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Citations for Millipore Sigma :
1101 - 1150 of 10000+ citations for Synaptic Ras GTPase Activating Protein 1 Syngap1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was incubated with anti-HA antibody and immobilized on protein A Sepharose beads (Sigma). Beads washed 3 times with lysis buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples were transferred to nitrocellulose membranes (Amersham) upon which M2 Anti-FLAG mouse antibodies (Sigma) were used to visualize 3X-FLAG fusion peptides.
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bioRxiv - Cell Biology 2024Quote: ... then the antibody-protein complex was captured with EZview A/G beads (Millipore Sigma, Cat #: E3403). Beads were pelleted and washed with lysis buffer 3x ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained using the blocking solution containing rabbit polyclonal anti-TRPV2 antibody (1:200, ACC-039, Alomone labs) or rabbit polyclonal anti-FLAG antibody (1:100, F7425, Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Rabbit antibody against Parvalbumin (1:500, PA1-933, Thermo Fischer Scientific) and Mouse antibody against GAD67 (1:500, MAB5406, Sigma-Aldrich) in staining buffer (0.2% BSA ...
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bioRxiv - Physiology 2020Quote: ... sections were incubated with rabbit-anti-Iba1 antibody (1:3000, FUJIFILM Wako) or rabbit-anti-GFAP antibody (1:3000, Sigma-Aldrich) in staining solution overnight at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by overnight incubation with primary antibody overnight at 4 °C (1:100 rabbit-anti-HNF4A Cell signaling 3113S; 1:250 mouse anti-AFP antibody, Sigma A8452 ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked with PBS 1x-1% non-fat milk overnight at 4°C and incubated in primary antibody anti-Orco (1:500) and the secondary antibody Goat anti-rabbit IgG HRP conjugate (1:5000 Sigma-Aldrich) for 1 hour each ...
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bioRxiv - Microbiology 2023Quote: ... Incubation with primary antibodies (1 h, RT) was followed by washing and incubation with secondary antibodies and DAPI (25 µg ml-1, Sigma-Aldrich) (1 h ...
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bioRxiv - Microbiology 2023Quote: ... anti-p24 mouse monoclonal antibody (1: 500; MAB7360, R&D, Minneapolis, MN, USA) and anti-ZCCHC3 mouse monoclonal antibody (1: 500; SAB1408147, Sigma-Aldrich). The following secondary antibodies were used ...
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bioRxiv - Physiology 2023Quote: ... and incubated in anti-PEMT rabbit polyclonal antibody (1:1000, Bio-techne #NBP1-59580) or anti-α-TUBULIN mouse monoclonal antibody (1:10,000, Millipore-SIGMA #T5168) in 5% dairy milk ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were incubated for 1 hour using the following antibodies: 1:500 rabbit anti-H3S10ph (Sigma-Aldrich, Merck, 06-570), 1:500 mouse anticyclin B1 (BD Pharmingen ...
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bioRxiv - Neuroscience 2023Quote: ... A secondary antibody was incubated at room temperature for 1 hour (Donkey anti-rabbit IgG antibody: Sigma-Aldrich, SAB3700932; 1:100). Fusion protein ProteinA-Tn5 was prepared in-house (plasmid Addgene #124601 ...
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bioRxiv - Physiology 2023Quote: ... blots were stripped of connexin or ZO-1 antibodies and reprobed with an actin-specific monoclonal antibody (Ω-actin, 1:5000, Sigma). Intensities of Cx43 and ZO-1 bands were normalized to those of actin and quantified using the Las-3000 software.
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bioRxiv - Cell Biology 2024Quote: ... Cells were immunostained with rabbit anti-SON antibody (1:200 dilution, Novus) or mouse anti-SRRM2 antibody (1:200 dilution, Sigma Aldrich) for 1h at room temperature followed by 3 times wash with DPBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was then washed with TBS-T and incubated with 1:10000 goat anti-rabbit poly-HRP secondary antibody (Thermo, 32260) and 1:10,000 anti-β-actin-peroxidase antibody (Sigma, A3854) in 5% bovine serum album/TBS-T for 1 hr ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were exposed to either fluorophore-conjugated secondary antibodies (Li-Cor; 1:2000) or HRP-conjugated secondary antibodies (Sigma; 1:3000) in TBST with 5% milk for 30 min (Li-Cor ...
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bioRxiv - Microbiology 2024Quote: ... Incubation with primary antibodies (1 h, RT) was followed by washing and incubation with secondary antibodies and DAPI (25 µg ml-1, Sigma-Aldrich) (1 h ...
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bioRxiv - Biophysics 2024Quote: ... the membrane was blocked with 1% BSA with 0.05% Tween-20 and reacted with an anti-tau antibody (RTM38, 1:2500) and anti-β-actin antibody (1:10,000; Sigma-Aldrich) at RT for 1 h (or 4°C O/N) ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were washed in incubation buffer 6x5 mins and incubated in secondary antibodies for another 2 hours at room temperature (Goat anti-Chicken-IgY antibody, Alexa-488, 1:2000, Cat# A11039, ThermoFisher; Goat anti-Rabbit-IgG antibody, CF594, 1:2000, Cat# SAB4600234, Sigma Aldrich). The slides were washed in incubation buffer 6x5 mins followed by 2x5 mins PBS and covered by media and coverslips ...
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bioRxiv - Neuroscience 2024Quote: ... they were incubated for 2 h at room temperature with gentle shaking and overnight at 4 °C with one of the following primary antibodies: anti-beta-amyloid 1-42 antibody (beta-amyloid, RRID:AB_91677 Millipore #AB5078P, 1:1000), anti-tau antibody ...
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bioRxiv - Systems Biology 2024Quote: ... A secondary antibody was incubated at room temperature for 1 hour (Donkey anti-rabbit IgG antibody: Sigma-Aldrich, SAB3700932; 1:100). Fusion protein ProteinA-Tn5 was prepared in-house (plasmid Addgene #124601 ...
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bioRxiv - Cell Biology 2024Quote: ... Puromycin-tagged peptides were visualized by immunostaining with rabbit anti-puromycin antibody (1:500) and mouse anti-Flag M2 antibody (1:1000, Sigma, F1800), followed by the appropriate secondary antibodies ...
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bioRxiv - Genetics 2023Quote: ... The presence of the tagged protein was identified by immunoblotting using anti-FLAG antibody (F1804 monoclonal antibody, clone M2 in mouse, SLCK5688, Sigma Aldrich, Supplemental Fig 2F). Rescue was confirmed by measuring cilia length ...
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bioRxiv - Developmental Biology 2024Quote: ... Another set of Dynal Protein G Magnetic beads was coupled to anti-FLAG antibody by incubating washed Protein G beads with anti-FLAG antibody (Sigma Aldrich; Catalog number: F1804) with end-over-end rotation for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... The pharmacological agents used to block synaptic transmission were added to the external solution: Meclofenamic Acid (200 μM, Sigma), CNQX and DAP5 (20 μM ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were blocked in SureBloc (Lubio biosciences) for 1 hour followed by incubation with primary antibodies (anti Gpr133 antibody -Aviva Biosystems; anti Flag antibody: Sigma). HRP tagged Secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... and rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1) (1:350; Wako) to evaluate the inflammatory infiltrate and rabbit anti-myelin basic protein (MBP) (1:100; Millipore) to examine demyelination ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was incubated with primary antibody (mouse anti-FLAG antibody (Sigma, F1804; 1:1000); mouse anti-Tubulin (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used were as follows: Anti-Puromycin Antibody (1:2000, mouse, Millipore, cat# MABE343), Tau13 (1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibodies used were mouse monoclonal antibodies against actin (1:10000, Merck Millipore, MAB1501R), synaptophysin (1:5000 ...
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bioRxiv - Physiology 2022Quote: ... The primary antibodies used were as follows: chicken anti-GFP antibody (Sigma #G6539, 1:2,000), rabbit anti-RFP antibody (Medical & Biological Laboratories PM005 ...
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bioRxiv - Cell Biology 2022Quote: ... Hybridization was done with anti-HA-HRP antibody (monoclonal antibody from Sigma, dilution 1:7000) in TBST for 2h at room temperature ...
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bioRxiv - Zoology 2021Quote: ... The primary antibodies used were as follows: mouse anti-GFP antibody (Sigma G6539, 1:2000); rabbit anti-RFP antibody (Medical & Biological Laboratories PM005 ...
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bioRxiv - Genetics 2020Quote: ... Primary antibodies used were a mouse monoclonal anti-FLAG antibody (1:1,000; #F1804, Sigma-Aldrich), a polyclonal rabbit antibody against an epitope between residues 200-250 of PALB2 (1:2,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies used were anti-α-acetylated tubulin mouse monoclonal antibodies (1:250; Sigma, T7451) and anti-β-tubulin mouse monoclonal antibodies (1:250 ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies include: rabbit or mouse antibodies conjugated to horseradish peroxidase (HRP, Millipore; 1:5,000).
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bioRxiv - Neuroscience 2023Quote: ... The following antibodies were used: rabbit polyclonal antibodies (pAb) against KCC2 (1:1000, Sigma-Aldrich), ClC-2 (1:5000 ...
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bioRxiv - Microbiology 2022Quote: ... Primary antibodies used at 1:1,000 dilution were commercial rabbit anti-EPA antibodies (Millipore-Sigma), and O1 antibodies (20 ...
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bioRxiv - Microbiology 2022Quote: ... Primary antibodies used at 1:1,000 dilution were commercial rabbit anti-EPA antibodies (Millipore-Sigma), and O1 antibodies (20 ...
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bioRxiv - Neuroscience 2022Quote: ... followed by overnight incubation with primary antibodies (CDKL5- 1:1000, #HPA002847, Atlas Antibodies-Sigma Aldrich; NMDAR1-1:1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... Incubations with primary antibodies were performed in primary antibody solution: 1%BSA (Sigma-Aldrich, A2153), 10% donkey serum (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: The primary antibody used was mouse anti-FLAG M2 monoclonal antibody (1 : 5000, Sigma, F1804). Mouse anti-alpha tubulin (DM1A ...
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bioRxiv - Neuroscience 2024Quote: Primary antibodies used were mouse anti-acetylated tubulin antibody (1:100, Sigma-Aldrich, USA, #T7451), mouse anti-3A10 antibody (1:100 ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies were used for immunoblotting: anti-FLAG antibody (Sigma, F1804, 1:1000 overnight), anti-mouse HRP antibody (CST ...
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bioRxiv - Plant Biology 2024Quote: ... primary antibodies at 1:5,000 concentration followed by an anti-rabbit secondary antibody (Sigma A0545) at 1:30,000 ...
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bioRxiv - Neuroscience 2024Quote: ... NeuN primary antibody was added (mouse anti-NeuN monoclonal antibody, 1:1000, Millipore Sigma, MAB377), and incubated for 40 min at 4°C with gentle rocking ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was eluted with 4 mL of elution buffer and concentrated to 1 mL in a 3K protein concentrator (Millipore Sigma). Concentrated protein was purified by SEC as described above.
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bioRxiv - Cell Biology 2020Quote: ... otherwise all other protein labelling were using 1% Triton X100 (Sigma-Aldrich) in PBS permeabilization for 10 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Guanine nucleotide-binding protein subunit alpha-11 (1:1,000; Millipore 05-855R), Guanine nucleotide-binding protein G(q ...