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Citations for Millipore Sigma :
1251 - 1300 of 1467 citations for African Swine Fever virus pp62 polyprotein His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... was PCR amplified from yeast constructs described above and inserted into the Nde1 and BamH1 sites downstream of sequence encoding the His6 tag and the thrombin cleavage sequence in bacterial expression vector pET28a (Novagen). IDT g-blocks® fragments were synthesized for wild-type UBE2A or UBE2B or their mutants and inserted into Nde1-BamH1-digested pET28a by sequence and ligation independent cloning (SLIC ...
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bioRxiv - Molecular Biology 2019Quote: ... The supernatant was used for FLAG-tag affinity purification with 1.2 µL of a 50% slurry of FLAG-M2 beads (Sigma-Aldrich) per 1 mL of culture ...
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bioRxiv - Cell Biology 2019Quote: ... cells were permeabilized with 0.2% Triton-X100 or 0.1% Saponin and stained with antibodies directed against the FLAG tag (M2, Sigma, 1:400), Golgin97 (CDF4 ...
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bioRxiv - Microbiology 2019Quote: The genes of either E protein or E DIII protein (residues 301-403) of ZIKV (GZ01, KU820898) without tag were cloned into pET28a vectors (Novagen) and expressed by IPTG-induction in BL21 (RIL ...
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bioRxiv - Molecular Biology 2019Quote: ... Mouse CTCF cDNA corresponding to the full-length 736 amino acids was cloned with C-terminal 6xHis tag into pFLAG-2 (Sigma). FLAG-CTCF-6xHis protein was induced in Rosseta-Gami B-cells (EMD Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... The activated beads were resuspended in 100 μL 50 mM MES (pH 5.0) and coupled with 10 μg carrier-free anti-epitope tag antibody (c-myc: Santa Cruz #sc-40; FLAG: Sigma #F1804 ...
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bioRxiv - Developmental Biology 2020Quote: ... The cDNA sequence corresponding to sim-PD (aa 361-672) was PCR amplified and cloned in-frame with a 6xHis-Tag into a modified pET-14b plasmid (Novagen). The expression plasmid was transformed into BL21 competent E ...
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bioRxiv - Biophysics 2019Quote: ... Tag cleavage was carried out overnight at 4 °C on a roller with 10 units of HRV 3C protease (Novagen/Merck chemicals Boulevard Industrial Park ...
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bioRxiv - Cancer Biology 2019Quote: HeLa was transfected with Flag-tagged Vav1 and V5-tagged VEGFR1 grown on coverslips were stained with antibody against Flag-tag (Sigma), and against V5-tag (Cell Signaling) ...
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bioRxiv - Microbiology 2021Quote: ... The hSPLUNC1 coding sequence with a 3’ sequence encoding the FLAG tag was amplified from cDNA using KOD polymerase (Sigma) and the primers (Forward ...
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bioRxiv - Biochemistry 2020Quote: ... was fused to an N-terminal TEV-cleavable His6-GCN4 tag (as described in Sosa et al. (2012)) and cloned into a pRSF-Duet1 (Merck Millipore) vector ...
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bioRxiv - Biochemistry 2021Quote: ... Purifications using 3xFlag tag were performed similarly with the following modifications: 1 ml slurry of ANTI-FLAG M2 Affinity gel (Sigma) equilibrated in flag lysis buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... the Phos-tag gels were incubated 4 × 10 min in 100 mM EDTA before electrophoretic transfer onto Nitrocellulose membrane (Sigma). Proteins were detected by immunoblotting using the following primary antibody concentrations in 5% milk (VWR)/Tris Buffered Saline -Tween20 (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... was cloned using cDNA from Arabidopsis seedlings and inserted in frame with an N-terminal His6-tag between the BamHI and EcoRI sites of the pETDuet-1 vector (Novagen). Protein expression was performed in E ...
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bioRxiv - Microbiology 2020Quote: ... All purified proteins were analyzed by SDS-PAGE followed by Coomassie blue staining as well as by Western blot using a mouse monoclonal antibody against the 6xHis-Tag (Novagen). Protein aliquots were stored at −80⁰C in 50% glycerol (v/v ...
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bioRxiv - Evolutionary Biology 2020Quote: ... brucei ALPH1 was expressed as an N-terminal truncation with a C-terminal 6xHis-Tag in RosettaBlue competent bacteria cells (Novagen) as previously described [10].
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bioRxiv - Microbiology 2021Quote: ... membranes were blocked for 1 h with 5% dried skim milk in 1X PBS containing 0.1% Tween 20 (T-PBS) and incubated with primary anti-HA epitope tag (Sigma, H6908) or anti-GFP (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mg/L avidin and 6 mM MgCl2 were added to the supernatant to improve the Strep tag binding efficiency with Strep-Tactin beads (Novagen). Next ...
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bioRxiv - Plant Biology 2021Quote: ... This was achieved using Western blotting detecting the HA-tag (antibody: Anti-HA-Peroxidase, Sigma-Aldrich, St. Louis, USA, 12013819001) and resistance against hygromycin-induced discoloration ...
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bioRxiv - Systems Biology 2020Quote: ... the cDNA was inserted in frame with a 6xHis-GST TEV-cleavable tag into a modified version of pET-30b (Novagen). The NCK2 SH3 shuffled chimeras were constructed using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... The analyzed proteins contained an SHH-tag (Sarkari et al., 2014) and were detected using anti-HA (Sigma-Aldrich, USA) antibodies and anti-mouse IgG-HRP (Promega ...
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bioRxiv - Microbiology 2020Quote: Monoclonal antibody against FLAG tag (ANTI-FLAG M2) and β-Actin were purchased from Sigma (Cat.No. F1804 and A2228, respectively). Monoclonal antibody against human IFITM1 (Cat.No ...
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bioRxiv - Biophysics 2021Quote: ... The GB1 tag was cleaved from the oxidized proteins by incubating overnight with thrombin (10 U.mg−1 protein, Sigma-Aldrich). The cleaved protein was purified by reversed phase high-performance liquid chromatography (RP-HPLC ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein pellet was resuspended in digest buffer (0.1 M triethylammonium bicarbonate, pH 8.5, Sigma-Aldrich, tandem mass tag (TMT) labeling using a 6-plex TMT kit (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... in this work referred to as Pif1) fused at its N-terminus to a 6-histidine tag was cloned into vector pET28 (Novagen) and transformed into E ...
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bioRxiv - Microbiology 2022Quote: ... and incubated overnight at 4°C with specific primary monoclonal (MAbs) or polyclonal (pAbs) antibodies: anti-HA tag (rabbit pAb; H6908, Sigma), anti-GFP (rabbit pAb ...
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bioRxiv - Biochemistry 2020Quote: ... and a His8 tag was ligated into the corresponding sites in the first multiple cloning site of pCDFDuet-1 (Novagen), yielding plasmid pRHJ001 ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were blocked with 5% skimmed milk in Tris Buffered saline-Tween for 1 h before incubation with primary antibody anti-myc tag monoclonal clone 4A6 (Millipore) diluted to final concentration of 200 ng mL−1 in 3% skimmed milk in TBST for 1h at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Removal of the GST-tag from human and zebrafish Vps29 was performed using on-column cleavage with thrombin (Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Bound scFv were detected using murine mAb 9E10 which recognizes the C-terminal c-myc tag (1:50 diluted in 2% MPBST) and a goat anti-mouse serum conjugated with horseradish peroxidase (HRP) (A0168, Sigma) (1:42000 dilution in 2% MPBST) ...
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bioRxiv - Biochemistry 2020Quote: ... PCNA-12 co-expression plasmids were constructed by inserting non-tagged PCNA-1 into multiple cloning site 1 and PCNA-2 (without and with a His6-tag) into site 2 of pET-DUET-1 plasmid (Novagen).
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bioRxiv - Microbiology 2022Quote: Recombinant 6XHis-tag GreA or 6XHis-tag GreB were produced by cloning greA and greB genes into NdeI and BamHI sites of the pET14B vector (Novagen) using greA F and greA R or greB F and greB R primers ...
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bioRxiv - Microbiology 2022Quote: ... Anti-FLAG tag or anti-mCherry primary antibodies were used at a concentration of 1:1,250 (Sigma, St. Louis, MO). Then ...
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bioRxiv - Cell Biology 2019Quote: ... carrying two isoleucine residues at the N-terminus and a hexahistidine tag at the C-terminus was expressed in Escherichia coli Rosetta2(DE3)pLysS cells (Novagen) and purified by immobilized metal-affinity chromatography followed by gel-filtration chromatography as described previously (Bishop et al. ...
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bioRxiv - Microbiology 2019Quote: ... This fragment was cloned by ligation independent cloning into the linearized pIEX-10 EK-LIC vector which carries a C-terminal 10xHis tag (Novagen) to generate pIEX-10 ...
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bioRxiv - Molecular Biology 2021Quote: MBL protein levels were determined by SDS-PAGE followed by western blotting with Strep-tag II monoclonal antibodies (at a 1:1000 dilution from 200 μg/ml solution) (Novagen) and immunoglobulin G-alkaline phosphatase conjugates (at a 1:3000 dilution) ...
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bioRxiv - Bioengineering 2019Quote: For Flag-tag detection membranes were incubated for two hours with the monoclonal anti-Flag M2 antibody from mouse (F3165, Sigma) in a 1:5000 dilution (in TBS-T ...
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bioRxiv - Cell Biology 2021Quote: A Rab11/Ypt32 construct harbouring a GST tag at the N-terminus was expressed recombinantly in Rosetta2 cells (EMD Millipore) and purified using the procedure described previously (21) ...
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bioRxiv - Physiology 2020Quote: ... The presence of LPL in the conditioned media was confirmed through Western blotting using a mouse antibody against the FLAG tag (1:5000; Sigma). LPL activity was assessed through an LPL activity assay (see below).
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bioRxiv - Developmental Biology 2020Quote: Coding regions for all protein fragments used for EMSA were cloned in-frame with an N-terminal 6xHis-tag in the pET14b vector (Novagen) and expressed in BL21 cells ...
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bioRxiv - Microbiology 2022Quote: ... the soluble portion of these proteins (excluding the leader peptide and the predicted α1N helix) was fused to non-cleavable N-terminal 6His or Strep II tags and cloned into pET-28b (Novagen). We amplified pilA directly from the S ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tag-free Mpro in the flow-through was concentrated by using Amicon Ultra 15 centrifugal filters (10 kD, Merck Millipore) at 2773 x g ...
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bioRxiv - Microbiology 2022Quote: ... for 5 minutes and then migrated on a 10% SDS polyacrylamide gel and analyzed by Western blot analysis using a monoclonal antibody specific for the FLAG tag (Sigma). Proteins were detected using a goat anti-mouse IgG coupled to IRDye 800CW and LI-COR analysis.
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bioRxiv - Immunology 2022Quote: ... Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was performed using an Apop Tag Plus Peroxidase In Situ Apoptosis Kit (Merck Millipore) following the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2022Quote: ... The proteins were separated by 10 % SDS-PAGE gels and detected by immunoblotting with the first anti-GFP or HA-tag antibodies (Sigma) and the second anti-rabbit IgG-peroxidase antibody (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmids used to produce ActRII and ActRIIB in bacteria were constructed by inserting the corresponding coding sequence downstream of the N-terminal His6 tag and thrombin cleavage site in pET15b (EMD-Millipore). The plasmid used to produce BMPRII extracellular domain in mammalian cells was constructed by inserting the coding sequence for this downstream of the rat serum albumin signal peptide ...
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bioRxiv - Cell Biology 2024Quote: Antibodies that were used in this study to detect viral and cellular proteins were the monoclonal antibody (MAb) recognizing the FLAG-tag epitope anti-Flag (clone M2, Sigma), the MAb against HCMV pp28 ...
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bioRxiv - Microbiology 2022Quote: ... NSm and the entire pcDNA3.1 vector encoding Gn with an N-terminal SS plus HA tag were amplified by PCR using KOD Hot Start DNA polymerase (Novagen), mixed and then concatenated using the NEBuilder HiFi assembly kit according to the manufacturer’s instructions to yield pSnH-OROV-M ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins tagged with the TAP (tandem affinity purification) or HA (hemagglutinin) tag were detected with PAP or an anti-HA antibody (Sigma, Taufkirchen ...
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bioRxiv - Genetics 2023Quote: ... 0.1% Tween-20 and 5% non-fat milk for 1 hour at room temperature and probed for 18 hours at 4 °C with antibodies specific for Flag tag (M2, SIGMA), Myc tag (9E10 ...