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Citations for Millipore Sigma :
1201 - 1250 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade A KNH1144 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... The virus-containing medium was then used to infect RAWs in the presence of 8 μg/mL polybrene (Sigma-Aldrich) for 24 h ...
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bioRxiv - Microbiology 2021Quote: ... The parental HEK293T cells were transduced with this virus and the HEK293T-ACE2 cell line was selected and maintained with medium containing puromycin (Sigma). ACE2 expression was confirmed by immunofluorescence staining using mouse monoclonal antibody against c-Myc antibody 9E10 (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... N2a cells’ media was exchanged with virus-containing supernatant at 48- and 72-hours post-transfection with 8µg/ml polybrene (Sigma #H9268). N2a cells were expanded and FACS sorted for mCherry+ cells.
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bioRxiv - Biochemistry 2020Quote: ... The medium was replaced after 72 h and cells containing the integrated virus were selected with Puromycin (50ng/μl) (Sigma).
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bioRxiv - Microbiology 2020Quote: ... Viruses were titrated by infecting THP-1 cells (2×105 cells/ml) with dilutions of sucrose purified virus in the presence of polybrene (8 μg/ml, Sigma) and incubating for 48 h ...
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bioRxiv - Microbiology 2020Quote: ... Free dyes and lipids were then purified away from labeled virus particles by ultracentrifugation for one hour at 4 °C at 40,000 rpm over a 6%-18% Optiprep (Sigma Aldrich) gradient ...
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bioRxiv - Molecular Biology 2021Quote: HEK293FT-rKSHV cells were generated by infecting HEK293FT cells with concentrated rKSHV.219 virus (43) in the presence of 8 µg/mL polybrene (Sigma) and by spinoculation (800 g for 30 min at room temperature) ...
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bioRxiv - Microbiology 2019Quote: ... or PMA-treated THP-1 cells (2×105 cells/ml) with dilutions of sucrose purified virus in the presence of polybrene (8 µg/ml, Sigma) for 48 h and enumerating GFP-positive cells by flow cytometry using the FACS Calibur (BD ...
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bioRxiv - Bioengineering 2022Quote: ... HeLa cells were seeded at 25% confluency in 96 well plates and transduced with virus (lentiCRISPR-v2 with or without HPRT1 guide) and 8 μg/mL polybrene (Millipore). Virus was removed the next day and 2.5 μg/mL puromycin was added to remove cells that did not receive virus two days later ...
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bioRxiv - Cell Biology 2022Quote: ... 50,000 MBS-MEFs were seeded in 6-well plates and exposed to the virus particles mentioned above in serum-free media supplemented with 6 µg/ml polybrene (Sigma). Cells with high or low expression of tdTomato were selected by FACS (BD Biosciences Aria III).
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bioRxiv - Cell Biology 2022Quote: ... Cell supernatant containing the virus was collected 3 days after transfection and 20-fold concentrated using Amicon Ultra 15 10K (Millipore) centrifugal filter ...
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bioRxiv - Cancer Biology 2022Quote: ... The same method was used for virus to be used for GSCs (DMEM was changed to NBM).The shRNA construct number TRCN0000152163 from Sigma human TRC shRNA library was used for knockdown studies of human FMOD ...
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bioRxiv - Microbiology 2020Quote: ... Mock- and virus-infected cells were harvested at 16 h post infection and stored at −80°C in RNALater (Sigma) until RNA extraction ...
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bioRxiv - Immunology 2022Quote: ... The supernatant was then passed through a 0.45 µm filter to remove debris and virus was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Sigma, UFC9100). For this ...
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bioRxiv - Cancer Biology 2019Quote: ... Functional titration was performed by transduction of cells with serially diluted virus in the presence of polybrene (4 μg/ml, Sigma) for 6 hours followed by puromycin (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... media was removed and replaced with a mixture of culture media (50%) and crude virus (50%) containing Polybrene (8 μg/ml, Sigma). Transduction media was removed and replaced with fresh culture media 24 h later ...
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bioRxiv - Neuroscience 2021Quote: ... was used to obtain 5×105 cells single-cells per well of a Matrigel-coated 6-well plate that were incubated with concentrated virus in mTeSR1 with 10 μM ROCK inhibitor and 8 μg/ml polybrene (Sigma) for 8 hours ...
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bioRxiv - Microbiology 2020Quote: ... RNA was converted to cDNA using the First Strand cDNA synthesis kit for reverse transcription (avian myeloblastosis virus [AMV]; Millipore-Sigma). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Microbiology 2019Quote: ... of 0.01 using virus diluted in virus growth media (VGM) (DMEM supplemented with penicillin/streptomycin [Corning cellgro], 25 mM HEPES [Corning cellgro], 0.3% BSA [Sigma-Aldrich]) with 0.25 μg/ml TPCK-trypsin ...
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bioRxiv - Microbiology 2021Quote: ... freeze-thaw cycles) was followed by concentration of virus suspensions (i.e., filtered lysates) using 30kDA MWCO spin concentrator (Sigma-Millipore). From the retentate ...
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bioRxiv - Immunology 2020Quote: ... 400 μl containing 300 PFU/ml of SARS-CoV-2 virus were then added to the mAb solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Influenza viruses as well as vesicular stomatitis virus strain Indiana (VSV) were propagated on Madin-Darby canine kidney (MDCKII) cells cultured in minimal essential medium (MEM-Sigma) containing 10% (v/v ...
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bioRxiv - Immunology 2020Quote: ... Virus transduction capability was then tittered on 293T-Ace2 cells treated with 50μl of 5μg/ml polybrene (Sigma-Aldritch LLC). LzGreen titer was determined by fluorescence using BZ-X700 all-in-one fluorescent microscope (Keyence) ...
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bioRxiv - Immunology 2021Quote: ... 400 μl containing 500 PFU/ml of SARS-CoV-2 virus were then added to the Ab solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting cells were resuspended at a concentration of 106 cells/mL in virus-containing medium supplemented with 10 μg/mL polybrene (Sigma), divided into 6-well plates ...
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bioRxiv - Cell Biology 2022Quote: ... mCherry-CAMSAP2 was eluted in 50 μl TEV cleavage buffer containing 0.5 μg of glutathione S-transferase-6x-histidine Tobacco etch virus protease site (Sigma-Aldrich) for 2 hr at 4 °C.
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bioRxiv - Immunology 2022Quote: ... hTert-BJ1 or U937 were infected with 2 ml of fresh virus in presence of 8µg/ml Protamine (Millipore Sigma). To generate transduced MDDCs ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was removed and the virus was resuspended in ultrapure water containing 0.005% Bovine serum albumin (Sigma-Aldrich, USA). Purified and filtrated SARS-CoV-2 pseudovirus was firstly fixed in 2% glutaraldehyde (Leagene ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were infected with either of the sgPLXNB2 clones or the control sgRNA with Cas9-GFP virus (Sigma-Aldrich #0030) at 10 IFU/cell in Opti-MEM (Thermo Fisher Scientific #31985070 ...
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bioRxiv - Molecular Biology 2023Quote: ... 75 μL of concentrated virus were added in 2 mL of medium in presence of 8 μg/mL of polybrene (Sigma) for a 60-mm plate ...
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bioRxiv - Neuroscience 2023Quote: ... The media containing lentivirus was collected and virus particles were concentrated 20- fold using Amicon Ultracel-100k (EMD Millipore 901024). For all the experiments ...
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bioRxiv - Neuroscience 2023Quote: ... Culture supernatant containing the virus was collected 3 days after transfection and 20-fold concentrated using Amicon Ultra 15 10K (Millipore) centrifugal filter ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants containing purified virus were applied onto HeLa cells for 48 h in the presence of 8 μg/mL polybrene (Sigma). Following transduction ...
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bioRxiv - Cancer Biology 2023Quote: ... 786-O cells were cultured with virus containing media and 4µg/mL polybrene (Sigma Aldrich, Cat. No. TR-1003-G) for 24 hours ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... Lentivirus transduction was performed by spinoculation at 1200 X g for 2 hours at 32° C in the presence of virus particles and 8 ug/ml polybrene (Millipore). The next day ...
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bioRxiv - Cell Biology 2023Quote: ... 600,000 cells were seeded into each well of a 6-well plate and one week later were infected with up to 100ul virus per well (and 8 ug/ml polybrene (TR-1003-G, Sigma)) overnight ...
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bioRxiv - Microbiology 2023Quote: ... Viral titres were calculated by infecting PMA-treated THP-1 cells (2×105 cells/ml) or U87 cells (105 cells/ml) with dilutions of virus in the presence of polybrene (8 μg/ml, Sigma) for 48 h and enumerating GFP-positive cells by flow cytometry using the FACS Calibur (BD) ...
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bioRxiv - Cancer Biology 2023Quote: ... The tumor cell lines and UPCs were infected with the concentrated virus in a culture media containing 8 µg/ml Polybrene (Sigma) and positively transduced cells selected by FACS for the expression of the corresponding fluorescent reporter.
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bioRxiv - Biophysics 2023Quote: ... The cells were then infected in triplicate with VSV-G-pseudotyped GFP-encoding virus in the presence of polybrene (10 µg/ml; Sigma) at room temperature for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Developmental Biology 2024Quote: ... The next day 50 µl of concentrated virus was added together with 5 µg/ml polybrene (Millipore, TR-1003-G) to the culture media ...
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bioRxiv - Immunology 2024Quote: ... Virus was plaque-purified on Vero CCL81 cells in the presence of 25 μg/mL of cytosine arabinoside (Sigma-Aldrich), and plaques in agarose plugs were amplified on Vero CCL81 cells ...
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bioRxiv - Genetics 2022Quote: ... the membrane was probed with the primary antibody overnight at 4°C (rabbit anti-beta galactosidase antibody, 15518-1-AP Protein Tech, dilution 1/1000: mouse anti-alpha-tubulin, T6199 Sigma-Aldrich, dilution 1/10 000) and then 1 hour at room temperature with the HRP linked secondary antibody (HRP-linked anti-rabbit IgG ...
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bioRxiv - Genetics 2020Quote: ... Antibody bound DNA-proteins were isolated by protein G plus/protein A agarose beads (EMD Millipore), washed ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were eluted in HBS containing 1 mM DTT and 2.5 mM desthiobiotin (Sigma-Aldrich). Proteins were spun at 100,000x g to remove aggregates before use in assays ...
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bioRxiv - Molecular Biology 2021Quote: N-protein was dyed by adding (3:1) Atto 488 NHS ester (Millipore Sigma 41698) to purified protein and incubating mix at 4°C for 1 hour with rocking ...
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bioRxiv - Microbiology 2019Quote: ... was achieved by incubating protein samples with 1 unit/ml of restriction grade thrombin (Novagen). This was carried out overnight at 4°C in 25 mM Tris/HCl ...
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bioRxiv - Genomics 2020Quote: ... Protein was precipitated from each fraction with 1/10th volume trichloroacetic acid (TCA, Sigma-Aldrich), incubated overnight at −20°C ...
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bioRxiv - Molecular Biology 2019Quote: ... protein production was induced with 0.4 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Sigma #I6758), and the culture was incubated at 37°C and 250 rpm for 4 hr ...
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bioRxiv - Biochemistry 2021Quote: ... The bacterial pellet was lysed in 2 mL 1× BugBuster™ protein extraction reagent (Novagen), incubated by slow shaking for 20 min ...