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Citations for Millipore Sigma :
1051 - 1100 of 10000+ citations for Human Tropomyosin alpha 4 chain TPM4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... standard conditions (ii) with or without Agalsidase alpha (5µl/ml) (Takeda) and (iii) with or without wortmannin (75nM) (Sigma-Aldrich). As for the fibroblasts ...
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bioRxiv - Biochemistry 2021Quote: ... standard conditions (ii) with or without recombinant enzyme Agalsidase alpha (5µl/ml) (Takeda) and (iii) with or without wortmannin (75nM) (Sigma-Aldrich). Of note ...
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bioRxiv - Biochemistry 2020Quote: ... was destarched by incubation with amylase (0.2 mL, Termamyl 120 L, alpha-amylase from Bacillus licheniformis – Type XII-A, Sigma-Aldrich) in sodium phosphate buffer (125 mL ...
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bioRxiv - Systems Biology 2020Quote: ... At time 0 medium was removed from cells and recombinant mouse tumor necrosis factor alpha (TNF-α, Sigma-Aldrich, #T7539) was added at concentrations 0-100 ng/mL as 5-minute pulse ...
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bioRxiv - Molecular Biology 2020Quote: ... ice cold methanol fixed and 4% dried milk blocked cilia pellets and deciliated cell membranes (excised from Transwell inserts) were labelled for 1 hour at room temperature with a mouse anti-alpha-tubulin antibody (T9026, Sigma) diluted 1:500 in PBST ...
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bioRxiv - Cell Biology 2021Quote: ... the imaging media with alpha factor was removed and new media with 0.1 mg/ml Pronase E (Sigma P-6911) was added to inactivate any remaining alpha factor ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were moved to a humidified chamber with primary antibody (anti-acetyl-alpha tubulin, clone 6-11B-1, Sigma, MABT868) diluted in PBX (1:250 ...
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bioRxiv - Immunology 2020Quote: ... and for ChIPseq experiments cells were subsequently fixed in PBS with 1 % paraformaldehyde (Alpha Aesar) and 10 % FBS for 10 minutes at room temperature (reaction was quenched with 125 mM Glycine (Sigma)) ...
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bioRxiv - Biochemistry 2020Quote: P-gp was incubated with a 10x molar excess of membrane scaffold protein (MSP) and a 500x molar excess of 40% L-alpha-phosphatidylcholine (PC) from soybean (Sigma) for 1 hour at room temperature with gentle agitation to facilitate formation of nanodiscs [73] ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were rehydrated through the same EtOH series and incubated at 37 °C overnight in alpha-amylase (Sigma-Aldrich A4551), 0.3 mg.mL-1 phosphate buffer 20 mM pH 7.0 ...
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bioRxiv - Genetics 2023Quote: ... membranes were incubated overnight at 4°C with primary antibodies in a 1:1 solution of TBST (TBS + 0.05% Tween20) and LiCor blocking buffer (Rabbit anti-GFP, Torrey Pines Scientific TP401; and Mouse anti-alpha-tubulin, Sigma T5168). Membranes were then washed 5x times with TBST ...
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bioRxiv - Bioengineering 2024Quote: ... The primary antibodies used included mouse anti-Integrin alpha 5/CD49e (Snaka51, Catalog Number: MABT201, Sigma-Aldrich, Dilution: 1:500), mouse anti-Integrin alpha V (L230 ...
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bioRxiv - Systems Biology 2024Quote: ... BMSCs were grown to confluence after 3 days of incubation in 48-well plates and then underwent in vitro osteoblast differentiation for 10 days with osteogenic differentiation media (alpha MEM, 10% FBS, 1% pen/strep, 1% Glutamax, 50 μg/μL ascorbic acid [Sigma, St ...
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bioRxiv - Biophysics 2020Quote: ... a solution of 20 µg ml-1 biotinylated anti Mouse IgG antibody (#115-065-003-JIR-2ml STRATECH SCIENTIFIC LIMITED) and 20 µg ml-1 anti-Dynein Intermediate chain antibody (#MAB16818 Sigma-Aldrich). Unreacted streptavidin binding sites were then blocked using biotin-BSA (#PN29130 ...
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bioRxiv - Biochemistry 2021Quote: ... 250 µg of B6 heavy and 250 µg of B6 light chain encoding plasmids were co-transfected per liter of suspended HEK293F culture using 293Free transfection reagent (Millipore Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Cells were transfected at a density of 3 x 106 with equal amounts of heavy and light chain paired plasmids using polyethyleneimine (PEI, Sigma-Aldrich) at a ratio of 1:3 of total amount of plasmid to PEI ...
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bioRxiv - Immunology 2022Quote: Monoclonal antibody production was performed using 293-6E cells (National Research Council of Canada) by co-transfection of heavy and light chain expression plasmids using 25 kDa branched polyethylenimine (Sigma-Aldrich). Culture supernatants were harvested after an incubation period of 6-7 days at 37°C and 6% CO2 under constant shaking in FreeStyle Expression Medium supplemented with penicillin (20 U/ml ...
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bioRxiv - Neuroscience 2019Quote: ... cells were blocked-permeabilized in 5% gelatin from cold water fish skin and 0.1% triton X-100 for 1 h followed by incubation with primary antibodies (mouse anti-acetylated tubulin, and rabbit anti-Neurofilament heavy chain, Sigma-Aldrich) overnight at 4 °C ...
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bioRxiv - Genetics 2019Quote: ... and incubated overnight at 4 °C with primary anti-myosin heavy chains antibody (Monoclonal Anti-Myosin skeletal, Fast, Clone My-32, Mouse Ascities Fluid, M4276, Sigma-Aldrich) diluted (1:400 ...
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bioRxiv - Biochemistry 2021Quote: ... PAR chains from a purified tetra-ADP-ribosylated H2B (1-16) peptide were subjected to treatment with Alkaline Phosphatase (Sigma-Aldrich) and Phosphodiesterase I (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: Hydrolysis of Insulin B Chain was measured via reverse phase high performance liquid chromatography (HPLC) following incubation of TLN4 (160 μg/mL) with insulin B chain (42 μM, Sigma Aldrich) in 10 mM Tris for 88 h ...
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bioRxiv - Immunology 2022Quote: ... and co-transfection of heavy and light chain expression plasmids into 293-6E cells (National Research Council of Canada) using 25 kDa branched polyethylenimine (Sigma-Aldrich). After 6-7 days at 37°C and 6% CO2 under continuous shaking in FreeStyle Expression Medium supplemented with 20 U/ml penicillin and 20 µg/ml streptomycin (all Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... the medium was supplemented with a mixture of 3 branched-chain fatty acid precursors (100 μm of 2-methyl-butyrate, isobutyrate and isovalerate, Sigma-Aldrich) or straight fatty acid precursors (100 μm of methyl-butyrate ...
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bioRxiv - Plant Biology 2022Quote: Conserved regions were amplified in a standard Polymerase Chain Reaction (PCR) using genome-specific primers (Linares et al., 1998; Liu et al., 2019) synthesized commercially (Sigma-Aldrich). These probes were labelled with digoxigenin-11-dUTP ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were seeded in biological triplicate (∼80% confluent) in HPLM plus H14 supplements and treated with 5mM of the medium-chain fatty acid OA (Sigma, #C5038) dissolved in PBS ...
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bioRxiv - Immunology 2022Quote: ... and the heavy and light chain sequences of the murine hybridomas were by obtained using the mouse Ig-primer set (69831; EMD Millipore) as described (83) ...
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bioRxiv - Genomics 2022Quote: ... Regions determined to be differential between the Illumina sequencing performed in 2017 and the PacBio sequencing performed in 2020 and 2021 were verified by polymerase chain reaction (PCR) using RedTaq (Sigma-Aldrich) according to standard protocols ...
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bioRxiv - Immunology 2023Quote: ... to block the respiratory chain via complex I and 2-deoxy-D-glucose (D8375-10MG; Sigma-Aldrich, St. Louis, MO, USA) of 10mM to inhibit glycolysis.
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bioRxiv - Cancer Biology 2023Quote: ... followed by neutralization with an equal volume of 40 mM Tris-HCl (pH 5.5).93 Multiplex polymerase chain reaction (PCR)-based genotyping was performed using REDTaq® polymerase per manufacturer recommendations (Millipore Sigma). Oligonucleotides were multiplexed as follows ...
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bioRxiv - Immunology 2024Quote: ... wells were incubated with 10 µg/mL of sera for approximately 1 hr at 20 °C then washed 4 times with PBS/T before the addition of 50 µL of 1:1000 dilution of goat anti-rabbit gamma-chain alkaline phosphatase-conjugated secondary antibody (Sigma-Aldrich) for 1 hr at 20 °C ...
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bioRxiv - Cell Biology 2024Quote: ... such as pyruvate and malate entering the chain at complex I) was measured after the addition of 5 mM pyruvate (P2256, Sigma-Aldrich) + 2 mM malate (M0875 ...
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bioRxiv - Cell Biology 2020Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Sandwich ELISA kits for quantitative detection of human Aβ 1-40 peptides in a flexible 96 well format were purchased from Millipore (cat ...
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bioRxiv - Cancer Biology 2019Quote: ... Murine mCherry-G166 GSCs glioma cells or human G166 GSCs labelled with a Red Fluorescent Cell kit PKH26 (Sigma-Aldrich) were injected into NOD SCID mice ...
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bioRxiv - Cell Biology 2020Quote: Bacterial expression vectors for human Arf1 (UniProtKB: P84077) and Arf6 (UniProtKB: P62330) were constructed using a pET-41 Ek/LIC vector kit (Novagen), as described for protein expression of human Rab-family small GTPases (Tamura and Mima ...
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bioRxiv - Immunology 2021Quote: ... and PBS-treated (n ≥ 3) control human gut xenografts and mouse jejunum were purified using GenElute Mammalian Total RNA kit (Sigma) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... EasySep™ Human NK Cell Isolation Kit) and were frozen down in CS10 CryoStor cell cryopreservation media media (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
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bioRxiv - Neuroscience 2024Quote: ... Levels of individual cytokines were determined using a Milliplex bead assay kit (Milliplex MAP human cytokine/chemokine magnetic bead panel, #MCYTOMAG-70K; Millipore) and involving the Luminex MAPIX system with data analysis using xPONENT 4.2 software (Luminex) ...
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bioRxiv - Cell Biology 2024Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Aβ and pTau levels in the organoids were quantified with Milliplex human amyloid beta and tau kit (Millipore HNABTMAG-68K) on the Luminex ...
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bioRxiv - Neuroscience 2024Quote: ... in primary neuronal lysate after Aβ or gAβ treatment from mouse or human brain were quantified by the Glycerol-3-Phosphate Assay Kit (Sigma), High Sensitivity DHAP Fluorometric Assay Kit (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: Binding of cGAS and cytoplasmic mtDNA in human and mouse brain sections was determined by a PLA kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: A 96 well ELISA plate was coated with bovine fetuin (5 μg/mL; Sigma) in carbonate buffer overnight ...
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bioRxiv - Microbiology 2019Quote: ... supernatants were collected and analyzed for total GLP-1 levels by ELISA (Millipore Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... followed by the addition of the TMB/E ELISA substrate (Merck Millipore, Billerica, MA). Absorbance was measured as described.
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bioRxiv - Neuroscience 2023Quote: ... IAPP was measured by an enzyme-linked immunosorbent assay (ELISA; Merck Millipore, EZHA-52K).
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bioRxiv - Microbiology 2024Quote: ELISA plates were coated with 1mg⍰mL of chondroitin sulphate A (CSA) (Sigma, C8529) in PBS (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... Treatment with 4-hydroxytamoxifen (4-OHT, Sigma-Aldrich cat #H7904) and Shield-1 (Cheminpharma cat #CIP-S1-0005 ...
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bioRxiv - Neuroscience 2021Quote: ... 4-Hydroxytamoxifen (4-HOT) (H-7904, Sigma, St. Louis, MO), the active metabolite of tamoxifen ...