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Citations for Millipore Sigma :
1251 - 1300 of 10000+ citations for 6 Chloro N methyl pyrimidine 4 5 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The DNA content was assessed by DAPI (4′,6-diamidino-2-phenylindole; Sigma-Aldrich) staining ...
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bioRxiv - Systems Biology 2023Quote: ... After labeling the nuclei with 4’,6-diamid-ino-2-phenylindole (DAPI; Sigma-Aldrich), the cells were mounted with Mowiol 40-88 (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... After staining nuclei with DAPI (1:1000, 4’, 6-diamidino-2-phenylindole dihydrochloride; Sigma) and several washes with PBST ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were counterstained with 4’,6’-diamidino-2-phenylindole (DAPI) (1:3000; Sigma-Aldrich), and coverslips were mounted with FluoroGel (Electron Microscopy Services ...
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bioRxiv - Microbiology 2023Quote: ... and DAPI (4=,6-diamidino-2-phenylindole) were purchased from Sigma (St. Louis, MO). MG132 was purchased from Calbiochem (San Diego ...
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bioRxiv - Bioengineering 2023Quote: ... and collagen were stained with 4′,6-diamidino-2-phenylindole (DAPI (Sigma-Aldrich, D9542), diluted to 0.1 μg/ml in PBS) ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were stained with either DAPI (4′,6-diamidino-2-phenylindole, Sigma-Aldrich F6057) for glass coverslip or Hoechst 33342 (ThermoFisher 62249 ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were counterstained with 4’,6’-diamidino-2-phenylindole (DAPI) (1:3000; Sigma-Aldrich) and coverslips were mounted with FluoroGel (Electron Microscopy Services ...
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bioRxiv - Developmental Biology 2023Quote: ... counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Sigma, 1:2000 in 1× PBS) for 5’ and mounted with fluorescent mounting medium (Dako) ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were counterstained with a 4’,6-diamidino-2-phenylindole solution (DAPI; Sigma-Aldrich). After four washes with TBST ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; D9542, Sigma-Aldrich) for 5 min at 1:1000.
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bioRxiv - Immunology 2024Quote: ... Cell nuclei were counter-stained with 4’,6-diamino-2-phenylindole (DAPI, Sigma-Aldrich). Another randomly chosen series was used for Nissl staining in order to determine the total volume of the granular cell layer (GCL ...
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bioRxiv - Immunology 2024Quote: ... a 5min incubation at RT with 4’,6-diamino-2-phenylindole (DAPI, Sigma-Aldrich); and three 5min washes with PBS ...
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bioRxiv - Microbiology 2024Quote: ... at 4 °C in a 6–30% OptiPrep (Sigma-Aldrich, MilliporeSigma, Burlington, MA, USA) density gradient ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were stained with 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich, D9542) mounted on the objective slides ...
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bioRxiv - Immunology 2024Quote: ... additional staining with 4’,6-diamidino-2-phenylindole (DAPI, 100 ng/ml; Sigma-Aldrich) was performed ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were treated with cholesterol (3β-hydroxy-5-cholestene, Sigma-Aldrich, C-3045; cholesterol–methyl-β-cyclodextrin, Sigma-Aldrich, C-4951, Missouri, USA), IL1β (GenScript ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe (strain SHY1058) cultures were labeled with 5 mM 4-thiouracil (4-thioU) (Sigma-Aldrich #440736) for 4 min ...
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bioRxiv - Microbiology 2020Quote: ... After 23 h, cells were fixed with 4% paraformaldehyde and stained with DAPI (4’, 6-diamidino-2-phenylindole) (Sigma-Aldrich, USA). Nine fields per well were imaged on an Operetta high-content imaging system (PerkinElmer) ...
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bioRxiv - Bioengineering 2022Quote: ... the tissues were incubated overnight at RT and in dark conditions with a 1/400 dilution of Myosin 4 Monoclonal Antibody (MF20; eBioscience) as primary antibody and 4′,6-diamindino-2-phenylindole (DAPI; Sigma-Aldrich) at 1:5000 ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were fixed with 4% paraformaldehyde for 10 min and treated with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) diluted to a 1/10,000 concentration from the stock solution ...
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bioRxiv - Plant Biology 2020Quote: ... 150 µM 3′,5′-Dimethoxy-4′-hydroxyacetophenone (acetosyringone; SIGMA, USA) at a 23.7×108 cfu/ml (OD600=0.75 ...
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bioRxiv - Molecular Biology 2021Quote: ... to YPD containing 5 mM 4-thiouracil (Sigma, 440736-1G). Cells were incubated with 4tU for 5 minutes before pelleting (one minute ...
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bioRxiv - Synthetic Biology 2020Quote: ... 100 µM acetosyringone (3′,5′-dimethoxy-4′-hydroxyacetophenone, Sigma D134406), and incubated for 6h at 28°C at 150 rpm.
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bioRxiv - Neuroscience 2021Quote: ... 5 dpf larvae were fixed in 4% paraformaldehyde (PFA; Sigma) in phosphate-buffered saline (PBS ...
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bioRxiv - Plant Biology 2020Quote: ... 200 μM 3′,5′-Dimethoxy-4′-hydroxyacetophenone (acetosyringone) (SIGMA, USA) carrying the corresponding binary plasmids (Table 1) ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich), and 0.5 μg/ml anti-CD180 (RP/14 ...
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bioRxiv - Neuroscience 2022Quote: ... 100μm of 4-AP (Sigma-Aldrich, CAS#: 504-24-5) was washed on for 3 minutes (the amount of time it took to regularly induce 4-AP oscillations ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 ng/ml mouse recombinant IL-4 (Sigma-Aldrich) to allow B cell activation and class switch recombination to IgG1 ...
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bioRxiv - Cancer Biology 2023Quote: ... and 4 µM 5-fluoro-2′-deoxyuridine (FldU, Sigma-Aldrich) were added to the cells ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by fixation in 4% formaldehyde (EMD Millipore FX0410-5) in 1X PBS with Triton X-100 (0.3% ...
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bioRxiv - Immunology 2024Quote: ... or 4 mM EGTA (CAS 67-42-5, Sigma-Aldrich) were added to buffers as outlined in the text.
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bioRxiv - Biophysics 2021Quote: ... 500 µl of the gel solution was mixed rapidly with 2 µl of N,N,N’,N’-Tetramethylethylenediamine (Sigma-Aldrich) and quickly added to the sample coverslip ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated in ACSF containing membrane-permeant AM variant of the fast exogenous Ca2+ chelator 1,2-Bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM, 10 µM, Sigma-Aldrich) for 30 min and then returned to normal ACSF for another 30 min before recording ...
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bioRxiv - Microbiology 2020Quote: ... The internal standard was 1 μL of methyl heneicosanoate (10 mg/mL) and Bacterial acid methyl ester (BAME) mix (Merck-Millipore, Burlington, MA, USA) was used to identify the each peak of fatty acids and analytical standards for each fatty acid were used for quantification.
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bioRxiv - Biochemistry 2020Quote: Full length human KBP residues 1-621 in a PSTCm1 expression vector (with kanamycin resistance and a N-terminal thrombin cleavable 6 x His-tag) was expressed in Rosetta2 cells (Novagen). Following immobilised metal-affinity chromatography with Ni-NTA resin (Qiagen) ...
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bioRxiv - Neuroscience 2020Quote: ... two brains were pooled (n=6) and homogenized in 300 µL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich). Tissue preparation and biochemical analysis followed protocols described in (Sachett et al. ...
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bioRxiv - Neuroscience 2021Quote: ... three brains were pooled (n=6) and homogenized in 450 μL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich) and centrifuged at 10,000 g at 4 °C in a cooling centrifuge ...
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bioRxiv - Neuroscience 2021Quote: ... four brains were pooled (n=6) and homogenized in 600 μL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich) and centrifuged at 10,000 g at 4 °C in a cooling centrifuge ...
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bioRxiv - Biophysics 2022Quote: ... in this work referred to as Pif1) fused at its N-terminus to a 6-histidine tag was cloned into vector pET28 (Novagen) and transformed into E ...
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bioRxiv - Pathology 2021Quote: ... rats in the high-dose 7-d L-DOPA priming group (n=6) were treated daily with L-DOPA (12 mg/kg) + benserazide (15 mg/kg, s.c., Sigma-Aldrich) for 7 d (i.e. ...
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bioRxiv - Cell Biology 2019Quote: ... and plasma (n=6) of the experimental groups were filtered with a 30 kDa cut-off Microcon filter (Merck Millipore, Tullagreen Carrigtwohill Co.Cork ...
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Molecular Biology 2019Quote: ... was fused with an N-terminal His(6)-tag followed by maltose binding protein (MBP) tag and TEV cleavage site in pET21 vector (Novagen).
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bioRxiv - Neuroscience 2023Quote: ... HEK cells were washed three times with ice-cold PBSx1 and lysed using Maltoside lysis buffer [1% N-dodecyl maltoside (cat no. 69227-93-6 – Sigma) dissolved in PBSx1 with freshly added 0.1 mg/ml PMSF and protease inhibitors] ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.5% 3-(N,N-Dimethylmyristylammonio)propanesulfonate (Sigma T7763), 200 μg/mL RNase A ...
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Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... 0.5% 3-(N,N-Dimethylmyristylammonio)-propanesulfonate (Sigma, T7763), 200µg/mL RNAse A ...
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bioRxiv - Genomics 2020Quote: ... 10% N,N-dimethylformamide (Sigma, Cat. No. D4551), 4 × Protease Inhibitor (Promega ...
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bioRxiv - Bioengineering 2020Quote: ... resuspended in N,N-dimethylformamide (Sigma-Aldrich D4551) with a 30-fold molar excess of the ligand ...