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Citations for Millipore Sigma :
1201 - 1250 of 4804 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... MES (CAS No. 145224-94-8) and EDTA (CAS No. 60-00-4) were all purchased from Sigma. PEG-8000 (CAS No ...
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bioRxiv - Cell Biology 2021Quote: ... cells expressing D-amino acid oxidase (DAO) were treated with 10 mM D-alanine (Sigma-Aldrich, #A7377) before the internalization or secretion assays were performed ...
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bioRxiv - Neuroscience 2022Quote: ... 3 washes with D-PBSX (1% vol/vol TritonX-100 - Sigma #T9284-500ml - in D-PBS 1x) were performed ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5 μl of 30 mM D-(-)-2-Amino-5-Phosphonopentanoic acid (D-APV, Sigma Aldrich, Cat #: A8054) dissolved in ACSF (Wang ...
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bioRxiv - Microbiology 2023Quote: ... in 10 mL TSB-D supplemented with 25 µg/ml chlorophenol red-β-d-galactopyranoside (CPRG, Sigma). An OG1RF pCJK205 monoculture (inoculated at 108 CFU/mL ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: Actinomycin D treatment was carried out with 5 μg/mL Actinomycin D (Sigma-Aldrich catalogue number A1410) for the indicated times prior to RNA extraction ...
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bioRxiv - Microbiology 2024Quote: ... D- (+)-3-Phenyllactic acid (D-PLA; 376906-5G) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Lactate and D-PLA were resuspended in sterile water and ILA was mixed with 0.052% v/v DMSO ...
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bioRxiv - Cancer Biology 2024Quote: ... Sigma) or 10µl of 20 µM D-2HG (D-α-Hydroxyglutaric acid disodium salt, catalog # 16859, Sigma) separately ...
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bioRxiv - Microbiology 2023Quote: The effect of cyclo(Pro-Tyr) on ergosterol content was assessed using filipin III staining (Sigma‒Aldrich). An overnight culture of B ...
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bioRxiv - Cell Biology 2024Quote: ... 50 μl of 3 μM TO-PRO-3 or 50 μl 10 μg/mL DAPI (Sigma D9542) in PBS was added ...
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bioRxiv - Neuroscience 2023Quote: ... a 10x pro-inflammatory cocktail 13 was made by combining 5mg/mL IL-1a stock (Sigma #I3901), 10mg/mL TNF stock (Cell Signaling #8902SF) ...
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bioRxiv - Biophysics 2023Quote: ... The pro-tein was then concentrated via Amicon Ultra-15 centrifugal filters (MWCO 10 kDa; Millipore Sigma) and the buffer was exchanged into final buffer (20 mM ammonium acetate buffer at pH 7.0 ...
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bioRxiv - Bioengineering 2023Quote: ... 2) pro-inflammatory M(LPS/IFNγ) (differentiation media supplemented with 100 ng/ml lipopolysaccharide (LPS, Sigma, L4391) and 50 ng/ml interferon gamma (IFNγ ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... the blot was incubated with a mouse anti-His-tag primary antibody (Sigma-Aldrich, Steinheim, Germany), diluted 1:1 000 in TBS (10 mM TRIS/HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... The Trx-His(6) tag was cleaved by addition of 300 units of thrombin (Sigma T4648) directly to the eluate and the solution dialyzed (Slide-A-Lyzer dialysis cassettes ...
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bioRxiv - Plant Biology 2020Quote: ... The His6-SEC5B fusion protein was extracted and purified with Ni-NTA His bind resin (Novagen). The freshly extracted resin-bound GST or GST-XIK proteins were mixed with purified His6-SEC5B protein in protein interaction buffer and incubated at 4°C for 2 h with shaking ...
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bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
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bioRxiv - Plant Biology 2021Quote: ... and GST were fused with a C-terminal octa-His tag in the pET42 vector (Novagen).
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bioRxiv - Biochemistry 2021Quote: ... The latter were then probed with mouse anti-His HRP conjugated antibody (1:400, Sigma-Aldrich) and visualized by chromogenic DAB reagent (Sigma).
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bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with 20 μl His Mag Sepharose Ni beads (Sigma, GE28-9673-88) overnight ...
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bioRxiv - Physiology 2022Quote: ... The F4-2_Fab protein was first purified using cOmplete® His-tag purification resin (Sigma-Aldrich), and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen) ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Biochemistry 2024Quote: ... The UbVs and Ube2d2S22R-Avi were purified by nickel-affinity chromatography with HIS-select resin (Sigma) followed by an overnight 3C digestion using in-house produced 3C protease ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein solution was mixed with Co resin (His-select Cobalt Affinity Gel, Sigma Aldrich) for 1h at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and applied to cOmplete His-tag purification resin (Sigma Aldrich) for 1 hour at 4 °C with rotation ...
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bioRxiv - Microbiology 2024Quote: ... The supernatants were loaded into a Ni-NTA His•Bind® Resin column (Millipore Corp, USA), which had been equilibrated with PBS before use ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was poured over a column with cobalt His-affinity beads (Sigma, St. Louis, MO). The column was washed with the suspension buffer and protein was eluted in a urea and sodium phosphate buffer pH8 with 500mM imidazole ...
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bioRxiv - Molecular Biology 2024Quote: ... Clarified lysate was applied to 2.5 ml bed volume His-select Cobalt Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for an hour prior to application to a gravity flow column ...
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bioRxiv - Plant Biology 2024Quote: ... His6-fusions were affinity purified in batch mode with HIS-select Nickel Affinity beads (Millipore P6611) according to the manufacturer’s protocol for denaturing conditions ...
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bioRxiv - Genetics 2024Quote: ... while the upper section was analyzed by Western blot using an anti-His antibody (Sigma; H1029). The TONSLTPR +ARD and TONSLTPR binding assays were performed as described above with the following modifications ...
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bioRxiv - Molecular Biology 2020Quote: ... in either 4.5 g/L D-glucose or 1 g/L D-glucose + 3.5 g/L L-glucose (Sigma) to serve as an osmotic control ...
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bioRxiv - Bioengineering 2022Quote: Measurements of L- and D-lactic acid were using standard D- and L-lactate assay kits (Sigma-Aldrich) according to manufacturer’s instruction after optimization (Supplementary Fig ...
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bioRxiv - Plant Biology 2020Quote: ... The cultures were then pelleted and resuspended in equal volumes of induction medium (10 mM MgCl2, 10 mM MES, pH 5.6) also containing 100 μM acetosyringone (Sigma) and maintained at room temperature for 4 h ...
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bioRxiv - Biophysics 2021Quote: ... an enzyme mixture was made with 0.1 µM GOx-488 and 0.1 µM GOx-532 in a 50 mM MES (pH 6; Sigma-Aldrich) buffer solution ...
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bioRxiv - Plant Biology 2020Quote: Arabidopsis thaliana seeds were surface sterilized and sown on ½ MS media (Duchefa) with 2.5 mM MES (Sigma; unless otherwise indicated, all chemicals were from Sigma) at pH 5.8 and 1% sucrose ...
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bioRxiv - Plant Biology 2021Quote: ... 0.5% sucrose and 0.8% (w/v) plant agar (Duchefa) which was buffered to pH 5.8 by using 2.5mM 2-Morpholinoethanesulfonic acid (MES) (Sigma-Aldrich). The seeds were stratified at 4°C for 48 hours and grown in in-vitro growth chamber at 21°C under 16 hours of light and 8 hours of darkness (light grown seedlings).
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bioRxiv - Plant Biology 2021Quote: ... bicolor mycelium and poplar seedlings with one or two main roots were transferred onto the surface of low-glucose Pachlewski agar medium (0.1% glucose) containing 0.1% MES (M8250, Sigma, France), and covered by another cellophane membrane (i.e. ...
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bioRxiv - Biochemistry 2021Quote: ... were coated with 10 mM DOPA-Tet and incubated for 5 days at 37 °C in 1 mL of either 50 mM MES pH 4.5 (Millipore-Sigma), 100 mM MES pH 6.0 ...
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bioRxiv - Immunology 2021Quote: PBMCs from donors previously screened for ex vivo CD4+ T cell reactivities against MHC-class II peptides were thawed and rested in T cell medium (TCM; IMDM + 1x P/S + 50μM β-ME + 10% h.i. HS) containing 1μg/mL DNase I (Sigma-Aldrich) at a concentration of 2-3×106 cells/mL for 3h at 37°C and 7.5% CO2 ...
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bioRxiv - Plant Biology 2024Quote: ... They were then resuspended in 10 mM MES-KOH buffer (pH 5.7) with 10 mM MgCl2 and 100 mM acetosyringone (49-hydroxy-3′,5′-dimethoxyacetophenone; Sigma) to achieve a final OD600 of 0.5 ...
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bioRxiv - Plant Biology 2023Quote: ... individual 7-day-old Arabidopsis seedlings were placed in white 96-well plates filled with 200 µl reconstitution buffer (2.5 mM MES pH 5.7 [Sigma-Aldrich, Taufkirchen ...
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bioRxiv - Cell Biology 2023Quote: ... iPSCs and ESC were cultured in ‘Enhanced medium’ consisting of MES medium supplemented with 0.5µmol/l VPA (P4543-10G, Sigma-Aldrich), 1.5µmol/l CGP77675 (SML0314 ...
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bioRxiv - Immunology 2023Quote: ... in culture medium (RPMI 1640 + 10% FBS, 10% penicillin/streptomycin, 50mM 2-ME (Sigma-Aldrich, Cat# 60-24-2), and 1000x BD Golgi Plug containing Brefeldin A (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... Seeds were chlorine gas-sterilised and plated on ½ Murashige and Skoog (MS) basal medium (Duchefa Biochemie) with 0.025% MES (Sigma, pH5.7 and 1.2% agar (Duchefa Biochemie) ...
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bioRxiv - Genomics 2023Quote: pSM44 mES cells were grown at 37C under 7% CO2 on plates coated with 0.2% gelatin (Sigma, G1393-100ML) and 1.75 mg/mL laminin (Life Technologies Corporation ...
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bioRxiv - Plant Biology 2024Quote: ... The pH of the media was set to 5.7 by NaOH which was buffered by using 0.05% (w/v) 2-(N-morpholino)ethanesulfonic acid (MES) (Sigma-Aldrich). Sterilized seeds were then put on media and stratified at 4°C for 2 days in darkness and then light induced for 6 hours in white light at 21°C ...
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bioRxiv - Microbiology 2020Quote: ... and 1 mM D-lactoylglutathione (Sigma L7140) were monitored in a 96-well plate for an increase in absorbance at 412 nm ...