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Citations for Millipore Sigma :
1251 - 1300 of 10000+ citations for 3 3 17 17 Bis ethylenedioxy 19 hydroxyandrost 5 ene 19 d2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... Samples were washed (3 times, 5 min each) with PBS containing 0.1% Triton X100 (Sigma-Aldrich), and then incubated with secondary antibody (Table 1 ...
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bioRxiv - Cell Biology 2024Quote: ... human Spindly siRNA (Sequence antisense: 5’-GAAAGGGUCUCAAACUGAA-3’, dTdT overhangs, Sigma-Aldrich, St. Louis, MO, USA), and control siRNA (D-001810-10-05 ...
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bioRxiv - Physiology 2024Quote: ... tissues were washed (3 x 5 min) in PBS containing 0.1% Triton X-100 (Sigma-Aldrich) and incubated with mouse anti-TARDBP primary antibody or mouse anti-HuC/D (for total neurons ...
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bioRxiv - Molecular Biology 2024Quote: ... 3-(4,5-dimethylthiazolyl-2-yl)-2–5 diphenyl tetrazolium bromide (MTT, Sigma, St. Louis, Missouri, USA) was introduced ...
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bioRxiv - Cell Biology 2024Quote: ... The control siRNA is a scrambled sequence (5’-UUCUCCGAACGUGUCACGUdTdT-3’) (NC, Sigma, St. Louis, MO, USA). A total of 60 nM siRNAs was used for transfection using Lipofectamine-RNAi-MAX Reagent (13778 ...
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bioRxiv - Bioengineering 2024Quote: ... KG) followed by incubation in freshly made 5% (3-Aminopropyl) triethoxysilane (Sigma-Aldrich, Cat. no. 440140) in deionized water for 20 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... Staining was detected with 5-bromo-4-chloro-3’-indolylphosphate/nitro-blue-tetrazolium (BCIP/NBT, Sigma).
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bioRxiv - Molecular Biology 2020Quote: ... The pellets were resuspended at an OD600 of 1 in 5 ml of agroinfiltration buffer (10 mM MgCl2 and 250 μM of 3’,5’-Dimethoxy-4’-hydroxyacetophenone (Sigma-Aldrich)) ...
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bioRxiv - Neuroscience 2020Quote: ... flies were collected 2-5 days post eclosion and grown for another 3-5 days on 1mM all-trans retinal (R2500; Sigma-Aldrich) supplemented food in complete darkness before experimental testing was performed ...
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bioRxiv - Neuroscience 2020Quote: ... were performed in DJ-1-deficient cells with MTT (3-(4, 5-dimethylthiazol-2-yl)−2–5-diphenyltetrazolium bromide) (Sigma-Aldrich) as described in [24] ...
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bioRxiv - Bioengineering 2022Quote: ... The cells were then incubated in a blocking buffer (5% normal goat serum, Vector; 5% normal donkey serum, Wako; 3% BSA, Sigma-Aldrich; and 0.1% Tween-20 ...
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bioRxiv - Immunology 2020Quote: ... Mice were subjected to cutaneous immune challenge by applying 20 µl of 3-5% 4- Ethoxymethylene-2-phenyl-2-oxazolin-5-one (Sigma-Aldrich) in 1:2 acetone:olive oil ...
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bioRxiv - Biophysics 2024Quote: Adenosine 3’,5’-cyclic monophosphate sodium salt monohydrate (cAMP) and guanosine 3’,5’-cyclic monophosphate sodium salt (cGMP) were purchased from Sigma Aldrich and both prepared at 16 mM in 150 mM KCl ...
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bioRxiv - Microbiology 2021Quote: ... The GC-FID system was calibrated for hop-22(29)-ene (Sigma Aldrich, 0.1 mg mL−1 in isooctane) using a 6-point calibration ...
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bioRxiv - Microbiology 2021Quote: ... 5α-cholestane (≥ 97.0%) and hop-22(29)-ene (0.1 mg/mL in isooctane, analytical standard) were obtained from Sigma-Aldrich. Other reagents and solvents were purchased in HPLC-grade or pro-analysis quality from Sigma-Aldrich and all consumables were from VWR (Ismaning ...
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bioRxiv - Biochemistry 2022Quote: All photo-induced thiol-ene reactions were conducted in an Aldrich® AtmosBag (Sigma-Aldrich, Saint Louis, MO, USA) filled with nitrogen gas ...
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bioRxiv - Developmental Biology 2022Quote: ... 3-dpf larvae were anesthetized with 0.016 % Tricaine/Ethyl 3-aminobenzoate methanesulfonate salt (A5040, SIGMA-ALDRICH) and microinjected with 5-8 nL of liposome encapsulated clodronate (SKU# CLD-8909 ...
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bioRxiv - Bioengineering 2021Quote: ... scaffolds were rehydrated in PBS overnight with 1-ethyl-3-(3- dimethylaminopropyl) carbodiimide (EDAC, Sigma-Aldrich) and N- hydroxysuccinimide (NHS ...
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bioRxiv - Plant Biology 2021Quote: ... Roots samples were vacuum infiltrated with 3-3’-diaminobenzidine tetrahydrochloride (1.25 mg/mL, DAB, Sigma-Aldrich), Tween-20 (0.05% v/v ...
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bioRxiv - Plant Biology 2021Quote: ... The RNA was fixed by using 1-ethyl-3-[3- dimethylaminopropyl] carbodiimide hydrochloride (EDC, Sigma-Aldrich) for 1 h at 60°C ...
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bioRxiv - Pathology 2024Quote: ... concentrated 10 times using Amicon Ultra-4 Ultracel-3 3 kDa centrifugal filter units (Millipore, UFC800308). Aliquots of concentrated media and flowthrough media were frozen at −80°C.
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bioRxiv - Pathology 2024Quote: ... concentrated 10 times using Amicon Ultra-4 Ultracel-3 3 kDa centrifugal filter units (Millipore, UFC800308). Aliquots of concentrated medium and flowthrough medium were frozen at −80°C.
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bioRxiv - Cell Biology 2023Quote: ... sections were washed 3 times with 0.01% PBS-T and DAPI (Sigma-Aldrich, #28718-90-3) nuclear stain ...
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Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: ... 3’-3’ linkage of CpG with some staples were applied by dibenzocyclooctyne (DBCO)-azide (Sigma, US) click chemistry (Fig ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were cultured in medium supplemented with 2 mM 3-phosphoglycerate (3-PG, Sigma-Aldrich, #P8877) or 2 mM serine (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... blocked for one hour in blocking buffer (3% goat serum and 3% BSA in PBS (Sigma)) and incubated with pneumococcal antisera Pool Q (SSI Diagnostica ...
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bioRxiv - Synthetic Biology 2024Quote: ... cat # K3007) and 3-O-C12-HSL (N-(3-Oxododecanoyl)-L-homoserine lactone from Sigma-Aldrich, cat # O9139 ...
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bioRxiv - Developmental Biology 2024Quote: ... allowed to dry and incubated for 3 min with (3-aminopropyl)trimethoxysilane (APTMS, Sigma Aldrich, 281778). APTMS was then washed off and the glass bottoms were incubated with 0.5% glutaraldehyde for 30 min ...
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bioRxiv - Genomics 2020Quote: ... incubated with primary antibodies (anti-AP-1 Thermo Scientific™ #MA5-15172, anti-Sp1 ChIPAb+™ Merck #17-601, anti-FLAG Sigma Aldrich #F3165 ...
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bioRxiv - Microbiology 2020Quote: ... were immunized intraperitoneally with 17 μg of recombinant NRP1 b1b2 mixed with an equal volume of complete Freund’s adjuvant (Sigma–Aldrich Chemie, Steinheim, Germany), followed by a booster immunization four weeks later of the same dose mixed with incomplete Freund’s adjuvant (Sigma–Aldrich) ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: Cell culture supernatant was diluted 1:1 in binding buffer (0.16 M sodium phosphate, 500 mM NaCl, pH 9) and loaded on a Protein A Sepharose (Sigma Aldrich, 17-1279-03) column at a flow rate of 2 ml/min ...
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bioRxiv - Cell Biology 2020Quote: ... The sheared chromatin was then incubated with anti-ARID1A antibody and purified by using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit (Millipore, 17-10086). Chromatin DNAs quality was assessed by 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... and 78.1 µL of a 10 mg/mL (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl N-succinimidyl carbonate (BCN, 17 eq.) (Sigma Aldrich, Cat. No. 744867-10MG) and stirred at 4°C for 16 h ...
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bioRxiv - Developmental Biology 2020Quote: ... These EpiSC derived OPCs were sorted to purity by fluorescence activated cell sorting using conjugated CD140a-APC (eBioscience, 17-1401; 1:80) and NG2-AF488 (Millipore, AB5320A4; 1:100) antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... untagged human α-synuclein (100 μg/well) —purified as previously described [17]—and 10 μM ThT in dPBS (Sigma-Aldrich, St. Louis, MO) were combined in a final volume of 95 μl of dPBS ...
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bioRxiv - Immunology 2020Quote: ... Single-cell suspensions of caecal tonsils from vaccinated and non-vaccinated birds at 17 and 21 dph were concentrated for lymphocytes by density centrifugation over Histopaque (1.077 g/mL; Sigma–Aldrich, St. Louis, MO). For CD4+/CD8+ T-cell ratio analysis ...
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bioRxiv - Developmental Biology 2021Quote: 15 embryos at HH11 were treated following the manufacturer’s recommendations (Milipore, Magna ChIP A#17-610) with formaldehyde (Sigma, formaldehyde solution 37% #F1635) and Glycine (Merck #5.00190.1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... these EpiSC derived OPCs were purified by fluorescence activated cell sorting using conjugated CD140a (eBioscience, 17-1401; 1:80) and NG2-AF488 (Millipore, AB5320A4; 1:100) antibodies ...
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bioRxiv - Immunology 2022Quote: Cells were isolated from human blood using density gradient centrifugation agent Ficoll®-Paque Premium (17-5442-02, GE Healthcare, SIGMA, Darmstadt, Germany). Monocytes were plated at 3×105 cells per well in plastic 24 well plates ...
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bioRxiv - Immunology 2023Quote: Cells from day 5 bone marrow cultures from tet-LIN28B or littermate control mice were used for lysis and RIP using Magna RIP RNA-Binding Protein Immunoprecipitation kit (Millipore, cat#17-700) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... HCF were seeded at a density of 17 500 cells/cm2 in fibronectin-coated (#F0895, Sigma Aldrich, 0.1% solution; 1:1000 in water) 12-well plates and 8-well μ-Slides for indirect co-culture ...
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bioRxiv - Genomics 2022Quote: ChIP-seq was performed as previously described[17] by using the following antibodies (2 μg per reaction) for: H3K4me3 (Sigma-Aldrich, 07-473), H3K36me3 (Diagenode ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 3x PBS washes for 5 minutes at RT before blocking with blocking buffer (BB; 5% normal donkey serum Sigma-Aldrich #S30-M, 3% BSA (Sigma #A4503) and 0.25% Triton-X100 (Sigma #T9284 ...
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bioRxiv - Cell Biology 2020Quote: ... 3% β-mercaptoethanol (#M6250, Sigma, Austria), 0,01% bromophenol blue (#44305 ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μg/ml of nocodazole (Sigma) in DMSO was added to the cells ...
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bioRxiv - Cell Biology 2020Quote: ... and phosphatase inhibitor cocktail 3 (Sigma) prior to immunoprecipitation with FLAG agarose beads (Sigma).
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bioRxiv - Neuroscience 2020Quote: ... 3-dione (CNQX) (Sigma, C127, USA), GABA (Sigma ...
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bioRxiv - Immunology 2021Quote: ... epigallocatechin-3-gallate (EGCG) (Sigma Aldrich) was used at 10 μM or 20 μM ...
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bioRxiv - Genetics 2021Quote: ... 3- indoleacetic acid (IAA) (Sigma, I2886) was dissolved in NaOH 1N and used at a final concentration of 0.5 mM ...
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bioRxiv - Developmental Biology 2021Quote: ... and eosin (Sigma HT110-2-3) for two minutes each ...