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Citations for Millipore Sigma :
1001 - 1050 of 10000+ citations for ERK1 2 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... recombinant PRDX6 was purified by gel filtration chromatography on a Superdex 200 16/600 column (Sigma) equilibrated with 20mM HEPES at pH 7.0 ...
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bioRxiv - Microbiology 2020Quote: ... were treated with or without 25-100 U/mL of human recombinant IFN-α (Sigma-Aldrich) for 4-12 hours after which total RNA was isolated and subjected to qRT-PCR in order to measure expression of ISGs.
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bioRxiv - Neuroscience 2022Quote: Fixation was performed following 24 hrs of recombinant protein treatment using 4 % PFA / 4% Sucrose (Sigma) in 1x PBS at RT for 15 min ...
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bioRxiv - Microbiology 2019Quote: ... EVs were incubated with Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) at different final concentration (0 to 10 µg/ml) ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant protein was concentrated by centrifugation in Amicon® Ultra-15-30K (Millipore Sigma, Burlington, MA), purified on protein A/G columns (NAb™ Spin Kit ...
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bioRxiv - Microbiology 2021Quote: For expression of C13/N15 labeled recombinant human wildtype-MIF BL21-(DE3)-Rosetta-pLysS bacteria (Novagen) were transformed with pET11a-MIF and spread on LB-Agar plates containing 50 µg/mL carbenicillin and 34 µg/mL chloramphenicol ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 µg/ml BSA fraction V and 10 µg/ml human recombinant in sulin (both Sigma)) ...
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bioRxiv - Cell Biology 2021Quote: ... The recombinant hormone was dissolved in sterile Lebovitz medium (L-15) (Sigma-Aldrich, St. Louis, USA) at the concentration of 100 µg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human EGF (Cat# E9644) and Glucose Quantification Kit (Cat# GAGO-20) purchased from Sigma-Aldrich; Cisplatin (Cat# 232120 ...
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bioRxiv - Microbiology 2020Quote: ... hAEC cultures were pretreated with recombinant universal type I IFN (100 or 10 IU/ml; Sigma) or recombinant IFN-λ3 (100 or 10 ng/ml 56 ...
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bioRxiv - Bioengineering 2022Quote: ... Beefy-9 medium was prepared by adding 0.8 mg/mL of recombinant albumin (Sigma #A9731-1G) to Hi-Def B8 ...
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bioRxiv - Molecular Biology 2022Quote: ... The recombinant HNRNPH1 was analyzed using an anti-FLAG antibody (F1804, Sigma-Aldrich, St. Louis, MO) and an anti-mouse HRP secondary antibody (Cell Signaling Technology ...
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bioRxiv - Biochemistry 2024Quote: Recombinant proteins to be labeled with fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼100 μM ...
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bioRxiv - Molecular Biology 2023Quote: ... TGFB1 signaling was induced with 5 ng/ml final concentration of human recombinant TGFB1 (Sigma-Aldrich) for 4 h.
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bioRxiv - Cell Biology 2023Quote: ... cells were cultured with recombinant retroviruses or lentiviruses and 10 μg/mL polybrene (H9268; Sigma-Aldrich), and they were selected in growth medium with puromycin (14861-71 ...
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bioRxiv - Pathology 2023Quote: ... 10 µL of recombinant Tat-Cre at 8.8 µg/µL supplemented with Fast Green FCF (Sigma) to facilitate visualization of TAT-Cre delivery were intravaginally injected to each uterine horn using a micropipette tip (Figure 4A) ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35±3 days25 ...
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bioRxiv - Physiology 2023Quote: ... and recombinant human interleukin-1β (IL-β) were obtained from Millipore Sigma (St Louis, MO, USA). Thapsigargin (TG ...
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bioRxiv - Immunology 2024Quote: ... Recombinant lyophilized proteins were resuspended using 0.1% (w/v) human serum albumin (HSA) (A9731, Sigma-Aldrich) in sterile water ...
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bioRxiv - Cell Biology 2024Quote: ... Fractions containing recombinant protein were pooled and concentrated on a 30K Amicon Ultra Centrifugal filter (Millipore). Glycerol at 10% final was added to the sample and protein concentration was estimated using Bradford detergent compatible assay (Pierce) ...
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bioRxiv - Immunology 2024Quote: ... 10 μg/mL dsDNA or 10 μg/mL recombinant human insulin (Sigma Aldrich, St. Louis, MO). After five washes in PBS with 0.05% Tween 20 ...
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bioRxiv - Immunology 2024Quote: ... 20 ng/mL recombinant mouse GM-CSF (Miltenyi-Biotec) and 0.5 μM ß-estradiol (Sigma-Aldrich) and transduced with ER-HoxB8 retrovirus using Lentiblast Premium (OZ Biosciences).71 After two weeks ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mg/mL rabbit anti-Myc (EMD Millipore, 06-549 [rabbit]), 1:500 mouse anti-V5 (Thermo Fisher ...
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bioRxiv - Developmental Biology 2024Quote: ... 1uL Rabbit anti-Ham or 1ul Rabbit IgG (SIGMA-ALDRICH, I5006) was added for an overnight incubation at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-RHEB (Fisher #PIPA520129) and rabbit anti-TSC2 (Sigma #HPA030409). After washing three times in 0.1M PBS ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was washed in TBST 2 × 15 min and incubated for 1 hour in room temperature with HRP-conjugated anti-rabbit antibody (Sigma-Aldrich, NA9043, 1:2,500) diluted in TBST with 5 % non-fat dry milk ...
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bioRxiv - Neuroscience 2021Quote: ... The slices were rinsed with the blocking solution and incubated with secondary antibodies for 2 h at room temperature (FITC-conjugated anti-rabbit IgG, AP132F, Millipore, Darmstadt, Germany; 1:200). The slices were then washed three times with PBS and mounted with 4’,6-diamidino-2-phenylindole (DAPI)-containing Vectashield (Vector Laboratories ...
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bioRxiv - Molecular Biology 2019Quote: ... and immunoprecipitated overnight at 4 °C with 2 µg affinity-purified rabbit anti-PKM2 antibody raised against a C-terminal peptide (Sigma cat. # SAB4200105, Lot #030M4874) pre-coupled to Dynabeads sheep M-280 anti-rabbit IgG beads (LifeTech) ...
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bioRxiv - Neuroscience 2024Quote: ... Filters were washed three times with TTBS buffer and then incubated for 1 hour with secondary peroxidase-coupled anti-rabbit antibody (1:7000, Millipore, Cat #401393-2 ML). Immunostaining was revealed by enhanced chemiluminescence luminosity (Amersham Pharmacia Biotech ...
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bioRxiv - Neuroscience 2020Quote: Mice were anesthetized using Avertin (2, 2, 2-tribromoethanol; Sigma-Aldrich, 476 mg/kg, i.p) and placed into a stereotactic frame (Kopf) ...
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bioRxiv - Neuroscience 2021Quote: Mice were anaesthetized using Avertin (2, 2, 2-tribromoethanol; Sigma-Aldrich, 476 mg/kg, i.p.) and were surgically implanted with a microdrive (manufactured with the assistance of the Advanced Manufacturing Support Team ...
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bioRxiv - Neuroscience 2020Quote: Mice were anaesthetized using Avertin (2, 2, 2-tribromoethanol; Sigma-Aldrich, 476 mg/kg, i.p.) and were surgically implanted with a microdrive (manufactured with the assistance of the Advanced Manufacturing Support Team ...
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bioRxiv - Cell Biology 2022Quote: ... and 2% glucose) or YPL (0.5% yeast extract, 2% peptone and 2% lactate (Sigma L1375)) ...
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bioRxiv - Bioengineering 2023Quote: Photoinitiator 2-hydroxy-1-[4-(2-hydroxyethoxy) phenyl]-2-methyl-1-propanone (HEPK, Sigma Aldrich) was dissolved into deionized water to obtain an approximately 0.077 wt% solution ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were then blocked in 3% BSA in PBS containing 0.2% Tween 20 before staining with rat anti-HA mAb 3F10 (Sigma, diluted 1:1,000) primary antibody in blocking buffer ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... an NPC1 domain C-targeting mAb were directly labeled with Duolink® In Situ Probemaker PLUS and Duolink® In Situ Probemaker MINUS (Sigma) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Immunology 2021Quote: ... Total cell extracts were prepared and bound to Nunc MaxiSorp plates (eBioscience, Frankfurt, Germany) precoated with murine anti-FLAG mAb at a dilution of 1:2,500 (v/v; Sigma-Aldrich, Munich, Germany) at 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked with 5% bovine serum albumin (BSA) in PBS-T (0.05% Tween 20) and subsequently probed with the rat anti-HA mAb 3F10 (Sigma, 1:1,000 dilution), followed by biotin-conjugated anti-rat antibody (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were incubated for 48 h at 4°C with primary mouse anti-TH antibody (anti-mouse-TH; MAB-318, 1:1000; Millipore, Temecula, CA). The sections were then incubated for 1 h with the appropriate secondary antibody after which sections were washed with PBS and incubated with the required detection kits for development ...
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Proteasome granular localization is regulated through mitochondrial respiration and kinase signalingbioRxiv - Cell Biology 2022Quote: ... and 2-[2-(3-chlorophenyl)hydrazinylidene]-propanedinitrile (CCCP) (Sigma), were used at final concentrations of 0.5 μM ...
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bioRxiv - Microbiology 2019Quote: ... 2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris; Sigma Aldrich), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES ...
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bioRxiv - Immunology 2019Quote: ... 2 mM Glutamax and 50 μM 2-mercaptoethanol (Sigma) (all from Thermo Fisher unless stated otherwise).
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bioRxiv - Cell Biology 2019Quote: ... Around 2–5 μl of 2 mM levamisole (Sigma) was pipetted out on the center of the agarose pads ...
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bioRxiv - Developmental Biology 2020Quote: ... 616452 (2 μM, Sigma-Aldrich, no. 446859-33-2) and penicillin (100 U/mL ...
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bioRxiv - Cell Biology 2020Quote: ... and 1% of photoinitiator (2-hydroxy-2-methylpropiophenone - Sigma) was immediately introduced by capillary action between the PDMS stamp and the glass coverslip ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1 mM 2-mercaptoethanol (2-ME; Sigma-Aldrich, USA), and 4 ng/ml FGF-2 (PeproTech ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... 2-aminoethoxydiphenyl borate (2-APB; Sigma Aldrich, Oakville, ON) experiments ...
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bioRxiv - Biophysics 2021Quote: ... precut 2×2-well culturewell gasket (GBL103250, Sigma, MO) was attached to the coverslip ...
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bioRxiv - Cell Biology 2020Quote: ... 2% (v/v) phosphatase inhibitor cocktail 2 (Sigma-Aldrich) and 500 U/ml universal nuclease ...