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Citations for Millipore Sigma :
1151 - 1200 of 10000+ citations for Synaptic Ras GTPase Activating Protein 1 Syngap1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... anti-Ubiquitinylated proteins,clone FK2 (cat# 04-263, Merck Millipore; 1:1000). Ponceau solution was prepared with Ponceau BS (cat# B6008 ...
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bioRxiv - Genetics 2021Quote: ... clarification and benzonase treatment (250 u/1 mg protein, SIGMA E1014-5KU) extracts were incubated 1h at 4°C with 50 µL magnetic beads (Dynabeads M-280 sheep anti-mouse IgG ,invitrogen 11202D ...
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bioRxiv - Biochemistry 2021Quote: ... Bound protein was detected using anti-His-HRP conjugate (Sigma; 1:10,000). All proteins and antibodies were in 0.1 M phosphate buffer ...
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bioRxiv - Biophysics 2020Quote: Proteins were buffer exchanged into 1 M aqueous ammonium acetate (Sigma-Aldrich) using P6 Biospin columns (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of total protein was added to 7.8 nmol nitrocefin (Sigma) and absorbance at 495 nm measured kinetically for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... protein expression was induced using 1 μg/mL of doxycycline (Sigma, D9891) for 16 h.
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bioRxiv - Evolutionary Biology 2022Quote: ... Proteins were probed with rabbit anti-HA (1:1,000 Sigma-Aldrich, H3663) or anti-β-actin as loading control (1:10,000 Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Proteins were probed with mouse anti-FLAG (1:5,000 Sigma-Aldrich, F3165) or anti-β-actin (1:5,000 Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Immunohistochemistry was performed using glial fibrillary acidic protein (GFAP) (1:1000; Millipore) and ionized Ca2+-binding adapter protein (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-MBP (Myelin Basic Protein, rat polyclonal, 1:1000 Millipore MAB 386) or antimouse IgG (Biotin-SP AffiniPure ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... anti-microtubule-associated protein 2 (MAP2) Alexa 555 (1:500, MAB3418A5; Millipore), antimicrotubule-associated protein 2 (MAP2 ...
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bioRxiv - Cell Biology 2022Quote: ... DNA-protein complexes were crosslinked using 1% final concentration of formaldehyde (Sigma) for 10min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-microtubule-associated protein 2a+b (MAP2, Sigma #M1406; 1:2000), rabbit anti-tyrosine hydroxylase (TH ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse Anti-Glial Fibrillary Acidic Protein (1:100, Millipore, Cat# MAB360, RRID:AB_11212597), Mouse Anti-NeuN (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-amyloid precursor protein (APP, C-terminal, 1:1000, Sigma, #A8717), rat anti-green fluorescent protein (GFP ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested overnight at 37C with 1:50 trypsin (Sigma-Aldrich) and 1:75 and lysyl endopeptidase (Wako) ...
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bioRxiv - Biochemistry 2022Quote: ... protein expression was induced with 1 μg/mL doxycycline hyclate (Sigma Aldrich), and the culture was placed in a shaking incubator maintaining 37°C and a 5% CO2 atmosphere ...
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bioRxiv - Bioengineering 2023Quote: ... mouse monoclonal anti-glial fibrillary acidic protein (1:1000, Millipore Sigma, #MAB3402), rat monoclonal anti-CD45 (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were precipitated with 1:4 trichloroacetic acid solution (TCA, Sigma Aldrich) and washed with acetone ...
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bioRxiv - Cell Biology 2023Quote: ... and 4 g digitonin:1 g mitochondrial protein (Sigma-Aldrich, #D141-100MG). Mitochondria were incubated on ice for 20 minutes and then centrifuged at 20,000 x g for 10 minutes at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... protease incubation (Pepsin Porcine, 1:50, enzyme to protein ratio, Sigma-Aldrich) was performed for 4 h at 37 °C in 40 mM HCl in a total volume of 2 ml (pH 2) ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-glial fibrillary acidic protein (GFAP, 1:1000, Millipore Sigma, USA), anti-NeuN (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... in the presence of protein A-sepharose beads (1/20 µl, Sigma). Immune complexes eluted were separated on 4-20% SDS PAGE gels (mini protean TGX ...
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bioRxiv - Biochemistry 2024Quote: ... mouse monoclonal anti-mitochondrial surface protein p60 (Millipore, MAB1273, IB 1:1,000), rabbit polyclonal anti-HA (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... antibodies were added at 1:100 dilution in 1% saponin (Sigma-Aldrich, USA) supplemented with 5% FBS (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The primary antibodies (PV; 1:1000, Swant; Biotin-WFA; 1:100, Sigma-Aldrich) were incubated over night at 4 °C following rinsing tissues for 5 min three times in PBS-T (0.2 % Triton-X in PBS) ...
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bioRxiv - Microbiology 2021Quote: ... p62 antibody (#P0067; WB 1:5000; IF 1:1000) was purchased from Sigma. Hoechst Trihydrochloride Trihydrate 33342 (Invitrogen #H3570 ...
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bioRxiv - Molecular Biology 2020Quote: ... non-fixed cells and the TRA-1-60 antibody (MAB4360, Millipore, 1:100) were used ...
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bioRxiv - Bioengineering 2020Quote: ... Primary antibodies were Tuj-1/beta-III tubulin (T8578, 1:500, Sigma-Aldrich) to label axons and synapsin-1 (A6442 ...
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bioRxiv - Cell Biology 2021Quote: ... The following antibodies were used: mouse anti-Tau-1 (1:400, Millipore Sigma), rabbit anti-ubiquitin (1:500 ...
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bioRxiv - Genomics 2020Quote: ... Primary antibodies (APP-Y188, Abcam ab32136, 1:4,000; Tubulin, Sigma T5168, 1:4000) were diluted in blocking solution and incubated with the membrane overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... and mouse monoclonal anti-NUCB2/nesfatin-1 antibody (1:50; Merck Millipore, MABS1164), and b ...
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bioRxiv - Developmental Biology 2022Quote: ... Mouse anti-myc antibody (1 μg) or mouse IgG (1 μg) (EMD Millipore) were incubated with protein A/G Magna beads (20 μl ...
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bioRxiv - Physiology 2024Quote: ... Secondary antibodies were peroxidase-conjugated anti-mouse (1:20,000, Dianova; 1:10,000, Sigma), anti-rat (1:10,000 Dianova ...
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bioRxiv - Cancer Biology 2023Quote: ... and primary vinculin antibody (1:10,000) (Clone hVIN-1, Sigma Aldrich Cat. V9131), respectively ...
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bioRxiv - Immunology 2023Quote: ... Antibody against Myb is from Sigma-Aldrich (Cat#05-175, Clone 1-1), Anti-ZEB2 was from Novus Biologicals (Cat#NBP1-82991 ...
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bioRxiv - Pathology 2024Quote: ... anti GABA antibodies (rabbit, Sigma, A2052, 1:5000; mouse; Sigma, A0310, 1:500), Clathrin antibody (Rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: EAAT2/GLT-1 1:5000 (cat#AB1783, Millipore), SCL711A/xCT 1:1000 (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... Primary antibodies (anti-Flag, 1:1000, Sigma, F3165; anti-USP30, 1:500, Sigma, HPA016952 ...
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bioRxiv - Cell Biology 2024Quote: Primary antibodies: β-Actin (Sigma-Aldrich, A1978; 1:5000 WB, 1:1000 IF), GAPDH (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... lysates were diluted 1:1 in RIPA buffer and precleared with protein A agarose beads (Millipore-Sigma) for 60 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... lysates were diluted 1:1 in RIPA buffer and precleared with protein A agarose beads (Millipore-Sigma) for 60 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... unbound primary antibodies were removed by washing in TBST and the blots were incubated with HRP-conjugated secondary antibodies for 1 hour at room temperature: goat anti-mouse IgG and IgM HRP conjugated antibodies (1:4000, Merck EMD Millipore, Ap130P), or goat anti-rabbit IgG antibody ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 were stained with an anti-His antibody (1:5,000) and secondary antibody conjugated with fluorescein (Sigma; F0257; 1:5,000). The solution was then centrifuged at 12,000 g for 5 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue sections incubated at room temperature overnight in primary antibody medium or control medium (1:100 primary anti β-catenin antibody from rabbit Sigma C2206 + 1:100 primary anti β-actin antibody from mouse Sigma A5441 + PBS pre-incubation medium ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to primary antibody solution containing monoclonal mouse anti-vesicular glutamate transporter 1 (VGlut1) antibody (1:750; Millipore, Burlington, MA) in blocking buffer and incubated overnight at room temperature ...
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bioRxiv - Genetics 2020Quote: ... using 1:1000 rabbit anti-Bestrophin1 antibody (LAgen Laboratories; Cat# 016-Best1-01) and 1:1000 mouse anti-Actin antibody (Millipore; Cat# MAB1501) as primary antibodies.
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated in PBS-T-milk for 1 h using 1:10000 dilutions of anti-rabbit or anti-mouse HRP-linked secondary antibodies (A0545 or A9044 Sigma antibodies, respectively) as required ...
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bioRxiv - Neuroscience 2022Quote: ... and 1 hour in secondary antibody (1:500 Alexa Fluor 568 donkey anti mouse antibody, Cat: A10037, Lot: 1917938)) with DAPI (1:1,000 Millipore Sigma, D9542-1MG). Tissue was washed 5 times for 5 minutes each in 1x PBS between each treatment and mounted on Fisherbrand Superfrost Plus slides ...
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bioRxiv - Microbiology 2023Quote: ... overnight at 4 °C following incubation with mouse anti-rAgApyrase antibody (1:10,000 in blocking buffer) or rabbit anti-tPA antibodies (1:2500) (Millipore-Sigma, Marlborough, MA, USA). Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase (1:2500 in blocking buffer ...