The material was transferred into no-stick microtubes (Alpha Laboratories, cat. LW2410AS) and supplemented with 150 U/ml of Benzonase nuclease (Millipore, cat. 71206). Samples were incubated on a rotating wheel for 30 min at 4°C and centrifuged (16,000g ...
-
bioRxiv - Cell Biology 2022Quote: ... Alpha smooth-muscle actin (αSMA) and collagen I (col1) were stained using monoclonal antibodies (1:1000 diluted, Sigma, A2547 and SAB4200678, respectively). For detection of primary antibodies ...
-
bioRxiv - Plant Biology 2019Quote: ... Both vectors are Gateway® compatible and instructions outlined by Gateway® Technology were followed to complete the LR reaction and transformed in SIG10-5 alpha chemically competent cells (Sigma-Aldrich) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... membranes were then cut based on size and incubated overnight with the following primary antibodies: 1 μg/mL mouse α-alpha-tubulin (EMD Millipore), 1 μg/mL mouse mAb414 (Biolegend) ...
-
bioRxiv - Microbiology 2022Quote: ... The cells were activated with a cocktail of T-cell activators consisting of 40 ng/ml tumor necrosis factor-alpha (TNF-α; Catalog no. T0157; Sigma-Aldrich), 40 ng/ml phorbol myristate acetate (PMA ...
-
bioRxiv - Molecular Biology 2022Quote: ... RNA/DNA and alpha/beta tubulin were tested using cellulose beads with immobilized single and double stranded calf thymus DNA (Sigma Aldrich, USA). 1 µg of PfCoSP was mixed with 500 µl cellulose matrix (1mg/ml ...
-
bioRxiv - Physiology 2023Quote: ... Immunohistochemical (IHC) staining for smooth muscle actin was carried out using anti alpha-Smooth Muscle-Cy3 monoclonal antibody C6198 (Sigma-Aldrich Co.).
-
bioRxiv - Cell Biology 2023Quote: ... Cells were blocked in 3% BSA for 30 min at RT and incubated with anti-alpha-SMA antibody (1:1000; Sigma, Cat. # A2547) and anti-collagen type I antibody (1:1000 ...
-
bioRxiv - Cell Biology 2023Quote: ... The stripped membrane was re-blotted for a reference protein using 1:5000 monoclonal mouse anti-mouse alpha-tubulin (Sigma, T6199-25UL), overnight at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... C4-12 cells were cultured in phenol free minimum essential medium (MEM, Fisher #A10488-01) alpha media supplemented with 10% charcoal stripped serum (CSS; Sigma-Aldrich #F6765). Cell lines are maintained at 37°C and 5% CO2 ...
-
bioRxiv - Biophysics 2024Quote: ... Triton-X 100 in 1x PBS) for 10 min after which it was incubated with primary antibody solution (anti-alpha-tubulin, #T5168, Sigma-Aldrich, Germany) in IF buffer diluted 1:500 for 1.5 h at room temperature while shaking followed by washing thrice with 1x PBS ...
-
bioRxiv - Bioengineering 2019Quote: ... Lyophilized proteins were purchased: human serum albumin (HSA; from human plasma, ≤0.02% Fatty acids, Lot #SLBZ2785, Millipore Sigma) and fibrinogen (FBG ...
-
bioRxiv - Biochemistry 2021Quote: Lyophilized human haptoglobin phenotype 1-1 (Hp) and human α,β haemoglobin (Hb) were purchased from Sigma Aldrich. Deglycosylation enzyme PNgase F was from New England BioLabs Inc ...
-
bioRxiv - Neuroscience 2020Quote: ... and COs derived from H9 hESCs and iPSCs were measured using the Human Oxidative Phosphorylation Magnetic Bead Panel Milliplex MAP Kit (Millipore–H0XPSMAG-16K), following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... were measured with a human cytokine/chemokine panel utilizing Luminex 200 xMap technology and the MILLIPLEX MAP ® multiplex kit (Luminex xMAP technology; EMD Millipore, Chicago ...
-
bioRxiv - Pathology 2021Quote: ... Cytokine and chemokine levels in monkey sera were measured using a MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Premixed 23 Plex - Immunology (Milliplex MAP kit, Merck Millipore, Burlington, MA), which includes the following 23 cytokines and chemokines ...
-
bioRxiv - Biochemistry 2022Quote: ... and hearts), mouse organs (livers, brains, and hearts) and human hearts and brains was performed with a commercial kit (Sigma-Aldrich, 11814770001) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: The cytokine concentrations of organoid lysate samples were quantified on the Luminex FLEXMAP3D platform using the Milliplex human cytokine and chemokine magnetic bead panel kit (Millipore HCYTOMAG-60K), measuring a broad panel of immune signaling proteins that allow for an unbiased survey of immune cues activating a diverse set of downstream intracellular pathways ...
-
bioRxiv - Developmental Biology 2021Quote: Fish with mutations and transgenic background at 3.2 or 4 dpf were fixed 1 to 2 days at 4℃ with 4% paraformaldehyde (Sigma, P6148) in PBS ...
-
bioRxiv - Developmental Biology 2019Quote: ... Villin-CreERT2 duodenum (n=4 per genotype) were treated two days after seeding with 4-hydroxytamoxifen (4-OHT; Sigma-Aldrich) 1.25 µM for 15h ...
-
bioRxiv - Genetics 2020Quote: Exopolyphosphatase activity of wild-type PRUNE1 on medium and long chain PolyP substrates (consisting of 45, 65 and 700 orthophosphate monomers, Sigma S4379, Sigma S6253, Kerafast EUI002) was assessed by comparing to S ...
-
bioRxiv - Immunology 2023Quote: ... and methoxy forms of MA with chain lengths of 72-86 carbon atom was obtained from Sigma (M4537, Sigma-Aldrich, St. Louis, MO) [83] ...
-
bioRxiv - Biophysics 2022Quote: ... SPR2 DNA encoding residues 649-864 was generated using the polymerase chain reaction method (primers: 5’-GGCAGGACCCATATGGGCAGGAGAGGGTGGGATAATAAAGC-3’ and 5’-GCCGAGCCTGAATTCTTACTTGTCGAACTGTTGGAGATCGATTTC-3’) and individually sub-cloned into pET28 (Millipore Sigma, Burlington, MA) using engineered NdeI and EcoRI restriction endonuclease sites ...
-
bioRxiv - Neuroscience 2024Quote: ... Immunofluorescence was performed according to standard procedures with antibodies against the N-terminus of the laminin α2 chain (rat monoclonal, 4H8-2, Sigma, Saint Louis, USA), the laminin α1 chain (rat monoclonal ...
-
bioRxiv - Cancer Biology 2019Quote: ... MEFs were treated with 4-hydroxy-tamoxifen (4-OHT; 1μM, Sigma H6278). Mice were injected intraperitoneally with Tamoxifen (1 mg dissolved in corn oil ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were fixed overnight in 4% formaldehyde and 4% acrylamide (Sigma, A8887) in PBS at room temperature ...
-
bioRxiv - Bioengineering 2020Quote: ... and MEF cells fixed with 4°C 4% formaldehyde (Sigma-Aldrich, 252549) in PBS for 20 minutes at room temperature ...
-
bioRxiv - Neuroscience 2019Quote: The cells were fixed with 4% paraformaldehyde/4% sucrose(v/v)(Sigma) in PBS for 15min at room temperature (RT ...
-
bioRxiv - Developmental Biology 2020Quote: ... embryos were fixed overnight at 4°C in 4%PFA-MFSW (Millipore-Filtered Seawater buffer containing 4% paraformaldehyde) ...
-
bioRxiv - Biophysics 2019Quote: ... and fixated using 4% paraformaldehyde (PFA) (Sigma Aldrich, CAS 30525-89-4) for 1 hour after letting the cells adhere for another 30 minutes at 37 °C ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were treated with either 1uM 4-hydroxytamoxifen (4-OHT; Sigma Aldrich) dissolved in 100% ethanol or a vehicle (1:2000 100% ethanol ...
-
bioRxiv - Immunology 2020Quote: ... injection of 80 microg/g of 4-hydroxytamoxifen (4-OHT; Sigma-Aldrich) 24 hours prior to sacrifice ...
-
bioRxiv - Synthetic Biology 2019Quote: ... Anhydrotetracycline (aTc) and 4-hydroxytamoxifen (4-HT) were purchased from Sigma-Aldrich.
-
bioRxiv - Cancer Biology 2021Quote: ... 4-Phenylbutyric Acid (4-PBA) and 2DG were obtained from Sigma Aldrich.
-
bioRxiv - Neuroscience 2021Quote: ... five-month old mice were injected with 4-hydroxytamoxifen (4-HT, Sigma) dissolved in sunflower seed oil (Sigma ...
-
bioRxiv - Cancer Biology 2022Quote: ... and inflated with 4% paraformaldehyde (PFA, CAS: 30520-89-4, Sigma-Aldrich) at a pressure of 25 cm water ...
-
bioRxiv - Neuroscience 2023Quote: ... Mice were given 20 mg/kg 4-hydroxytamoxifen (4-TM) (Sigma H6278) through intraperitoneal (i.p. ...
-
bioRxiv - Neuroscience 2022Quote: ... 4-Aminopuridine (4-AP) was purchased from Sigma Aldrich (St. Louis, MO).
-
bioRxiv - Neuroscience 2022Quote: ... 4 µM 5-Fluoro-20-deoxyuridine (FDU) and 4 µM Uridine (Sigma). On day 8 ...
-
bioRxiv - Cancer Biology 2022Quote: ... tamoxifen as (Z)-4-Hydroxytamoxifen (1uM, 4-OHT; Sigma-Aldrich; Cat# H7904), and Gleevec (Imatinib mesylate ...
-
bioRxiv - Cell Biology 2023Quote: N-[4-(7-diethylamino-4-methyl-3-coumarinyl)phenyl]maleimide (CPM, Sigma) was dissolved in DMSO at 4 mg/ml as stock solution and diluted 20 times using a buffer containing 25 mM HEPES ...
-
bioRxiv - Molecular Biology 2023Quote: ... and treated with 1 μM 4-hydroxy-tamoxifen (4-OHT; Sigma-Aldrich) and 1 μM Shield1 (Clontech lab ...
-
bioRxiv - Developmental Biology 2023Quote: ... cells were treated with 1uM 4-hydroxy-Tamoxifen (4-OHT, Sigma-Alderich)