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Citations for Millipore Sigma :
1201 - 1250 of 10000+ citations for ERK1 2 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... recombinant FLAG-tagged zebrafish Grk1a and Grk1b were each inserted into the pFLAG-CMV2 vector (#E7033, Millipore-Sigma). HEK-293 cells cultured in DME/F-12 with 10% fetal bovine serum were transfected with either of these constructs using FuGENE 6 Transfection Reagent (#E2691 ...
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bioRxiv - Bioengineering 2022Quote: ... Epidermal growth factor (EGF) and Recombinant Human Fibroblast Growth Factor Basic (FGF2) Progesterone were purchased from Sigma-Aldrich. OPSS-PEG-SVA (3400 Da) ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant FLAG-tagged zebrafish Grk1a and Grk1b were each inserted into the pFLAG-CMV2 vector (#E7033, Millipore-Sigma). HEK-293 cells cultured in DME/F-12 with 10% fetal bovine serum were transfected with either of these constructs using FuGENE 6 Transfection Reagent (#E2691 ...
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bioRxiv - Biochemistry 2019Quote: ... The pure recombinant protein was eluted from the column using the same buffer with 0.1 mg.mL-1 FLAG peptide (Sigma). Pure PRMT7 was flash frozen and stored at −80 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... The concentration of total protein in CFE with recombinant Scaf19LKT was determined by Bradford reagent (Sigma-Aldrich, USA), CFE was diluted 1,000-times in 0.1 M sodium carbonate coating buffer (pH 9.0) ...
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Cell Biology 2020Quote: ... the cell medium was replaced with DMEM containing 0.2% FBS and 300ng/mL IGF-1 (human recombinant, Sigma) for 3 hours ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2.5μg/ml of recombinant RBD protein in carbonate-bicarbonate coating buffer (Sigma). After blocking with PBS containing 1% BSA ...
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bioRxiv - Biochemistry 2022Quote: ... and ∼1 μg recombinant GST or GST-SMN in freshly made 25 mM bis tris propane (Sigma B6755) pH 6.8 buffer with 1 M NaCl and 0.05% NP-40 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells that were cultured in 0.5 % FBS were stimulated with hEGF (recombinant EGF) (Sigma Alrdich, Catalog No: E9644) in concentrations (10 and 30 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... except for the control group for which recombinant fusion proteins were mixed with incomplete Freund adjuvant (IFA, Sigma). Blood samples were collected from the submandibular vein at different time points for more than 6 months and sera were prepared and analysed for the presence of specific antibodies by ELISA as described below ...
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bioRxiv - Immunology 2022Quote: ... a control group received injection of recombinant fusion proteins (3.8 μM) mixed with complete Freund adjuvant (CFA, Sigma) in 50 μl of PBS ...
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bioRxiv - Microbiology 2022Quote: ... Assay was conducted according to the manufacturer’s instructions and recombinant L-Lactic Dehydrogenase from porcine heart (Sigma-Aldrich) was used to generate a standard curve ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.1 IU/mL insulin and 10 ng/mL human recombinant epidermal growth factor (EGF) (all from Sigma-Aldrich). TKCC10 cells were grown in a 1:1 mixture of M199 medium and Ham’s F12 medium ...
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bioRxiv - Biochemistry 2023Quote: ... cells were incubated with medium containing 10 µg/mL recombinant human adiponectin (SRP4901, Sigma-Aldrich, in sterile water), a quantity equivalent to typical adiponectin levels in lean women (Ding et al. ...
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bioRxiv - Immunology 2023Quote: RVT1 association with the recombinant form of human PF4 and UPS-grade protamine (Millipore-Sigma, St. Louis, MO) were characterized using a Synapt G2 HDMS (Waters Corp. ...
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bioRxiv - Immunology 2023Quote: RVT1 association with the recombinant form of human PF4 and UPS-grade protamine (Millipore-Sigma, St. Louis, MO) were characterized using a Synapt G2 HDMS (Waters Corp. ...
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bioRxiv - Bioengineering 2023Quote: ... mESC maintenance media was supplemented with 1,000 units/mL of Recombinant Mouse Leukemia Inhibitory Factor (Millipore Sigma # ESG1107). Media was changed daily.
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were purified using a Ni-NTA column (Yeasen, Wuhan, China) and concentrated by ultrafiltration (Millipore). Protein concentration was determined by SDS-PAGE using BSA as a standard.
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bioRxiv - Biochemistry 2023Quote: ... and the recombinant protein was eluted using buffer D supplemented with 10 mM reduced L-glutathione (Sigma–Aldrich). The purified protein was digested with TEV protease overnight ...
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bioRxiv - Biochemistry 2023Quote: His-PP-Pex6 1-204 protein (purified by recombinant expression from plasmid pDC69) was digested with 0.1 or 0.2 μg/μL trypsin protease (Sigma) for 10 minutes at 23 °C in 100 mM Tris pH 8.0 buffer ...
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bioRxiv - Cell Biology 2023Quote: ... either coated with BSA or recombinant Laminin332 (BioLamina LN332-0502, diluted in Dulbecco’s Phosphate Buffered Saline, Sigma, D8662) and left to adhere overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... 250-500 μg of recombinant proteins were resuspended in HAT buffer supplemented with 0.75 mM acetyl CoA (Sigma) and 1 μl butyric acid (200 mM ...
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bioRxiv - Immunology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were blocked with 5% milk in Tris buffered saline (TBS) and probed with antibodies to HCMV IE1 and IE2 (Merck Millipore; MAB-8131; 1/5000), pp52 (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2022Quote: ... and −7173 of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2023Quote: ... of anti-spike murine antibodies (including cross-reactive mAbs against SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Neuroscience 2023Quote: ... we added both the XPoSE-tag and neuronal nuclear marker NeuN (anti-NeuN Mouse mAb clone A60, MAB377X, Millipore Sigma, RRID: AB_2149209, 1:500) We incubated the samples with antibody for 15 minutes at 4 °C on a rotating mixer ...
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bioRxiv - Microbiology 2023Quote: ... and -71)60 of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Bioengineering 2024Quote: ... Washes were followed by the addition of 100 μL of anti-FLAG horseradish peroxidase (HRP)-linked monoclonal antibody (mAb, #A8592, Sigma Aldrich, St. Louis, MO) for 1 h at room temperature with rocking ...
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bioRxiv - Microbiology 2024Quote: ... and -71 60 of anti-spike murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Cancer Biology 2021Quote: ... Human interleukin-2 (IL-2) (H7041) was purchased from Sigma Aldrich. Ficoll-PaqueTM PLUS (17-1440-02 ...
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bioRxiv - Neuroscience 2020Quote: ... in 5 mL of 2-methyl-2-butanol (Sigma Aldrich, #240486). Mice were deeply anesthetized using 15 µL of Avertin 2.5% per gram of body weight ...
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bioRxiv - Bioengineering 2021Quote: ... (2) hexafluorophosphate azabenzotriazole tetramethyl uronium (HATU; 2 equiv:ELP amine, Sigma, 445460), and (3 ...
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bioRxiv - Neuroscience 2021Quote: ... 2 mM penicillin/streptomycin and 2 mM L-glutamine (Sigma, UK). Hek-293T cells were plated in 24 well plates for 24 hours before transduction with LV-LTα or LV-GFP at MOI 5 ...
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bioRxiv - Biochemistry 2022Quote: ... 2 μM ponatinib (Selleckchem) or 2 μM iodoacetamide (IAA) (Sigma-Aldrich), the other half was treated with vehicle (DMSO or water ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μg/mL 4′,6-Diamidin-2-phenylindol (DAPI, Sigma-Aldrich) in 1X PBS was added for 2 min followed by a final washing step ...
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bioRxiv - Microbiology 2020Quote: ... 2% glycerol or 2% casamino acids and in RPMI-1640 (Sigma, containing L-glutamine ...
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bioRxiv - Microbiology 2022Quote: ... E2 and P4 were solubilized in 2-butanol (2-BtOH; Sigma) as our previous studies demonstrate that this reagent does not interfere with NgPLD activity ...
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bioRxiv - Biochemistry 2019Quote: 2 µl of 1 mM 2’,7’ Dichlorofluorescein diacetate (Sigma-Aldrich) dissolved in DMSO was added to 198 µl of cell culture in a black f-bottom 96 well plate (Greiner bio-one ...
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bioRxiv - Biochemistry 2019Quote: ... Released glycans were labelled with 2-aminobenzamide (2-AB; Sigma-Aldrich) and purified on Whatman 3 mm chromatography paper ...
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bioRxiv - Bioengineering 2020Quote: ... The photoinitiator (2-hydroxy-2-methylpropiophenone, Darocur 1173, 405655, Sigma-Aldrich) is introduced with the two precursors.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 25mM Hepes (N-[2-hydroxyethyl]piperazine-N0-[2-ethanesulfonic acid; Sigma]), supplemented with 10% heat inactivated FBS (GIBCO ...
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bioRxiv - Molecular Biology 2019Quote: ... or MG132 (10 μM, 2 h, replicates 2, 3, 4; Sigma). Proteins were precipitated in acetone ...
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bioRxiv - Immunology 2020Quote: 2-deoxy-d-glucose (2-DG; Sigma-Aldrich, St. Louis, MO) treatment of wounded zebrafish larvae (simple tail transection ...
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bioRxiv - Neuroscience 2022Quote: 2-Bromopalmitate (or 2-bromohexadecanoic acid, Sigma-Aldrich, Cat no. 238422) was dissolved in dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2022Quote: ... 2% pectone (Difco) and 2% glucose (Sigma-Aldrich, Carlsbad, CA, USA) or selective medium YNB containing ...
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bioRxiv - Synthetic Biology 2022Quote: ... was mixed with photo-initiator 2-hydroxy-2-methylpropio-phenone (Sigma) (90/10% w/w) ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µg/ml doxycycline and 2 µM Ara-C (Sigma-Aldrich). From day 3 – 6 ...