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Citations for Millipore Sigma :
1451 - 1500 of 4804 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... + 100 µg/ ml cytochalasin D (Sigma C8273). For packing ...
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bioRxiv - Physiology 2024Quote: ... and 0.48g component D (Sigma-Aldrich, USA). For the minimal resin embedding of cell monolayers on sapphire discs we used a modified approach48 ...
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bioRxiv - Neuroscience 2024Quote: ... and poly-D-lysine (Sigma Aldrich, P7886) coated 25 mm glass coverslips (VWR International Ltd ...
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bioRxiv - Neuroscience 2024Quote: ... 10 D-glucose (Sigma-Aldrich, Cat # G7021), and 1MgCl2 (Merck ...
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bioRxiv - Plant Biology 2024Quote: ... 50 μM of actinomycin D (Sigma A9415) was added to the cell wall digestion solution ...
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bioRxiv - Molecular Biology 2024Quote: ... IPTG (isopropyl-β-D-thiogalactopyranoside) (Sigma-Aldrich) was added to the bacteria culture to a final concentration of 0.5 mM to induce protein expression at 16°C with shaking at 220 RPM for 20 h ...
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bioRxiv - Neuroscience 2024Quote: ... and D-tubocurarine chloride (30 μM; Sigma), similar to a previous report (González-Forero and Alvarez ...
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bioRxiv - Immunology 2024Quote: ... isopropyl β-D-1-thiogalactopyranoside (IPTG, Sigma) was added to a final concentration of 0.7 mM for overnight induction at 25°C ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mg/ml collagenase-D (Sigma-Aldrich), 0.5 mg/ml DNase-I (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2024Quote: ... 20g/L alpha-D-glucose (Sigma Aldrich). YPD media was used when selection was not required (20g/L bacto peptone ...
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bioRxiv - Immunology 2024Quote: ... 7- aminoactinomycin D (7-AAD) (Sigma-Aldrich) was used ...
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bioRxiv - Neuroscience 2024Quote: ... 30% D (+)-Glucose (Sigma-Aldrich, cat. #G7021), 1 M NaHCO3 (Sigma-Aldrich ...
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bioRxiv - Biophysics 2024Quote: ... 0.4 mM isopropyl-β-D-thiogalactopyranoside (Sigma) and 0.1 mM Zn(CH3CO2)2 were added ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.6% w/v D-glucose (G8769, Sigma), 5mM HEPES (15630056 ...
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bioRxiv - Bioengineering 2024Quote: ... were coated with poly-D-lysine (Sigma), human monoclonal anti-CD3 antibody (5µg/mL or 10µg/mL ...
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bioRxiv - Biophysics 2024Quote: ... and D-agonist SNC-80 (Sigma-Aldrich) were applied to the cells as described in the companion paper ...
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bioRxiv - Cell Biology 2024Quote: ... coated by poly-D-lysine (Sigma-Aldrich) for at least 16 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Actinomycin D (Sigma-Aldrich, St Louis, MO), drug vehicle (dimethyl sulfoxide [DMSO]) ...
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bioRxiv - Cell Biology 2024Quote: ... Actinomycin D (ActD; 2 μg/ml, Sigma), MLN-4924 (NEDDi ...
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bioRxiv - Developmental Biology 2024Quote: ... in KSOM medium (MR-101-D, Sigma). Embryos were scored at blastocyst stage immediately before moment of hatching for contribution of TdTomato+ ESCs to either ICM and/or TE ...
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bioRxiv - Biochemistry 2024Quote: ... naturally-occurring sodium D-βOHB (Sigma, 298360), naturally-occurring ethylacetoacetate (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... The signal was detected by the Pro e-BLOT Touch Imager using chemiluminescent substrate (WBKLS0500; Merck Millipore, Boston), and the band intensity was measured using ImageJ software (National Institutes of Health).
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bioRxiv - Biochemistry 2024Quote: ... pH 5.6) containing 600 mg Vinotaste Pro (Novozymes) and 0.5 mg chitinase from Streptomyces griseus (Sigma-Aldrich C6137). This mixture was incubated in a sterile petri dish in an orbital shaker at 60 rpm and 30 °C for approx ...
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bioRxiv - Neuroscience 2021Quote: Rats were subcutaneously injected with NMDA receptor co-agonist D-SER (s.c. D-SER 10mg/Kg, Sigma-Aldrich, Ireland) in vehicle ...
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bioRxiv - Microbiology 2021Quote: ... D-glucose (5 mg.ml−1) or nothing (no-amendment control) (all amendment chemicals were from Sigma–Aldrich, except D(+)-galactose which was purchased from Carl Roth GmbH) ...
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bioRxiv - Cell Biology 2022Quote: ... D-PBS was replaced with ice-cold blocking solution (3% BSA [Sigma, A3803] in D-PBS, without Ca2+/Mg2+) using a Dragonfly Discovery liquid dispenser (SPT Labtech ...
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bioRxiv - Neuroscience 2024Quote: ... 5 μM Emricasan (Cat.# 7310, R&D), 1× penicillin-streptomycin, 0.7 μM ISRIB (Cat.# 5284, R&D) and 1× polyamines (Cat.# P8483, Sigma) and incubated for 3 days ...
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bioRxiv - Cancer Biology 2024Quote: ... with drinking water also supplemented with 23.1 g/L of D-fructose and 18.9g/L D-glucose (Sigma-Aldrich) for 7 ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: Recombinant human MMP-2 (R&D) and MMP-9 (R&D) were activated using p-aminophenylmercuric acetate (APMA, Sigma) in MMP assay buffer (50 mM Tris (Fisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then washed with D-PBS with calcium and magnesium (named D-PBS from here on in, Sigma) and cells then removed by incubation with pre-warmed extraction buffer (20mM NH4OH ...
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bioRxiv - Biochemistry 2020Quote: ... Clarified lysate was loaded onto a gravity column containing 1 mL HIS-Select Nickel Affinity Gel (Sigma) pre-equilibrated with Resuspension Buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM tris (2-carboxyethyl) phosphine (TCEP) and P8849 Protease Inhibitor Cocktail for His-tagged proteins (Sigma). The lysate was cleared at 25.000 × g for 30 min at 4°C and the supernatant was loaded on Protino®Ni-TED 2000 packed columns and purified as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Cancer Biology 2021Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2×YT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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bioRxiv - Biochemistry 2021Quote: Genes for His-tagged or Strep II-tagged nanobodies were cloned into the pET 26b vector (Novagen). The expression and purification of all His-tagged nanobodies have been described previously 12 ...
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bioRxiv - Microbiology 2020Quote: ... and the supernatants were independently applied to a column of HIS-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with 50 mM sodium phosphate buffer ...
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bioRxiv - Biophysics 2021Quote: ... followed by cleavage of the His-tag with 3C protease and dephosphorylation with bovine alkaline phosphatase (Sigma). Further purification was performed by ion exchange chromatography (Source Q for WT and the S897E ...
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bioRxiv - Biochemistry 2022Quote: ... and another was loaded with 10 ng of human HSP70 (His tagged human HSP70, SRP5190, Millipore Sigma), which allowed comparisons to be made among separate gels ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were detected using the primary antibody (Mouse α-His at 1:2500 dilution, Sigma Aldrich) followed by the HRP conjugated secondary antibody (Sheep α-Mouse at 1:7000 dilution ...
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bioRxiv - Biophysics 2020Quote: ... and passed through pre-equilibrated HIS-Select Ni-nitrilotriacetic acid resin (Ni-NTA) (Sigma-Aldrich Co., USA) at 4 °C for binding of 6x-His tagged protein ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used: anti-His (1: 2000, Cat No: 70796-4, Novagen, Madison, WI); anti-DNALI1 (1 ...
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bioRxiv - Biochemistry 2021Quote: ... pneumoniae LicB with an N-terminal 10×His affinity tag in a modified pET-19b vector (Novagen) was overexpressed in E ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
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bioRxiv - Cell Biology 2020Quote: ... in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich, #U5507), and ≈ 2 μg of purified recombinant mouse GST-Trim39 (WT ...
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bioRxiv - Biophysics 2021Quote: ... The His-eluent was concentrated using a 30 kDa cutoff Amicon Ultra-4 centrifugal filter (Millipore UFC803008), filtered using a 0.22 μm Millex-GP PES membrane (Millipore SLGP033RS) ...
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bioRxiv - Immunology 2022Quote: ... the 6x HIS tag region of the recombinant protein was removed following the vector manufacturers specifications (Novagen). This recombinant SmCI-1 was used to generate a rabbit anti-Sm-CI-1 polyclonal antibody using the Custom pAb service offered by Genscript.
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bioRxiv - Microbiology 2022Quote: ... with the following modifications: Mouse anti-GST tag or mouse anti-His tag 1:200× (Sigma-Aldrich) and HRP-conjugated goat anti-mouse IgG 1:2,000× (Seracare Life Sciences ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Cancer Biology 2023Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2xYT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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bioRxiv - Genetics 2023Quote: ... imidazole was removed from the eluted His-SIRT5 using ultra-0.5 centrifugal filter units (UFC501096, Merck Millipore). 500 µl of the recombinant SIRT5 elution fractionation was added to the ultra-0.5 centrifugal filter units and centrifuged at 14,000 x g for 15 min at room temperature ...