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Citations for Millipore Sigma :
1251 - 1300 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade A KNH1144 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Puromycin-labeled proteins were identified using the mouse monoclonal antibody (1:10,000, Millipore, catalog MABE343). Protein synthesis levels were determined by analyzing total lane density from 10 kDa to 250 kDa ...
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bioRxiv - Molecular Biology 2020Quote: Premature chromosome condensation was induced using the protein phosphatase 1 inhibitor calyculin (Sigma, C5552-10UG). To determine the cell cycle stage of prematurely compacted chromosomes ...
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bioRxiv - Microbiology 2019Quote: ... PVDF membrane-bound proteins were first probed with mouse anti-HA (1:3000; Sigma, H3663) and rabbit anti-GAPDH (1:5000 ...
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: ... The proteins were then transiently transfected (pBiex-1; Escort IV, Sigma-Aldrich, St. Louis, MO) into Sf9 cells ...
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bioRxiv - Microbiology 2021Quote: ... protein extracts were incubated for 45 min with 1%v/v Benzonase Nuclease (SIGMA E1014). For the FASP digestion ...
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bioRxiv - Physiology 2021Quote: ... Japan) and anti-glial fibrillary acidic protein (GFAP) (1:2,000; mouse monoclonal, G3893, Sigma-Aldrich) were used to assess activated microglia and reactive astrocytes in ischemic regions ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was eluted in buffer B with 0.5 M KCl and 1 mM methotrexate (Sigma). Then the protein was precipitated with 40% TCA for 60 minutes on ice followed by centrifugation (10000 g ...
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bioRxiv - Biochemistry 2020Quote: N-protein was dyed by adding (3:1) Atto 488 NHS ester (Millipore Sigma 41698) to purified protein and incubating mix at 30°C for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... After that 50 µl of 1:5 dilution Protein A colloidal gold conjugate (Sigma, USA), which is used as an indicator was added to the test hole and was allowed to soak for 2 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated to 1 mg/ml using a 50,000 MWCO Amicon Concentrator (Millipore), flash frozen and stored at -80°C.
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bioRxiv - Physiology 2022Quote: Protein samples were solubilized in 1% NP-40 lysis buffer with 4% protease inhibitors (Sigma) and centrifuged for 14,000 g for 5 minutes ...
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bioRxiv - Neuroscience 2019Quote: Antibodies used were: rat anti-myelin basic protein (MBP, 1:200, MAB386, Millipore, MA, USA), rabbit anti-Olig2 (1:200 ...
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bioRxiv - Neuroscience 2019Quote: ... monoclonal anti-mouse microtubule-associated protein 2 (MAP2; Millipore Sigma, Burlington, MA; 1:1000 dilution), and monoclonal anti-mouse primary conjugated-cy3 glial fibrillary acidic protein (GFAP ...
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bioRxiv - Molecular Biology 2019Quote: RNA-renatured proteins (~40 μg) were supplemented with 1 μg anti-NF-H (N4142, Sigma), 1 μg anti-Tau (T9450 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then concentrated to 1 mg/ml using Amicon device 30K (Merck Millipore) and stored at -80°C.
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bioRxiv - Biochemistry 2021Quote: ... The protein was then concentrated to 1 mg/ml using Amicon device 100K (Merck Millipore) and stored at -80°C ...
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bioRxiv - Physiology 2020Quote: ... light chain 3B protein (LC3I/II) tubulin (1:1,000; Cat. No. T-5168; Sigma-Aldrich), or mitochondrial porin (1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... wild type and mutant proteins were treated similarly with calpain 1 (Sigma-Aldrich, Cat. #C6108) in vitro and their cleavage was assessed by immunoblotting.
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were detected using primary antibodies α-FLAG (monoclonal α-FLAG M2, Sigma, 1:1000) or α-Myc (Clone A-14 ...
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bioRxiv - Microbiology 2022Quote: ... the recombinant protein was briefly vortexed in the presence of 1% Triton X-114 (Sigma), incubated on ice for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... and stained with microtubule-associated protein 2 (MAP 2) antibody (1:500, Sigma aldrich M9942), ALK (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein levels were determined by immunoblotting using anti-FLAG clone M2 (Sigma, F3165, 1:3000).
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bioRxiv - Microbiology 2023Quote: ... Flag-hSpt16 proteins were incubated with 12.5 U of recombinant ULP-1 SUMO Protease (Sigma) for 16 h at 4°C to remove SUMO moieties.
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bioRxiv - Neuroscience 2023Quote: ... Protein expression was induced with 0.5 mM Isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich, I6758) for 2 hours at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μg of protein was incubated with 20 mM methoxypolyethylene glycol maleimide (mPEG, Sigma-Aldrich) in the presence of 3% SDS for 10 minutes at 60ºC ...
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bioRxiv - Biochemistry 2023Quote: ... concentrated protein (7-15 mg/ml) was serially diluted in 1% Genapol X-100 (Sigma). 0.3-0.5 μl of diluted protein was added to the cis (ground side ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by 1 h incubation with Protein G agarose beads (Millipore, catalog no. 16-266) at 4 °C with constant rotation ...
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bioRxiv - Microbiology 2023Quote: ... and pellets were diluted in 1× BugBuster® (10× Protein Extraction Reagent, Novagen® Merck), 50 mM NaH2PO4 pH 8 ...
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bioRxiv - Plant Biology 2023Quote: ... The Flag-tagged proteins were then detected with Flag-HRP antibody (Sigma A8592, 1:5000); Endogenous PIF3 was detected with PIF3 antibody (Agrisera AS163954 ...
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bioRxiv - Immunology 2023Quote: Recombinant SARS-CoV-2 BA.1 nucleocapsid protein was subcloned into the pET46 vector (Novagen) with an upstream 6xHis tag and expressed in Rosetta2 pLysS E ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein expression in E.coli was stimulated by IPTG (Isopropyl β-D-1-thiogalactopyranoside, Sigma-Aldrich) at 1 mM at 16°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... sativa–derived recombinant human albumin (Sigma-Aldrich), and 213μg/ml L-ascorbic acid 2-phosphate (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... 10μM recombinant human Gastrin (Sigma-Aldrich G9145), 50ng/mL recombinant human HGF (Peprotech 100-39H) ...
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bioRxiv - Cell Biology 2019Quote: Primary human osteoblasts were purchased from Sigma. Co-culture cells were titrated to determine the highest viable seeding density to allow continuous culture for the duration of the osteoclast differentiation assay ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 IU human chorionic gonadotropin (hCG, Sigma) was injected 76 h later (14:00 on day 4 of estrous cycle ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 nM human gastrin I (Sigma-Aldrich), 500 nM A 83-01 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... coated with human plasma fibronectin (Millipore Sigma) diluted to 100 μg/mL in PBS ...
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bioRxiv - Bioengineering 2019Quote: ... Human B-lymphoid (Sigma-Aldrich, Buchs Switzerland), 293T Flp-in T-REX (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... 10% human serum (Sigma, St. Louis, MO), 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Developmental Biology 2020Quote: ... and human chorionic gonadotropin (hCG) (Sigma Aldrich). Fertilized oocytes were then transferred into M2 medium (Millipore ...
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bioRxiv - Bioengineering 2019Quote: ... human tenascin-C (Millipore Sigma, Catalog #CC065), and human plasma fibronectin (Millipore Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... 10.7 μg/mL recombinant human transferrin (Sigma), and 20μg/mL recombinant human insulin (Peprotech) ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 µg/mL human transferrin (Sigma-Aldrich), 92 nM FeCl3 (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2019Quote: Recombinant human insulin (hINS) powder (Sigma Aldrich) was dissolved in 20 mM HCl (pH 2) ...
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bioRxiv - Developmental Biology 2019Quote: ... Human embryonic kidney (HEK) 293T cells (Sigma) were maintained in DMEM supplemented with 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2020Quote: Human neuroblastoma SH-SY5Y cells (Sigma-Aldrich) were grown in a 1:1 mixture of minimal essential medium (HyClone ...
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bioRxiv - Cancer Biology 2019Quote: ... and Human Adipocyte Magnetic Bead Panel (Millipore), a Bio-Plex 200 plate reader and the Bio-Plex Manager 6.1 software (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2020Quote: ... goat anti-human IgG-peroxidase (Sigma-Aldrich), and mouse monoclonal anti-NeuN (clone A60 ...
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bioRxiv - Immunology 2020Quote: ... human fibrinogen (Sigma-Aldrich, St Louis, MO); CD11b-FITC ...
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bioRxiv - Neuroscience 2019Quote: ... recombinant human TNFα (30 ng/mL, Sigma), and C1q (400 ng/mL ...