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Citations for Millipore Sigma :
1201 - 1250 of 10000+ citations for 6 Chloro N methyl pyrimidine 4 5 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... followed by fixation in 4% formaldehyde (EMD Millipore FX0410-5) in 1X PBS with Triton X-100 (0.3% ...
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bioRxiv - Biophysics 2021Quote: ... 500 µl of the gel solution was mixed rapidly with 2 µl of N,N,N’,N’-Tetramethylethylenediamine (Sigma-Aldrich) and quickly added to the sample coverslip ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated in ACSF containing membrane-permeant AM variant of the fast exogenous Ca2+ chelator 1,2-Bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM, 10 µM, Sigma-Aldrich) for 30 min and then returned to normal ACSF for another 30 min before recording ...
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bioRxiv - Biochemistry 2020Quote: Full length human KBP residues 1-621 in a PSTCm1 expression vector (with kanamycin resistance and a N-terminal thrombin cleavable 6 x His-tag) was expressed in Rosetta2 cells (Novagen). Following immobilised metal-affinity chromatography with Ni-NTA resin (Qiagen) ...
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bioRxiv - Neuroscience 2020Quote: ... two brains were pooled (n=6) and homogenized in 300 µL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich). Tissue preparation and biochemical analysis followed protocols described in (Sachett et al. ...
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bioRxiv - Neuroscience 2021Quote: ... three brains were pooled (n=6) and homogenized in 450 μL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich) and centrifuged at 10,000 g at 4 °C in a cooling centrifuge ...
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bioRxiv - Neuroscience 2021Quote: ... four brains were pooled (n=6) and homogenized in 600 μL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich) and centrifuged at 10,000 g at 4 °C in a cooling centrifuge ...
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bioRxiv - Biophysics 2022Quote: ... in this work referred to as Pif1) fused at its N-terminus to a 6-histidine tag was cloned into vector pET28 (Novagen) and transformed into E ...
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bioRxiv - Pathology 2021Quote: ... rats in the high-dose 7-d L-DOPA priming group (n=6) were treated daily with L-DOPA (12 mg/kg) + benserazide (15 mg/kg, s.c., Sigma-Aldrich) for 7 d (i.e. ...
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bioRxiv - Cell Biology 2019Quote: ... and plasma (n=6) of the experimental groups were filtered with a 30 kDa cut-off Microcon filter (Merck Millipore, Tullagreen Carrigtwohill Co.Cork ...
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Molecular Biology 2019Quote: ... was fused with an N-terminal His(6)-tag followed by maltose binding protein (MBP) tag and TEV cleavage site in pET21 vector (Novagen).
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bioRxiv - Neuroscience 2023Quote: ... HEK cells were washed three times with ice-cold PBSx1 and lysed using Maltoside lysis buffer [1% N-dodecyl maltoside (cat no. 69227-93-6 – Sigma) dissolved in PBSx1 with freshly added 0.1 mg/ml PMSF and protease inhibitors] ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Genomics 2019Quote: ... 1.5 g/L (days 0-5 and days 11-28) or 2.5 g/L (days 6-10) NaHCO3 (Sigma-Aldrich), and 0.25 mM (days 3-10 ...
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bioRxiv - Developmental Biology 2020Quote: ... 1.5 g/L (days 0-5 and days 11-14) or 2.5 g/L (days 6-10) NaHCO3 (Sigma-Aldrich), and 0.25 mM ascorbic acid (days 3-10) ...
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bioRxiv - Immunology 2021Quote: ... or 0.5 μM/ml 5-amino-6-D-ribitylaminouracil (5-A-RU) (courtesy of Jeffrey Aubé, UNC) and 50 μM/ml Methylglyoxal (Sigma). Brefeldin A Solution (Biolegend ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.5% 3-(N,N-Dimethylmyristylammonio)propanesulfonate (Sigma T7763), 200 μg/mL RNase A ...
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Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... 0.5% 3-(N,N-Dimethylmyristylammonio)-propanesulfonate (Sigma, T7763), 200µg/mL RNAse A ...
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bioRxiv - Genomics 2020Quote: ... 10% N,N-dimethylformamide (Sigma, Cat. No. D4551), 4 × Protease Inhibitor (Promega ...
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bioRxiv - Bioengineering 2020Quote: ... resuspended in N,N-dimethylformamide (Sigma-Aldrich D4551) with a 30-fold molar excess of the ligand ...
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bioRxiv - Microbiology 2020Quote: ... was dissolved in N,N-dimethylformamide (Merck Millipore) and used for peroxidase-dependent staining ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1% (wt/wt) N,N’-methylenebisacrylamide (BIS, Sigma) in PBS supplemented with 0.5% ammoniumpersulfate and 0.5% tetramethylethylendiamine (TEMED ...
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bioRxiv - Biophysics 2020Quote: ... 10 mL N,N-Dimethylformamide (DMF, Sigma-Aldrich) and 10 mL ethanol were added to this liquid resulting in the formation of a clear ...
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bioRxiv - Biophysics 2020Quote: ... N,N’-methylen-bisacrylamide (BIS, 2%, M1533, Sigma), N,N,N′,N′-Tetramethylethylenediamine (TEMED ...
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bioRxiv - Cell Biology 2022Quote: ... 200 μM N,N-dicyclohexylcarbodiimide (DCCD) (Sigma, D80002) and 30 μM carbonyl cyanide m-chlorophenyl hydrazone (CCCP ...
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bioRxiv - Biophysics 2021Quote: ... 0.15% (wt/wt) N,N′-methylenebisacrylamide (M7279, Sigma), 8.625% (wt/wt ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1% (wt/wt) N,N’-Methylenebisacrylamide (BIS, SIGMA) in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.1% (w/v) N,N′-methylenebisacrylamid (Sigma-Aldrich) in 1× PBS supplemented with 0.5% APS (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... N,N′-Methylenebisacrylamide (bisacrylamide, Sigma, Cat#M1533-25ML), Sodium acrylate (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... 0.15% (w/v) N,N’-Methylenebisacrylamide (Sigma-Aldrich), 11.7% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... N,N-Dimethylromaid and Triton X (Sigma Aldrich). A counterstain was carried out with Mayer’s Hemalaun solution (Merck) ...
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bioRxiv - Cell Biology 2023Quote: ... N-N’-methylenebisacrylamide 1.5 % (w/v) (M7279, Sigma) and NaCl 11.7 % (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... N-N’-methylenebisacrylamide 1.5% (w/v) (M7279, Sigma) and NaCl 11.7% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... N-N’-methylenebisacrylamide 0.1% (w/v) (M7279, Sigma) in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... 0.15% (w/v) N,N′-methylenebisacrylamide (Sigma, M7279) and 8.625% (w/v ...
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bioRxiv - Neuroscience 2020Quote: ... were performed with MTT (3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) (Sigma-Aldrich) as described in Sanz et al ...
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bioRxiv - Biochemistry 2019Quote: ... 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranosyl 5’-monophosphate (ZMP; Sigma Aldrich, St. Louis, MO) at a stock concentration of 50 mM in dimethyl sulfoxide (DMSO ...
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bioRxiv - Immunology 2023Quote: ... 4 weeks after BM transplantation animals were injected with 5-FU (5-fluorouracil F6627, Sigma-Aldrich) at a dose of 150 mg/kg in 100ul of PBS ...
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bioRxiv - Immunology 2024Quote: ... followed by 4 - 5 hours of stimulation by phorbol myristate acetate (PMA, 5 ng/ml, Sigma) and ionomycin (500 ng/ml ...
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bioRxiv - Genomics 2020Quote: ... followed by 5 washes with washing buffer containing 0.05% Tween-20 (Sigma-Aldrich, Cat. N: P9416) and two additional washes with low-TE water ...
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bioRxiv - Developmental Biology 2022Quote: ... randomly selected animals (n=18) were treated with T3 (3,3’,5-Triiodo-L-thyronine – Sigma T2877) twice a week in Holtfreter’s solution with a 25 nM final concentration ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 5 mM of N-hydroxysulfosuccinimide sodium salt (NHS, 24500-Sigma-Aldrich, St. Louis, MO, USA) were added directly to 1 mL of insulin (11061-Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... The filtered sample was loaded at 0.5 mL/min onto an AKTA FPLC system fitted with either a Mono S 5/50 GL column or freshly prepared 5 mL fine mesh cellulose phosphate column (Sigma P/N C2258) equilibrated in binding buffer containing 50 mM HEPES (pH 7.2 ...
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bioRxiv - Microbiology 2020Quote: ... polyoxyethylene(4)lauryl ether (Brij L4®), sodium tetraborate decahydrate (≥99.5%) and N,N,N,N-tetramethyl-ethylenediamine (TEMED, 99%) were purchased from Sigma Aldrich, US ...
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bioRxiv - Neuroscience 2022Quote: ... For zinc chelation N,N,N,N-Tetrakis (2-pyridylmethyl) ethylenediamine (TPEN) (Cat# P4413-50MG, Sigma-Aldrich, St Louis, MO, USA) solution was freshly prepared in 10%-ethanol (Cat#100983 ...
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bioRxiv - Neuroscience 2022Quote: ... ethylene glycol-bis(β-aminoethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA; Cat#E3889, Sigma-Aldrich, St Louis, MO, USA) 1 mM ...
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bioRxiv - Biochemistry 2023Quote: ... fully oxidized and fully reduced samples were generated by the addition of 20 mM N,N,N′,N′-Tetramethylazodicarboxamide (diamide, Sigma Aldrich) or 100 mM Dithiotreitol (DTT ...
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bioRxiv - Neuroscience 2023Quote: ... Embryos were washed 1 hour x6 and then transferred to CUBIC1 solution (25 wt% N,N,N’,N’-tetrakis(2-hydroxypropyl)ethylenediamine (Sigma 122262), 25 wt% urea and 15 wt% Triton X-100 ...
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bioRxiv - Neuroscience 2021Quote: ... ablation efficiency was confirmed using the fluorescent dye 2-4-(dimethylamino)styryl-N-ethylpyridinium-iodide (DASPEI)(Sigma). Larvae were immersed in 0.005% DASPEI in E3 for 15 min ...